BobuA.17284.b

Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (OppAV) (BB_A34)

CENTER ID: BobuA.17284.b
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17284.b.A2.GE32500 Mature protein( BobuA.17284.b ) 23 529
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17284.b.A2.PS01124 Mature protein( BobuA.17284.b ) 23 529

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1549
RefSeq: NP_045707.1
UniProt: O50927

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIIKKRGLLI LGIATVISCS AMSKPKDDIV FGVGIGNEPT SLDPQFCSDR LGNLIINELF VGLLRGDPKT GGYRPGLAKN WDISYDNTCY TFHLRDDIYW SDGIKITAST IRDSYIMALE NELDVPHINL LTSKIKNAQE FYETKLNADE VGVKVINEQT LEITLSRPAR YFLDMLTHPI FIPIPTHIVK KFGNKWTSSE NMVTSGPFKL KRRILNEEIS LEKNEKFYDA KNVAINELIF VTINDTRRIY SMYEHNEIDA IFNNIPLSLI NELILREDFY SADINQIGFF SLNTNVKPLN NVKVREALAL AVDRETLTYR VLDNNFKATR KISPNINNYN YGKKLKPYNP RKAEKLLAEA GYPDGKNFPT LTIKYNKNDL EKEVASFIQS QWKKVLNIDV EIEPETWVNH ISNILKGQYE ISVRSWQGDY LDPMAFFNIF ETKNSHFASY GYSNPELDEL IIKSDLEEDE KLRLEILKKI EEIIIENDYP IIPIYSVAGN YLFKNDKWLG WNTNNSERFA LSEIKPIEE
NT Sequence
ATGATAATAA AAAAAAGAGG ACTTTTAATA CTGGGCATTG CTACTGTAAT CTCGTGCTCA GCAATGTCTA AACCAAAAGA TGATATTGTC TTTGGTGTTG GAATTGGAAA CGAACCAACA TCGCTTGATC CACAATTTTG CAGCGATAGG CTAGGTAATT TAATTATAAA CGAACTATTT GTAGGGCTTT TAAGGGGTGA CCCCAAAACG GGTGGATACA GGCCAGGACT TGCAAAAAAT TGGGACATTT CTTACGACAA CACCTGCTAT ACATTCCATT TAAGAGACGA CATTTATTGG AGTGACGGCA TTAAAATAAC AGCAAGCACC ATTAGAGATT CCTACATAAT GGCCTTAGAA AATGAGCTTG ATGTGCCACA TATTAACCTA TTAACATCAA AGATAAAAAA TGCACAAGAA TTTTACGAAA CAAAATTAAA TGCAGATGAA GTTGGAGTAA AGGTAATCAA TGAGCAAACT TTAGAAATAA CTCTCTCTCG CCCAGCAAGA TATTTTCTTG ACATGCTTAC ACACCCAATA TTTATACCCA TTCCTACGCA CATTGTTAAA AAGTTTGGAA ACAAATGGAC AAGCTCAGAA AACATGGTTA CAAGCGGGCC TTTCAAGCTA AAAAGAAGGA TCTTAAACGA AGAAATATCT CTTGAAAAAA ATGAAAAATT CTATGATGCT AAAAACGTTG CTATTAATGA GCTCATATTT GTTACTATAA ACGACACCCG CAGAATTTAC AGTATGTACG AGCACAACGA AATAGATGCT ATTTTTAATA ACATCCCCTT AAGCCTTATT AATGAGCTTA TCTTAAGAGA AGATTTTTAT TCAGCTGACA TTAATCAAAT TGGGTTTTTT TCACTAAACA CAAATGTTAA ACCTCTTAAC AATGTAAAAG TAAGAGAAGC ATTGGCTCTT GCGGTTGACA GAGAAACTCT AACTTACAGA GTTCTTGACA ACAACTTTAA AGCAACAAGA AAAATAAGCC CTAATATTAA CAACTATAAT TATGGGAAAA AGCTTAAGCC ATACAACCCG CGAAAAGCAG AAAAGCTCTT AGCCGAAGCT GGATATCCAG ATGGTAAAAA TTTTCCAACT CTTACAATAA AATACAATAA AAATGACCTT GAAAAAGAAG TTGCTAGTTT TATTCAAAGC CAATGGAAAA AAGTTTTAAA TATTGATGTA GAAATTGAGC CTGAAACATG GGTCAACCAC ATATCAAATA TTTTAAAAGG TCAATATGAG ATCTCAGTAA GGTCCTGGCA AGGAGATTAT TTAGATCCCA TGGCATTCTT TAATATATTT GAAACTAAAA ACTCACATTT TGCATCTTAC GGATATTCAA ATCCTGAACT TGATGAGCTT ATAATAAAAT CCGATCTTGA AGAGGATGAA AAACTAAGAT TAGAAATATT AAAAAAGATA GAAGAAATAA TTATTGAAAA TGATTATCCA ATAATTCCAA TATACAGTGT TGCGGGAAAC TATCTTTTTA AAAACGACAA ATGGCTAGGG TGGAATACAA ACAATTCAGA AAGATTTGCC CTATCAGAAA TAAAACCTAT AGAAGAA
Details for BobuA.17284.b.A2.GE32500
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 61kDa
OBSERVED MW: 61kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKPKDDIV FGVGIGNEPT SLDPQFCSDR LGNLIINELF VGLLRGDPKT GGYRPGLAKN WDISYDNTCY TFHLRDDIYW SDGIKITAST IRDSYIMALE NELDVPHINL LTSKIKNAQE FYETKLNADE VGVKVINEQT LEITLSRPAR YFLDMLTHPI FIPIPTHIVK KFGNKWTSSE NMVTSGPFKL KRRILNEEIS LEKNEKFYDA KNVAINELIF VTINDTRRIY SMYEHNEIDA IFNNIPLSLI NELILREDFY SADINQIGFF SLNTNVKPLN NVKVREALAL AVDRETLTYR VLDNNFKATR KISPNINNYN YGKKLKPYNP RKAEKLLAEA GYPDGKNFPT LTIKYNKNDL EKEVASFIQS QWKKVLNIDV EIEPETWVNH ISNILKGQYE ISVRSWQGDY LDPMAFFNIF ETKNSHFASY GYSNPELDEL IIKSDLEEDE KLRLEILKKI EEIIIENDYP IIPIYSVAGN YLFKNDKWLG WNTNNSERFA LSEIKPI
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctaaacca aaagatgata ttgtctttgg tgttggaatt ggaaacgaac caacatcgct tgatccacaa ttttgcagcg ataggctagg taatttaatt ataaacgaac tatttgtagg gcttttaagg ggtgacccca aaacgggtgg atacaggcca ggacttgcaa aaaattggga catttcttac gacaacacct gctatacatt ccatttaaga gacgacattt attggagtga cggcattaaa ataacagcaa gcaccattag agattcctac ataatggcct tagaaaatga gcttgatgtg ccacatatta acctattaac atcaaagata aaaaatgcac aagaatttta cgaaacaaaa ttaaatgcag atgaagttgg agtaaaggta atcaatgagc aaactttaga aataactctc tctcgcccag caagatattt tcttgacatg cttacacacc caatatttat acccattcct acgcacattg ttaaaaagtt tggaaacaaa tggacaagct cagaaaacat ggttacaagc gggcctttca agctaaaaag aaggatctta aacgaagaaa tatctcttga aaaaaatgaa aaattctatg atgctaaaaa cgttgctatt aatgagctca tatttgttac tataaacgac acccgcagaa tttacagtat gtacgagcac aacgaaatag atgctatttt taataacatc cccttaagcc ttattaatga gcttatctta agagaagatt tttattcagc tgacattaat caaattgggt ttttttcact aaacacaaat gttaaacctc ttaacaatgt aaaagtaaga gaagcattgg ctcttgcggt tgacagagaa actctaactt acagagttct tgacaacaac tttaaagcaa caagaaaaat aagccctaat attaacaact ataattatgg gaaaaagctt aagccataca acccgcgaaa agcagaaaag ctcttagccg aagctggata tccagatggt aaaaattttc caactcttac aataaaatac aataaaaatg accttgaaaa agaagttgct agttttattc aaagccaatg gaaaaaagtt ttaaatattg atgtagaaat tgagcctgaa acatgggtca accacatatc aaatatttta aaaggtcaat atgagatctc agtaaggtcc tggcaaggag attatttaga tcccatggca ttctttaata tatttgaaac taaaaactca cattttgcat cttacggata ttcaaatcct gaacttgatg agcttataat aaaatccgat cttgaagagg atgaaaaact aagattagaa atattaaaaa agatagaaga aataattatt gaaaatgatt atccaataat tccaatatac agtgttgcgg gaaactatct ttttaaaaac gacaaatggc tagggtggaa tacaaacaat tcagaaagat ttgccctatc agaaataaaa cctatag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKPKDDIV FGVGIGNEPT SLDPQFCSDR LGNLIINELF VGLLRGDPKT GGYRPGLAKN WDISYDNTCY TFHLRDDIYW SDGIKITAST IRDSYIMALE NELDVPHINL LTSKIKNAQE FYETKLNADE VGVKVINEQT LEITLSRPAR YFLDMLTHPI FIPIPTHIVK KFGNKWTSSE NMVTSGPFKL KRRILNEEIS LEKNEKFYDA KNVAINELIF VTINDTRRIY SMYEHNEIDA IFNNIPLSLI NELILREDFY SADINQIGFF SLNTNVKPLN NVKVREALAL AVDRETLTYR VLDNNFKATR KISPNINNYN YGKKLKPYNP RKAEKLLAEA GYPDGKNFPT LTIKYNKNDL EKEVASFIQS QWKKVLNIDV EIEPETWVNH ISNILKGQYE ISVRSWQGDY LDPMAFFNIF ETKNSHFASY GYSNPELDEL IIKSDLEEDE KLRLEILKKI EEIIIENDYP IIPIYSVAGN YLFKNDKWLG WNTNNSERFA LSEIKPIEE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctaaacca aaagatgata ttgtctttgg tgttggaatt ggaaacgaac caacatcgct tgatccacaa ttttgcagcg ataggctagg taatttaatt ataaacgaac tatttgtagg gcttttaagg ggtgacccca aaacgggtgg atacaggcca ggacttgcaa aaaattggga catttcttac gacaacacct gctatacatt ccatttaaga gacgacattt attggagtga cggcattaaa ataacagcaa gcaccattag agattcctac ataatggcct tagaaaatga gcttgatgtg ccacatatta acctattaac atcaaagata aaaaatgcac aagaatttta cgaaacaaaa ttaaatgcag atgaagttgg agtaaaggta atcaatgagc aaactttaga aataactctc tctcgcccag caagatattt tcttgacatg cttacacacc caatatttat acccattcct acgcacattg ttaaaaagtt tggaaacaaa tggacaagct cagaaaacat ggttacaagc gggcctttca agctaaaaag aaggatctta aacgaagaaa tatctcttga aaaaaatgaa aaattctatg atgctaaaaa cgttgctatt aatgagctca tatttgttac tataaacgac acccgcagaa tttacagtat gtacgagcac aacgaaatag atgctatttt taataacatc cccttaagcc ttattaatga gcttatctta agagaagatt tttattcagc tgacattaat caaattgggt ttttttcact aaacacaaat gttaaacctc ttaacaatgt aaaagtaaga gaagcattgg ctcttgcggt tgacagagaa actctaactt acagagttct tgacaacaac tttaaagcaa caagaaaaat aagccctaat attaacaact ataattatgg gaaaaagctt aagccataca acccgcgaaa agcagaaaag ctcttagccg aagctggata tccagatggt aaaaattttc caactcttac aataaaatac aataaaaatg accttgaaaa agaagttgct agttttattc aaagccaatg gaaaaaagtt ttaaatattg atgtagaaat tgagcctgaa acatgggtca accacatatc aaatatttta aaaggtcaat atgagatctc agtaaggtcc tggcaaggag attatttaga tcccatggca ttctttaata tatttgaaac taaaaactca cattttgcat cttacggata ttcaaatcct gaacttgatg agcttataat aaaatccgat cttgaagagg atgaaaaact aagattagaa atattaaaaa agatagaaga aataattatt gaaaatgatt atccaataat tccaatatac agtgttgcgg gaaactatct ttttaaaaac gacaaatggc tagggtggaa tacaaacaat tcagaaagat ttgccctatc agaaataaaa cctatagaag aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BobuA.17284.b.A2.PS01124
PURIFICATION DATe: 6/14/2011
CONCENTRATION: 41.43mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKPKDDIV FGVGIGNEPT SLDPQFCSDR LGNLIINELF VGLLRGDPKT GGYRPGLAKN WDISYDNTCY TFHLRDDIYW SDGIKITAST IRDSYIMALE NELDVPHINL LTSKIKNAQE FYETKLNADE VGVKVINEQT LEITLSRPAR YFLDMLTHPI FIPIPTHIVK KFGNKWTSSE NMVTSGPFKL KRRILNEEIS LEKNEKFYDA KNVAINELIF VTINDTRRIY SMYEHNEIDA IFNNIPLSLI NELILREDFY SADINQIGFF SLNTNVKPLN NVKVREALAL AVDRETLTYR VLDNNFKATR KISPNINNYN YGKKLKPYNP RKAEKLLAEA GYPDGKNFPT LTIKYNKNDL EKEVASFIQS QWKKVLNIDV EIEPETWVNH ISNILKGQYE ISVRSWQGDY LDPMAFFNIF ETKNSHFASY GYSNPELDEL IIKSDLEEDE KLRLEILKKI EEIIIENDYP IIPIYSVAGN YLFKNDKWLG WNTNNSERFA LSEIKPI
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctaaacca aaagatgata ttgtctttgg tgttggaatt ggaaacgaac caacatcgct tgatccacaa ttttgcagcg ataggctagg taatttaatt ataaacgaac tatttgtagg gcttttaagg ggtgacccca aaacgggtgg atacaggcca ggacttgcaa aaaattggga catttcttac gacaacacct gctatacatt ccatttaaga gacgacattt attggagtga cggcattaaa ataacagcaa gcaccattag agattcctac ataatggcct tagaaaatga gcttgatgtg ccacatatta acctattaac atcaaagata aaaaatgcac aagaatttta cgaaacaaaa ttaaatgcag atgaagttgg agtaaaggta atcaatgagc aaactttaga aataactctc tctcgcccag caagatattt tcttgacatg cttacacacc caatatttat acccattcct acgcacattg ttaaaaagtt tggaaacaaa tggacaagct cagaaaacat ggttacaagc gggcctttca agctaaaaag aaggatctta aacgaagaaa tatctcttga aaaaaatgaa aaattctatg atgctaaaaa cgttgctatt aatgagctca tatttgttac tataaacgac acccgcagaa tttacagtat gtacgagcac aacgaaatag atgctatttt taataacatc cccttaagcc ttattaatga gcttatctta agagaagatt tttattcagc tgacattaat caaattgggt ttttttcact aaacacaaat gttaaacctc ttaacaatgt aaaagtaaga gaagcattgg ctcttgcggt tgacagagaa actctaactt acagagttct tgacaacaac tttaaagcaa caagaaaaat aagccctaat attaacaact ataattatgg gaaaaagctt aagccataca acccgcgaaa agcagaaaag ctcttagccg aagctggata tccagatggt aaaaattttc caactcttac aataaaatac aataaaaatg accttgaaaa agaagttgct agttttattc aaagccaatg gaaaaaagtt ttaaatattg atgtagaaat tgagcctgaa acatgggtca accacatatc aaatatttta aaaggtcaat atgagatctc agtaaggtcc tggcaaggag attatttaga tcccatggca ttctttaata tatttgaaac taaaaactca cattttgcat cttacggata ttcaaatcct gaacttgatg agcttataat aaaatccgat cttgaagagg atgaaaaact aagattagaa atattaaaaa agatagaaga aataattatt gaaaatgatt atccaataat tccaatatac agtgttgcgg gaaactatct ttttaaaaac gacaaatggc tagggtggaa tacaaacaat tcagaaagat ttgccctatc agaaataaaa cctatag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKPKDDIV FGVGIGNEPT SLDPQFCSDR LGNLIINELF VGLLRGDPKT GGYRPGLAKN WDISYDNTCY TFHLRDDIYW SDGIKITAST IRDSYIMALE NELDVPHINL LTSKIKNAQE FYETKLNADE VGVKVINEQT LEITLSRPAR YFLDMLTHPI FIPIPTHIVK KFGNKWTSSE NMVTSGPFKL KRRILNEEIS LEKNEKFYDA KNVAINELIF VTINDTRRIY SMYEHNEIDA IFNNIPLSLI NELILREDFY SADINQIGFF SLNTNVKPLN NVKVREALAL AVDRETLTYR VLDNNFKATR KISPNINNYN YGKKLKPYNP RKAEKLLAEA GYPDGKNFPT LTIKYNKNDL EKEVASFIQS QWKKVLNIDV EIEPETWVNH ISNILKGQYE ISVRSWQGDY LDPMAFFNIF ETKNSHFASY GYSNPELDEL IIKSDLEEDE KLRLEILKKI EEIIIENDYP IIPIYSVAGN YLFKNDKWLG WNTNNSERFA LSEIKPIEE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTAAACCA AAAGATGATA TTGTCTTTGG TGTTGGAATT GGAAACGAAC CAACATCGCT TGATCCACAA TTTTGCAGCG ATAGGCTAGG TAATTTAATT ATAAACGAAC TATTTGTAGG GCTTTTAAGG GGTGACCCCA AAACGGGTGG ATACAGGCCA GGACTTGCAA AAAATTGGGA CATTTCTTAC GACAACACCT GCTATACATT CCATTTAAGA GACGACATTT ATTGGAGTGA CGGCATTAAA ATAACAGCAA GCACCATTAG AGATTCCTAC ATAATGGCCT TAGAAAATGA GCTTGATGTG CCACATATTA ACCTATTAAC ATCAAAGATA AAAAATGCAC AAGAATTTTA CGAAACAAAA TTAAATGCAG ATGAAGTTGG AGTAAAGGTA ATCAATGAGC AAACTTTAGA AATAACTCTC TCTCGCCCAG CAAGATATTT TCTTGACATG CTTACACACC CAATATTTAT ACCCATTCCT ACGCACATTG TTAAAAAGTT TGGAAACAAA TGGACAAGCT CAGAAAACAT GGTTACAAGC GGGCCTTTCA AGCTAAAAAG AAGGATCTTA AACGAAGAAA TATCTCTTGA AAAAAATGAA AAATTCTATG ATGCTAAAAA CGTTGCTATT AATGAGCTCA TATTTGTTAC TATAAACGAC ACCCGCAGAA TTTACAGTAT GTACGAGCAC AACGAAATAG ATGCTATTTT TAATAACATC CCCTTAAGCC TTATTAATGA GCTTATCTTA AGAGAAGATT TTTATTCAGC TGACATTAAT CAAATTGGGT TTTTTTCACT AAACACAAAT GTTAAACCTC TTAACAATGT AAAAGTAAGA GAAGCATTGG CTCTTGCGGT TGACAGAGAA ACTCTAACTT ACAGAGTTCT TGACAACAAC TTTAAAGCAA CAAGAAAAAT AAGCCCTAAT ATTAACAACT ATAATTATGG GAAAAAGCTT AAGCCATACA ACCCGCGAAA AGCAGAAAAG CTCTTAGCCG AAGCTGGATA TCCAGATGGT AAAAATTTTC CAACTCTTAC AATAAAATAC AATAAAAATG ACCTTGAAAA AGAAGTTGCT AGTTTTATTC AAAGCCAATG GAAAAAAGTT TTAAATATTG ATGTAGAAAT TGAGCCTGAA ACATGGGTCA ACCACATATC AAATATTTTA AAAGGTCAAT ATGAGATCTC AGTAAGGTCC TGGCAAGGAG ATTATTTAGA TCCCATGGCA TTCTTTAATA TATTTGAAAC TAAAAACTCA CATTTTGCAT CTTACGGATA TTCAAATCCT GAACTTGATG AGCTTATAAT AAAATCCGAT CTTGAAGAGG ATGAAAAACT AAGATTAGAA ATATTAAAAA AGATAGAAGA AATAATTATT GAAAATGATT ATCCAATAAT TCCAATATAC AGTGTTGCGG GAAACTATCT TTTTAAAAAC GACAAATGGC TAGGGTGGAA TACAAACAAT TCAGAAAGAT TTGCCCTATC AGAAATAAAA CCTATAGAAG AATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA