BobuA.17391.a

RNA polymerase sigma factor rpoD (BB_0712)

CENTER ID: BobuA.17391.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17391.a.A1.GE32508 Full length( BobuA.17391.a ) 1 631
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1103
RefSeq: NP_212846.1
UniProt: P52323

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDLEKKYSK LIEGIITHLG DRKSLSFSEL SNLLPDDILE PEILDCICSV LEDRGIRLVN KISELDLVVS EDGNDEEEEV EIESDRNFMI LDDGFQSDEE DIDIDVKLDD CDEEDISVKD DLGSGYIKGN VLKDSHSEDP IKLYLKEIGK EFLLTGNQEV ELAKQMDSGE SIIENILKNE GLVIENYYNL VNTIYSRMER EEFFKREKDK DKESSPDYYN KKKRIASFYK IPLKPIQDRL ISYVDNKHRV YDLGGDIFEK NLKKERLALK ELLRDIPLYQ EELRIFSDDY IDSANKIKDL QRQQRIILSR LKIEKIRDLR VLGRDLTIAE KKIEIEKSLK LKEDAIKEQI TEAQLAQKEL ERIEMYYEYP TDKIISMSEE IAKGKQMMQH AKDQLIKANL RLVVSIAKKY ANRGLHFFDL VQEGNIGLIK AVEKFEYKRG FKFSTYATWW IRQAITRSIS DQARTIRVPV HMIEQINRLN RETRYLIQVL GKDPTDEELS DRLGWELKKV KTVKSVSREP VSLETPIGEE EDSVLSDFIE DKAIKNPANH TSFVVLQDQI RAILGTLPER EQEVVKMRFG LEDGYSLTLE EVGLHFNVTR ERIRQIESKA LRRLKNPKKT QKLKDYLEDL N
NT Sequence
TTGTCGGATT TGGAAAAGAA ATATTCGAAG CTGATAGAGG GTATTATTAC TCATTTGGGA GATAGAAAAT CTCTTAGTTT TAGTGAATTA TCAAATTTGC TTCCCGATGA TATATTAGAA CCAGAGATTC TTGATTGTAT TTGTTCGGTA CTTGAGGATA GGGGAATAAG GTTGGTTAAT AAAATTTCGG AATTAGATCT GGTTGTCAGT GAAGATGGAA ATGATGAAGA GGAAGAGGTT GAGATTGAAT CTGATAGAAA TTTTATGATT TTAGATGATG GTTTTCAAAG TGATGAGGAA GATATTGACA TTGATGTTAA GCTGGATGAT TGTGATGAAG AAGATATTTC TGTTAAGGAT GATTTAGGTT CAGGGTATAT TAAAGGCAAT GTTTTAAAAG ATAGTCACTC AGAAGATCCA ATCAAGCTTT ATTTAAAGGA AATAGGAAAA GAGTTTTTAT TAACGGGAAA TCAAGAAGTT GAACTTGCAA AGCAAATGGA TTCTGGAGAG AGTATAATTG AGAATATTCT TAAGAATGAG GGACTTGTTA TAGAAAATTA TTATAATCTT GTGAATACTA TTTACTCAAG AATGGAAAGA GAAGAGTTTT TTAAAAGAGA AAAAGATAAG GATAAAGAGA GTAGTCCGGA TTATTATAAT AAAAAAAAAA GAATTGCCTC TTTTTACAAA ATTCCTTTAA AGCCAATTCA AGATCGTTTA ATAAGTTATG TAGATAATAA GCATAGGGTG TATGATCTTG GGGGGGATAT TTTTGAAAAG AATTTAAAAA AGGAAAGATT GGCCCTAAAA GAGCTTTTGA GAGACATTCC TTTGTATCAA GAGGAATTAA GGATTTTCTC AGATGATTAT ATTGACTCTG CTAACAAAAT AAAAGATTTA CAAAGACAAC AAAGAATAAT TCTAAGCAGG CTGAAAATTG AAAAAATAAG AGATTTGAGG GTGCTTGGAA GAGATTTGAC TATTGCTGAA AAAAAAATAG AGATAGAAAA ATCTCTCAAG CTTAAAGAAG ATGCTATTAA AGAGCAGATT ACAGAGGCTC AGCTTGCCCA AAAAGAACTT GAGAGAATTG AGATGTATTA TGAATATCCA ACTGATAAAA TAATAAGCAT GTCAGAAGAG ATTGCTAAAG GGAAGCAAAT GATGCAGCAT GCTAAAGATC AGTTGATTAA GGCTAATTTA AGGCTTGTTG TAAGCATTGC TAAAAAATAT GCAAATAGAG GTCTTCATTT TTTTGATCTT GTTCAAGAAG GCAATATTGG ATTGATTAAG GCTGTTGAAA AGTTCGAATA TAAGAGAGGT TTTAAGTTTT CAACCTATGC TACTTGGTGG ATTAGACAAG CCATAACAAG ATCTATTTCT GATCAAGCTC GCACAATTAG AGTTCCTGTG CACATGATTG AGCAAATAAA TAGGCTTAAT AGAGAAACTA GATATTTAAT TCAAGTTTTA GGCAAAGATC CCACAGATGA AGAGCTTTCA GACAGGCTTG GATGGGAACT TAAAAAGGTT AAAACTGTAA AGAGTGTTTC AAGAGAACCT GTTTCTCTTG AAACACCAAT TGGAGAAGAG GAAGATTCTG TTCTTAGCGA TTTTATTGAG GATAAGGCAA TAAAAAATCC TGCAAATCAC ACATCTTTTG TAGTTTTGCA AGATCAAATA AGAGCAATTC TTGGAACTCT TCCTGAAAGA GAACAAGAAG TTGTAAAAAT GAGATTTGGA CTTGAAGATG GCTATTCTTT AACTCTTGAA GAGGTTGGAC TTCATTTTAA TGTTACAAGA GAAAGAATTA GGCAAATTGA ATCTAAAGCA TTAAGGCGGC TTAAAAATCC CAAAAAAACT CAAAAATTAA AAGATTATCT TGAAGATTTA AAT
Details for BobuA.17391.a.A1.GE32508
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 76kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 38
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLSDLEKKY SKLIEGIITH LGDRKSLSFS ELSNLLPDDI LEPEILDCIC SVLEDRGIRL VNKISELDLV VSEDGNDEXE EVEIESDRNF MILDDGFQSD EEDIDIDVKL DDCDEEDISV KDDLXSGYIK GNVLKDSHSX DPIKLYLKEI GKEFLLTGNQ EVELAKQMDS GXSIIENILK NEGLVIENYY NLVNTIYSRM EREEFFKREK DKDKESSPDY YNKKKELXLF TKFX
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgt cggatttgga aaagaaatat tcgaagctga tagagggtat tattactcat ttgggagata gaaaatctct tagttttagt gaattatcaa atttgcttcc cgatgatata ttagaaccag agattcttga ttgtatttgt tcggtacttg aggatagggg aataaggttg gttaataaaa tttcggaatt agatctggtt gtcagtgaag atggaaatga tgaanaggaa gaggttgaga ttgaatctga tagaaatttt atgattttag atgatggttt tcaaagtgat gaggaagata ttgacattga tgttaagctg gatgattgtg atgaagaaga tatttctgtt aaggatgatt tangttcagg gtatattaaa ggcaatgttt taaaagatag tcactcanaa gatccaatca agctttattt aaaggaaata ggaaaagagt ttttattaac gggaaatcaa gaagttgaac ttgcaaagca aatggattct gganagagta taattgagaa tattcttaag aatgagggac ttgttataga aaattattat aatcttgtga atactattta ctcaagaatg gaaagagaag agttttttaa aagagaaaaa gataaggata aagagagtag tccggattat tataataaaa aaaaagaatt gnctcttttt acnaaattcc nttaaagcca attcaagatc gtttaataag ttatgtagat aataagcata gggngnatga nnntgggggg ganatttttg aaaanaattt aaaaaaggaa agattg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSDLEKKY SKLIEGIITH LGDRKSLSFS ELSNLLPDDI LEPEILDCIC SVLEDRGIRL VNKISELDLV VSEDGNDEEE EVEIESDRNF MILDDGFQSD EEDIDIDVKL DDCDEEDISV KDDLGSGYIK GNVLKDSHSE DPIKLYLKEI GKEFLLTGNQ EVELAKQMDS GESIIENILK NEGLVIENYY NLVNTIYSRM EREEFFKREK DKDKESSPDY YNKKKRIASF YKIPLKPIQD RLISYVDNKH RVYDLGGDIF EKNLKKERLA LKELLRDIPL YQEELRIFSD DYIDSANKIK DLQRQQRIIL SRLKIEKIRD LRVLGRDLTI AEKKIEIEKS LKLKEDAIKE QITEAQLAQK ELERIEMYYE YPTDKIISMS EEIAKGKQMM QHAKDQLIKA NLRLVVSIAK KYANRGLHFF DLVQEGNIGL IKAVEKFEYK RGFKFSTYAT WWIRQAITRS ISDQARTIRV PVHMIEQINR LNRETRYLIQ VLGKDPTDEE LSDRLGWELK KVKTVKSVSR EPVSLETPIG EEEDSVLSDF IEDKAIKNPA NHTSFVVLQD QIRAILGTLP EREQEVVKMR FGLEDGYSLT LEEVGLHFNV TRERIRQIES KALRRLKNPK KTQKLKDYLE DLN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgtcggat ttggaaaaga aatattcgaa gctgatagag ggtattatta ctcatttggg agatagaaaa tctcttagtt ttagtgaatt atcaaatttg cttcccgatg atatattaga accagagatt cttgattgta tttgttcggt acttgaggat aggggaataa ggttggttaa taaaatttcg gaattagatc tggttgtcag tgaagatgga aatgatgaag aggaagaggt tgagattgaa tctgatagaa attttatgat tttagatgat ggttttcaaa gtgatgagga agatattgac attgatgtta agctggatga ttgtgatgaa gaagatattt ctgttaagga tgatttaggt tcagggtata ttaaaggcaa tgttttaaaa gatagtcact cagaagatcc aatcaagctt tatttaaagg aaataggaaa agagttttta ttaacgggaa atcaagaagt tgaacttgca aagcaaatgg attctggaga gagtataatt gagaatattc ttaagaatga gggacttgtt atagaaaatt attataatct tgtgaatact atttactcaa gaatggaaag agaagagttt tttaaaagag aaaaagataa ggataaagag agtagtccgg attattataa taaaaaaaaa agaattgcct ctttttacaa aattccttta aagccaattc aagatcgttt aataagttat gtagataata agcatagggt gtatgatctt gggggggata tttttgaaaa gaatttaaaa aaggaaagat tggccctaaa agagcttttg agagacattc ctttgtatca agaggaatta aggattttct cagatgatta tattgactct gctaacaaaa taaaagattt acaaagacaa caaagaataa ttctaagcag gctgaaaatt gaaaaaataa gagatttgag ggtgcttgga agagatttga ctattgctga aaaaaaaata gagatagaaa aatctctcaa gcttaaagaa gatgctatta aagagcagat tacagaggct cagcttgccc aaaaagaact tgagagaatt gagatgtatt atgaatatcc aactgataaa ataataagca tgtcagaaga gattgctaaa gggaagcaaa tgatgcagca tgctaaagat cagttgatta aggctaattt aaggcttgtt gtaagcattg ctaaaaaata tgcaaataga ggtcttcatt tttttgatct tgttcaagaa ggcaatattg gattgattaa ggctgttgaa aagttcgaat ataagagagg ttttaagttt tcaacctatg ctacttggtg gattagacaa gccataacaa gatctatttc tgatcaagct cgcacaatta gagttcctgt gcacatgatt gagcaaataa ataggcttaa tagagaaact agatatttaa ttcaagtttt aggcaaagat cccacagatg aagagctttc agacaggctt ggatgggaac ttaaaaaggt taaaactgta aagagtgttt caagagaacc tgtttctctt gaaacaccaa ttggagaaga ggaagattct gttcttagcg attttattga ggataaggca ataaaaaatc ctgcaaatca cacatctttt gtagttttgc aagatcaaat aagagcaatt cttggaactc ttcctgaaag agaacaagaa gttgtaaaaa tgagatttgg acttgaagat ggctattctt taactcttga agaggttgga cttcatttta atgttacaag agaaagaatt aggcaaattg aatctaaagc attaaggcgg cttaaaaatc ccaaaaaaac tcaaaaatta aaagattatc ttgaagattt aaattaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa