BobuA.17390.a

Flagellar motor switch protein FliG (BB_0290)

CENTER ID: BobuA.17390.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17390.a.A1.GE32507 Full length( BobuA.17390.a ) 1 344
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17390.a.A1.PS01154 Full length( BobuA.17390.a ) 1 344

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.689
RefSeq: NP_212424.1
UniProt: P52610

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEEKKEKEIL DVSALTGKQK AAILLVSIGS EISSKVFKYL SQEEIESLTF EIAKLETITS ELKDNVLLEF KELMMAQEFI QKGGIDYARE LLEKSLGTQK AVDIINNLGS ALQSRPFEFV RRADPANILN FIQQEHPQTI ALILSYLDPQ KASFILSSLP TEVQTNVARR IALMDRTSPE VVREVERVLE KKLASLSSED YTSAGGVDNV VEIINMADRK TEKFIIESLE EEDPELAEEI KKKMFVFEDI VLLDDRSIQR VLREIDGQEL AKALKSVDIP VQEKIFKNMS KRAASMLKED MEFLGPTRRK DVEESQQKIV SLIRKLEEQG EIVISRGGEE DVLV
NT Sequence
ATGGAAGAAA AAAAAGAAAA GGAGATTCTG GATGTTTCTG CTTTAACAGG CAAGCAAAAG GCCGCTATTT TGTTGGTTTC AATAGGTTCT GAAATCTCTT CTAAAGTGTT TAAGTATCTT TCTCAAGAAG AGATAGAGTC TTTGACATTT GAGATAGCAA AGCTTGAGAC AATTACTTCT GAGCTTAAAG ATAATGTTCT TTTAGAGTTT AAAGAATTAA TGATGGCTCA AGAATTTATT CAAAAGGGTG GAATTGATTA TGCAAGAGAG CTTCTTGAAA AATCTCTTGG GACTCAAAAA GCAGTTGATA TTATTAATAA TTTGGGTTCT GCTTTACAGT CTAGACCCTT TGAATTTGTA AGAAGAGCAG ATCCTGCGAA TATTTTAAAC TTTATTCAGC AAGAACATCC CCAAACAATT GCTTTAATTC TTTCATATCT TGATCCTCAA AAGGCTTCTT TTATTCTCTC TAGTTTGCCC ACAGAAGTAC AAACCAATGT TGCAAGAAGA ATTGCGTTAA TGGATAGAAC TTCTCCTGAG GTTGTAAGAG AGGTTGAGAG GGTTCTTGAG AAAAAACTAG CTTCTCTTTC TTCAGAAGAT TATACATCAG CAGGAGGAGT TGACAATGTT GTTGAGATAA TTAATATGGC TGATAGAAAA ACGGAGAAGT TTATTATTGA ATCTCTTGAA GAAGAAGATC CAGAGCTTGC AGAAGAGATT AAGAAGAAAA TGTTTGTGTT TGAGGATATA GTTTTACTTG ATGACAGATC TATACAAAGG GTTTTAAGAG AAATAGATGG TCAAGAGTTG GCAAAAGCTT TAAAATCTGT AGATATTCCT GTCCAAGAAA AAATTTTCAA AAACATGTCA AAAAGAGCGG CTTCAATGCT TAAGGAAGAC ATGGAATTTT TGGGACCTAC TAGGCGAAAA GATGTTGAGG AATCTCAGCA AAAAATTGTT TCTCTTATTA GAAAATTAGA AGAACAGGGA GAAATAGTTA TTTCAAGGGG TGGTGAAGAA GATGTGCTTG TC
Details for BobuA.17390.a.A1.GE32507
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEEKKEKEI LDVSALTGKQ KAAILLVSIG SEISSKVFKY LSQEEIESLT FEIAKLETIT SELKDNVLLE FKELMMAQEF IQKGGIDYAR ELLEKSLGTQ KAVDIINNLG SALQSRPFEF VRRADPANIL NFIQQEHPQT IALILSYLDP QKASFILSSL PTEVQTNVAR RIALMDRTSP EVVREVERVL EKKLASLSSE DYTSAGGVDN VVEIINMADR KTEKFIIESL EEEDPELAEE IKKKMFVFED IVLLDDRSIQ RVLREIDGQE LAKALKSVDI PVQEKIFKNM SKRAASMLKE DMEFLGPTRR KDVEESQQKI VSLIRKLEEQ GEIVISRGGE EDVLV
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggaaga aaaaaaagaa aaggagattc tggatgtttc tgctttaaca ggcaagcaaa aggccgctat tttgttggtt tcaataggtt ctgaaatctc ttctaaagtg tttaagtatc tttctcaaga agagatagag tctttgacat ttgagatagc aaagcttgag acaattactt ctgagcttaa agataatgtt cttttagagt ttaaagaatt aatgatggct caagaattta ttcaaaaggg tggaattgat tatgcaagag agcttcttga aaaatctctt gggactcaaa aagcagttga tattattaat aatttgggtt ctgctttaca gtctagaccc tttgaatttg taagaagagc agatcctgcg aatattttaa actttattca gcaagaacat ccccaaacaa ttgctttaat tctttcatat cttgatcctc aaaaggcttc ttttattctc tctagtttgc ccacagaagt acaaaccaat gttgcaagaa gaattgcgtt aatggataga acttctcctg aggttgtaag agaggttgag agggttcttg agaaaaaact agcttctctt tcttcagaag attatacatc agcaggagga gttgacaatg ttgttgagat aattaatatg gctgatagaa aaacggagaa gtttattatt gaatctcttg aagaagaaga tccagagctt gcagaagaga ttaagaagaa aatgtttgtg tttgaggata tagttttact tgatgacaga tctatacaaa gggttttaag agaaatagat ggtcaagagt tggcaaaagc tttaaaatct gtagatattc ctgtccaaga aaaaattttc aaaaacatgt caaaaagagc ggcttcaatg cttaaggaag acatggaatt tttgggacct actaggcgaa aagatgttga ggaatctcag caaaaaattg tttctcttat tagaaaatta gaagaacagg gagaaatagt tatttcaagg ggtggtgaag aagatgtgct tgtcta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEEKKEKEI LDVSALTGKQ KAAILLVSIG SEISSKVFKY LSQEEIESLT FEIAKLETIT SELKDNVLLE FKELMMAQEF IQKGGIDYAR ELLEKSLGTQ KAVDIINNLG SALQSRPFEF VRRADPANIL NFIQQEHPQT IALILSYLDP QKASFILSSL PTEVQTNVAR RIALMDRTSP EVVREVERVL EKKLASLSSE DYTSAGGVDN VVEIINMADR KTEKFIIESL EEEDPELAEE IKKKMFVFED IVLLDDRSIQ RVLREIDGQE LAKALKSVDI PVQEKIFKNM SKRAASMLKE DMEFLGPTRR KDVEESQQKI VSLIRKLEEQ GEIVISRGGE EDVLV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaagaaaaa aaagaaaagg agattctgga tgtttctgct ttaacaggca agcaaaaggc cgctattttg ttggtttcaa taggttctga aatctcttct aaagtgttta agtatctttc tcaagaagag atagagtctt tgacatttga gatagcaaag cttgagacaa ttacttctga gcttaaagat aatgttcttt tagagtttaa agaattaatg atggctcaag aatttattca aaagggtgga attgattatg caagagagct tcttgaaaaa tctcttggga ctcaaaaagc agttgatatt attaataatt tgggttctgc tttacagtct agaccctttg aatttgtaag aagagcagat cctgcgaata ttttaaactt tattcagcaa gaacatcccc aaacaattgc tttaattctt tcatatcttg atcctcaaaa ggcttctttt attctctcta gtttgcccac agaagtacaa accaatgttg caagaagaat tgcgttaatg gatagaactt ctcctgaggt tgtaagagag gttgagaggg ttcttgagaa aaaactagct tctctttctt cagaagatta tacatcagca ggaggagttg acaatgttgt tgagataatt aatatggctg atagaaaaac ggagaagttt attattgaat ctcttgaaga agaagatcca gagcttgcag aagagattaa gaagaaaatg tttgtgtttg aggatatagt tttacttgat gacagatcta tacaaagggt tttaagagaa atagatggtc aagagttggc aaaagcttta aaatctgtag atattcctgt ccaagaaaaa attttcaaaa acatgtcaaa aagagcggct tcaatgctta aggaagacat ggaatttttg ggacctacta ggcgaaaaga tgttgaggaa tctcagcaaa aaattgtttc tcttattaga aaattagaag aacagggaga aatagttatt tcaaggggtg gtgaagaaga tgtgcttgtc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BobuA.17390.a.A1.PS01154
PURIFICATION DATe: 7/14/2011
CONCENTRATION: 57.22mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEEKKEKEI LDVSALTGKQ KAAILLVSIG SEISSKVFKY LSQEEIESLT FEIAKLETIT SELKDNVLLE FKELMMAQEF IQKGGIDYAR ELLEKSLGTQ KAVDIINNLG SALQSRPFEF VRRADPANIL NFIQQEHPQT IALILSYLDP QKASFILSSL PTEVQTNVAR RIALMDRTSP EVVREVERVL EKKLASLSSE DYTSAGGVDN VVEIINMADR KTEKFIIESL EEEDPELAEE IKKKMFVFED IVLLDDRSIQ RVLREIDGQE LAKALKSVDI PVQEKIFKNM SKRAASMLKE DMEFLGPTRR KDVEESQQKI VSLIRKLEEQ GEIVISRGGE EDVLV
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggaaga aaaaaaagaa aaggagattc tggatgtttc tgctttaaca ggcaagcaaa aggccgctat tttgttggtt tcaataggtt ctgaaatctc ttctaaagtg tttaagtatc tttctcaaga agagatagag tctttgacat ttgagatagc aaagcttgag acaattactt ctgagcttaa agataatgtt cttttagagt ttaaagaatt aatgatggct caagaattta ttcaaaaggg tggaattgat tatgcaagag agcttcttga aaaatctctt gggactcaaa aagcagttga tattattaat aatttgggtt ctgctttaca gtctagaccc tttgaatttg taagaagagc agatcctgcg aatattttaa actttattca gcaagaacat ccccaaacaa ttgctttaat tctttcatat cttgatcctc aaaaggcttc ttttattctc tctagtttgc ccacagaagt acaaaccaat gttgcaagaa gaattgcgtt aatggataga acttctcctg aggttgtaag agaggttgag agggttcttg agaaaaaact agcttctctt tcttcagaag attatacatc agcaggagga gttgacaatg ttgttgagat aattaatatg gctgatagaa aaacggagaa gtttattatt gaatctcttg aagaagaaga tccagagctt gcagaagaga ttaagaagaa aatgtttgtg tttgaggata tagttttact tgatgacaga tctatacaaa gggttttaag agaaatagat ggtcaagagt tggcaaaagc tttaaaatct gtagatattc ctgtccaaga aaaaattttc aaaaacatgt caaaaagagc ggcttcaatg cttaaggaag acatggaatt tttgggacct actaggcgaa aagatgttga ggaatctcag caaaaaattg tttctcttat tagaaaatta gaagaacagg gagaaatagt tatttcaagg ggtggtgaag aagatgtgct tgtcta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEEKKEKEI LDVSALTGKQ KAAILLVSIG SEISSKVFKY LSQEEIESLT FEIAKLETIT SELKDNVLLE FKELMMAQEF IQKGGIDYAR ELLEKSLGTQ KAVDIINNLG SALQSRPFEF VRRADPANIL NFIQQEHPQT IALILSYLDP QKASFILSSL PTEVQTNVAR RIALMDRTSP EVVREVERVL EKKLASLSSE DYTSAGGVDN VVEIINMADR KTEKFIIESL EEEDPELAEE IKKKMFVFED IVLLDDRSIQ RVLREIDGQE LAKALKSVDI PVQEKIFKNM SKRAASMLKE DMEFLGPTRR KDVEESQQKI VSLIRKLEEQ GEIVISRGGE EDVLV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAAGAAAAA AAAGAAAAGG AGATTCTGGA TGTTTCTGCT TTAACAGGCA AGCAAAAGGC CGCTATTTTG TTGGTTTCAA TAGGTTCTGA AATCTCTTCT AAAGTGTTTA AGTATCTTTC TCAAGAAGAG ATAGAGTCTT TGACATTTGA GATAGCAAAG CTTGAGACAA TTACTTCTGA GCTTAAAGAT AATGTTCTTT TAGAGTTTAA AGAATTAATG ATGGCTCAAG AATTTATTCA AAAGGGTGGA ATTGATTATG CAAGAGAGCT TCTTGAAAAA TCTCTTGGGA CTCAAAAAGC AGTTGATATT ATTAATAATT TGGGTTCTGC TTTACAGTCT AGACCCTTTG AATTTGTAAG AAGAGCAGAT CCTGCGAATA TTTTAAACTT TATTCAGCAA GAACATCCCC AAACAATTGC TTTAATTCTT TCATATCTTG ATCCTCAAAA GGCTTCTTTT ATTCTCTCTA GTTTGCCCAC AGAAGTACAA ACCAATGTTG CAAGAAGAAT TGCGTTAATG GATAGAACTT CTCCTGAGGT TGTAAGAGAG GTTGAGAGGG TTCTTGAGAA AAAACTAGCT TCTCTTTCTT CAGAAGATTA TACATCAGCA GGAGGAGTTG ACAATGTTGT TGAGATAATT AATATGGCTG ATAGAAAAAC GGAGAAGTTT ATTATTGAAT CTCTTGAAGA AGAAGATCCA GAGCTTGCAG AAGAGATTAA GAAGAAAATG TTTGTGTTTG AGGATATAGT TTTACTTGAT GACAGATCTA TACAAAGGGT TTTAAGAGAA ATAGATGGTC AAGAGTTGGC AAAAGCTTTA AAATCTGTAG ATATTCCTGT CCAAGAAAAA ATTTTCAAAA ACATGTCAAA AAGAGCGGCT TCAATGCTTA AGGAAGACAT GGAATTTTTG GGACCTACTA GGCGAAAAGA TGTTGAGGAA TCTCAGCAAA AAATTGTTTC TCTTATTAGA AAATTAGAAG AACAGGGAGA AATAGTTATT TCAAGGGGTG GTGAAGAAGA TGTGCTTGTC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA