BobuA.17194.j

BB_C01

CENTER ID: BobuA.17194.j
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17194.j.B1.GE38624 full length 1 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.242
RefSeq: NP_051407.1
UniProt: Q9S067

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKGFLNNAKS PTCYNKHQHK LISLTSTLDY LNKKDKKYTQ QNILYYFNEN LKRNGLAPTT LRTMQNYLYK LEKVLKVTTN YYQHLGVNCG TEIYYKLKYP KKECYQKINK YFKERKNSRF KSRVNKHLKD NVSKNGSVNS VECLSNKNNI KEERKIKEIE KYQLRNYFNN CNFKTEEALS ILYLNIDKDT KIEAINILKQ NEIALIKKFN IKKSCMKEKQ KKLKEILYNT RKKLEENGYN PKQLEINLQK VYENYKYKPH FIIENHKYSD LNNIKRKLEK SIKRKKENSQ QNYQNLKENI FNILIEQLKK ETNIEILKPI VKEYLNNQKK IEYNKVFGTY HLELSEIIKN EKNSLTTEEF SIKAV
NT Sequence
atgaaaggtt ttctaaataa tgcgaaaagt ccaacttgct acaacaaaca ccaacacaaa ttaatatctc ttacttcaac actagattat ctaaacaaaa aagacaagaa atacacgcaa caaaacatac tctattactt taatgaaaat ttaaaaagaa atgggctagc tcccactaca ctaagaacaa tgcaaaatta tctttacaaa ttagaaaaag tattaaaagt cacaactaat tactaccaac acttaggtgt aaattgtgga actgaaattt actataagct aaagtatcct aaaaaagaat gttaccagaa aatcaacaag tactttaaag aacgaaaaaa ctctagattt aaatctagag ttaataaaca tttaaaagac aatgtttcta aaaatggtag tgtaaattca gtggagtgtt taagtaataa aaataatata aaagaagaaa gaaagattaa agaaatagaa aaatatcaac taagaaatta tttcaataat tgtaacttta aaacggaaga agctctttct attttgtatt taaatattga taaagatact aagattgagg caataaatat cttaaaacaa aatgaaattg ccctaataaa aaaattcaat ataaaaaaat cttgcatgaa agaaaaacaa aaaaaattaa aagaaattct atacaacact aggaaaaaat tagaagaaaa cgggtacaat cctaaacaat tagaaataaa tttacaaaaa gtatacgaaa attacaaata taagccccat tttattattg aaaatcataa atatagcgat ttaaacaata taaagcgtaa attagaaaag tcgattaaaa gaaaaaaaga aaattctcaa caaaattatc aaaatttaaa ggaaaatatt ttcaatatcc ttattgaaca actaaaaaaa gaaacaaata ttgaaattct aaagcctatt gtaaaagaat atttgaataa ccaaaagaaa atagaataca ataaagtatt tggtacatat catcttgaat tatcagaaat aataaaaaat gaaaaaaatt ctttaaccac agaagaattt agcataaagg ccgtataa
Details for BobuA.17194.j.B1.GE38624
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 45kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 95
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMK GFLNITKIPT CHNKHQHKLI SLTSTLDYLN KKDKKYTQQN ILYCFNENLK RNGLAPTTLR TMQNYLYKLE KVLKVTTNYY QHMGVNCGTE IYYKLKYPKK ECYQKINKYF KEQKNSRFKS RVNKHFKDNV SKNSSVNSVG CLSNKNNIKE ERKIKEIEKY QLRNYFNNCN FKTEEALSIL YLNIDKDTKI EAINILKQNE IALIKKFNIK KSCMKEKQKK LKEILYNTRK KLEENGYNPK QLEINLQKVY ENYKYKPHFI IENHKYSDLN NIKRKLEKSI ERKKENSQQN YQNLKANIFN ILIEQLKKET NIEILKTIVK EYLNNQKKIE YNKVFGIYYL ELLEIIKNEK NSLTT
Validated NT Sequence
tctgtggtta aagaattttt ttcatttttt attatttcta ataattcaag ataatatata ccaaatactt tattgtattc tattttcttt tggttattca aatattcttt tacaatagtc tttagaattt caatatttgt ttcttttttt agttgttcaa taaggatatt gaaaatgttt gcctttaaat tttgataatt ttgttgagaa ttttcttttt ttctttcaat cgacttttct aatttacgct ttatattgtt taaatcgcta tatttatgat tttcaataat aaaatggggc ttatatttgt aattttcgta tactttttgt aaatttattt ctaattgttt gggattgtac ccgttttctt ctaatttttt cctagtgttg tatagaattt cttttaattt tttttgtttt tctttcatgc aagatttttt tatattgaat ttttttatta gggcaatttc attttgtttt aagatattta ttgcctcaat cttagtatct ttatcaatat ttaaatacaa aatagaaaga gcttcttccg ttttaaagtt acaattattg aaataatttc ttagttgata tttttctatt tctttaatct ttctttcttc ttttatatta tttttattac ttaaacaccc cactgaattt acactactat ttttggaaac attgtcttta aaatgtttat taactctaga tttaaatcta gagttttttt gctctttaaa gtacttgttg attttctggt aacactcttt tttaggatac tttagcttat agtaaatttc agttccacaa tttacaccca tgtgttggta gtaattagtt gtaactttta atactttttc taatttgtaa agataatttt gcattgttct tagtgtagtg ggagctagac cattcctttt tagattttcg ttaaagcagt agagtatgtt ttgttgcgtg tatttcttat cttttttgtt taggtaatct agcgttgaag taagagatat taatttgtgt tggtgtttgt tgtgacaagt tggaattttt gtgatattta gaaaaccttt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMK GFLNNAKSPT CYNKHQHKLI SLTSTLDYLN KKDKKYTQQN ILYYFNENLK RNGLAPTTLR TMQNYLYKLE KVLKVTTNYY QHLGVNCGTE IYYKLKYPKK ECYQKINKYF KERKNSRFKS RVNKHLKDNV SKNGSVNSVE CLSNKNNIKE ERKIKEIEKY QLRNYFNNCN FKTEEALSIL YLNIDKDTKI EAINILKQNE IALIKKFNIK KSCMKEKQKK LKEILYNTRK KLEENGYNPK QLEINLQKVY ENYKYKPHFI IENHKYSDLN NIKRKLEKSI KRKKENSQQN YQNLKENIFN ILIEQLKKET NIEILKPIVK EYLNNQKKIE YNKVFGTYHL ELSEIIKNEK NSLTTEEFSI KAV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag gttttctaaa taatgcgaaa agtccaactt gctacaacaa acaccaacac aaattaatat ctcttacttc aacactagat tatctaaaca aaaaagacaa gaaatacacg caacaaaaca tactctatta ctttaatgaa aatttaaaaa gaaatgggct agctcccact acactaagaa caatgcaaaa ttatctttac aaattagaaa aagtattaaa agtcacaact aattactacc aacacttagg tgtaaattgt ggaactgaaa tttactataa gctaaagtat cctaaaaaag aatgttacca gaaaatcaac aagtacttta aagaacgaaa aaactctaga tttaaatcta gagttaataa acatttaaaa gacaatgttt ctaaaaatgg tagtgtaaat tcagtggagt gtttaagtaa taaaaataat ataaaagaag aaagaaagat taaagaaata gaaaaatatc aactaagaaa ttatttcaat aattgtaact ttaaaacgga agaagctctt tctattttgt atttaaatat tgataaagat actaagattg aggcaataaa tatcttaaaa caaaatgaaa ttgccctaat aaaaaaattc aatataaaaa aatcttgcat gaaagaaaaa caaaaaaaat taaaagaaat tctatacaac actaggaaaa aattagaaga aaacgggtac aatcctaaac aattagaaat aaatttacaa aaagtatacg aaaattacaa atataagccc cattttatta ttgaaaatca taaatatagc gatttaaaca atataaagcg taaattagaa aagtcgatta aaagaaaaaa agaaaattct caacaaaatt atcaaaattt aaaggaaaat attttcaata tccttattga acaactaaaa aaagaaacaa atattgaaat tctaaagcct attgtaaaag aatatttgaa taaccaaaag aaaatagaat acaataaagt atttggtaca tatcatcttg aattatcaga aataataaaa aatgaaaaaa attctttaac cacagaagaa tttagcataa aggccgtatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat