BobuA.17489.a

Flagellar hook-associated protein 2 (BB_0149)

CENTER ID: BobuA.17489.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17489.a.A1.GE32509 Full length( BobuA.17489.a ) 1 665
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17489.a.A1.PS01134 Full length( BobuA.17489.a ) 1 665

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.547
RefSeq: NP_212283.1
UniProt: O51173

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASGFFVPGL ESKYNTKEIR ESMLKSDKAK IDSSFKKLES LEQEKSAWQL INRKISTLNS LAKELTSLNS PFNLMSGNSS NSEVLTLSTR YGSKNETHKL IVDQIASADV FLSSNFDPKK VTIPEGDYIF LVGKKEINVK SNGNIDLLVK DINNKGKGFL SAKIVKSDKN GNSRFVLQSL KEGKENKLVI KGEGLSFAKQ IGILSELKTN FNPNLSDIVV NQSSSNNKLA FENNGLVLNP LSEVSIEIPE DIEITSRSKI KFEVKYFDTG LEEPDSKIIF NPGGATFKDA KVESEDSVVD LGSDLKTPLE KKYIQMNMVK ICSKEGSLEL PLINISNNFE EVEVDVGALS NLEEINIENK ANNKVIVISN VEIFDPKNRD GHLPINAKSF AENAKIKFDG VDVERDSNVI NDLVPNVTLS LKKPSSDMVE AKIEPDYEGI KRVLLDFIGA YNEVLAEINI VSSNEDQPNN QKSNIVEELT YLSDSQKEEA YKNLGILRSE FLLKNLKSKL ESIIFKPYVT SDPNFSIINQ MGVFTNSISS SGGLSRYLRL DEKKFDESIR NNIDNVRELF LYDLNGDRVY DNGIAKMLGD CLSPLVASGG VIYNKIKNYD LKIFNQKNKV EDYKKKYEDR ERKVEGELNT LDFTVKRMKD QENTLKAFDF NQRNK
NT Sequence
TTGGCATCAG GATTTTTTGT TCCTGGACTT GAGAGTAAGT ACAATACTAA AGAAATTCGT GAATCCATGC TTAAGTCCGA TAAGGCTAAA ATTGATTCTT CTTTTAAGAA ACTTGAATCT TTAGAGCAAG AGAAAAGTGC CTGGCAGTTA ATTAATAGAA AAATCTCTAC TTTAAATTCT CTTGCAAAAG AACTTACATC ACTCAACAGT CCTTTTAATC TAATGTCAGG AAATTCTAGT AATAGCGAAG TTTTAACTTT GTCTACTAGA TATGGATCTA AGAATGAGAC TCATAAATTA ATTGTTGATC AAATAGCGTC AGCTGATGTG TTTTTGTCTT CAAATTTTGA TCCTAAAAAA GTTACAATCC CAGAGGGAGA TTATATATTT TTAGTTGGCA AGAAAGAAAT TAATGTAAAA AGTAATGGCA ACATTGATTT ACTTGTGAAG GATATTAATA ACAAGGGAAA GGGCTTTTTA TCTGCAAAAA TAGTGAAAAG TGATAAAAAT GGAAATAGTC GTTTTGTTTT GCAATCCTTA AAGGAGGGCA AAGAAAACAA GCTTGTTATC AAAGGGGAGG GATTGTCTTT TGCTAAGCAA ATTGGAATTT TAAGTGAGCT TAAAACCAAT TTTAATCCTA ATCTTTCAGA TATTGTTGTA AATCAATCTA GCAGCAACAA TAAACTTGCT TTTGAGAACA ATGGTCTTGT TTTAAATCCG CTTTCAGAAG TATCAATTGA AATTCCTGAA GATATTGAAA TTACATCTAG GAGTAAGATT AAATTTGAAG TTAAGTATTT TGATACAGGC TTGGAAGAGC CTGATAGTAA GATTATTTTT AATCCCGGAG GGGCTACATT TAAGGATGCA AAAGTTGAGA GTGAAGATAG TGTAGTTGAT CTTGGATCTG ATTTAAAAAC CCCTTTGGAA AAAAAATATA TTCAAATGAA TATGGTTAAA ATATGTAGCA AGGAAGGTTC TTTGGAGCTT CCTTTAATAA ATATTTCAAA TAATTTTGAA GAAGTTGAAG TTGATGTTGG AGCTCTTTCT AATTTGGAAG AAATAAATAT TGAAAATAAA GCAAATAATA AAGTAATTGT GATTAGCAAT GTTGAAATTT TTGATCCAAA AAATAGAGAT GGTCATTTGC CAATAAATGC TAAAAGTTTT GCTGAAAATG CAAAAATTAA ATTTGATGGA GTAGATGTTG AGAGAGATTC AAATGTTATA AATGATTTGG TTCCAAATGT GACATTAAGT TTAAAAAAAC CCTCAAGTGA TATGGTTGAG GCTAAAATTG AACCTGATTA TGAGGGGATT AAGAGGGTTC TTTTAGATTT TATTGGTGCT TATAATGAGG TTCTTGCTGA GATTAATATT GTAAGCTCTA ATGAAGATCA GCCTAATAAT CAAAAGTCTA ATATAGTTGA AGAGCTAACT TATCTTAGTG ATTCTCAAAA AGAAGAGGCT TATAAAAATT TAGGTATTCT AAGGTCTGAA TTTTTATTAA AAAATCTTAA GTCCAAGCTA GAGTCAATAA TTTTTAAGCC TTATGTTACT AGTGATCCTA ATTTTTCAAT AATTAATCAG ATGGGAGTTT TTACAAATTC CATTTCTTCT TCTGGTGGAC TTTCTAGATA TTTAAGACTT GATGAGAAAA AGTTTGATGA ATCAATTCGT AATAATATTG ATAATGTTAG AGAGCTTTTT TTATATGATC TTAATGGTGA CAGAGTGTAT GATAATGGAA TTGCTAAAAT GCTAGGAGAT TGTCTGTCGC CTCTTGTGGC TTCCGGAGGA GTTATTTATA ATAAAATAAA GAATTACGAC TTGAAAATTT TTAATCAAAA AAATAAAGTT GAAGATTATA AAAAGAAGTA CGAAGATAGA GAGAGAAAAG TGGAAGGTGA ACTTAATACC TTGGATTTTA CCGTTAAGCG CATGAAAGAT CAAGAAAATA CATTAAAGGC TTTTGATTTT AATCAAAGAA ATAAA
Details for BobuA.17489.a.A1.GE32509
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 77kDa
OBSERVED MW: 77kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 64
PERCENT COVERAGE: 10
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLASGFFVL ATQNPIEQEG TYNLPESQLD RFLLKVNVHY PSVQDEVRLL ILIK
Validated NT Sequence
catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgttg gcatcaggat tttttgttct tgccactcaa aatccaatag agcaagaggg gacttataat ttgccagaat cccaacttga tagattttta ttaaaagtta atgttcatta tccatcagtg caagatgagg taaggctttt gattttaatc aaagaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLASGFFVP GLESKYNTKE IRESMLKSDK AKIDSSFKKL ESLEQEKSAW QLINRKISTL NSLAKELTSL NSPFNLMSGN SSNSEVLTLS TRYGSKNETH KLIVDQIASA DVFLSSNFDP KKVTIPEGDY IFLVGKKEIN VKSNGNIDLL VKDINNKGKG FLSAKIVKSD KNGNSRFVLQ SLKEGKENKL VIKGEGLSFA KQIGILSELK TNFNPNLSDI VVNQSSSNNK LAFENNGLVL NPLSEVSIEI PEDIEITSRS KIKFEVKYFD TGLEEPDSKI IFNPGGATFK DAKVESEDSV VDLGSDLKTP LEKKYIQMNM VKICSKEGSL ELPLINISNN FEEVEVDVGA LSNLEEINIE NKANNKVIVI SNVEIFDPKN RDGHLPINAK SFAENAKIKF DGVDVERDSN VINDLVPNVT LSLKKPSSDM VEAKIEPDYE GIKRVLLDFI GAYNEVLAEI NIVSSNEDQP NNQKSNIVEE LTYLSDSQKE EAYKNLGILR SEFLLKNLKS KLESIIFKPY VTSDPNFSII NQMGVFTNSI SSSGGLSRYL RLDEKKFDES IRNNIDNVRE LFLYDLNGDR VYDNGIAKML GDCLSPLVAS GGVIYNKIKN YDLKIFNQKN KVEDYKKKYE DRERKVEGEL NTLDFTVKRM KDQENTLKAF DFNQRNK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggcatca ggattttttg ttcctggact tgagagtaag tacaatacta aagaaattcg tgaatccatg cttaagtccg ataaggctaa aattgattct tcttttaaga aacttgaatc tttagagcaa gagaaaagtg cctggcagtt aattaataga aaaatctcta ctttaaattc tcttgcaaaa gaacttacat cactcaacag tccttttaat ctaatgtcag gaaattctag taatagcgaa gttttaactt tgtctactag atatggatct aagaatgaga ctcataaatt aattgttgat caaatagcgt cagctgatgt gtttttgtct tcaaattttg atcctaaaaa agttacaatc ccagagggag attatatatt tttagttggc aagaaagaaa ttaatgtaaa aagtaatggc aacattgatt tacttgtgaa ggatattaat aacaagggaa agggcttttt atctgcaaaa atagtgaaaa gtgataaaaa tggaaatagt cgttttgttt tgcaatcctt aaaggagggc aaagaaaaca agcttgttat caaaggggag ggattgtctt ttgctaagca aattggaatt ttaagtgagc ttaaaaccaa ttttaatcct aatctttcag atattgttgt aaatcaatct agcagcaaca ataaacttgc ttttgagaac aatggtcttg ttttaaatcc gctttcagaa gtatcaattg aaattcctga agatattgaa attacatcta ggagtaagat taaatttgaa gttaagtatt ttgatacagg cttggaagag cctgatagta agattatttt taatcccgga ggggctacat ttaaggatgc aaaagttgag agtgaagata gtgtagttga tcttggatct gatttaaaaa cccctttgga aaaaaaatat attcaaatga atatggttaa aatatgtagc aaggaaggtt ctttggagct tcctttaata aatatttcaa ataattttga agaagttgaa gttgatgttg gagctctttc taatttggaa gaaataaata ttgaaaataa agcaaataat aaagtaattg tgattagcaa tgttgaaatt tttgatccaa aaaatagaga tggtcatttg ccaataaatg ctaaaagttt tgctgaaaat gcaaaaatta aatttgatgg agtagatgtt gagagagatt caaatgttat aaatgatttg gttccaaatg tgacattaag tttaaaaaaa ccctcaagtg atatggttga ggctaaaatt gaacctgatt atgaggggat taagagggtt cttttagatt ttattggtgc ttataatgag gttcttgctg agattaatat tgtaagctct aatgaagatc agcctaataa tcaaaagtct aatatagttg aagagctaac ttatcttagt gattctcaaa aagaagaggc ttataaaaat ttaggtattc taaggtctga atttttatta aaaaatctta agtccaagct agagtcaata atttttaagc cttatgttac tagtgatcct aatttttcaa taattaatca gatgggagtt tttacaaatt ccatttcttc ttctggtgga ctttctagat atttaagact tgatgagaaa aagtttgatg aatcaattcg taataatatt gataatgtta gagagctttt tttatatgat cttaatggtg acagagtgta tgataatgga attgctaaaa tgctaggaga ttgtctgtcg cctcttgtgg cttccggagg agttatttat aataaaataa agaattacga cttgaaaatt tttaatcaaa aaaataaagt tgaagattat aaaaagaagt acgaagatag agagagaaaa gtggaaggtg aacttaatac cttggatttt accgttaagc gcatgaaaga tcaagaaaat acattaaagg cttttgattt taatcaaaga aataaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BobuA.17489.a.A1.PS01134
PURIFICATION DATe: 6/25/2011
CONCENTRATION: 20.15mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 100µl
PERCENT IDENTITY: 64
PERCENT COVERAGE: 10
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLASGFFVL ATQNPIEQEG TYNLPESQLD RFLLKVNVHY PSVQDEVRLL ILIK
Validated NT Sequence
catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgttg gcatcaggat tttttgttct tgccactcaa aatccaatag agcaagaggg gacttataat ttgccagaat cccaacttga tagattttta ttaaaagtta atgttcatta tccatcagtg caagatgagg taaggctttt gattttaatc aaagaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLASGFFVP GLESKYNTKE IRESMLKSDK AKIDSSFKKL ESLEQEKSAW QLINRKISTL NSLAKELTSL NSPFNLMSGN SSNSEVLTLS TRYGSKNETH KLIVDQIASA DVFLSSNFDP KKVTIPEGDY IFLVGKKEIN VKSNGNIDLL VKDINNKGKG FLSAKIVKSD KNGNSRFVLQ SLKEGKENKL VIKGEGLSFA KQIGILSELK TNFNPNLSDI VVNQSSSNNK LAFENNGLVL NPLSEVSIEI PEDIEITSRS KIKFEVKYFD TGLEEPDSKI IFNPGGATFK DAKVESEDSV VDLGSDLKTP LEKKYIQMNM VKICSKEGSL ELPLINISNN FEEVEVDVGA LSNLEEINIE NKANNKVIVI SNVEIFDPKN RDGHLPINAK SFAENAKIKF DGVDVERDSN VINDLVPNVT LSLKKPSSDM VEAKIEPDYE GIKRVLLDFI GAYNEVLAEI NIVSSNEDQP NNQKSNIVEE LTYLSDSQKE EAYKNLGILR SEFLLKNLKS KLESIIFKPY VTSDPNFSII NQMGVFTNSI SSSGGLSRYL RLDEKKFDES IRNNIDNVRE LFLYDLNGDR VYDNGIAKML GDCLSPLVAS GGVIYNKIKN YDLKIFNQKN KVEDYKKKYE DRERKVEGEL NTLDFTVKRM KDQENTLKAF DFNQRNK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGCATCA GGATTTTTTG TTCCTGGACT TGAGAGTAAG TACAATACTA AAGAAATTCG TGAATCCATG CTTAAGTCCG ATAAGGCTAA AATTGATTCT TCTTTTAAGA AACTTGAATC TTTAGAGCAA GAGAAAAGTG CCTGGCAGTT AATTAATAGA AAAATCTCTA CTTTAAATTC TCTTGCAAAA GAACTTACAT CACTCAACAG TCCTTTTAAT CTAATGTCAG GAAATTCTAG TAATAGCGAA GTTTTAACTT TGTCTACTAG ATATGGATCT AAGAATGAGA CTCATAAATT AATTGTTGAT CAAATAGCGT CAGCTGATGT GTTTTTGTCT TCAAATTTTG ATCCTAAAAA AGTTACAATC CCAGAGGGAG ATTATATATT TTTAGTTGGC AAGAAAGAAA TTAATGTAAA AAGTAATGGC AACATTGATT TACTTGTGAA GGATATTAAT AACAAGGGAA AGGGCTTTTT ATCTGCAAAA ATAGTGAAAA GTGATAAAAA TGGAAATAGT CGTTTTGTTT TGCAATCCTT AAAGGAGGGC AAAGAAAACA AGCTTGTTAT CAAAGGGGAG GGATTGTCTT TTGCTAAGCA AATTGGAATT TTAAGTGAGC TTAAAACCAA TTTTAATCCT AATCTTTCAG ATATTGTTGT AAATCAATCT AGCAGCAACA ATAAACTTGC TTTTGAGAAC AATGGTCTTG TTTTAAATCC GCTTTCAGAA GTATCAATTG AAATTCCTGA AGATATTGAA ATTACATCTA GGAGTAAGAT TAAATTTGAA GTTAAGTATT TTGATACAGG CTTGGAAGAG CCTGATAGTA AGATTATTTT TAATCCCGGA GGGGCTACAT TTAAGGATGC AAAAGTTGAG AGTGAAGATA GTGTAGTTGA TCTTGGATCT GATTTAAAAA CCCCTTTGGA AAAAAAATAT ATTCAAATGA ATATGGTTAA AATATGTAGC AAGGAAGGTT CTTTGGAGCT TCCTTTAATA AATATTTCAA ATAATTTTGA AGAAGTTGAA GTTGATGTTG GAGCTCTTTC TAATTTGGAA GAAATAAATA TTGAAAATAA AGCAAATAAT AAAGTAATTG TGATTAGCAA TGTTGAAATT TTTGATCCAA AAAATAGAGA TGGTCATTTG CCAATAAATG CTAAAAGTTT TGCTGAAAAT GCAAAAATTA AATTTGATGG AGTAGATGTT GAGAGAGATT CAAATGTTAT AAATGATTTG GTTCCAAATG TGACATTAAG TTTAAAAAAA CCCTCAAGTG ATATGGTTGA GGCTAAAATT GAACCTGATT ATGAGGGGAT TAAGAGGGTT CTTTTAGATT TTATTGGTGC TTATAATGAG GTTCTTGCTG AGATTAATAT TGTAAGCTCT AATGAAGATC AGCCTAATAA TCAAAAGTCT AATATAGTTG AAGAGCTAAC TTATCTTAGT GATTCTCAAA AAGAAGAGGC TTATAAAAAT TTAGGTATTC TAAGGTCTGA ATTTTTATTA AAAAATCTTA AGTCCAAGCT AGAGTCAATA ATTTTTAAGC CTTATGTTAC TAGTGATCCT AATTTTTCAA TAATTAATCA GATGGGAGTT TTTACAAATT CCATTTCTTC TTCTGGTGGA CTTTCTAGAT ATTTAAGACT TGATGAGAAA AAGTTTGATG AATCAATTCG TAATAATATT GATAATGTTA GAGAGCTTTT TTTATATGAT CTTAATGGTG ACAGAGTGTA TGATAATGGA ATTGCTAAAA TGCTAGGAGA TTGTCTGTCG CCTCTTGTGG CTTCCGGAGG AGTTATTTAT AATAAAATAA AGAATTACGA CTTGAAAATT TTTAATCAAA AAAATAAAGT TGAAGATTAT AAAAAGAAGT ACGAAGATAG AGAGAGAAAA GTGGAAGGTG AACTTAATAC CTTGGATTTT ACCGTTAAGC GCATGAAAGA TCAAGAAAAT ACATTAAAGG CTTTTGATTT TAATCAAAGA AATAAATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA