BobuA.17439.a

Flagellar filament outer layer protein (BB_0668)

CENTER ID: BobuA.17439.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17439.a.A2.GE30810 Mature protein( BobuA.17439.a ) 24 341
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1059
RefSeq: NP_212802.1
UniProt: P70856

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRKAKSILF FLLSTVLFAQ ETDGLAEGSK RAEPGELVLD FAELARDPSS TRLDLTNYVD YVYSGASGIV KPEDMVVDLG INNWSVLLTP SARLQAYVKN SVVAPAVVKS ESKRYAGDTI LGVRVLFPSY SQSSAMIMPP FKIPFYSGES GNQFLGKGLI DNIKTMKEIK VSVYSLGYEI DLEVLFEDMN GMEYAYSMGT LKFKGWADLI WSNPNYIPNI SSRIIKDDVP NYPLASSKMR FKAFRVSKSH SSKEQNFIFY VKDLRVLYDK LSVSIDSDID SESVFKVYET SGTESLRKLK AHETFKRVLK LREKISMPEG SFQNFVEKIE SEKPEESSPK N
NT Sequence
ATGAAAAGGA AAGCTAAAAG TATTTTATTT TTTTTATTAT CCACTGTTCT TTTTGCTCAA GAGACTGATG GATTAGCAGA GGGTTCTAAA AGGGCAGAGC CTGGAGAATT AGTCTTAGAT TTTGCCGAGC TTGCAAGAGA TCCAAGTTCA ACTAGACTTG ATCTTACAAA TTATGTTGAT TATGTATATT CGGGCGCTTC TGGTATTGTT AAGCCGGAAG ATATGGTTGT AGATCTTGGG ATAAATAATT GGAGCGTTTT ACTTACTCCT TCTGCAAGGT TGCAGGCTTA CGTTAAAAAT TCAGTTGTTG CGCCCGCTGT TGTTAAGAGT GAGTCAAAAA GGTACGCAGG TGATACTATT TTGGGGGTAA GAGTTTTGTT TCCAAGCTAT TCTCAATCAT CTGCTATGAT TATGCCACCA TTTAAAATTC CTTTTTATTC AGGGGAAAGT GGCAATCAAT TTTTAGGCAA AGGTCTTATT GATAACATTA AAACCATGAA AGAAATTAAG GTATCTGTTT ATAGTTTAGG GTATGAGATA GATCTTGAGG TTTTATTTGA AGATATGAAT GGCATGGAAT ATGCTTATTC TATGGGTACT TTAAAGTTTA AAGGGTGGGC TGATTTAATT TGGTCAAATC CTAACTATAT TCCTAATATA TCATCCAGAA TTATTAAAGA CGATGTTCCA AATTATCCTC TTGCTTCAAG TAAAATGAGA TTTAAGGCTT TTAGAGTTTC AAAGTCACAC AGTTCAAAAG TTAAAAATTT CATCTTTTAT GTTAAAGATT TAAGAGTTCT TTATGATAAG CTAAGTGTTT CAATAGATTC TGATATTGAC AGTGAGTCTG TATTTAAAGT TTATGAGACT AGCGGAACTG AATCCCTTCG TAAATTAAAG GCACACGAAA CTTTTAAAAG AGTTTTAAAG CTTAGAGAAA AAATTTCTAT CGCTGAAGGC TCTTTCCAAA ACTTTGTAGA AAAGATTGAG AGTGAAAAAC CTGAAGAATC ATCTCCGAAA AAT
Details for BobuA.17439.a.A2.GE30810
HARVESTED ON: 11/16/2010
SEQUENCED ON: 11/23/2010
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 98
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMGLAEGS KRAEPGELVL DFAELARDPS STRLDLTNYV DYVYSGASGI VKPEDMVVDL GINNWSVLLT PSARLQAYVK NSVVAPAVVK SESKRYAGDT ILGVRVLFPS YSQSSAMIMP PFKIPFYSGE SGNQFLGKGL IDNIKTMKEI KVSVYSLGYE IDLEVLFEDM NGMEYAYSMG TLKFKGWADL IWSNPNYIPN ISSRIIKDDV PNYPLASSKM RFKAFRVSKS HSSKEQNFIF YVKDLRVLYD KLSVSIDSDI DSESVFKVYE TSGTESLRKL KAHETFKRVL KLREKISMPE GSFQNFVEKI ESEKPEESSP
Validated NT Sequence
tcggagatga ttcttcaggt ttttcactct caatcttttc tacaaagttt tggaaagagc cttcaggcat agaaattttt tctctaagct ttaaaactct tttaaaagtt tcgtgtgcct ttaatttacg aagggattca gttccgctag tctcataaac tttaaataca gactcactgt caatatcaga atctattgaa acactcaact tatcataaag aactcttaaa tctttaacat aaaagatgaa attttgctct tttgaactgt gtgactttga aactctaaaa gccttaaatc tcattttact tgaagcaaga ggataatttg gaacatcgtc tttaataatt ctggatgata tattaggaat atagttagga tttgaccaaa ttaaatcagc ccacccttta aactttaaag tacccataga ataagcatat tccatgccat tcatatcttc aaataaaacc tcaagatcta tctcataccc taaactataa acagatacct taatttcttt catggtttta atgttatcaa taagaccttt gcctaaaaat tgattgccac tttcccctga ataaaaagga attttaaatg gtggcataat catagcagat gattgagaat agcttggaaa caaaactctt acccctaaaa tagtatcacc tgcgtacctt tttgactcac tcttaacaac agcgggcgca acaactgaat ttttaacgta agcctgcaac cttgcagaag gagtaagtaa aacgctccaa ttatttatcc caagatctac aaccatatct tccggcttaa caataccaga agcgcccgaa tatacataat caacataatt tgtaagatca agtctagttg aacttggatc tcttgcaagc tcggcaaaat ctaaaactaa ttctccaggc tctgcccttt tagaaccctc tgctaatccc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatnatggtg atgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGLAEGSKR AEPGELVLDF AELARDPSST RLDLTNYVDY VYSGASGIVK PEDMVVDLGI NNWSVLLTPS ARLQAYVKNS VVAPAVVKSE SKRYAGDTIL GVRVLFPSYS QSSAMIMPPF KIPFYSGESG NQFLGKGLID NIKTMKEIKV SVYSLGYEID LEVLFEDMNG MEYAYSMGTL KFKGWADLIW SNPNYIPNIS SRIIKDDVPN YPLASSKMRF KAFRVSKSHS SKVKNFIFYV KDLRVLYDKL SVSIDSDIDS ESVFKVYETS GTESLRKLKA HETFKRVLKL REKISIAEGS FQNFVEKIES EKPEESSPKN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggattagca gagggttcta aaagggcaga gcctggagaa ttagtcttag attttgccga gcttgcaaga gatccaagtt caactagact tgatcttaca aattatgttg attatgtata ttcgggcgct tctggtattg ttaagccgga agatatggtt gtagatcttg ggataaataa ttggagcgtt ttacttactc cttctgcaag gttgcaggct tacgttaaaa attcagttgt tgcgcccgct gttgttaaga gtgagtcaaa aaggtacgca ggtgatacta ttttgggggt aagagttttg tttccaagct attctcaatc atctgctatg attatgccac catttaaaat tcctttttat tcaggggaaa gtggcaatca atttttaggc aaaggtctta ttgataacat taaaaccatg aaagaaatta aggtatctgt ttatagttta gggtatgaga tagatcttga ggttttattt gaagatatga atggcatgga atatgcttat tctatgggta ctttaaagtt taaagggtgg gctgatttaa tttggtcaaa tcctaactat attcctaata tatcatccag aattattaaa gacgatgttc caaattatcc tcttgcttca agtaaaatga gatttaaggc ttttagagtt tcaaagtcac acagttcaaa agttaaaaat ttcatctttt atgttaaaga tttaagagtt ctttatgata agctaagtgt ttcaatagat tctgatattg acagtgagtc tgtatttaaa gtttatgaga ctagcggaac tgaatccctt cgtaaattaa aggcacacga aacttttaaa agagttttaa agcttagaga aaaaatttct atcgctgaag gctctttcca aaactttgta gaaaagattg agagtgaaaa acctgaagaa tcatctccga aaaattaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa