BobuA.17672.a

Uncharacterized protein (BB_0024)

CENTER ID: BobuA.17672.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17672.a.B2.GE39135 mature protein 25 379
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.423
RefSeq: NP_212158.1
UniProt: O51055

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNQFLNSYF QLITTIFLIS SITIAAEEIT STLKVPNGFK VEIFLNNTIE KPRGITSDQD GNIFIGSGST FAYFVTKNRK IYTIAKTLQK PIGIDYWDNK LYISSVDKIY VVKNVKEEIN KSIKSHKDYT WKMQIFALLP KNNSQMHSGR YIKVDSKNNK LIVNIGSQHN VKIPPKKEAV ILSINLKTKK EEIVAFGVRN SVGFDFHPIS NEIYFSDNGQ DGLGDNIPPD EINVITEYKE HFGFPYVFGK NQKNYGFYNK APKNTKFIPS IYELPAHVAP LGIHFYRGNN FPKEYINKLF IAEHGSWNRS SPVGYKITTL DIDSKTRTAR NYKTFLYGFL KHDKSKFGRP VDIITYYDGS ILFTDDFGNK IYRVYYEKI
NT Sequence
atgaaaaatc aatttttaaa tagctatttt caattaatta caactatttt cttaatctca tctataacta ttgcagcaga agaaataaca agcacactaa aagttcctaa tggatttaaa gtcgaaattt ttttaaacaa tacaattgaa aaacctagag gaatcacaag cgatcaagat ggaaatatat tcataggatc tggaagcact tttgcatact ttgtaacaaa aaacagaaaa atttatacca tagcaaaaac cctgcaaaaa cctattggta ttgattattg ggataataaa ctctacatat cttctgtcga taaaatatat gtagttaaaa atgtaaaaga agaaattaat aaaagcataa aatcacataa agactataca tggaaaatgc aaatttttgc acttttgcca aaaaataatt ctcaaatgca ctcaggacgt tacattaaag tagattctaa aaataacaaa ttaatagtaa atataggatc ccagcacaat gttaaaattc ccccaaaaaa agaagcagta atccttagta ttaatttaaa aacaaaaaaa gaagaaatag tagcttttgg agtgagaaac tcagttgggt ttgattttca cccaattagc aatgaaatat attttagcga caatggccaa gacggattag gagacaacat tcccccagat gaaataaacg taataaccga atataaagaa cattttggat ttccctatgt gtttggaaaa aatcaaaaaa attacggttt ttataacaaa gcacccaaaa acactaagtt tatcccatct atttacgaac ttcccgcaca tgtagctcca cttggaatac acttttaccg gggaaataac tttccaaaag aatacataaa taaattattc atagcagaac acggctcgtg gaacagatct tctcctgttg gctacaaaat aacaacacta gacattgatt ctaaaaccag aacagcaaga aattacaaga cttttttata tggattttta aagcacgaca aatctaaatt tggacgccct gttgatataa tcacatatta tgacggttca attcttttta cagatgactt tggaaataaa atatacagag tttactacga aaagatttaa
Details for BobuA.17672.a.B2.GE39135
HARVESTED ON: 5/6/2015
SEQUENCED ON: 5/6/2015
EXPECTED MW: 42kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA XEEITSTLKV PNGFKXEXXX NNTIEKPRGI TSDQDGNIFI GSGSTFAYFV TKNRKIYTIA KTLQKPIGID YWDNKLYISS VDKIYVVKNV KEEINKSIKS HKDYTWKMQI FALLPKNNSQ MHSGRYIKVD SKNNKLIVNI GSQHNVKIPP KKEAVILSIN LKTKKEEIXA FGVRNSVGFD FHPISNEIYF SVNGQDGLGD NIPPDEINVI TEYKEHFGFP YVFGKNQKNY GFYNKAPKNT KFIPSIYELP AHVAPLGIHF YRGNNFPKEY INKLFIAEHG SWNRSSPVGY KITTLDIDSK TRTARNYKTF LYGFLKHDKS KFGRPVDIIT YYDGSILFTD DFGN
Validated NT Sequence
ttatttccaa agtcatctgt aaaaagaatt gaaccgtcat aatatgtgat tatatcaaca gggcgtccaa atttagattt gtcgtgcttt aaaaatccat ataaaaaagt cttgtaattt cttgctgttc tggttttaga atcaatgtct agtgttgtta ttttgtagcc aacaggagaa gatctgttcc acgagccgtg ttctgctatg aataatttat ttatgtattc ttttggaaag ttatttcccc ggtaaaagtg tattccaagt ggagctacat gtgcgggaag ttcgtaaata gatgggataa acttagtgtt tttgggtgct ttgttataaa aaccgtaatt tttttgattt tttccaaaca catagggaaa tccaaaatgt tctttatatt cggttattac gtttatttca tctgggggaa tgttgtctcc taatccgtct tggccattga cgctaaaata tatttcattg ctaattgggt gaaaatcaaa cccaactgag tttctcactc caaaagctan aatttcttct ttttttgttt ttaaattaat actaaggatt actgcttctt tttttggggg aattttaaca ttgtgctggg atcctatatt tactattaat ttgttatttt tagaatctac tttaatgtaa cgtcctgagt gcatttgaga attatttttt ggcaaaagtg caaaaatttg cattttccat gtatagtctt tatgtgattt tatgctttta ttaatttctt cttttacatt tttaactaca tatattttat cgacagaaga tatgtagagt ttattatccc aataatcaat accaataggt ttttgcaggg tttttgctat ggtataaatt tttctgtttt ttgttacaaa gtatgcaaaa gtgcttccag atcctatgaa tatatttcca tcttgatcgc ttgtgattcc tctaggtttt tcaattgtat tgttnnnann natttcgnnt ttaaatccat taggaacttt tagtgtgctt gttatttctt ctnntgccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHAA EEITSTLKVP NGFKVEIFLN NTIEKPRGIT SDQDGNIFIG SGSTFAYFVT KNRKIYTIAK TLQKPIGIDY WDNKLYISSV DKIYVVKNVK EEINKSIKSH KDYTWKMQIF ALLPKNNSQM HSGRYIKVDS KNNKLIVNIG SQHNVKIPPK KEAVILSINL KTKKEEIVAF GVRNSVGFDF HPISNEIYFS DNGQDGLGDN IPPDEINVIT EYKEHFGFPY VFGKNQKNYG FYNKAPKNTK FIPSIYELPA HVAPLGIHFY RGNNFPKEYI NKLFIAEHGS WNRSSPVGYK ITTLDIDSKT RTARNYKTFL YGFLKHDKSK FGRPVDIITY YDGSILFTDD FGNKIYRVYY EKI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcagcag aagaaataac aagcacacta aaagttccta atggatttaa agtcgaaatt tttttaaaca atacaattga aaaacctaga ggaatcacaa gcgatcaaga tggaaatata ttcataggat ctggaagcac ttttgcatac tttgtaacaa aaaacagaaa aatttatacc atagcaaaaa ccctgcaaaa acctattggt attgattatt gggataataa actctacata tcttctgtcg ataaaatata tgtagttaaa aatgtaaaag aagaaattaa taaaagcata aaatcacata aagactatac atggaaaatg caaatttttg cacttttgcc aaaaaataat tctcaaatgc actcaggacg ttacattaaa gtagattcta aaaataacaa attaatagta aatataggat cccagcacaa tgttaaaatt cccccaaaaa aagaagcagt aatccttagt attaatttaa aaacaaaaaa agaagaaata gtagcttttg gagtgagaaa ctcagttggg tttgattttc acccaattag caatgaaata tattttagcg acaatggcca agacggatta ggagacaaca ttcccccaga tgaaataaac gtaataaccg aatataaaga acattttgga tttccctatg tgtttggaaa aaatcaaaaa aattacggtt tttataacaa agcacccaaa aacactaagt ttatcccatc tatttacgaa cttcccgcac atgtagctcc acttggaata cacttttacc ggggaaataa ctttccaaaa gaatacataa ataaattatt catagcagaa cacggctcgt ggaacagatc ttctcctgtt ggctacaaaa taacaacact agacattgat tctaaaacca gaacagcaag aaattacaag acttttttat atggattttt aaagcacgac aaatctaaat ttggacgccc tgttgatata atcacatatt atgacggttc aattcttttt acagatgact ttggaaataa aatatacaga gtttactacg aaaagatttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat