BobuA.17325.a

Flagellar basal-body rod protein flgG (BB_0774)

CENTER ID: BobuA.17325.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17325.a.A2.GE30799 Mature protein( BobuA.17325.a ) 16 265
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17325.a.A2.PS00999 Mature protein( BobuA.17325.a ) 16 265

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1166
RefSeq: NP_212908.1
UniProt: O51715

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMRALWTAAS GMTAQQYNVD TIANNLSNVN TTGFKKIRAE FEDLIYQTHN RAGTPATENT LRPLGNQVGH GTKIAATQRI FEQGKMQSTN LLTDVAIEGD GFYKILLPDG TYAYTRDGSF KIDSNRELVT SQGYKVLPNI LFPEEYIQNS ITISEEGIVS VKIDTSNEPI ELGQIEISRF INPAGLSAIG SNLFKETAGS GQEIAGIPGS EGMGRLRQGI LEMSNVSIAE EMVTMIVAQR AYEINSKAIQ TSDNMLGIAN NLKRQ
NT Sequence
ATGATGAGAG CATTATGGAC AGCAGCAAGT GGAATGACTG CACAACAATA CAATGTAGAT ACAATTGCCA ATAACCTTTC AAATGTAAAT ACTACAGGAT TTAAAAAAAT AAGAGCAGAA TTTGAGGATC TAATTTATCA AACCCATAAC AGAGCAGGAA CCCCTGCAAC TGAAAATACT TTAAGACCAC TTGGAAATCA AGTTGGTCAC GGAACAAAAA TTGCTGCCAC CCAGAGAATA TTTGAACAAG GAAAAATGCA ATCCACAAAT TTACTCACTG ACGTTGCCAT TGAAGGAGAT GGATTTTACA AAATTCTTCT ACCTGATGGA ACTTATGCAT ATACTAGAGA TGGGTCATTT AAAATCGATT CTAATCGAGA GCTTGTAACA AGCCAAGGAT ACAAAGTATT GCCTAATATA CTCTTCCCAG AAGAATATAT CCAAAACTCA ATTACAATAT CTGAAGAGGG AATAGTATCG GTAAAAATTG ATACCAGCAA CGAACCAATA GAGCTTGGGC AAATTGAAAT ATCAAGATTT ATCAATCCTG CAGGACTAAG TGCCATTGGA AGCAATTTAT TTAAAGAAAC AGCTGGATCA GGCCAAGAAA TAGCAGGAAT ACCAGGAAGT GAAGGCATGG GAAGACTAAG GCAAGGCATA CTTGAAATGT CAAATGTATC TATTGCTGAA GAAATGGTAA CAATGATAGT AGCTCAAAGG GCTTATGAAA TAAACTCAAA AGCTATTCAA ACTTCTGACA ATATGTTAGG AATTGCAAAT AACTTAAAAA GGCAA
Details for BobuA.17325.a.A2.GE30799
HARVESTED ON: 11/16/2010
SEQUENCED ON: 11/23/2010
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQYNVDTIA NNLSNVNTTG FKKIRAEFED LIYQTHNRAG TPATENTLRP LGNQVGHGTK IAATQRIFEQ GKMQSTNLLT DVAIEGDGFY KILLPDGTYA YTRDGSFKID SNRELVTSQG YKVLPNILFP EEYIQNSITI SEEGIVSVKI DTSNEPIELG QIEISRFINP AGLSAIGSNL FKETAGSGQE IAGIPGSEGM GRLRQGILEM SNVSIAEEMV TMIVAQRAYE INSKAIQTSD NMLGIANNLK RQ
Validated NT Sequence
tattgccttt ttaagttatt tgcaattcct aacatattgt cagaagtttg aatagctttt gagtttattt cataagccct ttgagctact atcattgtta ccatttcttc agcaatagat acatttgaca tttcaagtat gccttgcctt agtcttccca tgccttcact tcctggtatt cctgctattt cttggcctga tccagctgtt tctttaaata aattgcttcc aatggcactt agtcctgcag gattgataaa tcttgatatt tcaatttgcc caagctctat tggttcgttg ctggtatcaa tttttaccga tactattccc tcttcagata ttgtaattga gttttggata tattcttctg ggaagagtat attaggcaat actttgtatc cttggcttgt tacaagctct cgattagaat cgattttaaa tgacccatct ctagtatatg cataagttcc atcaggtaga agaattttgt aaaatccatc tccttcaatg gcaacgtcag tgagtaaatt tgtggattgc atttttcctt gttcaaatat tctctgggtg gcagcaattt ttgttccgtg accaacttga tttccaagtg gtcttaaagt attttcagtt gcaggggttc ctgctctgtt atgggtttga taaattagat cctcaaattc tgctcttatt tttttaaatc ctgtagtatt tacatttgaa aggttattgg caattgtatc tacattgtat tgcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQYNVDTIA NNLSNVNTTG FKKIRAEFED LIYQTHNRAG TPATENTLRP LGNQVGHGTK IAATQRIFEQ GKMQSTNLLT DVAIEGDGFY KILLPDGTYA YTRDGSFKID SNRELVTSQG YKVLPNILFP EEYIQNSITI SEEGIVSVKI DTSNEPIELG QIEISRFINP AGLSAIGSNL FKETAGSGQE IAGIPGSEGM GRLRQGILEM SNVSIAEEMV TMIVAQRAYE INSKAIQTSD NMLGIANNLK RQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaatacaat gtagatacaa ttgccaataa cctttcaaat gtaaatacta caggatttaa aaaaataaga gcagaatttg aggatctaat ttatcaaacc cataacagag caggaacccc tgcaactgaa aatactttaa gaccacttgg aaatcaagtt ggtcacggaa caaaaattgc tgccacccag agaatatttg aacaaggaaa aatgcaatcc acaaatttac tcactgacgt tgccattgaa ggagatggat tttacaaaat tcttctacct gatggaactt atgcatatac tagagatggg tcatttaaaa tcgattctaa tcgagagctt gtaacaagcc aaggatacaa agtattgcct aatatactct tcccagaaga atatatccaa aactcaatta caatatctga agagggaata gtatcggtaa aaattgatac cagcaacgaa ccaatagagc ttgggcaaat tgaaatatca agatttatca atcctgcagg actaagtgcc attggaagca atttatttaa agaaacagct ggatcaggcc aagaaatagc aggaatacca ggaagtgaag gcatgggaag actaaggcaa ggcatacttg aaatgtcaaa tgtatctatt gctgaagaaa tggtaacaat gatagtagct caaagggctt atgaaataaa ctcaaaagct attcaaactt ctgacaatat gttaggaatt gcaaataact taaaaaggca ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BobuA.17325.a.A2.PS00999
PURIFICATION DATe: 3/9/2011
CONCENTRATION: 30.18mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQYNVDTIA NNLSNVNTTG FKKIRAEFED LIYQTHNRAG TPATENTLRP LGNQVGHGTK IAATQRIFEQ GKMQSTNLLT DVAIEGDGFY KILLPDGTYA YTRDGSFKID SNRELVTSQG YKVLPNILFP EEYIQNSITI SEEGIVSVKI DTSNEPIELG QIEISRFINP AGLSAIGSNL FKETAGSGQE IAGIPGSEGM GRLRQGILEM SNVSIAEEMV TMIVAQRAYE INSKAIQTSD NMLGIANNLK RQ
Validated NT Sequence
tattgccttt ttaagttatt tgcaattcct aacatattgt cagaagtttg aatagctttt gagtttattt cataagccct ttgagctact atcattgtta ccatttcttc agcaatagat acatttgaca tttcaagtat gccttgcctt agtcttccca tgccttcact tcctggtatt cctgctattt cttggcctga tccagctgtt tctttaaata aattgcttcc aatggcactt agtcctgcag gattgataaa tcttgatatt tcaatttgcc caagctctat tggttcgttg ctggtatcaa tttttaccga tactattccc tcttcagata ttgtaattga gttttggata tattcttctg ggaagagtat attaggcaat actttgtatc cttggcttgt tacaagctct cgattagaat cgattttaaa tgacccatct ctagtatatg cataagttcc atcaggtaga agaattttgt aaaatccatc tccttcaatg gcaacgtcag tgagtaaatt tgtggattgc atttttcctt gttcaaatat tctctgggtg gcagcaattt ttgttccgtg accaacttga tttccaagtg gtcttaaagt attttcagtt gcaggggttc ctgctctgtt atgggtttga taaattagat cctcaaattc tgctcttatt tttttaaatc ctgtagtatt tacatttgaa aggttattgg caattgtatc tacattgtat tgcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQYNVDTIA NNLSNVNTTG FKKIRAEFED LIYQTHNRAG TPATENTLRP LGNQVGHGTK IAATQRIFEQ GKMQSTNLLT DVAIEGDGFY KILLPDGTYA YTRDGSFKID SNRELVTSQG YKVLPNILFP EEYIQNSITI SEEGIVSVKI DTSNEPIELG QIEISRFINP AGLSAIGSNL FKETAGSGQE IAGIPGSEGM GRLRQGILEM SNVSIAEEMV TMIVAQRAYE INSKAIQTSD NMLGIANNLK RQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAATACAAT GTAGATACAA TTGCCAATAA CCTTTCAAAT GTAAATACTA CAGGATTTAA AAAAATAAGA GCAGAATTTG AGGATCTAAT TTATCAAACC CATAACAGAG CAGGAACCCC TGCAACTGAA AATACTTTAA GACCACTTGG AAATCAAGTT GGTCACGGAA CAAAAATTGC TGCCACCCAG AGAATATTTG AACAAGGAAA AATGCAATCC ACAAATTTAC TCACTGACGT TGCCATTGAA GGAGATGGAT TTTACAAAAT TCTTCTACCT GATGGAACTT ATGCATATAC TAGAGATGGG TCATTTAAAA TCGATTCTAA TCGAGAGCTT GTAACAAGCC AAGGATACAA AGTATTGCCT AATATACTCT TCCCAGAAGA ATATATCCAA AACTCAATTA CAATATCTGA AGAGGGAATA GTATCGGTAA AAATTGATAC CAGCAACGAA CCAATAGAGC TTGGGCAAAT TGAAATATCA AGATTTATCA ATCCTGCAGG ACTAAGTGCC ATTGGAAGCA ATTTATTTAA AGAAACAGCT GGATCAGGCC AAGAAATAGC AGGAATACCA GGAAGTGAAG GCATGGGAAG ACTAAGGCAA GGCATACTTG AAATGTCAAA TGTATCTATT GCTGAAGAAA TGGTAACAAT GATAGTAGCT CAAAGGGCTT ATGAAATAAA CTCAAAAGCT ATTCAAACTT CTGACAATAT GTTAGGAATT GCAAATAACT TAAAAAGGCA ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA