BobuA.17194.p

LysM domain protein (BB_0323)

CENTER ID: BobuA.17194.p
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17194.p.B2.GE39127 mature protein 21 377
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.719
RefSeq: NP_212457.1
UniProt: O51302

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIKNKLISL LIVVAISFIA CKTPPESRES KNAKIAQPDN KNFQLRDIKD IKNELIRERG HLFYSKEFNE AERLEEAMKQ SFSKKKAIEG NEIALKVLER YKTIIRETRE KKEKTNYLKE NIEKYLNDAE ANEAYIWIPL EIDEVNNLYF EATRKYKNYD LDNALDMYSK AFNRAQQAAK NAKEAKALKE TDERMYKQLK ALEAASNLPI YSNNKLIKPS PWNGRAFIKE RNSHLNLLNT NKDTYLLGEA EISIPIVLAY EEKVEIAKNS KPQEQFKTLE LIERSRTLWE KGVEAKNVKN FRLANELFLE SARYLEAYQS NASSELYVIK IGNTLWGISK KLYNDPYLWP KIWFANRQKI QNPDLIHSNW KIIIPAK
NT Sequence
atgaatataa agaataaatt aatatcgctg ctaattgtag tagcaatctc ttttatcgca tgcaaaacgc ctccagaatc aagagagagt aaaaatgcta aaattgcaca accagataat aaaaattttc aattaagaga tataaaagac attaaaaatg aactaataag agaacgggga catctttttt attccaaaga gtttaatgaa gctgaaagat tagaagaagc aatgaaacaa agtttttcca aaaaaaaagc aatagaagga aatgaaattg ctctaaaagt acttgaaaga tacaaaacaa taataagaga aacgagggaa aaaaaagaaa aaacaaatta ccttaaagaa aatattgaaa agtatctaaa cgatgcagaa gcaaatgaag catacatatg gatcccgctg gaaatcgacg aagtaaataa tttatatttt gaagcaacaa gaaaatataa aaattacgat cttgataatg cccttgacat gtatagcaag gcctttaaca gagcacaaca agcagcaaaa aatgccaaag aagctaaagc tttaaaagaa actgatgaga gaatgtacaa acaattaaaa gcacttgaag cggcttccaa cttaccaatt tatagcaata ataagcttat aaagccatct ccatggaatg gtagagcatt cattaaagaa agaaacagtc acttaaacct tttaaatact aataaagaca cttatcttct tggagaagca gaaatttcaa ttccaatagt actcgcatac gaagaaaaag tggaaatagc aaaaaactcc aaacctcaag aacaattcaa aacgctagaa cttattgaaa gatctagaac attgtgggaa aaaggggttg aggctaaaaa tgtaaaaaac tttagacttg ccaatgaatt atttttagaa tctgcaagat accttgaagc ttatcaaagc aatgcaagca gcgagcttta cgtaataaaa ataggcaata ccctatgggg catttccaaa aaattataca acgatcctta cttatggcca aaaatttggt ttgccaacag acaaaaaata caaaatccgg atctaattca ttctaactgg aagataataa ttcctgccaa ataa
Details for BobuA.17194.p.B2.GE39127
HARVESTED ON: 5/6/2015
SEQUENCED ON: 5/6/2015
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMC KTPPESRESK NAKIAQPDNK NFQLRDIKDI KNELIRERGH LFYSKEFNEA ERLEEAMKQS FSKKKAIEGN EIALKVLERY KTIIRETREK KEKTNYLKEN IEKYLNDAEA NEAYIWIPLE IDEVNNLYFE ATRKYKNYDL DNALDMYSKA FNRAQQAAKN AKEAKALKET DERMYKQLKA LEAASNLPIY SNNKLIKPSP WNGRAFIKER NSHLNLLNTN KDTYLLGEAE ISIPIVLAYE EKVEIAKNSK PQEQFKTLEL IERSRTLWEK GVEAKNVKNF RLANELFLES ARYLEAYQSN ASSELYVIKI GNTLWGISKK LYNDPYLWPK IWFANRQKIQ NPDLIHS
Validated NT Sequence
tagaatgaat tagatccgga ttttgtattt tttgtctgtt ggcaaaccaa atttttggcc ataagtaagg atcgttgtat aattttttgg aaatgcccca tagggtattg cctattttta ttacgtaaag ctcgctgctt gcattgcttt gataagcttc aaggtatctt gcagattcta aaaataattc attggcaagt ctaaagtttt ttacattttt agcctcaacc cctttttccc acaatgttct agatctttca ataagttcta gcgttttgaa ttgttcttga ggtttggagt tttttgctat ttccactttt tcttcgtatg cgagtactat tggaattgaa atttctgctt ctccaagaag ataagtgtct ttattagtat ttaaaaggtt taagtgactg tttctttctt taatgaatgc tctaccattc catggagatg gctttataag cttattattg ctataaattg gtaagttgga agccgcttca agtgctttta attgtttgta cattctctca tcagtttctt ttaaagcttt agcttctttg gcattttttg ctgcttgttg tgctctgtta aaggccttgc tatacatgtc aagggcatta tcaagatcgt aatttttata ttttcttgtt gcttcaaaat ataaattatt tacttcgtcg atttccagcg ggatccatat gtatgcttca tttgcttctg catcgtttag atacttttca atattttctt taaggtaatt tgttttttct tttttttccc tcgtttctct tattattgtt ttgtatcttt caagtacttt tagagcaatt tcatttcctt ctattgcttt ttttttggaa aaactttgtt tcattgcttc ttctaatctt tcagcttcat taaactcttt ggaataaaaa agatgtcccc gttctcttat tagttcattt ttaatgtctt ttatatctct taattgaaaa tttttattat ctggttgtgc aattttagca tttttactct ctcttgattc tggaggcgtt ttgcacatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHCK TPPESRESKN AKIAQPDNKN FQLRDIKDIK NELIRERGHL FYSKEFNEAE RLEEAMKQSF SKKKAIEGNE IALKVLERYK TIIRETREKK EKTNYLKENI EKYLNDAEAN EAYIWIPLEI DEVNNLYFEA TRKYKNYDLD NALDMYSKAF NRAQQAAKNA KEAKALKETD ERMYKQLKAL EAASNLPIYS NNKLIKPSPW NGRAFIKERN SHLNLLNTNK DTYLLGEAEI SIPIVLAYEE KVEIAKNSKP QEQFKTLELI ERSRTLWEKG VEAKNVKNFR LANELFLESA RYLEAYQSNA SSELYVIKIG NTLWGISKKL YNDPYLWPKI WFANRQKIQN PDLIHSNWKI IIPAK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattgcaaaa cgcctccaga atcaagagag agtaaaaatg ctaaaattgc acaaccagat aataaaaatt ttcaattaag agatataaaa gacattaaaa atgaactaat aagagaacgg ggacatcttt tttattccaa agagtttaat gaagctgaaa gattagaaga agcaatgaaa caaagttttt ccaaaaaaaa agcaatagaa ggaaatgaaa ttgctctaaa agtacttgaa agatacaaaa caataataag agaaacgagg gaaaaaaaag aaaaaacaaa ttaccttaaa gaaaatattg aaaagtatct aaacgatgca gaagcaaatg aagcatacat atggatcccg ctggaaatcg acgaagtaaa taatttatat tttgaagcaa caagaaaata taaaaattac gatcttgata atgcccttga catgtatagc aaggccttta acagagcaca acaagcagca aaaaatgcca aagaagctaa agctttaaaa gaaactgatg agagaatgta caaacaatta aaagcacttg aagcggcttc caacttacca atttatagca ataataagct tataaagcca tctccatgga atggtagagc attcattaaa gaaagaaaca gtcacttaaa ccttttaaat actaataaag acacttatct tcttggagaa gcagaaattt caattccaat agtactcgca tacgaagaaa aagtggaaat agcaaaaaac tccaaacctc aagaacaatt caaaacgcta gaacttattg aaagatctag aacattgtgg gaaaaagggg ttgaggctaa aaatgtaaaa aactttagac ttgccaatga attattttta gaatctgcaa gataccttga agcttatcaa agcaatgcaa gcagcgagct ttacgtaata aaaataggca ataccctatg gggcatttcc aaaaaattat acaacgatcc ttacttatgg ccaaaaattt ggtttgccaa cagacaaaaa atacaaaatc cggatctaat tcattctaac tggaagataa taattcctgc caaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat