BobuA.17284.a
Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (OppAIV) (BB_B16)
| CENTER ID: | BobuA.17284.a |
| ORGANISM: | Borrelia burgdorferi B31 |
| ASSOCIATED DISEASE: | Lyme disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.17284.a.A2.GE32499 | Mature protein( BobuA.17284.a ) | 24 | 530 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.17284.a.A2.PS01128 | Mature protein( BobuA.17284.a ) | 24 | 530 |
Structures
4GL8
DEPOSITED: 8/13/2012
DETERMINATION: XRay
CLONE: BobuA.17284.a.A2.GE32499
PROTEIN: BobuA.17284.a.A2.PS01128
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131268 |
| PATRIC ID: | fig|224326.49.peg.1604 |
| RefSeq: | NP_047002.1 |
| UniProt: | O31315 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKILIKKLKV VLFLNLILLI SCVNESNRNK LVFKLNIGSE PATLDAQLIN DTVGSGIVSQ MFLGILDGDP RTGGYRPGLA KSWDISDDGV VYTFHLRDNL VWSDGVSITA EGIRKSYLRI LDKETGSSFV NMIKSVIKNA EEYFDGKANE SELGIKALDE KTLEITLKSP KPYFLDMLVH QTFIPVPMHV IEKYGQRWTD PENMVVSGPF KLKSRVLNEK VVLEKNNKYY NSKDVVLDSI IFFVTDNSIT AYNMYLNDEL DAIFKNVPPD LLKDLKLRDD YYSMGINSTS FYSLNMKVKP LDNVKVRKAL SFAIDRKTLT ESVLNDSSIP TRRATPDYID YSYKSNLSLF DAEMAKKLLA DAGYPNGNNF PLLKVKYNTS DSQRKIAEFI QNQWKKNLNI NVQLENEEWS TYINSRVNGN YEIIRSGWSG DYADPMTFLS IFQTENTSFS SYGYSNSEYD ELLIKSDNER DIFKRQEILK KAEAIIIERD FPAVFLNITS SSYLFRNDKW KGWEPNISER FNLSEIKPIK
NT Sequence
ATGAAAATAT TGATAAAAAA GTTAAAAGTT GTATTATTTC TCAATTTAAT TTTACTTATT TCTTGTGTTA ATGAAAGTAA TAGAAACAAA TTGGTTTTTA AGCTAAATAT TGGAAGTGAG CCTGCTACTT TAGATGCTCA ATTAATAAAC GATACGGTTG GATCAGGGAT TGTAAGCCAA ATGTTTCTTG GCATTTTAGA TGGAGATCCC AGGACTGGAG GATACAGACC GGGACTTGCT AAAAGTTGGG ATATTTCTGA TGACGGAGTA GTTTATACGT TTCATTTAAG AGATAATCTT GTTTGGAGTG ATGGAGTTTC CATTACTGCC GAAGGAATAA GAAAATCTTA TCTTAGAATT TTAGATAAAG AAACCGGCTC ATCTTTTGTT AACATGATTA AGTCCGTTAT TAAAAATGCA GAAGAGTATT TTGACGGCAA AGCAAATGAG TCTGAGCTTG GAATTAAAGC TCTTGATGAA AAAACTTTAG AAATAACGCT AAAATCTCCA AAGCCATATT TTCTTGATAT GTTAGTACAT CAAACATTTA TTCCTGTACC AATGCACGTT ATTGAAAAGT ATGGGCAAAG GTGGACAGAC CCCGAGAATA TGGTTGTTAG TGGTCCTTTC AAATTAAAAT CTAGAGTTTT AAATGAAAAG GTTGTTCTTG AAAAGAATAA TAAATATTAT AATTCCAAAG ATGTTGTTCT TGACAGTATT ATATTTTTTG TCACAGATAA TAGCATTACA GCTTATAATA TGTATTTAAA TGATGAGCTG GATGCAATTT TTAAGAATGT TCCACCAGAT TTGCTTAAGG ATCTTAAGCT TAGGGACGAT TATTATTCAA TGGGTATTAA TTCAACCTCT TTTTATTCTT TGAACATGAA AGTAAAACCG CTTGACAATG TTAAAGTTAG AAAGGCGCTG TCTTTTGCTA TTGATAGAAA AACCTTAACA GAGAGCGTTC TTAATGATAG TTCTATTCCT ACAAGAAGAG CAACTCCAGA TTATATTGAT TACTCTTATA AAAGCAATTT GAGCTTATTT GATGCTGAAA TGGCAAAGAA GCTTTTGGCA GATGCAGGAT ATCCCAATGG TAATAATTTT CCTTTATTAA AAGTAAAGTA CAATACAAGT GATAGCCAGA GAAAAATTGC TGAATTTATT CAAAATCAGT GGAAGAAAAA CTTAAATATT AATGTACAGC TTGAGAATGA AGAATGGTCA ACATATATAA ATAGTAGAGT AAATGGTAAT TATGAAATAA TAAGATCAGG ATGGTCAGGA GATTATGCTG ATCCTATGAC GTTCTTAAGC ATCTTTCAAA CTGAAAATAC ATCTTTTTCA TCTTATGGAT ATTCAAATTC TGAATATGAT GAACTTTTAA TAAAATCAGA TAATGAGAGA GATATTTTTA AAAGACAGGA AATTCTAAAA AAAGCAGAAG CAATAATAAT TGAAAGAGAT TTTCCGGCTG TATTTCTTAA TATAACTTCT TCTAGTTATC TTTTTAGAAA CGACAAGTGG AAAGGTTGGG AGCCAAATAT TTCGGAAAGA TTTAATTTAT CTGAAATAAA ACCAATTAAA
Details for BobuA.17284.a.A2.GE32499
| HARVESTED ON: | 5/17/2011 |
| SEQUENCED ON: | 5/23/2011 |
| EXPECTED MW: | 60kDa |
| OBSERVED MW: | 60kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNESNRNKL VFKLNIGSEP ATLDAQLIND TVGSGIVSQM FLGILDGDPR TGGYRPGLAK SWDISDDGVV YTFHLRDNLV WSDGVSITAE GIRKSYLRIL DKETGSSFVN MIKSVIKNAE EYFDGKANES ELGIKALDEK TLEITLKSXK PYFLDMLVHQ TFIPVPMHVI EKYGQRWTDP ENMVVSGPFK LKSRVLNEKV VLEKNNKYYN SKDVVLDSII FFVTDNSITA YNMYLNDELD AIFKNVPPDL LKDLKLRDDY YSMGINSTSF YSLNMKVKPL DNVKVRKALS FAIDRKTLTE SVLNDSSIPT RRATPDYIDY SYKSNLSLFD AEMAKKLLAD AGYPNGNNFP LLKVKYNTSD SQRKIAEFIQ NQWKKNLNIN VQLENEEWST YINSRVNGNY EIIRSGWSGD YADPMTFLSI FQTENTSFSS YGYSNSEYDE LLIKSDNERD IFKRQEILKK AEAIIIERDF PAVFLNITSS SYLFRNDKWK GWEPNISERF NLSEIKPIK
Validated NT Sequence
ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgaat gaaagtaata gaaacaaatt ggtttttaag ctaaatattg gaagtgagcc tgctacttta gatgctcaat taataaacga tacggttgga tcagggattg taagccaaat gtttcttggc attttagatg gagatcccag gactggagga tacagaccgg gacttgctaa aagttgggat atttctgatg acggagtagt ttatacgttt catttaagag ataatcttgt ttggagtgat ggagtttcca ttactgccga aggaataaga aaatcttatc ttagaatttt agataaagaa accggctcat cttttgttaa catgattaag tccgttatta aaaatgcaga agagtatttt gacggcaaag caaatgagtc tgagcttgga attaaagctc ttgatgaaaa aactttagaa ataacgctaa aatctcnaaa gccatatttt cttgatatgt tagtacatca aacatttatt cctgtaccaa tgcacgttat tgaaaagtat gggcaaaggt ggacagaccc cgagaatatg gttgttagtg gtcctttcaa attaaaatct agagttttaa atgaaaaggt tgttcttgaa aagaataata aatattataa ttccaaagat gttgttcttg acagtattat attttttgtc acagataata gcattacagc ttataatatg tatttaaatg atgagctgga tgcaattttt aagaatgttc caccagattt gcttaaggat cttaagctta gggacgatta ttattcaatg ggtattaatt caacctcttt ttattctttg aacatgaaag taaaaccgct tgacaatgtt aaagttagaa aggcgctgtc ttttgctatt gatagaaaaa ccttaacaga gagcgttctt aatgatagtt ctattcctac aagaagagca actccagatt atattgatta ctcttataaa agcaatttga gcttatttga tgctgaaatg gcaaagaagc ttttggcaga tgcaggatat cccaatggta ataattttcc tttattaaaa gtaaagtaca atacaagtga tagccagaga aaaattgctg aatttattca aaatcagtgg aagaaaaact taaatattaa tgtacagctt gagaatgaag aatggtcaac atatataaat agtagagtaa atggtaatta tgaaataata agatcaggat ggtcaggaga ttatgctgat cctatgacgt tcttaagcat ctttcaaact gaaaatacat ctttttcatc ttatggatat tcaaattctg aatatgatga acttttaata aaatcagata atgagagaga tatttttaaa agacaggaaa ttctaaaaaa agcagaagca ataataattg aaagagattt tccggctgta tttcttaata taacttcttc tagttatctt tttagaaacg acaagtggaa aggttgggag ccaaatattt cggaaagatt taatttatct gaaataaaac caattaaat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNESNRNKL VFKLNIGSEP ATLDAQLIND TVGSGIVSQM FLGILDGDPR TGGYRPGLAK SWDISDDGVV YTFHLRDNLV WSDGVSITAE GIRKSYLRIL DKETGSSFVN MIKSVIKNAE EYFDGKANES ELGIKALDEK TLEITLKSPK PYFLDMLVHQ TFIPVPMHVI EKYGQRWTDP ENMVVSGPFK LKSRVLNEKV VLEKNNKYYN SKDVVLDSII FFVTDNSITA YNMYLNDELD AIFKNVPPDL LKDLKLRDDY YSMGINSTSF YSLNMKVKPL DNVKVRKALS FAIDRKTLTE SVLNDSSIPT RRATPDYIDY SYKSNLSLFD AEMAKKLLAD AGYPNGNNFP LLKVKYNTSD SQRKIAEFIQ NQWKKNLNIN VQLENEEWST YINSRVNGNY EIIRSGWSGD YADPMTFLSI FQTENTSFSS YGYSNSEYDE LLIKSDNERD IFKRQEILKK AEAIIIERDF PAVFLNITSS SYLFRNDKWK GWEPNISERF NLSEIKPIK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatgaaagt aatagaaaca aattggtttt taagctaaat attggaagtg agcctgctac tttagatgct caattaataa acgatacggt tggatcaggg attgtaagcc aaatgtttct tggcatttta gatggagatc ccaggactgg aggatacaga ccgggacttg ctaaaagttg ggatatttct gatgacggag tagtttatac gtttcattta agagataatc ttgtttggag tgatggagtt tccattactg ccgaaggaat aagaaaatct tatcttagaa ttttagataa agaaaccggc tcatcttttg ttaacatgat taagtccgtt attaaaaatg cagaagagta ttttgacggc aaagcaaatg agtctgagct tggaattaaa gctcttgatg aaaaaacttt agaaataacg ctaaaatctc caaagccata ttttcttgat atgttagtac atcaaacatt tattcctgta ccaatgcacg ttattgaaaa gtatgggcaa aggtggacag accccgagaa tatggttgtt agtggtcctt tcaaattaaa atctagagtt ttaaatgaaa aggttgttct tgaaaagaat aataaatatt ataattccaa agatgttgtt cttgacagta ttatattttt tgtcacagat aatagcatta cagcttataa tatgtattta aatgatgagc tggatgcaat ttttaagaat gttccaccag atttgcttaa ggatcttaag cttagggacg attattattc aatgggtatt aattcaacct ctttttattc tttgaacatg aaagtaaaac cgcttgacaa tgttaaagtt agaaaggcgc tgtcttttgc tattgataga aaaaccttaa cagagagcgt tcttaatgat agttctattc ctacaagaag agcaactcca gattatattg attactctta taaaagcaat ttgagcttat ttgatgctga aatggcaaag aagcttttgg cagatgcagg atatcccaat ggtaataatt ttcctttatt aaaagtaaag tacaatacaa gtgatagcca gagaaaaatt gctgaattta ttcaaaatca gtggaagaaa aacttaaata ttaatgtaca gcttgagaat gaagaatggt caacatatat aaatagtaga gtaaatggta attatgaaat aataagatca ggatggtcag gagattatgc tgatcctatg acgttcttaa gcatctttca aactgaaaat acatcttttt catcttatgg atattcaaat tctgaatatg atgaactttt aataaaatca gataatgaga gagatatttt taaaagacag gaaattctaa aaaaagcaga agcaataata attgaaagag attttccggc tgtatttctt aatataactt cttctagtta tctttttaga aacgacaagt ggaaaggttg ggagccaaat atttcggaaa gatttaattt atctgaaata aaaccaatta aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BobuA.17284.a.A2.PS01128
| PURIFICATION DATe: | 6/24/2011 |
| CONCENTRATION: | 52.64mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNESNRNKL VFKLNIGSEP ATLDAQLIND TVGSGIVSQM FLGILDGDPR TGGYRPGLAK SWDISDDGVV YTFHLRDNLV WSDGVSITAE GIRKSYLRIL DKETGSSFVN MIKSVIKNAE EYFDGKANES ELGIKALDEK TLEITLKSXK PYFLDMLVHQ TFIPVPMHVI EKYGQRWTDP ENMVVSGPFK LKSRVLNEKV VLEKNNKYYN SKDVVLDSII FFVTDNSITA YNMYLNDELD AIFKNVPPDL LKDLKLRDDY YSMGINSTSF YSLNMKVKPL DNVKVRKALS FAIDRKTLTE SVLNDSSIPT RRATPDYIDY SYKSNLSLFD AEMAKKLLAD AGYPNGNNFP LLKVKYNTSD SQRKIAEFIQ NQWKKNLNIN VQLENEEWST YINSRVNGNY EIIRSGWSGD YADPMTFLSI FQTENTSFSS YGYSNSEYDE LLIKSDNERD IFKRQEILKK AEAIIIERDF PAVFLNITSS SYLFRNDKWK GWEPNISERF NLSEIKPIK
Validated NT Sequence
ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgaat gaaagtaata gaaacaaatt ggtttttaag ctaaatattg gaagtgagcc tgctacttta gatgctcaat taataaacga tacggttgga tcagggattg taagccaaat gtttcttggc attttagatg gagatcccag gactggagga tacagaccgg gacttgctaa aagttgggat atttctgatg acggagtagt ttatacgttt catttaagag ataatcttgt ttggagtgat ggagtttcca ttactgccga aggaataaga aaatcttatc ttagaatttt agataaagaa accggctcat cttttgttaa catgattaag tccgttatta aaaatgcaga agagtatttt gacggcaaag caaatgagtc tgagcttgga attaaagctc ttgatgaaaa aactttagaa ataacgctaa aatctcnaaa gccatatttt cttgatatgt tagtacatca aacatttatt cctgtaccaa tgcacgttat tgaaaagtat gggcaaaggt ggacagaccc cgagaatatg gttgttagtg gtcctttcaa attaaaatct agagttttaa atgaaaaggt tgttcttgaa aagaataata aatattataa ttccaaagat gttgttcttg acagtattat attttttgtc acagataata gcattacagc ttataatatg tatttaaatg atgagctgga tgcaattttt aagaatgttc caccagattt gcttaaggat cttaagctta gggacgatta ttattcaatg ggtattaatt caacctcttt ttattctttg aacatgaaag taaaaccgct tgacaatgtt aaagttagaa aggcgctgtc ttttgctatt gatagaaaaa ccttaacaga gagcgttctt aatgatagtt ctattcctac aagaagagca actccagatt atattgatta ctcttataaa agcaatttga gcttatttga tgctgaaatg gcaaagaagc ttttggcaga tgcaggatat cccaatggta ataattttcc tttattaaaa gtaaagtaca atacaagtga tagccagaga aaaattgctg aatttattca aaatcagtgg aagaaaaact taaatattaa tgtacagctt gagaatgaag aatggtcaac atatataaat agtagagtaa atggtaatta tgaaataata agatcaggat ggtcaggaga ttatgctgat cctatgacgt tcttaagcat ctttcaaact gaaaatacat ctttttcatc ttatggatat tcaaattctg aatatgatga acttttaata aaatcagata atgagagaga tatttttaaa agacaggaaa ttctaaaaaa agcagaagca ataataattg aaagagattt tccggctgta tttcttaata taacttcttc tagttatctt tttagaaacg acaagtggaa aggttgggag ccaaatattt cggaaagatt taatttatct gaaataaaac caattaaat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNESNRNKL VFKLNIGSEP ATLDAQLIND TVGSGIVSQM FLGILDGDPR TGGYRPGLAK SWDISDDGVV YTFHLRDNLV WSDGVSITAE GIRKSYLRIL DKETGSSFVN MIKSVIKNAE EYFDGKANES ELGIKALDEK TLEITLKSPK PYFLDMLVHQ TFIPVPMHVI EKYGQRWTDP ENMVVSGPFK LKSRVLNEKV VLEKNNKYYN SKDVVLDSII FFVTDNSITA YNMYLNDELD AIFKNVPPDL LKDLKLRDDY YSMGINSTSF YSLNMKVKPL DNVKVRKALS FAIDRKTLTE SVLNDSSIPT RRATPDYIDY SYKSNLSLFD AEMAKKLLAD AGYPNGNNFP LLKVKYNTSD SQRKIAEFIQ NQWKKNLNIN VQLENEEWST YINSRVNGNY EIIRSGWSGD YADPMTFLSI FQTENTSFSS YGYSNSEYDE LLIKSDNERD IFKRQEILKK AEAIIIERDF PAVFLNITSS SYLFRNDKWK GWEPNISERF NLSEIKPIK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATGAAAGT AATAGAAACA AATTGGTTTT TAAGCTAAAT ATTGGAAGTG AGCCTGCTAC TTTAGATGCT CAATTAATAA ACGATACGGT TGGATCAGGG ATTGTAAGCC AAATGTTTCT TGGCATTTTA GATGGAGATC CCAGGACTGG AGGATACAGA CCGGGACTTG CTAAAAGTTG GGATATTTCT GATGACGGAG TAGTTTATAC GTTTCATTTA AGAGATAATC TTGTTTGGAG TGATGGAGTT TCCATTACTG CCGAAGGAAT AAGAAAATCT TATCTTAGAA TTTTAGATAA AGAAACCGGC TCATCTTTTG TTAACATGAT TAAGTCCGTT ATTAAAAATG CAGAAGAGTA TTTTGACGGC AAAGCAAATG AGTCTGAGCT TGGAATTAAA GCTCTTGATG AAAAAACTTT AGAAATAACG CTAAAATCTC CAAAGCCATA TTTTCTTGAT ATGTTAGTAC ATCAAACATT TATTCCTGTA CCAATGCACG TTATTGAAAA GTATGGGCAA AGGTGGACAG ACCCCGAGAA TATGGTTGTT AGTGGTCCTT TCAAATTAAA ATCTAGAGTT TTAAATGAAA AGGTTGTTCT TGAAAAGAAT AATAAATATT ATAATTCCAA AGATGTTGTT CTTGACAGTA TTATATTTTT TGTCACAGAT AATAGCATTA CAGCTTATAA TATGTATTTA AATGATGAGC TGGATGCAAT TTTTAAGAAT GTTCCACCAG ATTTGCTTAA GGATCTTAAG CTTAGGGACG ATTATTATTC AATGGGTATT AATTCAACCT CTTTTTATTC TTTGAACATG AAAGTAAAAC CGCTTGACAA TGTTAAAGTT AGAAAGGCGC TGTCTTTTGC TATTGATAGA AAAACCTTAA CAGAGAGCGT TCTTAATGAT AGTTCTATTC CTACAAGAAG AGCAACTCCA GATTATATTG ATTACTCTTA TAAAAGCAAT TTGAGCTTAT TTGATGCTGA AATGGCAAAG AAGCTTTTGG CAGATGCAGG ATATCCCAAT GGTAATAATT TTCCTTTATT AAAAGTAAAG TACAATACAA GTGATAGCCA GAGAAAAATT GCTGAATTTA TTCAAAATCA GTGGAAGAAA AACTTAAATA TTAATGTACA GCTTGAGAAT GAAGAATGGT CAACATATAT AAATAGTAGA GTAAATGGTA ATTATGAAAT AATAAGATCA GGATGGTCAG GAGATTATGC TGATCCTATG ACGTTCTTAA GCATCTTTCA AACTGAAAAT ACATCTTTTT CATCTTATGG ATATTCAAAT TCTGAATATG ATGAACTTTT AATAAAATCA GATAATGAGA GAGATATTTT TAAAAGACAG GAAATTCTAA AAAAAGCAGA AGCAATAATA ATTGAAAGAG ATTTTCCGGC TGTATTTCTT AATATAACTT CTTCTAGTTA TCTTTTTAGA AACGACAAGT GGAAAGGTTG GGAGCCAAAT ATTTCGGAAA GATTTAATTT ATCTGAAATA AAACCAATTA AATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA