BobuA.11339.a

phnP protein (phnP) (BB_0533)

CENTER ID: BobuA.11339.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.11339.a.B1.GE39124 full length 1 253
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.924
RefSeq: NP_212667.1
UniProt: O51483

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVGTFLGTGA SSGVPMLNCS CKVCTSSYSK NKRLRSSFFL ESSSGIKLLI DTGPDIRQQL LREKIDRLDL VLYTHEHYDH IMGFDDIKFY TRCAPLNIYA RDTAMAHIRN AFPYNFTSKP SLSGKADIIA NVIRDFEPFF FKSLKIVPIP LIHGDIISLG YRVGNLAYLT DVKFIPEASY DYLKNLDLLI IDAMRIKPHP AHLNFSEAFC VIKKINPKIS YFTHIAHDIM HEEFDYLEKD NIYLAYDGLK IYI
NT Sequence
atggttggaa cctttttggg aacgggggct tcaagtggtg ttcctatgtt aaattgtagc tgtaaagtct gtacttcaag ttatagtaaa aataaaagat tgagaagttc tttttttctt gagtcatctt ctggcatcaa attgttgatt gatacaggtc ctgatattag acagcaactt ttaagagaga aaatagatag gcttgattta gtgctttaca cgcatgaaca ttatgatcat attatgggtt ttgatgatat taagttctat acacgatgtg cccctttaaa catttatgct agagatactg ccatggctca cataaggaat gcttttccgt ataattttac atccaaaccc tctttaagtg gaaaggcaga cattattgcc aatgttatta gggattttga gccctttttt tttaaaagtc taaagatagt gccaattcct ttgattcatg gggatataat tagtttaggt tatagggtag gcaatttagc gtatcttact gatgttaaat ttattcccga agcgtcttat gattatttaa aaaatttaga tctactcata atagatgcta tgaggattaa gcctcatcct gcgcatttaa atttttcaga ggctttttgt gtaattaaaa agatcaatcc taaaatttct tactttacac atattgcaca cgatataatg catgaagaat ttgattattt agaaaaagat aatatctatt tagcttatga tggattaaag atttatattt ga
Details for BobuA.11339.a.B1.GE39124
HARVESTED ON: 5/6/2015
SEQUENCED ON: 5/6/2015
EXPECTED MW: 30kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHMVG TFLGTGASSG VPMLNCSCKV CTSSYSKNKR LRSSFFLESS SGIKLLIDTG PDIRQQLLRE KIDRLDLVLY THEHYDHIMG FDDIKFYTRC APLNIYARDT AMAHIRNAFP YNFTSKPSLS GKADIIANVI RDFEPFFFKS LKIVPIPLIH GDIISLGYRV GNLAYLTDVK FIPEASYDYL KNLDLLIIDA MRIKPHPAHL NFSEAFCVIK KINPKISYFT HIAHDIMHEE FDYLEKDNIY LAYDGLKIYI
Validated NT Sequence
atcttactca cttaaatata aatctttaat ccatcataag ctaaatagat attatctttt tctaaataat caaattcttc atgcattata tcgtgtgcaa tatgtgtaaa gtaagaaatt ttaggattga tctttttaat tacacaaaaa gcctctgaaa aatttaaatg cgcaggatga ggcttaatcc tcatagcatc tattatgagt agatctaaat tttttaaata atcataagac gcttcgggaa taaatttaac atcagtaaga tacgctaaat tgcctaccct ataacctaaa ctaattatat ccccatgaat caaaggaatt ggcactatct ttagactttt aaaaaaaaag ggctcaaaat ccctaataac attggcaata atgtctgcct ttccacttaa agagggtttg gatgtaaaat tatacggaaa agcattcctt atgtgagcca tggcagtatc tctagcataa atgtttaaag gggcacatcg tgtatagaac ttaatatcat caaaacccat aatatgatca taatgttcat gcgtgtaaag cactaaatca agcctatcta ttttctctct taaaagttgc tgtctaatat caggacctgt atcaatcaac aatttgatgc cagaagatga ctcaagaaaa aaagaacttc tcaatctttt atttttacta taacttgaag tacagacttt acagctacaa tttaacatag gaacaccact tgaagccccc gttcccaaaa aggttccaac catatggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMV GTFLGTGASS GVPMLNCSCK VCTSSYSKNK RLRSSFFLES SSGIKLLIDT GPDIRQQLLR EKIDRLDLVL YTHEHYDHIM GFDDIKFYTR CAPLNIYARD TAMAHIRNAF PYNFTSKPSL SGKADIIANV IRDFEPFFFK SLKIVPIPLI HGDIISLGYR VGNLAYLTDV KFIPEASYDY LKNLDLLIID AMRIKPHPAH LNFSEAFCVI KKINPKISYF THIAHDIMHE EFDYLEKDNI YLAYDGLKIY I
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggttg gaaccttttt gggaacgggg gcttcaagtg gtgttcctat gttaaattgt agctgtaaag tctgtacttc aagttatagt aaaaataaaa gattgagaag ttcttttttt cttgagtcat cttctggcat caaattgttg attgatacag gtcctgatat tagacagcaa cttttaagag agaaaataga taggcttgat ttagtgcttt acacgcatga acattatgat catattatgg gttttgatga tattaagttc tatacacgat gtgccccttt aaacatttat gctagagata ctgccatggc tcacataagg aatgcttttc cgtataattt tacatccaaa ccctctttaa gtggaaaggc agacattatt gccaatgtta ttagggattt tgagcccttt ttttttaaaa gtctaaagat agtgccaatt cctttgattc atggggatat aattagttta ggttataggg taggcaattt agcgtatctt actgatgtta aatttattcc cgaagcgtct tatgattatt taaaaaattt agatctactc ataatagatg ctatgaggat taagcctcat cctgcgcatt taaatttttc agaggctttt tgtgtaatta aaaagatcaa tcctaaaatt tcttacttta cacatattgc acacgatata atgcatgaag aatttgatta tttagaaaaa gataatatct atttagctta tgatggatta aagatttata tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat