BobuA.17317.a

Flagellar hook protein flgE (BB_0283)

CENTER ID: BobuA.17317.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17317.a.A1.GE32503 Full length( BobuA.17317.a ) 1 442
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.682
RefSeq: NP_212417.1
UniProt: Q44767

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMRSLYSGVS GLQNHQTRMD VVGNNIANVN TIGFKKGRVN FQDMISQSIS GASRPTDARG GTNPKQVGLG MNVASIDTIH TQGAFQSTQK ASDLGVSGNG FFILKEGKNL FYTRAGAFDV DSDRHLVNPA NGMRIQGWMA RDLEGEKVIN TASDIEDLII PIGDKEGAKS TKNVTFACNL DKRLPLIQEG ANPADIARGT WVVNKSLYDS FGNVSVLELR VVKDLNTPNL WNATVLINGE QNSNFTLGFD NEGALASLNG QPGQKGDILQ IPITFNVLGA NVGEVGEQQT VNLKLGTVGS YTDSITQFAD SSSTKAIIQD GYGMGYMENY EIDQNGVIVG IYSNGIRRDL GKIALASFMN PGGLAKSGDT NFVETSNSGQ VRIGETGLAG LGDIRSGVLE MANVDLAEQF TDMIVTQRGF QANAKTITTS DQLLQELVRL KN
NT Sequence
ATGATGAGGT CTTTATATTC TGGTGTTTCT GGGCTTCAGA ATCATCAAAC AATAATGGAT GTTGTTGGTA ACAATATCGC CAATGTAAAT ACAATTGGCT TTAAAAAGGG AAGAGTAAAT TTTCAAGATA TGATATCGCA ATCTATTTCT GGAGCTTCTC GCCCTACTGA TGCTCGTGGT GGGACTAATC CCAAGCAAGT TGGATTAGGC ATGAATGTTG CCTCAATTGA CACTATTCAC ACTCAAGGAG CTTTTCAAAG CACTCAAAAA GCATCTGATC TTGGGGTTAG TGGCAACGGA TTTTTTATTT TAAAAGAAGG TAAAAATTTG TTTTATACAA GAGCCGGTGC TTTTTACGTG GACTCTGATC GACATCTTGT AAATCCTGCA AATGGAATGC GAATTCAAGG TTGGATGGCA AGAGATTTAG AAGGTGAAAA GGTTATAAAT ACAGCTTCTG ATATTGAGGA TCTGATTATT CCGATTGGAG ATAAAGAGGG AGCAAAGTCT ACTAAAAATA TTACTTTTGC TTGTAATCTT GATAAGAGAT TGCCCTTAAT TCAAGAAGGT GCGAGTCCTG CAGATATTGC ACGCGGAACT TGGGTTGTCA ATAAATCATT GTATGACAGT TTTGGAAATG TTAGTGTTCT TGAGCTTAGA GTTGTAAAAG ATTTAAATAC GCCTAATTTA TGGAATGCAA CAGTATTAAT AAATGGTGAG CAAAATTCAA ATTTTACACT TGGGTTTGAC AATGAAGGAG CATTGGCGTC TTTAAATGGT CAACCAGGGC AAAAAGGAGA TATTCTTCAA ATTCCTATAA CATTTAATGT TTTGGGTGCA AATGTAGGTG AAGTTGGTGA GCAGCAAACT GTAAATTTGA AATTGGGAAC AGTTGGAAGT TACACTGATT CAATTACTCA GTTTGCTGAT TCTAGTAGCA CAAAGGCTAT TATTCAAGAT GGATATGGCA TGGGATATAT GGAAAATTAT GAAATTGATC AAAATGGTGT TATAGTTGGC ATTTATTCAA ATGGCATAAG ACGAGATCTT GGCAAGATTG CTCTTGCTTC TTTTATGAAT CCCGGAGGAC TTGCAAAATC AGGCGATACT AATTTTGTAG AAACAAGCAA TTCGGGTCAA GTTAGAATAG GCGAAACTGG ACTTGCTGGA CTTGGTGATA TTAGATCTGG TGTTTTAGAA ATGGCCAATG TTGATCTTGC AGAGCAATTT ACAGATATGA TAGTGACCCA AAGAGGATTT CAGGCAAATG CCAAAACCAT TACCACTTCT GATCAATTAT TACAAGAACT TGTAAGATTG AAAAAT
Details for BobuA.17317.a.A1.GE32503
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 49kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMMRSLYSGV SGLQNHQTRM DVVGNNIANV NTIGFKKGRV NFQDMISQSI SGASRPTDAR GGTNPKQVGL GMNVASIDTI HTQGAFQSTQ KASDLGVSGN GFFILKEGKN LFYTRAGAFD VDSDRHLVNP ANGMRIQGWM ARDLEGEKVI NTASDIEDLI IPIGDKEGAK STKNVTFACN LDKRLPLIQE GANPADIARG TWVVNKSLYD SFGNVSVLEL RVVKDLNTPN LWNATVLING EQNSNFTLGF DNEGALASLN GQPGQKGDIL QIPITFNVLG ANVGEVGEQQ TVNLKLGTVG SYTDSITQFA DSSSTKAIIQ DGYGMGYMEN YEIDQNGVIV GIYSNGIRRD LGKIALASFM NPGGLAKSGD TNFVETSNSG QVRIGETGLA GLGDIRSGVL EMANVDLAEQ FTDMIVTQRG FQANAKTITT SDQLLQELVR L
Validated NT Sequence
gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg atgaggtctt tatattctgg tgtttctggg cttcagaatc atcaaacaag aatggatgtt gttggtaaca atatcgccaa tgtaaataca attggcttta aaaagggaag agtaaatttt caagatatga tatcgcaatc tatttctgga gcttctcgcc ctactgatgc tcgtggtggg actaatccca agcaagttgg attaggcatg aatgttgcct caattgacac tattcacact caaggagctt ttcaaagcac tcaaaaagca tctgatcttg gagttagtgg caacggattt tttattttaa aagaaggtaa aaatttgttt tatacaagag ccggtgcttt tgacgtggac tctgatcgac atcttgtaaa tcctgcaaat ggaatgcgaa ttcaaggttg gatggcaaga gatttagaag gtgaaaaggt tataaataca gcttctgata ttgaggatct gattattccg attggagata aagagggagc aaagtctacg aaaaatgtta cttttgcttg taatcttgat aagagattgc ccttaattca agaaggtgcg aatcctgcag atattgcacg cggaacttgg gttgtcaata aatcattgta tgacagtttt ggaaatgtta gtgttcttga gcttagagtt gtaaaagatt taaatacgcc taatttatgg aatgcaacag tattaataaa tggtgagcaa aattcaaatt ttacacttgg gtttgacaat gaaggagcat tggcgtcttt aaatggtcaa ccagggcaaa aaggagatat tcttcaaatt cctataacat ttaatgtttt gggtgcaaat gtaggtgaag ttggtgagca gcaaactgta aatttgaaat tgggaacagt tggaagttac actgattcaa ttactcagtt tgctgattct agtagcacaa aggctattat tcaagatgga tatggcatgg gatatatgga aaattatgaa attgatcaaa atggtgttat agttggcatt tattcaaatg gcataagacg agatcttggc aagattgctc ttgcttcttt tatgaatccc ggaggacttg caaaatcagg cgatactaat tttgtagaaa caagcaattc aggtcaagtt agaataggcg aaactggact tgctggactt ggtgatatta gatctggtgt tttagaaatg gccaatgttg atcttgcaga gcaatttaca gatatgatag tgacccaaag aggatttcag gcaaatgcca aaaccattac cacttctgat caattattac aagaacttgt aagattga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMRSLYSGV SGLQNHQTIM DVVGNNIANV NTIGFKKGRV NFQDMISQSI SGASRPTDAR GGTNPKQVGL GMNVASIDTI HTQGAFQSTQ KASDLGVSGN GFFILKEGKN LFYTRAGAFY VDSDRHLVNP ANGMRIQGWM ARDLEGEKVI NTASDIEDLI IPIGDKEGAK STKNITFACN LDKRLPLIQE GASPADIARG TWVVNKSLYD SFGNVSVLEL RVVKDLNTPN LWNATVLING EQNSNFTLGF DNEGALASLN GQPGQKGDIL QIPITFNVLG ANVGEVGEQQ TVNLKLGTVG SYTDSITQFA DSSSTKAIIQ DGYGMGYMEN YEIDQNGVIV GIYSNGIRRD LGKIALASFM NPGGLAKSGD TNFVETSNSG QVRIGETGLA GLGDIRSGVL EMANVDLAEQ FTDMIVTQRG FQANAKTITT SDQLLQELVR LKN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatgaggtct ttatattctg gtgtttctgg gcttcagaat catcaaacaa taatggatgt tgttggtaac aatatcgcca atgtaaatac aattggcttt aaaaagggaa gagtaaattt tcaagatatg atatcgcaat ctatttctgg agcttctcgc cctactgatg ctcgtggtgg gactaatccc aagcaagttg gattaggcat gaatgttgcc tcaattgaca ctattcacac tcaaggagct tttcaaagca ctcaaaaagc atctgatctt ggggttagtg gcaacggatt ttttatttta aaagaaggta aaaatttgtt ttatacaaga gccggtgctt tttacgtgga ctctgatcga catcttgtaa atcctgcaaa tggaatgcga attcaaggtt ggatggcaag agatttagaa ggtgaaaagg ttataaatac agcttctgat attgaggatc tgattattcc gattggagat aaagagggag caaagtctac taaaaatatt acttttgctt gtaatcttga taagagattg cccttaattc aagaaggtgc gagtcctgca gatattgcac gcggaacttg ggttgtcaat aaatcattgt atgacagttt tggaaatgtt agtgttcttg agcttagagt tgtaaaagat ttaaatacgc ctaatttatg gaatgcaaca gtattaataa atggtgagca aaattcaaat tttacacttg ggtttgacaa tgaaggagca ttggcgtctt taaatggtca accagggcaa aaaggagata ttcttcaaat tcctataaca tttaatgttt tgggtgcaaa tgtaggtgaa gttggtgagc agcaaactgt aaatttgaaa ttgggaacag ttggaagtta cactgattca attactcagt ttgctgattc tagtagcaca aaggctatta ttcaagatgg atatggcatg ggatatatgg aaaattatga aattgatcaa aatggtgtta tagttggcat ttattcaaat ggcataagac gagatcttgg caagattgct cttgcttctt ttatgaatcc cggaggactt gcaaaatcag gcgatactaa ttttgtagaa acaagcaatt cgggtcaagt tagaataggc gaaactggac ttgctggact tggtgatatt agatctggtg ttttagaaat ggccaatgtt gatcttgcag agcaatttac agatatgata gtgacccaaa gaggatttca ggcaaatgcc aaaaccatta ccacttctga tcaattatta caagaacttg taagattgaa aaattaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa