SSGCID: Target Page

BobuA.17321.a

cell division protein (ftsJ) (BB_0313)

CENTER ID:	BobuA.17321.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.17321.a.B1.GE39131	full length		1	189

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.711
RefSeq:	NP_212447.2
UniProt:	O51293

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDDEYSKKAK REGYLARSVY KLIEINEKFS LFSSGNVLDI GASPGSFSQY AYKKLKRGIL VSVDINDIGL RYDDNFYFIK GDIFLDDTVF KINTFKPYSL VISDVAPKTT GNRLVDTSNS FNLSMRIIDL SLEVLLKKGN LLVKVFQGGD EMQIFKKFEK YFKFVKKIRP KAVRKNSFEI YFLGKSFGK

NT Sequence

ttggatgatg aatattctaa aaaagccaaa agagaaggat atttggcaag gtctgtatat aagttgatag aaattaatga aaaattttct ttattttctt ctggcaatgt tttagatatt ggcgcatcac ctggcagctt ttctcagtat gcttataaaa agcttaaaag aggaattcta gtatctgttg atattaatga tattggcctt agatatgatg ataattttta ttttataaag ggagatatct ttttagatga tacagttttt aaaattaata cgtttaaacc ttatagtctt gtaattagtg atgtggctcc caagactact ggaaatagac ttgtagatac cagcaattct tttaatttaa gcatgagaat aatagattta tcacttgaag ttttacttaa aaaagggaat ttacttgtta aagtttttca gggaggagac gagatgcaaa tttttaaaaa gtttgaaaaa tattttaaat ttgtaaaaaa aattagaccc aaagctgtaa ggaaaaattc ttttgaaatt tattttttag gcaaaagttt tggcaagtag

Details for BobuA.17321.a.B1.GE39131

HARVESTED ON:	5/6/2015
SEQUENCED ON:	5/6/2015
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML DDEYSKKAKR EGYLARSVYK LIEISEKFSL FSSGNVLDIG ASPGSFSQYA YKKLKRGILV SVDINDIGLR YDDNFYFIKG DIFLDDTVFK INTFKPYSLV ISDVAPKTTG NRLVDTSNSF NLSMRIIDLS LEVLLKKGNL LVKVFQGGDE MQIFKKFEKY YKFVKKIRPK AVRKNSFEIY FLGKSFGK

Validated NT Sequence

atcttactca cttacttgcc aaaacttttg cctaaaaaat aaatttcaaa agaatttttc cttacagctt tgggtctaat tttttttaca aatttataat atttttcaaa ctttttaaaa atttgcatct cgtctcctcc ctgaaaaact ttaacaagta aattcccttt tttaagtaaa acttcaagtg ataaatctat tattctcatg cttaaattaa aagaattgct ggtatctaca agtctatttc cagtagtctt gggagccaca tcactaatta caagactata aggtttaaac gtattaattt taaaaactgt atcatctaaa aagatatctc cctttataaa ataaaaatta tcatcatatc taaggccaat atcattaata tcaacagata ctagaattcc tcttttaagc tttttataag catactgaga aaagctgcca ggtgatgcgc caatatctaa aacattgcca gaagaaaata aagaaaattt ttcactaatt tctatcaact tatatacaga ccttgccaaa tatccttctc ttttggcttt tttagaatat tcatcatcca acatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHMD DEYSKKAKRE GYLARSVYKL IEINEKFSLF SSGNVLDIGA SPGSFSQYAY KKLKRGILVS VDINDIGLRY DDNFYFIKGD IFLDDTVFKI NTFKPYSLVI SDVAPKTTGN RLVDTSNSFN LSMRIIDLSL EVLLKKGNLL VKVFQGGDEM QIFKKFEKYF KFVKKIRPKA VRKNSFEIYF LGKSFGK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggatg atgaatattc taaaaaagcc aaaagagaag gatatttggc aaggtctgta tataagttga tagaaattaa tgaaaaattt tctttatttt cttctggcaa tgttttagat attggcgcat cacctggcag cttttctcag tatgcttata aaaagcttaa aagaggaatt ctagtatctg ttgatattaa tgatattggc cttagatatg atgataattt ttattttata aagggagata tctttttaga tgatacagtt tttaaaatta atacgtttaa accttatagt cttgtaatta gtgatgtggc tcccaagact actggaaata gacttgtaga taccagcaat tcttttaatt taagcatgag aataatagat ttatcacttg aagttttact taaaaaaggg aatttacttg ttaaagtttt tcagggagga gacgagatgc aaatttttaa aaagtttgaa aaatatttta aatttgtaaa aaaaattaga cccaaagctg taaggaaaaa ttcttttgaa atttattttt taggcaaaag ttttggcaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat