BobuA.17514.a

Flagellar motor switch protein FliM (BB_0278)

CENTER ID: BobuA.17514.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17514.a.A1.GE32512 Full length( BobuA.17514.a ) 1 352
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.677
RefSeq: NP_212412.1
UniProt: Q57511

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANNPGALSQ DDIDSLLESI NSSESLSLDE SLSNVISSPT GKKQKVKVYD FKRPDKFSKE QVRTVSSFHE AFARYTTTSL SALLRKMVHV HVASVDQLTY EEFIRSIPNP TTLAIINMDP LKGSAIFEVD PTIAFAIVDR LFGGDGDTIK DKSRDLTEIE QSVMESVIIR ILANMREAWS QVVDLRPRFG HIEVNPQFAQ IVPPTEMIIL VTLEVKIGKV EGLMNFCLPY ITIEPIVSKL STRYWHSLIG VGTTSENLDA LREKLENTAM PLVAEIGEVK LKVREILSLD KGDVLNLESS LINKDLTLKV GTKEKFKCRM GLMGNKVSVQ ITEKIGDIKG FDLLKELTEE VE
NT Sequence
ATGGCAAACA ATCCAGGAGC TTTATCTCAA GATGATATAG ATAGTCTTTT AGAGTCTATC AACTCGTCTG AAAGTTTATC CTTAGACGAA TCCCTTTCTA ATGTTATATC TAGCCCTACA GGCAAAAAGC AAAAAGTTAA GGTTTATGAC TTTAAAAGGC CAGATAAATT TTCAAAAGAG CAGGTTAGAA CAGTTTCAAG TTTTCACGAG GCATTTGCCA GATATACTAC AACTTCACTT TCTGCTCTTT TAAGAAAAAT GGTTCATGTT CACGTAGCCT CAGTTGATCA ATTAACCTAT GAAGAGTTTA TTAGGTCAAT ACCCAACCCT ACAACTTTAG CAATAATAAA TATGGACCCT CTTAAAGGAT CTGCTATTTT TGAAGTTGAT CCAACAATAG CATTTGCTAT TGTAGATAGG CTTTTTGGGG GTGATGGAGA TACGATTAAA GATAAAAGTA GAGATTTAAC AGAAATTGAG CAGTCTGTTA TGGAAAGTGT TATTATTCGT ATACTTGCTA ATATGAGAGA GGCTTGGTCT CAAGTTGTTG ATTTAAGGCC AAGATTTGGT CATATTGAGG TCAATCCACA GTTTGCTCAA ATAGTTCCCC CTACAGAAAT GATTATTTTA GTAACTCTTG AGGTTAAAAT AGGAAAAGTA GAAGGGCTTA TGAATTTTTG TTTGCCTTAT ATTACCATAG AGCCTATTGT TTCAAAATTA TCAACAAGAT ATTGGCATTC TTTGATTGGG GTTGGAACTA CTAGCGAGAA TCTTGATGCT TTGCGTGAAA AGTTGGAAAA TACAGCGATG CCTTTGGTAG CAGAAATAGG GGAAGTTAAA CTTAAAGTAA GAGAAATTTT ATCGCTTGAC AAAGGTGATG TTTTAAATCT TGAATCTTCT CTAATAAATA AAGATTTAAC TTTGAAAGTT GGTACCAAAG AAAAGTTTAA ATGCAGAATG GGTTTAATGG GAAATAAAGT TTCAGTGCAA ATTACAGAAA AAATTGGAGA TATAAAAGGT TTTGATTTAT TAAAAGAGCT TACAGAAGAG GTTGAA
Details for BobuA.17514.a.A1.GE32512
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 79
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMANNPGALS QDDIDSLLES INSSESLSLD ESLSNVISSP TGKKQKVKVY DFKRPDKFSK EQVRTVSSFH EAFARYTTTS LSALLRKMVH VHVASVDQLT YEEFIRSIPN PTTLAIINMD PLKGSAIFEV DPTIAFAIVD RLFGGDGDTI KDKSRDLTEI EQSVMESVII RILANMREAW SQVVDLRPRF GHIEVNPQFA QIVPPTEMII LVTLEXKIGK VEGLMNFCLP YITIEPIVSK LSTRYWXSLI GVGTTSENLD ALREKLENTA MPLVAX
Validated NT Sequence
gnnntnnctn tntannnagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcaaa caatccagga gctttatctc aagatgatat agatagtctt ttagagtcta tcaactcgtc tgaaagttta tccttagacg aatccctttc taatgttata tctagcccta caggcaaaaa gcaaaaagtt aaggtttatg actttaaaag gccagataaa ttttcaaaag agcaggttag aacagtttca agttttcacg aggcatttgc cagatatact acaacttcac tttctgctct tttaagaaaa atggttcatg ttcacgtagc ctcagttgat caattaacct atgaagagtt tattaggtca atacccaacc ctacaacttt agcaataata aatatggacc ctcttaaagg atctgctatt tttgaagttg atccaacaat agcatttgct attgtagata ggctttttgg gggtgatgga gatacgatta aagataaaag tagagattta acagaaattg agcagtctgt tatggaaagt gttattattc gtatacttgc taatatgaga gaggcttggt ctcaagttgt tgatttaagg ccaagatttg gtcatattga ggtcaatcca cagtttgctc aaatagttcc ccctacagaa atgattattt tagtaactct tgaggntaaa ataggaaaag tagaagggct tatgaatttt tgtttgcctt atattaccat agagcctatt gtttcaaaat tatcaacaag atattggcan tctttgattg gggttggaac tactagcgag aatcttgatg ctttgcgtga aaagttggaa aatacagcga tgcctttggt agcagantag gggaagttaa acttnnntaa nagaaatttt atcgctngan annngangtt taatctgatn tntcnataat nannattant ttgaannnnn cnangnnnnt naangcnnan ggntnnngnn annaagntnc nnngcnantn nnnaaaannn naannnnnnn ntnnntnnnn nannannntn nnnnnnnnan nannaannnn nnnnnnnann nnnnnnnnnn nnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMANNPGALS QDDIDSLLES INSSESLSLD ESLSNVISSP TGKKQKVKVY DFKRPDKFSK EQVRTVSSFH EAFARYTTTS LSALLRKMVH VHVASVDQLT YEEFIRSIPN PTTLAIINMD PLKGSAIFEV DPTIAFAIVD RLFGGDGDTI KDKSRDLTEI EQSVMESVII RILANMREAW SQVVDLRPRF GHIEVNPQFA QIVPPTEMII LVTLEVKIGK VEGLMNFCLP YITIEPIVSK LSTRYWHSLI GVGTTSENLD ALREKLENTA MPLVAEIGEV KLKVREILSL DKGDVLNLES SLINKDLTLK VGTKEKFKCR MGLMGNKVSV QITEKIGDIK GFDLLKELTE EVE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaaacaat ccaggagctt tatctcaaga tgatatagat agtcttttag agtctatcaa ctcgtctgaa agtttatcct tagacgaatc cctttctaat gttatatcta gccctacagg caaaaagcaa aaagttaagg tttatgactt taaaaggcca gataaatttt caaaagagca ggttagaaca gtttcaagtt ttcacgaggc atttgccaga tatactacaa cttcactttc tgctctttta agaaaaatgg ttcatgttca cgtagcctca gttgatcaat taacctatga agagtttatt aggtcaatac ccaaccctac aactttagca ataataaata tggaccctct taaaggatct gctatttttg aagttgatcc aacaatagca tttgctattg tagataggct ttttgggggt gatggagata cgattaaaga taaaagtaga gatttaacag aaattgagca gtctgttatg gaaagtgtta ttattcgtat acttgctaat atgagagagg cttggtctca agttgttgat ttaaggccaa gatttggtca tattgaggtc aatccacagt ttgctcaaat agttccccct acagaaatga ttattttagt aactcttgag gttaaaatag gaaaagtaga agggcttatg aatttttgtt tgccttatat taccatagag cctattgttt caaaattatc aacaagatat tggcattctt tgattggggt tggaactact agcgagaatc ttgatgcttt gcgtgaaaag ttggaaaata cagcgatgcc tttggtagca gaaatagggg aagttaaact taaagtaaga gaaattttat cgcttgacaa aggtgatgtt ttaaatcttg aatcttctct aataaataaa gatttaactt tgaaagttgg taccaaagaa aagtttaaat gcagaatggg tttaatggga aataaagttt cagtgcaaat tacagaaaaa attggagata taaaaggttt tgatttatta aaagagctta cagaagaggt tgaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa