BobuA.10537.a

cell division protein (ftsA) (BB_0300)

CENTER ID: BobuA.10537.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.10537.a.B1.GE39081 full length 1 413
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.699
RefSeq: NP_212434.1
RefSeq: WP_002656287.1
UniProt: Q44774

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRNLIVGLD VGTSKICTVV AEVNLNDQLE IVGIGTSISR GVRKGVLINI EAALDSISNS IEAAELISGC DITSLSVSMS GSSVEGTNSR GVVAINSKTR EINEEDVERV IEAAKAIVIP MDREILHVIP QEFIVDGIPH IKNPIDMMGI RLEGEVHIIT GSSSSSQNLV RCVNRAGFAV DEVVLGSLAS SYATLSKEER EMGVLFIDMG KGTTDIILYI DGSPYYTGVI PIGVNRVTLD IAQVWKVPED VAENIKITAG IAHPSILESQ METVIIPNLG TRPPQEKSRK ELSVIINSRL REIFEMMKAE ILKRGLYNKI NGGIVLTGGG ALFPGISNLI EEVFNYPARI GLPMSINGIG EEHIDPKFSS ALGLVLYKHE QQKFNKLKKV SSKVKRKNKI SSKLKGWFLK EWF
NT Sequence
gtgtctagga atttgatagt aggtttagat gttggaactt caaaaatttg tactgttgtt gccgaggtga atttaaatga tcaattagaa atagttggaa taggcactag tatatcaaga ggagttagga agggagtttt aataaatatt gaggcggctc ttgattcaat atctaattct attgaggctg cagagctcat ctcaggatgt gacattacat cactttcagt ttctatgtct ggaagtagtg ttgaggggac taattcacgc ggtgttgttg caataaattc aaaaacaaga gagattaacg aagaagatgt tgaaagggta atcgaagcgg caaaggcaat tgttattcca atggatagag aaattcttca tgttattcct caagaattta ttgtagatgg aataccccat ataaaaaatc caatagatat gatgggtatt cgtcttgaag gagaggtgca cattattacg ggctctagtt cttctagtca gaatttagtc agatgcgtaa atcgagctgg ctttgccgtt gatgaggttg ttcttggaag tctagcttca tcttatgcaa ctctttctaa agaagagcgt gagatggggg ttttatttat tgatatgggc aaagggacaa cagatattat tctttatatt gatggttctc cttattatac gggtgtaatt cccattggtg ttaatagagt gactcttgat attgcgcaag tttggaaggt tcctgaggat gttgctgaaa atattaaaat aacagctggc attgctcatc cgtctattct tgagagtcaa atggaaactg taattattcc aaatcttgga actcgacccc ctcaagaaaa aagtagaaaa gagttgtctg taataattaa ttcaagactg agagaaattt ttgaaatgat gaaagcggaa atacttaagc gcggacttta taataaaatt aatggtggaa tagttttaac aggcggagga gctttattcc caggcatttc taatttaata gaagaggtat ttaattatcc tgcaagaata ggtttgccaa tgagtattaa tggaattgga gaagagcata tagaccccaa gttttcttca gctcttggtc ttgttcttta taagcacgag caacaaaaat tcaataaatt aaagaaggta agcagtaaag ttaaaagaaa aaataaaata tcttcaaagt tgaaaggttg gtttttgaaa gaatggtttt ga
Details for BobuA.10537.a.B1.GE39081
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV SRNLIVGLDV GTSKICTVVA EVNLNDQLEI VGIGTSISRG VRKGVLINIE AALDSISNSI EAAELISGCD ITSLSVSMSG SSVEGTNSRG VVAINSKTRE INEEDVERVI EAAKAIVIPM DREILHVIPQ EFIVDGIPHI KNPIDMMGIR LEGEVHIITG SSSSSQNLVR CVNRAGFAVD EVVLGSLASS YATLSKEERE MGVLFIDMGK GTTDIILYID GSPYYTGVIP IGVNRVTLDI AQVWKVPEDV AENIKITAGI AHPSILESQM ETVIIPNLGT RPPQEKSRKE LSVIINSRLR EIFEMMKAEI LKRGLYNKIN GGIVLTGGGA LFPGISNLIE EVFNYPARIG LPMSINGIGE EHIDPKFSSA LXXVLYKHEQ QKFNKLKKVS SKVKRKNKIS SXX
Validated NT Sequence
tnnnnnttga agatatttta ttttttcttt taactttact gcttaccttc tttaatttat tgaatttttg ttgctcgtgc ttataaagaa caannncaag agctgaagaa aacttggggt ctatatgctc ttctccaatt ccattaatac tcattggcaa acctattctt gcaggataat taaatacctc ttctattaaa ttagaaatgc ctgggaataa agctcctccg cctgttaaaa ctattccacc attaatttta ttataaagtc cgcgcttaag tatttccgct ttcatcattt caaaaatttc tctcagtctt gaattaatta ttacagacaa ctcttttcta cttttttctt gagggggtcg agttccaaga tttggaataa ttacagtttc catttgactc tcaagaatag acggatgagc aatgccagct gttattttaa tattttcagc aacatcctca ggaaccttcc aaacttgcgc aatatcaaga gtcactctat taacaccaat gggaattaca cccgtataat aaggagaacc atcaatataa agaataatat ctgttgtccc tttgcccata tcaataaata aaacccccat ctcacgctct tctttagaaa gagttgcata agatgaagct agacttccaa gaacaacctc atcaacggca aagccagctc gatttacgca tctgactaaa ttctgactag aagaactaga gcccgtaata atgtgcacct ctccttcaag acgaataccc atcatatcta ttggattttt tatatggggt attccatcta caataaattc ttgaggaata acatgaagaa tttctctatc cattggaata acaattgcct ttgccgcttc gattaccctt tcaacatctt cttcgttaat ctctcttgtt tttgaattta ttgcaacaac accgcgtgaa ttagtcccct caacactact tccagacata gaaactgaaa gtgatgtaat gtcacatcct gagatgagct ctgcagcctc aatagaatta gatattgaat caagagccgc ctcaatattt attaaaactc ccttcctaac tcctcttgat atactagtgc ctattccaac tatttctaat tgatcattta aattcacctc ggcaacaaca gtacaaattt ttgaagttcc aacatctaaa cctactatca aattcctaga caccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMS RNLIVGLDVG TSKICTVVAE VNLNDQLEIV GIGTSISRGV RKGVLINIEA ALDSISNSIE AAELISGCDI TSLSVSMSGS SVEGTNSRGV VAINSKTREI NEEDVERVIE AAKAIVIPMD REILHVIPQE FIVDGIPHIK NPIDMMGIRL EGEVHIITGS SSSSQNLVRC VNRAGFAVDE VVLGSLASSY ATLSKEEREM GVLFIDMGKG TTDIILYIDG SPYYTGVIPI GVNRVTLDIA QVWKVPEDVA ENIKITAGIA HPSILESQME TVIIPNLGTR PPQEKSRKEL SVIINSRLRE IFEMMKAEIL KRGLYNKING GIVLTGGGAL FPGISNLIEE VFNYPARIGL PMSINGIGEE HIDPKFSSAL GLVLYKHEQQ KFNKLKKVSS KVKRKNKISS KLKGWFLKEW F
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtcta ggaatttgat agtaggttta gatgttggaa cttcaaaaat ttgtactgtt gttgccgagg tgaatttaaa tgatcaatta gaaatagttg gaataggcac tagtatatca agaggagtta ggaagggagt tttaataaat attgaggcgg ctcttgattc aatatctaat tctattgagg ctgcagagct catctcagga tgtgacatta catcactttc agtttctatg tctggaagta gtgttgaggg gactaattca cgcggtgttg ttgcaataaa ttcaaaaaca agagagatta acgaagaaga tgttgaaagg gtaatcgaag cggcaaaggc aattgttatt ccaatggata gagaaattct tcatgttatt cctcaagaat ttattgtaga tggaataccc catataaaaa atccaataga tatgatgggt attcgtcttg aaggagaggt gcacattatt acgggctcta gttcttctag tcagaattta gtcagatgcg taaatcgagc tggctttgcc gttgatgagg ttgttcttgg aagtctagct tcatcttatg caactctttc taaagaagag cgtgagatgg gggttttatt tattgatatg ggcaaaggga caacagatat tattctttat attgatggtt ctccttatta tacgggtgta attcccattg gtgttaatag agtgactctt gatattgcgc aagtttggaa ggttcctgag gatgttgctg aaaatattaa aataacagct ggcattgctc atccgtctat tcttgagagt caaatggaaa ctgtaattat tccaaatctt ggaactcgac cccctcaaga aaaaagtaga aaagagttgt ctgtaataat taattcaaga ctgagagaaa tttttgaaat gatgaaagcg gaaatactta agcgcggact ttataataaa attaatggtg gaatagtttt aacaggcgga ggagctttat tcccaggcat ttctaattta atagaagagg tatttaatta tcctgcaaga ataggtttgc caatgagtat taatggaatt ggagaagagc atatagaccc caagttttct tcagctcttg gtcttgttct ttataagcac gagcaacaaa aattcaataa attaaagaag gtaagcagta aagttaaaag aaaaaataaa atatcttcaa agttgaaagg ttggtttttg aaagaatggt tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat