BobuA.12797.a

xylose operon regulatory protein (xylR-1) (BB_0693)

CENTER ID: BobuA.12797.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.12797.a.B1.GE39126 full length 1 402
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1084
RefSeq: NP_212827.1
UniProt: O51636

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQGENMVSIR GGNRRKILLS LKNMQYSRTD LARKLSLTNA AVTILTNQMI KENLLIEVGS RVSDVKKHGR KEILLDINKD YAYSMGVIIS SNYFQIGIAN LKCEVLISET HSFEPPVSAY DILEKIKDHM IEIIWKHNFS RDKFIGLGFS ITGLIKDKEL GIVNDSYGSW IEKDVPVKRI LEEYFSLTVY LESYVKNLSL AEFMGKNIDN IMFFDYTDTA ELSIWSGGNV YPGFNNKSGM VSHMIIDYEG EKNCPTCGNK GCVNMLISNF ALQRLISKEF MNGEIPELYE KYEGRLKKVT IYDIFSLYEK YDFINKIMQD TVKYLAIIII NIQRMLDFNY LVLYGQSFKL KAFFDLLKEE IKKLNKENIV LKLSSLDTEV SVVGPASSVI FNKFYLTGGD ID
NT Sequence
atgcagggtg aaaatatggt ttcaattaga ggcggaaata gaagaaaaat tcttcttagt ttgaaaaata tgcaatattc aagaacagac ctggctcgta agttaagctt gacaaatgct gcagttacta ttttgactaa tcaaatgata aaagaaaatc ttttgattga agttggctct agggtgtctg atgttaaaaa acatggacga aaagaaatac ttctcgatat taataaagat tatgcgtatt caatgggagt tattatttct agcaattatt ttcaaatagg tattgccaat cttaaatgcg aggttttaat aagcgagact cattcttttg agcccccagt tagcgcttat gatattttag aaaaaataaa agatcatatg atagaaatta tttggaaaca taatttctca agagataagt ttattggctt aggctttagt attacagggt taataaagga taaagaattg ggcattgtta atgatagcta tggatcgtgg attgaaaaag atgttcctgt taagagaata cttgaggaat acttttcact aacagtttat cttgaaagtt atgttaaaaa tttatctctt gctgaattta tgggtaaaaa tatagataat attatgtttt ttgactacac agatactgct gaactttcga tttggtcagg cggcaatgtt taccctggtt ttaataataa atcaggtatg gttagtcaca tgataattga ttatgaggga gaaaaaaatt gtccaacttg tggtaataag ggttgtgtca atatgctaat atctaatttt gctttgcaga gattgatttc caaagaattt atgaatggtg agattcctga gctttatgaa aagtatgagg gcagattgaa aaaggttaca atatatgaca ttttttctct ttatgaaaaa tatgatttta taaataaaat tatgcaagat actgttaaat atttggcaat aattattatt aatattcaaa gaatgcttga ttttaattat ttagtactct atggtcaaag ttttaaatta aaagcttttt ttgatttgtt aaaagaagag attaaaaagc tcaataaaga gaatatagta ttaaagctta gttcattaga tactgaagtt tctgttgttg ggcccgcttc cagtgttatt tttaataaat tttatttgac tggaggagat attgattaa
Details for BobuA.12797.a.B1.GE39126
HARVESTED ON: 5/6/2015
SEQUENCED ON: 5/6/2015
EXPECTED MW: 47kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMQ GENMVSIRGG NRRKILLXLK NMQYSRTDLA RKLSLTNAAV TILTNQMIKE NLLIEVGSRV SDVKKHGRKE ILLDINKDYA YSMGVIISSN YFQIGIANLK CEVLISETHS FEPPVSAYDI LEKIKDHMIE IIWKHNFSRD KFIGLGFSIT GLIKDKELGI VNDSYGSWIE KDVPVKRILE EYFSLTVYLE SYVKNLSLAE FMGKNIDNIM FFDYTDTAEL SIWSGGNVYP GFNNKSGMVS HMIIDYEGEK NCPTCGNKGC VNMLISNFAL QRLISKEFMN GEIPELYEKY EGRLKKVTIY DIFSLYEKYD FINKIMQDTV KYLAIIIINI QRMLDFNYLV LYGQSFKLKA FFDLLKEEIK KLNKENIVLK LSSLDTEVSV VGPASSVIFN K
Validated NT Sequence
aatttattaa aaataacact ggaagcgggc ccaacaacag aaacttcagt atctaatgaa ctaagcttta atactatatt ctctttattg agctttttaa tctcttcttt taacaaatca aaaaaagctt ttaatttaaa actttgacca tagagtacta aataattaaa atcaagcatt ctttgaatat taataataat tattgccaaa tatttaacag tatcttgcat aattttattt ataaaatcat atttttcata aagagaaaaa atgtcatata ttgtaacctt tttcaatctg ccctcatact tttcataaag ctcaggaatc tcaccattca taaattcttt ggaaatcaat ctctgcaaag caaaattaga tattagcata ttgacacaac ccttattacc acaagttgga caattttttt ctccctcata atcaattatc atgtgactaa ccatacctga tttattatta aaaccagggt aaacattgcc gcctgaccaa atcgaaagtt cagcagtatc tgtgtagtca aaaaacataa tattatctat atttttaccc ataaattcag caagagataa atttttaaca taactttcaa gataaactgt tagtgaaaag tattcctcaa gtattctctt aacaggaaca tctttttcaa tccacgatcc atagctatca ttaacaatgc ccaattcttt atcctttatt aaccctgtaa tactaaagcc taagccaata aacttatctc ttgagaaatt atgtttccaa ataatttcta tcatatgatc ttttattttt tctaaaatat cataagcgct aactgggggc tcaaaagaat gagtctcgct tattaaaacc tcgcatttaa gattggcaat acctatttga aaataattgc tagaaataat aactcccatt gaatacgcat aatctttatt aatatcgaga agtatttctt ttcgtccatg ttttttaaca tcagacaccc tagagccaac ttcaatcaaa agattttctt ttatcatttg attagtcaaa atagtaactg cagcatttgt caagcttaac ttacgagcca ggtctgttct tgaatattgc atatttttca annnaagaag aatttttctt ctatttccgc ctctaattga aaccatattt tcaccctgca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMQ GENMVSIRGG NRRKILLSLK NMQYSRTDLA RKLSLTNAAV TILTNQMIKE NLLIEVGSRV SDVKKHGRKE ILLDINKDYA YSMGVIISSN YFQIGIANLK CEVLISETHS FEPPVSAYDI LEKIKDHMIE IIWKHNFSRD KFIGLGFSIT GLIKDKELGI VNDSYGSWIE KDVPVKRILE EYFSLTVYLE SYVKNLSLAE FMGKNIDNIM FFDYTDTAEL SIWSGGNVYP GFNNKSGMVS HMIIDYEGEK NCPTCGNKGC VNMLISNFAL QRLISKEFMN GEIPELYEKY EGRLKKVTIY DIFSLYEKYD FINKIMQDTV KYLAIIIINI QRMLDFNYLV LYGQSFKLKA FFDLLKEEIK KLNKENIVLK LSSLDTEVSV VGPASSVIFN KFYLTGGDID
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagg gtgaaaatat ggtttcaatt agaggcggaa atagaagaaa aattcttctt agtttgaaaa atatgcaata ttcaagaaca gacctggctc gtaagttaag cttgacaaat gctgcagtta ctattttgac taatcaaatg ataaaagaaa atcttttgat tgaagttggc tctagggtgt ctgatgttaa aaaacatgga cgaaaagaaa tacttctcga tattaataaa gattatgcgt attcaatggg agttattatt tctagcaatt attttcaaat aggtattgcc aatcttaaat gcgaggtttt aataagcgag actcattctt ttgagccccc agttagcgct tatgatattt tagaaaaaat aaaagatcat atgatagaaa ttatttggaa acataatttc tcaagagata agtttattgg cttaggcttt agtattacag ggttaataaa ggataaagaa ttgggcattg ttaatgatag ctatggatcg tggattgaaa aagatgttcc tgttaagaga atacttgagg aatacttttc actaacagtt tatcttgaaa gttatgttaa aaatttatct cttgctgaat ttatgggtaa aaatatagat aatattatgt tttttgacta cacagatact gctgaacttt cgatttggtc aggcggcaat gtttaccctg gttttaataa taaatcaggt atggttagtc acatgataat tgattatgag ggagaaaaaa attgtccaac ttgtggtaat aagggttgtg tcaatatgct aatatctaat tttgctttgc agagattgat ttccaaagaa tttatgaatg gtgagattcc tgagctttat gaaaagtatg agggcagatt gaaaaaggtt acaatatatg acattttttc tctttatgaa aaatatgatt ttataaataa aattatgcaa gatactgtta aatatttggc aataattatt attaatattc aaagaatgct tgattttaat tatttagtac tctatggtca aagttttaaa ttaaaagctt tttttgattt gttaaaagaa gagattaaaa agctcaataa agagaatata gtattaaagc ttagttcatt agatactgaa gtttctgttg ttgggcccgc ttccagtgtt atttttaata aattttattt gactggagga gatattgatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat