SSGCID: Target Page

BobuA.17284.c

Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (OppA-1) (BB_0328)

CENTER ID:	BobuA.17284.c
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.17284.c.A2.GE32501	Mature protein( BobuA.17284.c )		28	529

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.17284.c.A2.PS01133	Mature protein( BobuA.17284.c )		28	529

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.725
RefSeq:	NP_212462.1
UniProt:	O51307

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKENPMKYI KIALMLIIFS LIACISNAKK EKIVFRVSNL SEPSSLDPQL STDLYGSNII TNLFLGLAVK DSQTGKYKPG LAKSWNISED GIIYTFNLRE DIVWSDGVAI TAEEIKKSYL RILNKKTAAM YANLIKSTIK NAQEYFDETV PESELGIKAI DSKTLEITLT SPKPYFPDML THSAYIPVPM HIVEKYGENW TNPENIVVSG AYKLKERSIN DKIVIEKNEK YYNAKNVEID EVIFYPTEGS VAYNMYINGE LDFLQGAEKN NLEEIKIRDD YYSGLKNGMA YIAFNTTIKP LDNLKVRQAI SLAIDRETLT KVVLKGSSDP TRNLTPKFDD YSYGKNLILF DPENAKKLLA EAGYPDGKGF PTLKYKISEG RPTTAEFLQE QFKKILNINL EIENEEWTTF LGSRRTGNYQ MSSVGWIGDY FDPLTFLDSL FTTENHFLGA YKYSNKEYDA LIKKSNFELD PIKRQDILRQ AEEIIAEKDF PMAPLYIPKS HYLFRNDKWT GWVPNIAESY LYEDIKTKK

NT Sequence

TTGAAAAAGG AAAATCCCAT GAAATATATA AAAATAGCCT TAATGCTAAT AATTTTTTCT TTAATAGCAT GTATTAGTAA TGCTAAAAAA GAAAAAATAG TTTTCAGAGT ATCAAACTTA AGCGAGCCAT CATCACTTGA TCCTCAACTC TCAACAGACC TTTACGGTAG CAACATTATT ACAAACCTAT TCTTAGGCCT AGCGGTAAAA GATTCTCAAA CTGGAAAATA TAAACCAGGA CTTGCAAAAA GTTGGAATAT TTCTGAAGAT GGAATTATTT ACACATTTAA CCTAAGAGAA GATATAGTTT GGAGCGATGG AGTTGCCATT ACTGCCGAGG AGATAAAAAA ATCATACCTA AGAATTTTAA ATAAAAAAAC AGCTGCAATG TATGCTAATT TAATAAAATC TACAATAAAA AATGCACAAG AATATTTCGA TGAGACAGTG CCTGAATCTG AGCTTGGCAT AAAGGCTATT GACAGCAAAA CCTTAGAGAT AACATTAACA TCTCCAAAGC CTTATTTTCC TGATATGCTA ACACACTCAG CATACATACC AGTTCCAATG CATATTGTTG AAAAATATGG AGAAAATTGG ACAAATCCTG AAAATATAGT TGTTAGTGGC GCATACAAAC TTAAAGAAAG ATCAATTAAC GATAAAATCG TAATAGAAAA AAATGAAAAA TACTATAATG CAAAAAATGT AGAAATTGAT GAAGTAATAT TTTACCCAAC AGAAGGTAGC GTGGCTTACA ATATGTACAT AAACGGTGAA CTCGATTTTC TACAAGGAGC AGAAAAGAAT AATTTAGAAG AAATTAAAAT AAGAGATGAT TATTATTCTG GGTTAAAAAA CGGAATGGCA TACATAGCAT TCAATACAAC AATAAAACCA CTAGACAATT TAAAAGTTAG ACAAGCCATC TCCCTTGCCA TTGACAGAGA AACTTTAACT AAAGTAGTTT TAAAGGGAAG TTCAGATCCA ACAAGAAATC TAACTCCAAA ATTTGATGAT TATTCTTATG GAAAAAATTT AATACTATTT GATCCTGAGA ATGCAAAAAA ACTTTTAGCT GAAGCTGGAT ATCCGGATGG GAAAGGATTC CCCACATTAA AATATAAAAT ATCGGAGGGA AGACCAACAA CAGCAGAATT TTTGCAAGAA CAATTTAAAA AAATACTAAA CATTAACTTA GAAATCGAGA ATGAAGAATG GACAACATTC CTAGGAAGCA GAAGAACTGG AAATTACCAA ATGTCAAGCG TGGGGTGGAT AGGAGATTAT TTTGATCCCT TAACATTCTT AGACAGCTTA TTTACAACAG AAAATCATTT TTTAGGAGCG TACAAATATT CAAACAAAGA GTATGATGCT TTAATAAAAA AATCTAATTT TGAACTTGAT CCAATAAAAA GACAAGACAT TTTAAGACAA GCTGAAGAGA TAATAGCAGA AAAAGACTTT CCTATGGCAC CTTTATATAT ACCCAAATCT CATTATCTTT TCAGAAATGA TAAATGGACA GGGTGGGTAC CAAATATCGC AGAAAGCTAT TTATATGAAG ATATTAAAAC TAAAAAA

Details for BobuA.17284.c.A2.GE32501

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	59kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAKKEKIVF RVSNLSEPSS LDPQLSTDLY GSNIITNLFL GLAVKDSQTG KYKPGLAKSW NISEDGIIYT FNLREDIVWS DGVAITAEEI KKSYLRILNK KTAAMYANLI KSTIKNAQEY FDETVPESEL GIKAIDSKTL EITLTSPKPY FPDMLTHSAY IPVPMHIVEK YGENWTNPEN IVVSXAYKLK ERSINDKIVI EKNEKYYNAK NVEIDEVIFY PTEGSVAYNM YINGELDFLQ GAEKNNLEEI KIRDDYYSGL KNGMAYIAFN TTIKPLDNLK VRQAISLAID RETLTKVVLK GSSDPTRNLT PKFDDYSYGK NLILFDPENA KKLLAEAGYP DGKGFPTLKY KISEGRPTTA EFLQEQFKKI LNINLEIENE EWTTFLGSRR TGNYQMSSVG WIGDYFDPLT FLDSLFTTEN HFLGAYKYSN KEYDALIKKS NFELDPIKRQ DILRQAEEII AEKDFPMAPL YIPKSHYLFR NDKWTGWVPN IAESYLYEDI KT

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcta aaaaagaaaa aatagttttc agagtatcaa acttaagcga gccatcatca cttgatcctc aactctcaac agacctttac ggtagcaaca ttattacaaa cctattctta ggcctagcgg taaaagattc tcaaactgga aaatataaac caggacttgc aaaaagttgg aatatttctg aagatggaat tatttacaca tttaacctaa gagaagatat agtttggagc gatggagttg ccattactgc cgaggagata aaaaaatcat acctaagaat tttaaataaa aaaacagctg caatgtatgc taatttaata aaatctacaa taaaaaatgc acaagaatat ttcgatgaga cagtgcctga atctgagctt ggcataaagg ctattgacag caaaacctta gagataacat taacatctcc aaagccttat tttcctgata tgctaacaca ctcagcatac ataccagttc caatgcatat tgttgaaaaa tatggagaaa attggacaaa tcctgaaaat atagttgtta gtgncgcata caaacttaaa gaaagatcaa ttaacgataa aatcgtaata gaaaaaaatg aaaaatacta taatgcaaaa aatgtagaaa ttgatgaagt aatattttac ccaacagaag gtagcgtggc ttacaatatg tacataaacg gtgaactcga ttttctacaa ggagcagaaa agaataattt agaagaaatt aaaataagag atgattatta ttctgggtta aaaaacggaa tggcatacat agcattcaat acaacaataa aaccactaga caatttaaaa gttagacaag ccatctccct tgccattgac agagaaactt taactaaagt agttttaaag ggaagttcag atccaacaag aaatctaact ccaaaatttg atgattattc ttatggaaaa aatttaatac tatttgatcc tgagaatgca aaaaaacttt tagctgaagc tggatatccg gatgggaaag gattccccac attaaaatat aaaatatcgg agggaagacc aacaacagca gaatttttgc aagaacaatt taaaaaaata ctaaacatta acttagaaat cgagaatgaa gaatggacaa cattcctagg aagcagaaga actggaaatt accaaatgtc aagcgtgggg tggataggag attattttga tcccttaaca ttcttagaca gcttatttac aacagaaaat cattttttag gagcgtacaa atattcaaac aaagagtatg atgctttaat aaaaaaatct aattttgaac ttgatccaat aaaaagacaa gacattttaa gacaagctga agagataata gcagaaaaag actttcctat ggcaccttta tatataccca aatctcatta tcttttcaga aatgataaat ggacagggtg ggtaccaaat atcgcagaaa gctatttata tgaagatatt aaaacta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAKKEKIVF RVSNLSEPSS LDPQLSTDLY GSNIITNLFL GLAVKDSQTG KYKPGLAKSW NISEDGIIYT FNLREDIVWS DGVAITAEEI KKSYLRILNK KTAAMYANLI KSTIKNAQEY FDETVPESEL GIKAIDSKTL EITLTSPKPY FPDMLTHSAY IPVPMHIVEK YGENWTNPEN IVVSGAYKLK ERSINDKIVI EKNEKYYNAK NVEIDEVIFY PTEGSVAYNM YINGELDFLQ GAEKNNLEEI KIRDDYYSGL KNGMAYIAFN TTIKPLDNLK VRQAISLAID RETLTKVVLK GSSDPTRNLT PKFDDYSYGK NLILFDPENA KKLLAEAGYP DGKGFPTLKY KISEGRPTTA EFLQEQFKKI LNINLEIENE EWTTFLGSRR TGNYQMSSVG WIGDYFDPLT FLDSLFTTEN HFLGAYKYSN KEYDALIKKS NFELDPIKRQ DILRQAEEII AEKDFPMAPL YIPKSHYLFR NDKWTGWVPN IAESYLYEDI KTKK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctaaaaaa gaaaaaatag ttttcagagt atcaaactta agcgagccat catcacttga tcctcaactc tcaacagacc tttacggtag caacattatt acaaacctat tcttaggcct agcggtaaaa gattctcaaa ctggaaaata taaaccagga cttgcaaaaa gttggaatat ttctgaagat ggaattattt acacatttaa cctaagagaa gatatagttt ggagcgatgg agttgccatt actgccgagg agataaaaaa atcataccta agaattttaa ataaaaaaac agctgcaatg tatgctaatt taataaaatc tacaataaaa aatgcacaag aatatttcga tgagacagtg cctgaatctg agcttggcat aaaggctatt gacagcaaaa ccttagagat aacattaaca tctccaaagc cttattttcc tgatatgcta acacactcag catacatacc agttccaatg catattgttg aaaaatatgg agaaaattgg acaaatcctg aaaatatagt tgttagtggc gcatacaaac ttaaagaaag atcaattaac gataaaatcg taatagaaaa aaatgaaaaa tactataatg caaaaaatgt agaaattgat gaagtaatat tttacccaac agaaggtagc gtggcttaca atatgtacat aaacggtgaa ctcgattttc tacaaggagc agaaaagaat aatttagaag aaattaaaat aagagatgat tattattctg ggttaaaaaa cggaatggca tacatagcat tcaatacaac aataaaacca ctagacaatt taaaagttag acaagccatc tcccttgcca ttgacagaga aactttaact aaagtagttt taaagggaag ttcagatcca acaagaaatc taactccaaa atttgatgat tattcttatg gaaaaaattt aatactattt gatcctgaga atgcaaaaaa acttttagct gaagctggat atccggatgg gaaaggattc cccacattaa aatataaaat atcggaggga agaccaacaa cagcagaatt tttgcaagaa caatttaaaa aaatactaaa cattaactta gaaatcgaga atgaagaatg gacaacattc ctaggaagca gaagaactgg aaattaccaa atgtcaagcg tggggtggat aggagattat tttgatccct taacattctt agacagctta tttacaacag aaaatcattt tttaggagcg tacaaatatt caaacaaaga gtatgatgct ttaataaaaa aatctaattt tgaacttgat ccaataaaaa gacaagacat tttaagacaa gctgaagaga taatagcaga aaaagacttt cctatggcac ctttatatat acccaaatct cattatcttt tcagaaatga taaatggaca gggtgggtac caaatatcgc agaaagctat ttatatgaag atattaaaac taaaaaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for BobuA.17284.c.A2.PS01133

PURIFICATION DATe:	6/24/2011
CONCENTRATION:	61.58mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	100µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAKKEKIVF RVSNLSEPSS LDPQLSTDLY GSNIITNLFL GLAVKDSQTG KYKPGLAKSW NISEDGIIYT FNLREDIVWS DGVAITAEEI KKSYLRILNK KTAAMYANLI KSTIKNAQEY FDETVPESEL GIKAIDSKTL EITLTSPKPY FPDMLTHSAY IPVPMHIVEK YGENWTNPEN IVVSGAYKLK ERSINDKIVI EKNEKYYNAK NVEIDEVIFY PTEGSVAYNM YINGELDFLQ GAEKNNLEEI KIRDDYYSGL KNGMAYIAFN TTIKPLDNLK VRQAISLAID RETLTKVVLK GSSDPTRNLT PKFDDYSYGK NLILFDPENA KKLLAEAGYP DGKGFPTLKY KISEGRPTTA EFLQEQFKKI LNINLEIENE EWTTFLGSRR TGNYQMSSVG WIGDYFDPLT FLDSLFTTEN HFLGAYKYSN KEYDALIKKS NFELDPIKRQ DILRQAEEII AEKDFPMAPL YIPKSHYLFR NDKWTGWVPN IAESYLYEDI KTKK

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTAAAAAA GAAAAAATAG TTTTCAGAGT ATCAAACTTA AGCGAGCCAT CATCACTTGA TCCTCAACTC TCAACAGACC TTTACGGTAG CAACATTATT ACAAACCTAT TCTTAGGCCT AGCGGTAAAA GATTCTCAAA CTGGAAAATA TAAACCAGGA CTTGCAAAAA GTTGGAATAT TTCTGAAGAT GGAATTATTT ACACATTTAA CCTAAGAGAA GATATAGTTT GGAGCGATGG AGTTGCCATT ACTGCCGAGG AGATAAAAAA ATCATACCTA AGAATTTTAA ATAAAAAAAC AGCTGCAATG TATGCTAATT TAATAAAATC TACAATAAAA AATGCACAAG AATATTTCGA TGAGACAGTG CCTGAATCTG AGCTTGGCAT AAAGGCTATT GACAGCAAAA CCTTAGAGAT AACATTAACA TCTCCAAAGC CTTATTTTCC TGATATGCTA ACACACTCAG CATACATACC AGTTCCAATG CATATTGTTG AAAAATATGG AGAAAATTGG ACAAATCCTG AAAATATAGT TGTTAGTGGC GCATACAAAC TTAAAGAAAG ATCAATTAAC GATAAAATCG TAATAGAAAA AAATGAAAAA TACTATAATG CAAAAAATGT AGAAATTGAT GAAGTAATAT TTTACCCAAC AGAAGGTAGC GTGGCTTACA ATATGTACAT AAACGGTGAA CTCGATTTTC TACAAGGAGC AGAAAAGAAT AATTTAGAAG AAATTAAAAT AAGAGATGAT TATTATTCTG GGTTAAAAAA CGGAATGGCA TACATAGCAT TCAATACAAC AATAAAACCA CTAGACAATT TAAAAGTTAG ACAAGCCATC TCCCTTGCCA TTGACAGAGA AACTTTAACT AAAGTAGTTT TAAAGGGAAG TTCAGATCCA ACAAGAAATC TAACTCCAAA ATTTGATGAT TATTCTTATG GAAAAAATTT AATACTATTT GATCCTGAGA ATGCAAAAAA ACTTTTAGCT GAAGCTGGAT ATCCGGATGG GAAAGGATTC CCCACATTAA AATATAAAAT ATCGGAGGGA AGACCAACAA CAGCAGAATT TTTGCAAGAA CAATTTAAAA AAATACTAAA CATTAACTTA GAAATCGAGA ATGAAGAATG GACAACATTC CTAGGAAGCA GAAGAACTGG AAATTACCAA ATGTCAAGCG TGGGGTGGAT AGGAGATTAT TTTGATCCCT TAACATTCTT AGACAGCTTA TTTACAACAG AAAATCATTT TTTAGGAGCG TACAAATATT CAAACAAAGA GTATGATGCT TTAATAAAAA AATCTAATTT TGAACTTGAT CCAATAAAAA GACAAGACAT TTTAAGACAA GCTGAAGAGA TAATAGCAGA AAAAGACTTT CCTATGGCAC CTTTATATAT ACCCAAATCT CATTATCTTT TCAGAAATGA TAAATGGACA GGGTGGGTAC CAAATATCGC AGAAAGCTAT TTATATGAAG ATATTAAAAC TAAAAAATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA