CENTER ID: | BobuA.17504.a |
ORGANISM: | Borrelia burgdorferi B31 |
ASSOCIATED DISEASE: | Lyme disease |
CURRENT STATUS: | purified |
COMMUNITY REQUEST: | False |
NIH RISK GROUP: | 2 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
I |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
BobuA.17504.a.A1.GE30817 | Full length( BobuA.17504.a ) | 1 | 176 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
BobuA.17504.a.A1.PS01010 | Full length( BobuA.17504.a ) | 1 | 176 |
RESOURCE | REFERENCE ID |
---|---|
BV-BRC: | fig|224326.49.peg.710 |
RefSeq: | NP_212446.1 |
UniProt: | O51292 |
HARVESTED ON: | 11/16/2010 |
SEQUENCED ON: | 11/23/2010 |
EXPECTED MW: | 22kDa |
OBSERVED MW: | 21kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | High Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass with sequence variation |
PERCENT IDENTITY: | 99 |
PERCENT COVERAGE: | 98 |
PURIFICATION DATe: | 3/15/2011 |
CONCENTRATION: | 36.65mg/ml |
OBSERVED MW: | data unavailable |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 4 |
VIAL VOLUME: | 100µl |
PERCENT IDENTITY: | 99 |
PERCENT COVERAGE: | 98 |