BobuA.10014.b

ribose-galactose ABC transporter,ATP-binding protein (mglA) (BB_0677)

CENTER ID: BobuA.10014.b
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.10014.b.B1.GE39079 full length 1 536
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1068
RefSeq: NP_212811.1
UniProt: O51620

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKEDVLVLEN ITKKYGDFVA NDNVSIKFKA GEVHAILGEN GAGKTTLMKT IYGIHQVNSG RIILKGQEIN FKDSSEAIRN GIGMVFQHFM LIPQFTAVQN IILGYENSKF GFLDYKQARK KISSLSEKYG LKIDLEKRVE DLSVGMEQKI EILKVLYRNA DIIIFDEPTA VLAPSEVDDF INILKVLAQE GHTVILITHK IKEIRSIAKK CTIMRLGKVV KTVNIADIDD KDLTKLMIGK EVALRSSKIK FENHFNILEI KNLSVKDERG VLKVKDVNLD LRNGEILGIS GIEGSGQEDL VDAILGLKSI FKGDILKKNS SGNLESLKGL TIKQRIDKKI GNIPSDRQKH GLILEFNVMQ NIGLKSFDNP DYLRLKTIHL KSNFDLKFNF FNFIKRQFNK FKKQFVGFDL NILRKLSNQL VSHFDIRPRD ILSKVKHLSG GNQQKVIVAR EISLEPDILL AIQPTRGLDV GAVENIYKRI IEQRDAGRSV LLVSLELDEL VNVCDRIAVM HGGRIVGILE DNFDIDVIGK MMIGLS
NT Sequence
atgaaagaag atgtactagt attagaaaat attacaaaaa agtatggtga ttttgttgcc aatgataatg tttctattaa atttaaggca ggcgaagttc atgctattct tggagaaaat ggtgctggaa agactacctt aatgaaaact atttatggga ttcatcaagt aaatagtggt cgaattattt taaaaggtca agaaataaac tttaaggatt caagcgaagc tattcgaaat gggattggaa tggttttcca acactttatg ttaattccgc aatttactgc tgttcaaaac attattttgg gatatgaaaa ttcaaaattt ggttttcttg attacaaaca agctagaaaa aagataagtt ctctttcgga aaagtatggt ttaaagatag atttagaaaa aagggttgaa gacttgagtg ttggcatgga acaaaaaata gagatattga aagttcttta tcgaaatgca gatattatta tttttgatga acctacagca gtgcttgctc caagtgaagt tgatgatttt ataaatattt taaaggtact cgcccaagag ggtcatactg taatacttat tacccataaa ataaaagaaa ttagatctat tgcaaagaaa tgtacaatta tgcgtcttgg gaaggttgta aaaactgtta atattgctga tattgatgac aaagatctta caaaattaat gataggaaaa gaagttgcac ttcgctcatc taaaattaaa tttgaaaatc attttaatat tcttgaaata aagaatttaa gtgttaaaga tgagagagga gttttaaaag ttaaagacgt taatcttgat ctgagaaatg gagaaattct tggaatatca ggtattgagg ggagtggtca ggaggattta gttgatgcaa ttttgggttt gaaaagcata tttaagggcg atattttgaa aaaaaattct tcggggaatt tggaatcttt aaaaggttta actattaaac aaagaataga taaaaaaatt ggcaatattc cttcggacag gcaaaaacac ggccttattt tagaattcaa tgttatgcaa aatattggac ttaagagctt tgataatcct gattatttga gattaaaaac aattcatttg aagagtaatt ttgatttaaa attcaatttt ttcaatttta ttaaaagaca attcaataag tttaaaaaac aatttgtggg gtttgatctt aatattttaa gaaaattgag caatcaactt gtaagtcatt ttgatattag accaagagat attttaagca aggtaaagca tttatccgga ggtaatcaac aaaaagttat tgttgctcgt gagattagtt tagagccaga tattcttttg gctattcagc ctacaagagg ccttgatgtt ggagctgttg agaatattta caaaagaata atagagcaaa gagatgcggg tagatctgtt ttattggttt ctcttgaact tgatgagctt gttaatgttt gtgacaggat agctgtaatg catggtggaa gaatagtggg cattttagag gataattttg atattgatgt tattggtaaa atgatgatag gcttaagcta a
Details for BobuA.10014.b.B1.GE39079
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 61kDa
OBSERVED MW: 61kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMK EDVLVLENIT KKYGDFVAND NVSIKFKAGE VHAILGENGA GKTTLMKTIY GIHQVNSGRI ILKGQEINFK DSSEAIRNGI GMVFQHFMLI PQFTAVQNII LGYENSKFGF LDYKQARKKI SSLSEKYGLK IDLEKRVEDL SVGMEQKIEI LKVLYRNADI IIFDEPTAVL APSEVDDFIN ILKVLAQEGH TVILITHKIK EIRSIAKKCT IMRLGKVVKT VNIADIDDKD LTKLMIGKEV ALRSSKIKFE NHXXILEIKN LSVKDERGVL KVKDVNLDLR NGEILGISGI EGSGQEDLVD AILGLKSIFK GDILKKNSSG NLESLKGLTI KQRIDKKIGN IPSDRQKHGL ILEFNVMQNI GLKSFDNPDY LRLKTIHLKS NFDLKFNFFN FIKRQFNKFK KQFVGFDLNI LRKLSNQLVS HFDIRPRDIL SKVKHLSGGN QQKVIVAREI SLEPDILLAI QPTRGLDVGA VENIYKRIIE QRDAGRSVLL VSLELDELVN VCDRIAVMHG GRIVGILEDN FDIDVIG
Validated NT Sequence
taccaataac atcaatatca aaattatcct ctaaaatgcc cactattctt ccaccatgca ttacagctat cctgtcacaa acattaacaa gctcatcaag ttcaagagaa accaataaaa cagatctacc cgcatctctt tgctctatta ttcttttgta aatattctca acagctccaa catcaaggcc tcttgtaggc tgaatagcca aaagaatatc tggctctaaa ctaatctcac gagcaacaat aactttttgt tgattacctc cggataaatg ctttaccttg cttaaaatat ctcttggtct aatatcaaaa tgacttacaa gttgattgct caattttctt aaaatattaa gatcaaaccc cacaaattgt tttttaaact tattgaattg tcttttaata aaattgaaaa aattgaattt taaatcaaaa ttactcttca aatgaattgt ttttaatctc aaataatcag gattatcaaa gctcttaagt ccaatatttt gcataacatt gaattctaaa ataaggccgt gtttttgcct gtccgaagga atattgccaa tttttttatc tattctttgt ttaatagtta aaccttttaa agattccaaa ttccccgaag aatttttttt caaaatatcg cccttaaata tgcttttcaa acccaaaatt gcatcaacta aatcctcctg accactcccc tcaatacctg atattccaag aatttctcca tttctcagat caagattaac gtctttaact tttaaaactc ctctctcatc tttaacactt aaattcttta tttcaagaat atnnnnatga ttttcaaatt taattttaga tgagcgaagt gcaacttctt ttcctatcat taattttgta agatctttgt catcaatatc agcaatatta acagttttta caaccttccc aagacgcata attgtacatt tctttgcaat agatctaatt tcttttattt tatgggtaat aagtattaca gtatgaccct cttgggcgag tacctttaaa atatttataa aatcatcaac ttcacttgga gcaagcactg ctgtaggttc atcaaaaata ataatatctg catttcgata aagaactttc aatatctcta ttttttgttc catgccaaca ctcaagtctt caaccctttt ttctaaatct atctttaaac catacttttc cgaaagagaa cttatctttt ttctagcttg tttgtaatca agaaaaccaa attttgaatt ttcatatccc aaaataatgt tttgaacagc agtaaattgc ggaattaaca taaagtgttg gaaaaccatt ccaatcccat ttcgaatagc ttcgcttgaa tccttaaagt ttatttcttg accttttaaa ataattcgac cactatttac ttgatgaatc ccataaatag ttttcattaa ggtagtcttt ccagcaccat tttctccaag aatagcatga acttcgcctg ccttaaattt aatagaaaca ttatcattgg caacaaaatc accatacttt tttgtaatat tttctaatac tagtacatct tctttcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMK EDVLVLENIT KKYGDFVAND NVSIKFKAGE VHAILGENGA GKTTLMKTIY GIHQVNSGRI ILKGQEINFK DSSEAIRNGI GMVFQHFMLI PQFTAVQNII LGYENSKFGF LDYKQARKKI SSLSEKYGLK IDLEKRVEDL SVGMEQKIEI LKVLYRNADI IIFDEPTAVL APSEVDDFIN ILKVLAQEGH TVILITHKIK EIRSIAKKCT IMRLGKVVKT VNIADIDDKD LTKLMIGKEV ALRSSKIKFE NHFNILEIKN LSVKDERGVL KVKDVNLDLR NGEILGISGI EGSGQEDLVD AILGLKSIFK GDILKKNSSG NLESLKGLTI KQRIDKKIGN IPSDRQKHGL ILEFNVMQNI GLKSFDNPDY LRLKTIHLKS NFDLKFNFFN FIKRQFNKFK KQFVGFDLNI LRKLSNQLVS HFDIRPRDIL SKVKHLSGGN QQKVIVAREI SLEPDILLAI QPTRGLDVGA VENIYKRIIE QRDAGRSVLL VSLELDELVN VCDRIAVMHG GRIVGILEDN FDIDVIGKMM IGLS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag aagatgtact agtattagaa aatattacaa aaaagtatgg tgattttgtt gccaatgata atgtttctat taaatttaag gcaggcgaag ttcatgctat tcttggagaa aatggtgctg gaaagactac cttaatgaaa actatttatg ggattcatca agtaaatagt ggtcgaatta ttttaaaagg tcaagaaata aactttaagg attcaagcga agctattcga aatgggattg gaatggtttt ccaacacttt atgttaattc cgcaatttac tgctgttcaa aacattattt tgggatatga aaattcaaaa tttggttttc ttgattacaa acaagctaga aaaaagataa gttctctttc ggaaaagtat ggtttaaaga tagatttaga aaaaagggtt gaagacttga gtgttggcat ggaacaaaaa atagagatat tgaaagttct ttatcgaaat gcagatatta ttatttttga tgaacctaca gcagtgcttg ctccaagtga agttgatgat tttataaata ttttaaaggt actcgcccaa gagggtcata ctgtaatact tattacccat aaaataaaag aaattagatc tattgcaaag aaatgtacaa ttatgcgtct tgggaaggtt gtaaaaactg ttaatattgc tgatattgat gacaaagatc ttacaaaatt aatgatagga aaagaagttg cacttcgctc atctaaaatt aaatttgaaa atcattttaa tattcttgaa ataaagaatt taagtgttaa agatgagaga ggagttttaa aagttaaaga cgttaatctt gatctgagaa atggagaaat tcttggaata tcaggtattg aggggagtgg tcaggaggat ttagttgatg caattttggg tttgaaaagc atatttaagg gcgatatttt gaaaaaaaat tcttcgggga atttggaatc tttaaaaggt ttaactatta aacaaagaat agataaaaaa attggcaata ttccttcgga caggcaaaaa cacggcctta ttttagaatt caatgttatg caaaatattg gacttaagag ctttgataat cctgattatt tgagattaaa aacaattcat ttgaagagta attttgattt aaaattcaat tttttcaatt ttattaaaag acaattcaat aagtttaaaa aacaatttgt ggggtttgat cttaatattt taagaaaatt gagcaatcaa cttgtaagtc attttgatat tagaccaaga gatattttaa gcaaggtaaa gcatttatcc ggaggtaatc aacaaaaagt tattgttgct cgtgagatta gtttagagcc agatattctt ttggctattc agcctacaag aggccttgat gttggagctg ttgagaatat ttacaaaaga ataatagagc aaagagatgc gggtagatct gttttattgg tttctcttga acttgatgag cttgttaatg tttgtgacag gatagctgta atgcatggtg gaagaatagt gggcatttta gaggataatt ttgatattga tgttattggt aaaatgatga taggcttaag ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat