BobuA.17327.a
Glycine betaine, L-proline ABC transporter, glycine/betaine/L-proline-binding protein (ProX) (BB_0144)
| CENTER ID: | BobuA.17327.a |
| ORGANISM: | Borrelia burgdorferi B31 |
| ASSOCIATED DISEASE: | Lyme disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.17327.a.A2.GE30801 | Mature protein( BobuA.17327.a ) | 16 | 290 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.17327.a.A2.PW30689 | Mature protein( BobuA.17327.a ) | 16 | 290 | ||
| BobuA.17327.a.A2.PW31644 | Mature protein( BobuA.17327.a ) | 16 | 290 |
Structures
3TMG
DEPOSITED: 8/31/2011
DETERMINATION: XRay
CLONE: BobuA.17327.a.A2.GE30801
PROTEIN: BobuA.17327.a.A2.PW31644
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_153473 |
| PATRIC ID: | fig|224326.49.peg.543 |
| RefSeq: | NP_212278.1 |
| UniProt: | O51169 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYKLFLFFII FMFLSCDEKK SSKNLKSVKI GYVNWGGETA ATNVLKVVFE KMGYNAEIFS VTTSIMYQYL ASGKIDGTVS SWVPTADKFY YEKLKTKFVD LGANYEGTIQ GFVVPSYVPI SSISELKGKG DKFKNKMIGI DAGAGTQIVT EQALNYYGLS KEYELVPSSE SVMLASLDSS IKRNEWILVP LWKPHWAFSR YDIKFLDDPD LIMGGIESVH TLVRLGLEND DFDAYYVFDH FYWSDDLILP LMDKNDKEPG KEYRNAVEFV EKNKEIVKTW VPEKYKTLFD
NT Sequence
ATGTATAAAT TATTTTTATT TTTTATTATT TTTATGTTTT TGTCTTGTGA TGAAAAAAAG AGTTCAAAGA ATTTAAAATC GGTAAAAATT GGATATGTGA ATTGGGGTGG AGAAACGGCA GCTACAAATG TATTAAAGGT TGTTTTTGAG AAAATGGGCT ACAATGCAGA AATATTTTCA GTTACTACGT CTATAATGTA TCAATACTTA GCATCTGGAA AGATAGACGG TACGGTGTCT TCTTGGGTTC CTACAGCCGA TAAATTTTAT TATGAAAAAC TGAAAACAAA GTTTGTTGAT CTTGGTGCAA ATTATGAAGG AACCATTCAA GGTTTTGTGG TGCCAAGCTA TGTTCCAATT TCCAGCATTA GTGAGCTTAA GGGTAAAGGT GATAAGTTTA AAAACAAAAT GATTGGCATA GATGCTGGTG CGGGAACTCA AATTGTTACA GAACAAGCGC TTAATTATTA TGGATTAAGT AAAGAGTATG AGCTAGTTCC TTCAAGTGAG AGTGTTATGC TTGCAAGTTT AGATTCTTCA ATAAAGAGAA ACGAATGGAT TTTAGTTCCT TTGTGGAAGC CTCATTGGGC TTTTTCTAGG TATGATATTA AGTTTCTTGA TGATCCTGAT TTAATTATGG GGGGAATTGA GAGCGTGCAT ACTCTTGTTA GACTTGGTCT TGAAAATGAT GATTTTGATG CATATTATGT TTTTGATCAT TTTTATTGGA GCGATGATTT AATATTGCCC TTAATGGATA AAAATGATAA AGAGCCAGGC AAAGAATACC GCAATGCGGT TGAATTTGTT GAAAAGAATA AAGAGATTGT AAAGACGTGG GTTCCAGAAA AATATAAGAC CTTATTTGAT
Details for BobuA.17327.a.A2.GE30801
| HARVESTED ON: | 11/16/2010 |
| SEQUENCED ON: | 11/23/2010 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMCDEKKSSK NLKSVKIGYV NWGGETAATN VLKVVFEKMG YNAEIFSVTT SIMYQYLASG KIDGTVSSWV PTADKFYYEK LKTKFVDLGA NYEGTIQGFV VPSYVPISSI SELKGKGDKF KNKMIGIDAG AGTQIVTEQA LNYYGLSKEY ELVPSSESVM LASLDSSIKR NEWILVPLWK PHWAFSRYDI KFLDDPDLIM GGIESVHTLV RLGLENDDFD AYYVFDHFYW SDDLILPLMD KNDKEPGKEY RNAVEFVEKN KEIVKTWVPE KYKTLFD
Validated NT Sequence
ttattaatca aataaggtct tatatttttc tggaacccac gtctttacaa tctctttatt cttttcaaca aattcaaccg cattgcggta ttctttgcct ggctctttat catttttatc cattaagggc aatattaaat catcgctcca ataaaaatga tcaaaaacat aatatgcatc aaaatcatca ttttcaagac caagtctaac aagagtatgc acgctctcaa ttccccccat aattaaatca ggatcatcaa gaaacttaat atcataccta gaaaaagccc aatgaggctt ccacaaagga actaaaatcc attcgtttct ctttattgaa gaatctaaac ttgcaagcat aacactctca cttgaaggaa ctagctcata ctctttactt aatccataat aattaagcgc ttgttctgta acaatttgag ttcccgcacc agcatctatg ccaatcattt tgtttttaaa cttatcacct ttacccttaa gctcactaat gctggaaatt ggaacatagc ttggcaccac aaaaccttga atggttcctt cataatttgc accaagatca acaaactttg ttttcagttt ttcataataa aatttatcgg ctgtaggaac ccaagaagac accgtaccgt ctatctttcc agatgctaag tattgataca ttatagacgt agtaactgaa aatatttctg cattgtagcc cattttctca aaaacaacct ttaatacatt tgtagctgcc gtttctccac cccaattcac atatccaatt tttaccgatt ttaaattctt tgaactcttt ttttcatcac acatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccatggt atatctcctt cttaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCDEKKSSK NLKSVKIGYV NWGGETAATN VLKVVFEKMG YNAEIFSVTT SIMYQYLASG KIDGTVSSWV PTADKFYYEK LKTKFVDLGA NYEGTIQGFV VPSYVPISSI SELKGKGDKF KNKMIGIDAG AGTQIVTEQA LNYYGLSKEY ELVPSSESVM LASLDSSIKR NEWILVPLWK PHWAFSRYDI KFLDDPDLIM GGIESVHTLV RLGLENDDFD AYYVFDHFYW SDDLILPLMD KNDKEPGKEY RNAVEFVEKN KEIVKTWVPE KYKTLFD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgtgatgaa aaaaagagtt caaagaattt aaaatcggta aaaattggat atgtgaattg gggtggagaa acggcagcta caaatgtatt aaaggttgtt tttgagaaaa tgggctacaa tgcagaaata ttttcagtta ctacgtctat aatgtatcaa tacttagcat ctggaaagat agacggtacg gtgtcttctt gggttcctac agccgataaa ttttattatg aaaaactgaa aacaaagttt gttgatcttg gtgcaaatta tgaaggaacc attcaaggtt ttgtggtgcc aagctatgtt ccaatttcca gcattagtga gcttaagggt aaaggtgata agtttaaaaa caaaatgatt ggcatagatg ctggtgcggg aactcaaatt gttacagaac aagcgcttaa ttattatgga ttaagtaaag agtatgagct agttccttca agtgagagtg ttatgcttgc aagtttagat tcttcaataa agagaaacga atggatttta gttcctttgt ggaagcctca ttgggctttt tctaggtatg atattaagtt tcttgatgat cctgatttaa ttatgggggg aattgagagc gtgcatactc ttgttagact tggtcttgaa aatgatgatt ttgatgcata ttatgttttt gatcattttt attggagcga tgatttaata ttgcccttaa tggataaaaa tgataaagag ccaggcaaag aataccgcaa tgcggttgaa tttgttgaaa agaataaaga gattgtaaag acgtgggttc cagaaaaata taagacctta tttgattaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BobuA.17327.a.A2.PW30689
| PURIFICATION DATe: | 1/24/2011 |
| CONCENTRATION: | 26.53mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMCDEKKSSK NLKSVKIGYV NWGGETAATN VLKVVFEKMG YNAEIFSVTT SIMYQYLASG KIDGTVSSWV PTADKFYYEK LKTKFVDLGA NYEGTIQGFV VPSYVPISSI SELKGKGDKF KNKMIGIDAG AGTQIVTEQA LNYYGLSKEY ELVPSSESVM LASLDSSIKR NEWILVPLWK PHWAFSRYDI KFLDDPDLIM GGIESVHTLV RLGLENDDFD AYYVFDHFYW SDDLILPLMD KNDKEPGKEY RNAVEFVEKN KEIVKTWVPE KYKTLFD
Validated NT Sequence
ttattaatca aataaggtct tatatttttc tggaacccac gtctttacaa tctctttatt cttttcaaca aattcaaccg cattgcggta ttctttgcct ggctctttat catttttatc cattaagggc aatattaaat catcgctcca ataaaaatga tcaaaaacat aatatgcatc aaaatcatca ttttcaagac caagtctaac aagagtatgc acgctctcaa ttccccccat aattaaatca ggatcatcaa gaaacttaat atcataccta gaaaaagccc aatgaggctt ccacaaagga actaaaatcc attcgtttct ctttattgaa gaatctaaac ttgcaagcat aacactctca cttgaaggaa ctagctcata ctctttactt aatccataat aattaagcgc ttgttctgta acaatttgag ttcccgcacc agcatctatg ccaatcattt tgtttttaaa cttatcacct ttacccttaa gctcactaat gctggaaatt ggaacatagc ttggcaccac aaaaccttga atggttcctt cataatttgc accaagatca acaaactttg ttttcagttt ttcataataa aatttatcgg ctgtaggaac ccaagaagac accgtaccgt ctatctttcc agatgctaag tattgataca ttatagacgt agtaactgaa aatatttctg cattgtagcc cattttctca aaaacaacct ttaatacatt tgtagctgcc gtttctccac cccaattcac atatccaatt tttaccgatt ttaaattctt tgaactcttt ttttcatcac acatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccatggt atatctcctt cttaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCDEKKSSK NLKSVKIGYV NWGGETAATN VLKVVFEKMG YNAEIFSVTT SIMYQYLASG KIDGTVSSWV PTADKFYYEK LKTKFVDLGA NYEGTIQGFV VPSYVPISSI SELKGKGDKF KNKMIGIDAG AGTQIVTEQA LNYYGLSKEY ELVPSSESVM LASLDSSIKR NEWILVPLWK PHWAFSRYDI KFLDDPDLIM GGIESVHTLV RLGLENDDFD AYYVFDHFYW SDDLILPLMD KNDKEPGKEY RNAVEFVEKN KEIVKTWVPE KYKTLFD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGTGATGAA AAAAAGAGTT CAAAGAATTT AAAATCGGTA AAAATTGGAT ATGTGAATTG GGGTGGAGAA ACGGCAGCTA CAAATGTATT AAAGGTTGTT TTTGAGAAAA TGGGCTACAA TGCAGAAATA TTTTCAGTTA CTACGTCTAT AATGTATCAA TACTTAGCAT CTGGAAAGAT AGACGGTACG GTGTCTTCTT GGGTTCCTAC AGCCGATAAA TTTTATTATG AAAAACTGAA AACAAAGTTT GTTGATCTTG GTGCAAATTA TGAAGGAACC ATTCAAGGTT TTGTGGTGCC AAGCTATGTT CCAATTTCCA GCATTAGTGA GCTTAAGGGT AAAGGTGATA AGTTTAAAAA CAAAATGATT GGCATAGATG CTGGTGCGGG AACTCAAATT GTTACAGAAC AAGCGCTTAA TTATTATGGA TTAAGTAAAG AGTATGAGCT AGTTCCTTCA AGTGAGAGTG TTATGCTTGC AAGTTTAGAT TCTTCAATAA AGAGAAACGA ATGGATTTTA GTTCCTTTGT GGAAGCCTCA TTGGGCTTTT TCTAGGTATG ATATTAAGTT TCTTGATGAT CCTGATTTAA TTATGGGGGG AATTGAGAGC GTGCATACTC TTGTTAGACT TGGTCTTGAA AATGATGATT TTGATGCATA TTATGTTTTT GATCATTTTT ATTGGAGCGA TGATTTAATA TTGCCCTTAA TGGATAAAAA TGATAAAGAG CCAGGCAAAG AATACCGCAA TGCGGTTGAA TTTGTTGAAA AGAATAAAGA GATTGTAAAG ACGTGGGTTC CAGAAAAATA TAAGACCTTA TTTGATTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for BobuA.17327.a.A2.PW31644
| PURIFICATION DATe: | 1/24/2011 |
| CONCENTRATION: | 26.14mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMCDEKKSSK NLKSVKIGYV NWGGETAATN VLKVVFEKMG YNAEIFSVTT SIMYQYLASG KIDGTVSSWV PTADKFYYEK LKTKFVDLGA NYEGTIQGFV VPSYVPISSI SELKGKGDKF KNKMIGIDAG AGTQIVTEQA LNYYGLSKEY ELVPSSESVM LASLDSSIKR NEWILVPLWK PHWAFSRYDI KFLDDPDLIM GGIESVHTLV RLGLENDDFD AYYVFDHFYW SDDLILPLMD KNDKEPGKEY RNAVEFVEKN KEIVKTWVPE KYKTLFD
Validated NT Sequence
ttattaatca aataaggtct tatatttttc tggaacccac gtctttacaa tctctttatt cttttcaaca aattcaaccg cattgcggta ttctttgcct ggctctttat catttttatc cattaagggc aatattaaat catcgctcca ataaaaatga tcaaaaacat aatatgcatc aaaatcatca ttttcaagac caagtctaac aagagtatgc acgctctcaa ttccccccat aattaaatca ggatcatcaa gaaacttaat atcataccta gaaaaagccc aatgaggctt ccacaaagga actaaaatcc attcgtttct ctttattgaa gaatctaaac ttgcaagcat aacactctca cttgaaggaa ctagctcata ctctttactt aatccataat aattaagcgc ttgttctgta acaatttgag ttcccgcacc agcatctatg ccaatcattt tgtttttaaa cttatcacct ttacccttaa gctcactaat gctggaaatt ggaacatagc ttggcaccac aaaaccttga atggttcctt cataatttgc accaagatca acaaactttg ttttcagttt ttcataataa aatttatcgg ctgtaggaac ccaagaagac accgtaccgt ctatctttcc agatgctaag tattgataca ttatagacgt agtaactgaa aatatttctg cattgtagcc cattttctca aaaacaacct ttaatacatt tgtagctgcc gtttctccac cccaattcac atatccaatt tttaccgatt ttaaattctt tgaactcttt ttttcatcac acatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccatggt atatctcctt cttaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCDEKKSSK NLKSVKIGYV NWGGETAATN VLKVVFEKMG YNAEIFSVTT SIMYQYLASG KIDGTVSSWV PTADKFYYEK LKTKFVDLGA NYEGTIQGFV VPSYVPISSI SELKGKGDKF KNKMIGIDAG AGTQIVTEQA LNYYGLSKEY ELVPSSESVM LASLDSSIKR NEWILVPLWK PHWAFSRYDI KFLDDPDLIM GGIESVHTLV RLGLENDDFD AYYVFDHFYW SDDLILPLMD KNDKEPGKEY RNAVEFVEKN KEIVKTWVPE KYKTLFD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGTGATGAA AAAAAGAGTT CAAAGAATTT AAAATCGGTA AAAATTGGAT ATGTGAATTG GGGTGGAGAA ACGGCAGCTA CAAATGTATT AAAGGTTGTT TTTGAGAAAA TGGGCTACAA TGCAGAAATA TTTTCAGTTA CTACGTCTAT AATGTATCAA TACTTAGCAT CTGGAAAGAT AGACGGTACG GTGTCTTCTT GGGTTCCTAC AGCCGATAAA TTTTATTATG AAAAACTGAA AACAAAGTTT GTTGATCTTG GTGCAAATTA TGAAGGAACC ATTCAAGGTT TTGTGGTGCC AAGCTATGTT CCAATTTCCA GCATTAGTGA GCTTAAGGGT AAAGGTGATA AGTTTAAAAA CAAAATGATT GGCATAGATG CTGGTGCGGG AACTCAAATT GTTACAGAAC AAGCGCTTAA TTATTATGGA TTAAGTAAAG AGTATGAGCT AGTTCCTTCA AGTGAGAGTG TTATGCTTGC AAGTTTAGAT TCTTCAATAA AGAGAAACGA ATGGATTTTA GTTCCTTTGT GGAAGCCTCA TTGGGCTTTT TCTAGGTATG ATATTAAGTT TCTTGATGAT CCTGATTTAA TTATGGGGGG AATTGAGAGC GTGCATACTC TTGTTAGACT TGGTCTTGAA AATGATGATT TTGATGCATA TTATGTTTTT GATCATTTTT ATTGGAGCGA TGATTTAATA TTGCCCTTAA TGGATAAAAA TGATAAAGAG CCAGGCAAAG AATACCGCAA TGCGGTTGAA TTTGTTGAAA AGAATAAAGA GATTGTAAAG ACGTGGGTTC CAGAAAAATA TAAGACCTTA TTTGATTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA