SSGCID: Target Page

BobuA.11726.a

hypothetical protein

CENTER ID:	BobuA.11726.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.11726.a.A2.GE27720	Mature protein( BobuA.11726.a )		24	342

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.1057
RefSeq:	NP_212800.1
UniProt:	O51610

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLSFGKMIL LIDLILFSYL SLYSKTPDYL NVLDFFDTNV FRFDFNIEND VFTIENDKGY LKFRVGFEYA LTSSGYYMFV DPIIDIRGEI LISQKVLKQI ENYFSSLKDY NKPRITSIII DPGHGGHDTG AVVTLKINGY DVVLQEKDFA LTYSIYLSKI LSNYFVNKNI LLTRINDVYL TLKERSEFAN AIKPNFPNNV IFLSIHANDA PNGEARGVEF WYLPKDSKRE VIKDFKGYDI KGNRYLSELN DILDIKYKYE SKRLAEILYK VFKNELSETN IRPIRKEQWF VIKNSSMPAV LIEMGFLSNI LDAKLILDYN YMSKFNILIL KALMEFINFY EK

NT Sequence

GTGCCATTGA GCTTTGGGAA AATGATATTA TTGATTGATT TGATTTTGTT TTCATATTTA AGCTTGTATT CTAAGACCCC CGATTATTTA AATGTTCTTG ATTTTTTTGA TACTAATGTT TTTAGGTTTG ATTTTAACAT TGAAAATGAT GTTTTTACAA TTGAAAATGA TAAGGGATAT TTGAAGTTTA GGGTAGGCTT TGAATATGCG CTTACATCTT CTGGTTATTA TATGTTTGTA GACCCAATTA TTGACATTCG TGGAGAAATT TTAATAAGTC AAAAGGTATT AAAACAAATT GAAAATTATT TTAGTTCTCT TAAAGACTAT AATAAACCCA GAATTACTTC AATAATCATT GATCCTGGAC ATGGCGGGCA TGATACTGGT GCTGTTGTGA CTTTAAAGAT AAATGGTTAT GACGTTGTGC TTCAGGAAAA AGATTTTGCA TTAACCTATT CTATATATTT GTCTAAAATT TTAAGTAATT ATTTTGTAAA TAAAAATATT TTGTTAACTC GTATAAATGA TGTTTATTTA ACTTTAAAAG AACGGTCGGA ATTTGCAAAT GCAATAAAGC CAAATTTCCC CAATAATGTT ATATTTTTAT CTATACATGC TAATGATGCT CCAAACGGTG AAGCTAGAGG AGTTGAGTTT TGGTATCTTC CTAAGGATTC AAAAAGAGAA GTTATTAAAG ATTTTAAGGG ATATGATATT AAAGGTAATA GATACTTAAG CGAGCTTAAT GATATACTAG ATATTAAATA TAAATATGAA TCAAAAAGAT TGGCTGAAAT TTTGTATAAA GTGTTTAAAA ATGAGTTAAG CGAAACTAAT ATTAGGCCAA TCAGAAAAGA GCAATGGTTT GTAATAAAAA ACAGCAGTAT GCCTGCTGTG TTGATTGAAA TGGGGTTTTT ATCCAATATT TTAGATGCTA AATTAATTTT GGATTATAAT TACATGAGCA AGTTTAATAT TCTAATACTT AAAGCTTTAA TGGAATTTAT AAATTTTTAT GAAAAA

Details for BobuA.11726.a.A2.GE27720

HARVESTED ON:	11/16/2009
SEQUENCED ON:	11/23/2009
EXPECTED MW:	39kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSKTPDYLN VLDFFDTNVF RFDFNIENDV FTIENDKGYL KFRVGFEYAL TSSGYYMFVD PIIDIRGEIL ISQKVLKQIE NYFSSLKDYN KPRITSIIID PGHGGHDTGA VVTLKINGYD VVLQEKDFAL TYSIYLSKIL SNYFVNKNIL LTRINDVYLT LKERSEFANA IKPNFPNNVI FLSIHANDAP NGEARGVEFW YLPKDSKREV IKDFKGYDIK GNRYLSELND ILDIKYKYES KRLAEILYKV FKNELSETNI RPIRKEQWFV IKNSSMPAVL IEMGFLSNIL DAKLILDYNY MSKFNILILK ALMEFINFY

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcta agacccccga ttatttaaat gttcttgatt tttttgatac taatgttttt aggtttgatt ttaacattga aaatgatgtt tttacaattg aaaatgataa gggatatttg aagtttaggg taggctttga atatgcgctt acatcttctg gttattatat gtttgtagac ccaattattg acattcgtgg agaaatttta ataagtcaaa aggtattaaa acaaattgaa aattatttta gttctcttaa agactataat aaacccagaa ttacttcaat aatcattgat cctggacatg gcgggcatga tactggtgct gttgtgactt taaagataaa tggttatgac gttgtgcttc aggaaaaaga ttttgcatta acctattcta tatatttgtc taaaatttta agtaattatt ttgtaaataa aaatattttg ttaactcgta taaatgatgt ttatttaact ttaaaagaac ggtcggaatt tgcaaatgca ataaagccaa atttccccaa taatgttata tttttatcta tacatgctaa tgatgctcca aacggtgaag ctagaggagt tgagttttgg tatcttccta aggattcaaa aagagaagtt attaaagatt ttaagggata tgatattaaa ggtaatagat acttaagcga gcttaatgat atactagata ttaaatataa atatgaatca aaaagattgg ctgaaatttt gtataaagtg tttaaaaatg agttaagcga aactaatatt aggccaatca gaaaagagca atggtttgta ataaaaaaca gcagtatgcc tgctgtgttg attgaaatgg ggtttttatc caatatttta gatgctaaat taattttgga ttataattac atgagcaagt ttaatattct aatacttaaa gctttaatgg aatttataaa tttttatg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctaagacc cccgattatt taaatgttct tgattttttt gatactaatg tttttaggtt tgattttaac attgaaaatg atgtttttac aattgaaaat gataagggat atttgaagtt tagggtaggc tttgaatatg cgcttacatc ttctggttat tatatgtttg tagacccaat tattgacatt cgtggagaaa ttttaataag tcaaaaggta ttaaaacaaa ttgaaaatta ttttagttct cttaaagact ataataaacc cagaattact tcaataatca ttgatcctgg acatggcggg catgatactg gtgctgttgt gactttaaag ataaatggtt atgacgttgt gcttcaggaa aaagattttg cattaaccta ttctatatat ttgtctaaaa ttttaagtaa ttattttgta aataaaaata ttttgttaac tcgtataaat gatgtttatt taactttaaa agaacggtcg gaatttgcaa atgcaataaa gccaaatttc cccaataatg ttatattttt atctatacat gctaatgatg ctccaaacgg tgaagctaga ggagttgagt tttggtatct tcctaaggat tcaaaaagag aagttattaa agattttaag ggatatgata ttaaaggtaa tagatactta agcgagctta atgatatact agatattaaa tataaatatg aatcaaaaag attggctgaa attttgtata aagtgtttaa aaatgagtta agcgaaacta atattaggcc aatcagaaaa gagcaatggt ttgtaataaa aaacagcagt atgcctgctg tgttgattga aatggggttt ttatccaata ttttagatgc taaattaatt ttggattata attacatgag caagtttaat attctaatac ttaaagcttt aatggaattt ataaattttt atgaaaaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa