BobuA.17324.a

Methanol dehydrogenase regulator (MoxR) (BB_0176)

CENTER ID: BobuA.17324.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17324.a.A1.GE32506 Full length( BobuA.17324.a ) 1 337
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.573
RefSeq: NP_212310.1
UniProt: O87927

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILTRSIMKS GFQIDSEVEN ALHLINKFRR EVATRVLGQK EMIDAILMGL LTDGHVLLEG VPGLAKTLAI QTVSDVLDLE FKRIQFTPDL LPSDLTGNMV YKSATGTFKV RKGPVFSNVI LADEINRAPA KVQSALLEAM GERQVTLGDE THRLPDPFFV LATQNPIEQE GTYNLPESQL DRFLLKVNVH YPSVQDEVRL LKIFSVDGRL ENIKVAKVMN AYSLADIKRT VGRVKVDDKI MLYIVTLISA SRERDKKTYP FAKYIEFGAS PRASLSLLKC ARVNALYEGR IFVLPEDVKA VAYSVLRHRI TPSYEAEVEE MSIDDIIRML LSAVALP
NT Sequence
GTGATTTTGA CTAGGAGTAT TATGAAGAGT GGTTTTCAGA TAGATTCAGA AGTAGAGAAT GCATTACATT TGATAAATAA ATTTAGAAGA GAGGTTGCAA CCAGAGTTCT TGGTCAAAAA GAAATGATAG ATGCTATTTT AATGGGTCTT TTAACAGATG GGCATGTTTT ACTTGAAGGG GTTCCGGGTC TTGCCAAGAC TCTTGCAATT CAAACTGTAT CTGATGTTCT TGATCTTGAA TTTAAGCGCA TACAGTTTAC CCCAGATCTT TTGCCATCTG ACCTTACTGG TAATATGGTT TATAAAAGTG CTACAGGCAC TTTTAAAGTT AGAAAAGGTC CAGTATTTTC AAATGTTATT TTAGCAGATG AAATCAACAG GGCTCCTGCA AAAGTTCAGT CTGCTCTTCT TGAGGCTATG GGAGAAAGAC AAGTAACTCT TGGAGACGAA ACTCATAGGC TTCCAGATCC GTTTTTTGTT CTTGCCACTC AAAATCCAAT AGAGCAAGAG GGGACTTATA ATTTGCCAGA ATCCCAACTT GATAGATTTT TATTAAAAGT TAATGTTCAT TATCCATCAG TGCAAGATGA GGTAAGGCTT TTGAAAATAT TTTCAGTAGA TGGGCGTCTT GAGAATATTA AAGTTGCAAA GGTGATGAAT GCTTATTCTT TGGCTGATAT CAAGAGAACG GTTGGTAGAG TAAAAGTTGA TGACAAGATA ATGCTTTATA TTGTTACTTT AATCTCGGCA TCTCGTGAAA GGGATAAAAA AACTTATCCG TTTGCCAAAT ATATTGAATT TGGAGCATCT CCTAGAGCTT CTCTTAGTTT ATTAAAGTGT GCTCGTGTTA ATGCTCTTTA TGAGGGGAGA ATATTTGTTT TACCAGAAGA TGTCAAAGCT GTTGCTTACA GCGTATTAAG GCATAGAATT ACACCATCTT ATGAGGCAGA GGTAGAGGAA ATGAGCATTG ATGATATTAT TAGAATGCTT CTTTCTGCTG TAGCGCTTCC T
Details for BobuA.17324.a.A1.GE32506
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 40kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 88
PERCENT COVERAGE: 108
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVILTRSIM KSGFQIDSEV ENALHLINKF RREVATRVLG QKEMIDAILM GLLTDGHVLL EGVPGLAKTL AIQTVSDVLD LEFKRIQFTP DLLPSDLTGN MVYKSATGTF KVRKGPVFSN VILADEINRA PAKVQSALLE AMGERQVTLG DETHRLPDPF FVLATQNPIE QEGTYNLPES QLDRFLLKVN VHYPSVQDEV RLLKIFSVDG RLENIKVAKV MNAYSLADIK RTVGRVKVDD KIMLYIVTLI SASRERDKKT YPFAKYIEFG ASPRASLSLL KCARVNALYE GXXICXTRXV XXXAYXVXXI XXXIXXXXXX XXSXXXXIXX XXXXXXXXXX XXXXXXXXXX XXXAXXXXXX XXXXXXXX
Validated NT Sequence
tttgtttanc tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgat tttgactagg agtattatga agagtggttt tcagatagat tcagaagtag agaatgcatt acatttgata aataaattta gaagagaggt tgcaaccaga gttcttggtc aaaaagaaat gatagatgct attttaatgg gtcttttaac agatgggcat gttttacttg aaggggttcc gggtcttgcc aagactcttg caattcaaac tgtatctgat gttcttgatc ttgaatttaa gcgcatacag tttaccccag atcttttgcc atctgacctt actggtaata tggtttataa aagtgctaca ggcactttta aagttagaaa aggtccagta ttttcaaatg ttattttagc agatgaaatc aacagggctc ctgcaaaagt tcagtctgct cttcttgagg ctatgggaga aagacaagta actcttggag acgaaactca taggcttcca gatccgtttt ttgttcttgc cactcaaaat ccaatagagc aagaggggac ttataatttg ccagaatccc aacttgatag atttttatta aaagttaatg ttcattatcc atcagtgcaa gatgaggtaa ggcttttgaa aatattttca gtagatgggc gtcttgagaa tattaaagtt gcaaaggtga tgaatgctta ttctttggct gatatcaaga gaacggttgg tagagtaaaa gttgatgaca agataatgct ttatattgtt actttaatct cggcatctcg tgaaagggat aaaaaaactt atccgtttgc caaatatatt gaatttggag catctcctag agcttctctt agtttattaa agtgtgctcg tgttaatgct ctttatgagg ggagnannat ttgtttnacc aganntgtca annctgntgc ttacngcgta tnnnnnatan nnnacnccat cntntgnnnc annnnngang nanngagcat nnnngnnnnn attannangn tnnntnntgn nnnanngcnn nnnnnnnnaa nnnnnncnan nntnnnncan nnnnnnnnnn nnnnnnnngc tnnnnnnnnn nnnnnntnnn nnnnnnnnnn nnnnnnnnnn nnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVILTRSIM KSGFQIDSEV ENALHLINKF RREVATRVLG QKEMIDAILM GLLTDGHVLL EGVPGLAKTL AIQTVSDVLD LEFKRIQFTP DLLPSDLTGN MVYKSATGTF KVRKGPVFSN VILADEINRA PAKVQSALLE AMGERQVTLG DETHRLPDPF FVLATQNPIE QEGTYNLPES QLDRFLLKVN VHYPSVQDEV RLLKIFSVDG RLENIKVAKV MNAYSLADIK RTVGRVKVDD KIMLYIVTLI SASRERDKKT YPFAKYIEFG ASPRASLSLL KCARVNALYE GRIFVLPEDV KAVAYSVLRH RITPSYEAEV EEMSIDDIIR MLLSAVALP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgattttg actaggagta ttatgaagag tggttttcag atagattcag aagtagagaa tgcattacat ttgataaata aatttagaag agaggttgca accagagttc ttggtcaaaa agaaatgata gatgctattt taatgggtct tttaacagat gggcatgttt tacttgaagg ggttccgggt cttgccaaga ctcttgcaat tcaaactgta tctgatgttc ttgatcttga atttaagcgc atacagttta ccccagatct tttgccatct gaccttactg gtaatatggt ttataaaagt gctacaggca cttttaaagt tagaaaaggt ccagtatttt caaatgttat tttagcagat gaaatcaaca gggctcctgc aaaagttcag tctgctcttc ttgaggctat gggagaaaga caagtaactc ttggagacga aactcatagg cttccagatc cgttttttgt tcttgccact caaaatccaa tagagcaaga ggggacttat aatttgccag aatcccaact tgatagattt ttattaaaag ttaatgttca ttatccatca gtgcaagatg aggtaaggct tttgaaaata ttttcagtag atgggcgtct tgagaatatt aaagttgcaa aggtgatgaa tgcttattct ttggctgata tcaagagaac ggttggtaga gtaaaagttg atgacaagat aatgctttat attgttactt taatctcggc atctcgtgaa agggataaaa aaacttatcc gtttgccaaa tatattgaat ttggagcatc tcctagagct tctcttagtt tattaaagtg tgctcgtgtt aatgctcttt atgaggggag aatatttgtt ttaccagaag atgtcaaagc tgttgcttac agcgtattaa ggcatagaat tacaccatct tatgaggcag aggtagagga aatgagcatt gatgatatta ttagaatgct tctttctgct gtagcgcttc cttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa