BobuA.17584.a

Flagellar motor switch protein (FliG-1) (BB_0221)

CENTER ID: BobuA.17584.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17584.a.A1.PW30710 Full length( BobuA.17584.a ) 1 408 unavailable
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_136479
PATRIC ID: fig|224326.49.peg.619
RefSeq: NP_212355.1
UniProt: O51239
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVMQDPRLS KYKNAKNLSK KTVEEIDTLS LNKNFSDSEI QGSMLRSWIS LIKGNKNKTP VEPKSNNINE LKPGFIRKES KVTKIAKYFL AIGLEKSAKI MSELDDSDII LITREISKIS YIAPEEKEHI IKEFEELLRN QKRYVKVDDN FAYELLNKTL GKSKAKSIYK KVTGNDIFIP FDYLSQIEHE QLWALIKDEN IKTLVILYNY LNKDQKKYVF SMLEEEDRRK FVKDLVKPRQ LSMETIEAIS NKLKDRFKMQ GNLKTDKLDG SKILVDILSY MDFNEEKNLL NNINMRDLNP VVDSEIREKI FDINVILRIM DNDLHNILRE FTDKEIAIII KDKADEVRDK MLSNVSKRRK EIILDEEAFL GKISKKDLKT MTTAFVNYIK NLTLKGDLII YRKNEEFI
NT Sequence
ATGAAAGTTA TGCAGGATCC TAGGCTTTCC AAGTATAAAA ATGCTAAAAA TTTAAGCAAA AAAACCGTTG AAGAAATTGA TACTTTAAGT TTAAATAAAA ATTTTTCCGA TTCAGAGATT CAAGGCTCTA TGCTTAGATC TTGGATAAGC CTTATTAAAG GAAATAAAAA TAAAACACCT GTCGAGCCTA AATCCAACAA TATTAATGAG TTAAAGCCAG GGTTTATTCG CAAAGAGAGT AAGGTCACAA AGATTGCAAA ATATTTTTTG GCTATTGGAC TTGAAAAATC TGCTAAGATT ATGTCTGAGC TTGATGATTC GGATATAATC TTAATTACCA GAGAAATTTC AAAGATTAGC TATATTGCTC CAGAAGAAAA AGAGCATATT ATTAAAGAAT TTGAAGAGTT ACTAAGAAAT CAAAAAAGGT ATGTTAAGGT TGATGATAAC TTTGCTTATG AGTTATTAAA TAAAACGTTA GGAAAATCTA AGGCAAAATC AATTTATAAA AAAGTTACTG GTAATGATAT TTTTATTCCT TTTGATTATT TGTCTCAAAT TGAGCATGAA CAACTTTGGG CATTAATTAA GGATGAGAAT ATTAAAACGC TTGTTATATT ATATAATTAT TTAAATAAAG ATCAAAAAAA ATATGTTTTT TCAATGCTAG AAGAAGAAGA TAGAAGAAAA TTTGTAAAAG ATCTTGTCAA ACCAAGGCAA TTGAGCATGG AGACAATAGA GGCAATTTCA AATAAGCTTA AGGATAGATT TAAGATGCAG GGGAATCTTA AGACAGATAA ACTTGATGGA TCTAAGATAC TTGTTGATAT TTTAAGCTAT ATGGATTTTA ATGAAGAGAA AAATTTGCTT AATAATATTA ATATGCGGGA TTTAAATCCT GTTGTGGACA GTGAAATTCG AGAAAAAATA TTTGATATCA ATGTAATATT GAGAATAATG GATAATGACC TTCATAATAT TTTAAGAGAG TTTACAGACA AAGAGATTGC AATTATTATT AAGGATAAAG CTGATGAGGT TAGAGATAAA ATGCTTTCAA ATGTTTCAAA AAGGCGCAAA GAAATCATTT TAGACGAAGA AGCTTTTTTA GGAAAAATAA GTAAAAAAGA TTTAAAAACT ATGACAACAG CTTTTGTTAA TTATATTAAA AATTTAACTT TAAAAGGCGA TTTAATAATA TATAGAAAAA ATGAGGAATT TATT
Details for BobuA.17584.a.A1.PW30710
PURIFICATION DATe: 2/5/2012
CONCENTRATION: 6.85mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 43
Protocol Notes
notes unavailable
Validated AA Sequence
MLEEEDRRKF VKDLVKPRQL SMETIEAISN KLKDRFKMQG NLKTDKLDGS KILVDILSYM DFNEEKNLLN NINMRDLNPV VDSEIREKIF DINVILRIMD NDLHNILREF TDKEIAIIIK DKADEVRDKM LSNVSKRRKE IILDEEAFLG KISKKDLKTM TTAFVNYIKN LTLKGDLIIY RKNEEFI
Validated NT Sequence
cnttnnnnct ttgtnagcag ccggatcctc gagaagcttg gctgcagaac ttgttcgtgc tgtttattaa ataaattcct cattttttct atatattatt aaatcgcctt ttaaagttaa atttttaata taattaacaa aagctgttgt catagttttt aaatcttttt tacttatttt tcctaaaaaa gcttcttcgt ctaaaatgat ttctttgcgc ctttttgaaa catttgaaag cattttatct ctaacctcat cagctttatc cttaataata attgcaatct ctttgtctgt aaactctctt aaaatattat gaaggtcatt atccattatt ctcaatatta cattgatatc aaatattttt tctcgaattt cactgtccac aacaggattt aaatcccgca tattaatatt attaagcaaa tttttctctt cattaaaatc catatagctt aaaatatcaa caagtatctt agatccatca agtttatctg tcttaagatt cccctgcatc ttaaatctat ccttaagctt atttgaaatt gcctctattg tctccatgct caattgcctt ggtttgacaa gatcttttac aaattttctt ctatcttctt cttctagcat tgaaaaaaca tatttttttt gatctttatt taaataatta tataatataa caagcgtttt aatattctca tccttaatta atgcccaaag ttgttcatgc tcaatttgag acnnnnatca aaaggaataa aaatatcatt accagtaact tttttataaa ttgattttgc cttagatttt cctaacgttt tatttaataa ctcataagca aagttatcat caaccttaac ataccttttt tgatttctta ataactcttc aaattcttta ataatatgct ctttttcntc tggagcaata tagctaatct ttgaaatttc tctggnaatt aanattatat ccnaancatc aagctcagac ntnatcnnag canatttttc angtccnnna ncnnaaaann tttgnannnt nnngannnta ctctcntngn nannnaccnn gnntnnntcn nttnngnncg acnggnnntt tnnnnnnant nnnnnnnnnn nnnnncnana nctnnncnnn nnnnnnnann nnnnnnnnnn nnnnntnnnt nnnnnnnnnn nnnnnnnnnn nnttt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKVMQDPRL SKYKNAKNLS KKTVEEIDTL SLNKNFSDSE IQGSMLRSWI SLIKGNKNKT PVEPKSNNIN ELKPGFIRKE SKVTKIAKYF LAIGLEKSAK IMSELDDSDI ILITREISKI SYIAPEEKEH IIKEFEELLR NQKRYVKVDD NFAYELLNKT LGKSKAKSIY KKVTGNDIFI PFDYLSQIEH EQLWALIKDE NIKTLVILYN YLNKDQKKYV FSMLEEEDRR KFVKDLVKPR QLSMETIEAI SNKLKDRFKM QGNLKTDKLD GSKILVDILS YMDFNEEKNL LNNINMRDLN PVVDSEIREK IFDINVILRI MDNDLHNILR EFTDKEIAII IKDKADEVRD KMLSNVSKRR KEIILDEEAF LGKISKKDLK TMTTAFVNYI KNLTLKGDLI IYRKNEEFI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAGTTATG CAGGATCCTA GGCTTTCCAA GTATAAAAAT GCTAAAAATT TAAGCAAAAA AACCGTTGAA GAAATTGATA CTTTAAGTTT AAATAAAAAT TTTTCCGATT CAGAGATTCA AGGCTCTATG CTTAGATCTT GGATAAGCCT TATTAAAGGA AATAAAAATA AAACACCTGT CGAGCCTAAA TCCAACAATA TTAATGAGTT AAAGCCAGGG TTTATTCGCA AAGAGAGTAA GGTCACAAAG ATTGCAAAAT ATTTTTTGGC TATTGGACTT GAAAAATCTG CTAAGATTAT GTCTGAGCTT GATGATTCGG ATATAATCTT AATTACCAGA GAAATTTCAA AGATTAGCTA TATTGCTCCA GAAGAAAAAG AGCATATTAT TAAAGAATTT GAAGAGTTAC TAAGAAATCA AAAAAGGTAT GTTAAGGTTG ATGATAACTT TGCTTATGAG TTATTAAATA AAACGTTAGG AAAATCTAAG GCAAAATCAA TTTATAAAAA AGTTACTGGT AATGATATTT TTATTCCTTT TGATTATTTG TCTCAAATTG AGCATGAACA ACTTTGGGCA TTAATTAAGG ATGAGAATAT TAAAACGCTT GTTATATTAT ATAATTATTT AAATAAAGAT CAAAAAAAAT ATGTTTTTTC AATGCTAGAA GAAGAAGATA GAAGAAAATT TGTAAAAGAT CTTGTCAAAC CAAGGCAATT GAGCATGGAG ACAATAGAGG CAATTTCAAA TAAGCTTAAG GATAGATTTA AGATGCAGGG GAATCTTAAG ACAGATAAAC TTGATGGATC TAAGATACTT GTTGATATTT TAAGCTATAT GGATTTTAAT GAAGAGAAAA ATTTGCTTAA TAATATTAAT ATGCGGGATT TAAATCCTGT TGTGGACAGT GAAATTCGAG AAAAAATATT TGATATCAAT GTAATATTGA GAATAATGGA TAATGACCTT CATAATATTT TAAGAGAGTT TACAGACAAA GAGATTGCAA TTATTATTAA GGATAAAGCT GATGAGGTTA GAGATAAAAT GCTTTCAAAT GTTTCAAAAA GGCGCAAAGA AATCATTTTA GACGAAGAAG CTTTTTTAGG AAAAATAAGT AAAAAAGATT TAAAAACTAT GACAACAGCT TTTGTTAATT ATATTAAAAA TTTAACTTTA AAAGGCGATT TAATAATATA TAGAAAAAAT GAGGAATTTA TTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA