BobuA.10100.a

periplasmic serine protease DO (htrA) (BB_0104)

CENTER ID: BobuA.10100.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.10100.a.B2.GE39080 mature protein 37 474
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.10100.a.B2.PS02468 mature protein 37 474

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.502
RefSeq: NP_212238.2
UniProt: O51131

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKKFFSGFL LSFLALSIGF FIGMHYLASN RSNIVFAEEK DNTVRALQDS FREVSKKILP SSVEVHATGV IKQSFPIPFF FFDMPEFDSE RKSNWAGSGV IIGRDSQKKS LFYVVTNSHV VDKATELEVV SYDKKKHKAK LIGKDEKKDI ALISFESDDA TIKVADLGDS DKLEIGDWVM AVGSPFQFSF TVTAGIVSGL QRSANPNLQS RNLFIQTDAA INRGNSGGPL VNIKGEVIGI NAWIASNSGG NIGLGFAIPV NNIKSTVDFF LKGKKIESAW LGISFYPLKT RDSEVLKSLG VESNDVSAAI IASLYPGSPA VKSGLRAGDI IMKVNGVSMS VFQDVTSYIS DFYAGEKVNV EILRGNVKKN IEIVLAVRPK DKELSSSKML PGFVVYPLVE DIKAQLNLRN WIKGVVVDYI DKNLASNIKM KSGDVILSVN SKSVSNLREF YDALEVGKNT YKILRGNDSF KITF
NT Sequence
gtggaaaaaa agtttttttc tggatttctt cttagttttt tggctttgag tattggattt tttattggga tgcattattt agcttctaat agaagcaaca ttgtttttgc agaagaaaag gacaataccg tacgagcctt acaagattct tttagagagg tttccaagaa aattttacca tcatctgtgg aagttcatgc aacaggggta atcaagcaga gttttcctat tccatttttc ttttttgata tgccagaatt tgattctgag agaaaaagca attgggcggg gtctggagta ataattggta gagattctca aaaaaaatca ttattttatg tggttacgaa tagtcatgtg gtagataagg caactgaact tgaagttgta tcttatgata aaaaaaagca caaggctaag ttaattggca aagatgaaaa aaaggatatt gcccttatta gctttgaaag tgatgacgca actattaaag tagctgatct tggagatagt gataagcttg aaataggtga ttgggttatg gcagtgggca gcccttttca atttagtttt acagttacag caggtattgt aagtggattg caacgttctg caaatcctaa tttgcaatca aggaatctgt ttattcaaac cgatgctgca atcaacaggg gtaattcagg tggtcctctt gtaaatataa agggcgaggt tattggaatt aatgcttgga tagcttcaaa ttctggcgga aatattgggt tgggttttgc aattcctgtt aacaatatta aaagcactgt agattttttc cttaaaggta aaaaaattga atcggcttgg cttggaattt ctttttatcc gctaaagaca agagattctg aggtgctaaa aagcttaggg gttgaaagta atgatgtttc agctgcaatt attgcttctc tttatccggg ttcacccgct gttaaatcag ggcttagggc aggggatatt attatgaagg ttaatggggt ttccatgagc gtttttcaag atgttacatc atatattagt gatttttatg ctggtgagaa agtaaatgta gaaatcttaa gaggcaatgt caaaaaaaat attgagattg ttcttgctgt tagacctaag gataaagagc tttcttcttc aaagatgctt ccaggctttg ttgtgtatcc attggttgag gatattaaag ctcagcttaa tttaagaaat tggattaaag gtgttgttgt tgattatatt gataaaaatt tagcatcaaa tattaaaatg aaatcaggag atgtaattct ttctgtaaat tcaaaaagtg tttctaattt aagagaattt tatgatgctc ttgaggttgg aaaaaatact tataaaattt tgagaggaaa cgattctttt aaaattacat tttag
Details for BobuA.10100.a.B2.GE39080
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA EEKDNTVRAL QDSFREVSKK ILPSSVEVHA TGVIKQSFPI PFFFFDMPEF DSERKSNWAG SGVIIGRDSQ KKSLFYVVTN SHVVDKATEL EVVSYDKKKH KAKLIGKDEK KDIALISFES DDATIKVADL GDSDKLEIGD WVMAVGSPFQ FSFTVTAGIV SGLQRSANPN LQSRNLFIQT DAAINRGNSG GPLVNIKGEV IGINAWIASN SGGNIGLGFA IPVNNIKSTV DFFLKGKKIE SAWLGISFYP LKTRDSEVLK SLGVESNDVS AAIIASLYPG SPAVKSGLRA GDIIMKVNGV SMSVFQDVTS YISDFYAGEK VNVEILRGNV KKNIEIVLAV RPKDKELSSS KMLPGFVVYP LVEDIKAQLN LRNWIKGVVV DYIDKNLASN IKMKSGDVIL SVNSKSVSNL REFYDALEVG KNTYKILRGN
Validated NT Sequence
tcgtttcctc tcaaaatttt ataagtattt tttccaacct caagagcatc ataaaattct cttaaattag aaacactttt tgaatttaca gaaagaatta catctcctga tttcatttta atatttgatg ctaaattttt atcaatataa tcaacaacaa cacctttaat ccaatttctt aaattaagct gagctttaat atcctcaacc aatggataca caacaaagcc tggaagcatc tttgaagaag aaagctcttt atccttaggt ctaacagcaa gaacaatctc aatatttttt ttgacattgc ctcttaagat ttctacattt actttctcac cagcataaaa atcactaata tatgatgtaa catcttgaaa aacgctcatg gaaaccccat taaccttcat aataatatcc cctgccctaa gccctgattt aacagcgggt gaacccggat aaagagaagc aataattgca gctgaaacat cattactttc aacccctaag ctttttagca cctcagaatc tcttgtcttt agcggataaa aagaaattcc aagccaagcc gattcaattt ttttaccttt aaggaaaaaa tctacagtgc ttttaatatt gttaacagga attgcaaaac ccaacccaat atttccgcca gaatttgaag ctatccaagc attaattcca ataacctcgc cctttatatt tacaagagga ccacctgaat tacccctgtt gattgcagca tcggtttgaa taaacagatt ccttgattgc aaattaggat ttgcagaacg ttgcaatcca cttacaatac ctgctgtaac tgtaaaacta aattgaaaag ggctgcccac tgccataacc caatcaccta tttcaagctt atcactatct ccaagatcag ctactttaat agttgcgtca tcactttcaa agctaataag ggcaatatcc tttttttcat ctttgccaat taacttagcc ttgtgctttt ttttatcata agatacaact tcaagttcag ttgccttatc taccacatga ctattcgtaa ccacataaaa taatgatttt ttttgagaat ctctaccaat tattactcca gaccccgccc aattgctttt tctctcagaa tcaaattctg gcatatcaaa aaagaaaaat ggaataggaa aactctgctt gattacccct gttgcatgaa cttccacaga tgatggtaaa attttcttgg aaacctctct aaaagaatct tgtaaggctc gtacggtatt gtccttttct tctgccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHAE EKDNTVRALQ DSFREVSKKI LPSSVEVHAT GVIKQSFPIP FFFFDMPEFD SERKSNWAGS GVIIGRDSQK KSLFYVVTNS HVVDKATELE VVSYDKKKHK AKLIGKDEKK DIALISFESD DATIKVADLG DSDKLEIGDW VMAVGSPFQF SFTVTAGIVS GLQRSANPNL QSRNLFIQTD AAINRGNSGG PLVNIKGEVI GINAWIASNS GGNIGLGFAI PVNNIKSTVD FFLKGKKIES AWLGISFYPL KTRDSEVLKS LGVESNDVSA AIIASLYPGS PAVKSGLRAG DIIMKVNGVS MSVFQDVTSY ISDFYAGEKV NVEILRGNVK KNIEIVLAVR PKDKELSSSK MLPGFVVYPL VEDIKAQLNL RNWIKGVVVD YIDKNLASNI KMKSGDVILS VNSKSVSNLR EFYDALEVGK NTYKILRGND SFKITF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcagaag aaaaggacaa taccgtacga gccttacaag attcttttag agaggtttcc aagaaaattt taccatcatc tgtggaagtt catgcaacag gggtaatcaa gcagagtttt cctattccat ttttcttttt tgatatgcca gaatttgatt ctgagagaaa aagcaattgg gcggggtctg gagtaataat tggtagagat tctcaaaaaa aatcattatt ttatgtggtt acgaatagtc atgtggtaga taaggcaact gaacttgaag ttgtatctta tgataaaaaa aagcacaagg ctaagttaat tggcaaagat gaaaaaaagg atattgccct tattagcttt gaaagtgatg acgcaactat taaagtagct gatcttggag atagtgataa gcttgaaata ggtgattggg ttatggcagt gggcagccct tttcaattta gttttacagt tacagcaggt attgtaagtg gattgcaacg ttctgcaaat cctaatttgc aatcaaggaa tctgtttatt caaaccgatg ctgcaatcaa caggggtaat tcaggtggtc ctcttgtaaa tataaagggc gaggttattg gaattaatgc ttggatagct tcaaattctg gcggaaatat tgggttgggt tttgcaattc ctgttaacaa tattaaaagc actgtagatt ttttccttaa aggtaaaaaa attgaatcgg cttggcttgg aatttctttt tatccgctaa agacaagaga ttctgaggtg ctaaaaagct taggggttga aagtaatgat gtttcagctg caattattgc ttctctttat ccgggttcac ccgctgttaa atcagggctt agggcagggg atattattat gaaggttaat ggggtttcca tgagcgtttt tcaagatgtt acatcatata ttagtgattt ttatgctggt gagaaagtaa atgtagaaat cttaagaggc aatgtcaaaa aaaatattga gattgttctt gctgttagac ctaaggataa agagctttct tcttcaaaga tgcttccagg ctttgttgtg tatccattgg ttgaggatat taaagctcag cttaatttaa gaaattggat taaaggtgtt gttgttgatt atattgataa aaatttagca tcaaatatta aaatgaaatc aggagatgta attctttctg taaattcaaa aagtgtttct aatttaagag aattttatga tgctcttgag gttggaaaaa atacttataa aattttgaga ggaaacgatt cttttaaaat tacattttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BobuA.10100.a.B2.PS02468
PURIFICATION DATe: 8/5/2015
CONCENTRATION: 13.59mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA EEKDNTVRAL QDSFREVSKK ILPSSVEVHA TGVIKQSFPI PFFFFDMPEF DSERKSNWAG SGVIIGRDSQ KKSLFYVVTN SHVVDKATEL EVVSYDKKKH KAKLIGKDEK KDIALISFES DDATIKVADL GDSDKLEIGD WVMAVGSPFQ FSFTVTAGIV SGLQRSANPN LQSRNLFIQT DAAINRGNSG GPLVNIKGEV IGINAWIASN SGGNIGLGFA IPVNNIKSTV DFFLKGKKIE SAWLGISFYP LKTRDSEVLK SLGVESNDVS AAIIASLYPG SPAVKSGLRA GDIIMKVNGV SMSVFQDVTS YISDFYAGEK VNVEILRGNV KKNIEIVLAV RPKDKELSSS KMLPGFVVYP LVEDIKAQLN LRNWIKGVVV DYIDKNLASN IKMKSGDVIL SVNSKSVSNL REFYDALEVG KNTYKILRGN
Validated NT Sequence
tcgtttcctc tcaaaatttt ataagtattt tttccaacct caagagcatc ataaaattct cttaaattag aaacactttt tgaatttaca gaaagaatta catctcctga tttcatttta atatttgatg ctaaattttt atcaatataa tcaacaacaa cacctttaat ccaatttctt aaattaagct gagctttaat atcctcaacc aatggataca caacaaagcc tggaagcatc tttgaagaag aaagctcttt atccttaggt ctaacagcaa gaacaatctc aatatttttt ttgacattgc ctcttaagat ttctacattt actttctcac cagcataaaa atcactaata tatgatgtaa catcttgaaa aacgctcatg gaaaccccat taaccttcat aataatatcc cctgccctaa gccctgattt aacagcgggt gaacccggat aaagagaagc aataattgca gctgaaacat cattactttc aacccctaag ctttttagca cctcagaatc tcttgtcttt agcggataaa aagaaattcc aagccaagcc gattcaattt ttttaccttt aaggaaaaaa tctacagtgc ttttaatatt gttaacagga attgcaaaac ccaacccaat atttccgcca gaatttgaag ctatccaagc attaattcca ataacctcgc cctttatatt tacaagagga ccacctgaat tacccctgtt gattgcagca tcggtttgaa taaacagatt ccttgattgc aaattaggat ttgcagaacg ttgcaatcca cttacaatac ctgctgtaac tgtaaaacta aattgaaaag ggctgcccac tgccataacc caatcaccta tttcaagctt atcactatct ccaagatcag ctactttaat agttgcgtca tcactttcaa agctaataag ggcaatatcc tttttttcat ctttgccaat taacttagcc ttgtgctttt ttttatcata agatacaact tcaagttcag ttgccttatc taccacatga ctattcgtaa ccacataaaa taatgatttt ttttgagaat ctctaccaat tattactcca gaccccgccc aattgctttt tctctcagaa tcaaattctg gcatatcaaa aaagaaaaat ggaataggaa aactctgctt gattacccct gttgcatgaa cttccacaga tgatggtaaa attttcttgg aaacctctct aaaagaatct tgtaaggctc gtacggtatt gtccttttct tctgccatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHAE EKDNTVRALQ DSFREVSKKI LPSSVEVHAT GVIKQSFPIP FFFFDMPEFD SERKSNWAGS GVIIGRDSQK KSLFYVVTNS HVVDKATELE VVSYDKKKHK AKLIGKDEKK DIALISFESD DATIKVADLG DSDKLEIGDW VMAVGSPFQF SFTVTAGIVS GLQRSANPNL QSRNLFIQTD AAINRGNSGG PLVNIKGEVI GINAWIASNS GGNIGLGFAI PVNNIKSTVD FFLKGKKIES AWLGISFYPL KTRDSEVLKS LGVESNDVSA AIIASLYPGS PAVKSGLRAG DIIMKVNGVS MSVFQDVTSY ISDFYAGEKV NVEILRGNVK KNIEIVLAVR PKDKELSSSK MLPGFVVYPL VEDIKAQLNL RNWIKGVVVD YIDKNLASNI KMKSGDVILS VNSKSVSNLR EFYDALEVGK NTYKILRGND SFKITF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCAGAAG AAAAGGACAA TACCGTACGA GCCTTACAAG ATTCTTTTAG AGAGGTTTCC AAGAAAATTT TACCATCATC TGTGGAAGTT CATGCAACAG GGGTAATCAA GCAGAGTTTT CCTATTCCAT TTTTCTTTTT TGATATGCCA GAATTTGATT CTGAGAGAAA AAGCAATTGG GCGGGGTCTG GAGTAATAAT TGGTAGAGAT TCTCAAAAAA AATCATTATT TTATGTGGTT ACGAATAGTC ATGTGGTAGA TAAGGCAACT GAACTTGAAG TTGTATCTTA TGATAAAAAA AAGCACAAGG CTAAGTTAAT TGGCAAAGAT GAAAAAAAGG ATATTGCCCT TATTAGCTTT GAAAGTGATG ACGCAACTAT TAAAGTAGCT GATCTTGGAG ATAGTGATAA GCTTGAAATA GGTGATTGGG TTATGGCAGT GGGCAGCCCT TTTCAATTTA GTTTTACAGT TACAGCAGGT ATTGTAAGTG GATTGCAACG TTCTGCAAAT CCTAATTTGC AATCAAGGAA TCTGTTTATT CAAACCGATG CTGCAATCAA CAGGGGTAAT TCAGGTGGTC CTCTTGTAAA TATAAAGGGC GAGGTTATTG GAATTAATGC TTGGATAGCT TCAAATTCTG GCGGAAATAT TGGGTTGGGT TTTGCAATTC CTGTTAACAA TATTAAAAGC ACTGTAGATT TTTTCCTTAA AGGTAAAAAA ATTGAATCGG CTTGGCTTGG AATTTCTTTT TATCCGCTAA AGACAAGAGA TTCTGAGGTG CTAAAAAGCT TAGGGGTTGA AAGTAATGAT GTTTCAGCTG CAATTATTGC TTCTCTTTAT CCGGGTTCAC CCGCTGTTAA ATCAGGGCTT AGGGCAGGGG ATATTATTAT GAAGGTTAAT GGGGTTTCCA TGAGCGTTTT TCAAGATGTT ACATCATATA TTAGTGATTT TTATGCTGGT GAGAAAGTAA ATGTAGAAAT CTTAAGAGGC AATGTCAAAA AAAATATTGA GATTGTTCTT GCTGTTAGAC CTAAGGATAA AGAGCTTTCT TCTTCAAAGA TGCTTCCAGG CTTTGTTGTG TATCCATTGG TTGAGGATAT TAAAGCTCAG CTTAATTTAA GAAATTGGAT TAAAGGTGTT GTTGTTGATT ATATTGATAA AAATTTAGCA TCAAATATTA AAATGAAATC AGGAGATGTA ATTCTTTCTG TAAATTCAAA AAGTGTTTCT AATTTAAGAG AATTTTATGA TGCTCTTGAG GTTGGAAAAA ATACTTATAA AATTTTGAGA GGAAACGATT CTTTTAAAAT TACATTTTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT