BobuA.17194.h

BB_C01

CENTER ID: BobuA.17194.h
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17194.h.B1.GE38623 full length 1 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.200
RefSeq: NP_051363.1
UniProt: Q9S081

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKGFPKNTKS PTCHNKHQHK LISLTSTLDY LNKKDKKYNQ KNILYYYNEN LKRNGLAPTT LRTMQNYLYK LEKVLKVTTN YYQHMGVNCG TEIYYKLKYP KKECYQKINK YFKERKNSRF KSRVNTHFKD NVSKNSSVNS VECLSNKNNI KEERKIKEIE KYQLRNYFNN CNFKTEEALS ILYLNIDKDT KIEAINILKQ NEIALIKKFN IKKSCMKEKQ KKLKEILYNT RKKLEENGYN PKQLEINLQK VYENYKYKPH FIIENHKYSD LNNIKRKLEK SIERKKENSQ QNYQNLKENI FNILIEQLKK ETNIEILKPI VKEYLNNQKK IEYNKVFGTY HLELSEIIKN EKNSLTTEEF SIKAV
NT Sequence
ttgaaaggtt ttcctaaaaa tacaaaaagt ccaacttgcc acaacaaaca ccaacataaa ttaatatctc ttacttcaac actagattat ctaaacaaaa aagataaaaa atataaccaa aaaaacatac tctattacta taatgaaaat ttaaaaagaa atggtctagc tccaactaca ctaagaacaa tgcaaaatta tctttacaaa ttagaaaaag tattaaaagt tacaactaat tactaccaac acatgggtgt aaattgtgga actgaaattt actataagct aaagtatcct aaaaaagaat gttaccagaa aatcaacaag tactttaaag aacgaaaaaa ctctagattt aaatctagag ttaataccca ttttaaagac aatgtttcca aaaatagtag tgtaaattca gtggagtgtt taagtaataa aaataatata aaagaagaaa gaaagattaa agaaatagaa aaatatcaac taagaaatta tttcaataat tgtaacttta aaacggaaga agctctttct attttgtatt taaatattga taaagatact aagattgagg caataaatat cttaaaacaa aatgaaattg ccctaataaa aaaattcaat ataaaaaaat cttgcatgaa agaaaaacaa aaaaaattaa aagaaattct atacaacact aggaaaaaat tagaagaaaa cgggtacaat cccaaacaat tagaaataaa tttacaaaaa gtatacgaaa attacaaata taagccccac tttattattg aaaatcataa atatagcgat ttaaacaata taaagcgtaa attagaaaag tcgattgaaa gaaaaaaaga aaattctcaa caaaattatc aaaatttaaa ggaaaatatt ttcaatatcc ttattgaaca actaaaaaaa gaaacaaata ttgaaattct aaagcctatt gtaaaagaat atttgaataa ccaaaagaaa atagaataca ataaagtatt tggtacatat catcttgaat tatcagaaat aataaaaaat gaaaaaaatt ctttaaccac agaagaattt agcataaagg ccgtataa
Details for BobuA.17194.h.B1.GE38623
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 45kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK GFPKNTKSPT CHNKHQHKLI SLTSTLDYLN KKDKKYNQKN ILYYYNENLK RNGLAPTTLR TMQNYLYKLE KVLKVTTNYY QHMGVNCGTE IYYKLKYPKK ECYQKINKYF KERKNSRFKS RVNTHFKDNV SKNSSVNSVE CLSNKNNIKE ERKIKEIEKY QLRNYFNNCN FKTEEALSIL YLNIDKDTKI EAINILKQNE IALIKKFNIK KSCMKEKQKK LKEILYNTRK KLEENGYNPK QLEINLQKVY ENYKYKPHFI IENHKYSDLN NIKRKLEKSI ERKKENSQQN YQNLKENIFN ILIEQLKKET NIEILKPIVK EYLNNQKKIE YNKVFGTYHL ELSEIIKNEK NSLTTEEFSI KAV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaaag gttttcctaa aaatacaaaa agtccaactt gccacaacaa acaccaacat aaattaatat ctcttacttc aacactagat tatctaaaca aaaaagataa aaaatataac caaaaaaaca tactctatta ctataatgaa aatttaaaaa gaaatggtct agctccaact acactaagaa caatgcaaaa ttatctttac aaattagaaa aagtattaaa agttacaact aattactacc aacacatggg tgtaaattgt ggaactgaaa tttactataa gctaaagtat cctaaaaaag aatgttacca gaaaatcaac aagtacttta aagaacgaaa aaactctaga tttaaatcta gagttaatac ccattttaaa gacaatgttt ccaaaaatag tagtgtaaat tcagtggagt gtttaagtaa taaaaataat ataaaagaag aaagaaagat taaagaaata gaaaaatatc aactaagaaa ttatttcaat aattgtaact ttaaaacgga agaagctctt tctattttgt atttaaatat tgataaagat actaagattg aggcaataaa tatcttaaaa caaaatgaaa ttgccctaat aaaaaaattc aatataaaaa aatcttgcat gaaagaaaaa caaaaaaaat taaaagaaat tctatacaac actaggaaaa aattagaaga aaacgggtac aatcccaaac aattagaaat aaatttacaa aaagtatacg aaaattacaa atataagccc cactttatta ttgaaaatca taaatatagc gatttaaaca atataaagcg taaattagaa aagtcgattg aaagaaaaaa agaaaattct caacaaaatt atcaaaattt aaaggaaaat attttcaata tccttattga acaactaaaa aaagaaacaa atattgaaat tctaaagcct attgtaaaag aatatttgaa taaccaaaag aaaatagaat acaataaagt atttggtaca tatcatcttg aattatcaga aataataaaa aatgaaaaaa attctttaac cacagaagaa tttagcataa aggccgtatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat