BobuA.01649.a

Hemolysin, putative (BB_0202)

CENTER ID: BobuA.01649.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01649.a.B2.GE39076 soluble domain 140 412
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.599
RefSeq: NP_212336.1
UniProt: O51220

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLELIIILLF IILSAIFSAS ETAYTSLSII QIQDIRKKGK SGISVYNLVQ SPSKLITTIL IGNNISNIVA STLTTKFVLE KYGNSALAIS TGLITIIVLI FAEILPKQIA ILNNEIIALS TSFFLKPLIF IFTPLIYIIN KIIKKILNLF KVKTSSQMTK ESIKNMLSLA GSLGILKNDS RIFMQKMLDI DQVRASEIMT HRTGVFSLSS SSKLKDVIKL IKEEGYSRIP IYKGQSREQI IGILIAKDLI EVNKKDMNKN VSQFIKPAVF VQQNKRIKDI LDIMRKKQKI MAIVIDEYGG FSGILTIEDI VEKIFGAISD EYDIKEEKPL ITQINDNTYS ILGETTFDEI EEAIGISIKH KEYTNTIGGY LIDLLDKIPT KNETVKTNDG EYFIKEIQNN KIETITFIKS KK
NT Sequence
atgctagaat taatcataat actactattt ataatattat ctgctatttt ctcagcatct gaaacagctt atacttcatt aagcataatt cagatacaag acataagaaa aaaggggaaa tcgggaatat ccgtgtacaa tttggttcaa tctccttcaa aattaattac tactattctt atcggcaaca atatatcaaa cattgtagca agcacactta caacaaaatt tgtacttgaa aaatacggaa acagcgcact tgcaatatca acgggcctaa taacaataat cgttctaatt tttgccgaaa tacttccaaa acaaattgca attttaaata atgaaataat tgccctatct acttcatttt ttttaaagcc attaattttt atatttactc cactaatata tataataaac aaaatcataa aaaaaatatt aaatctcttt aaagttaaga ctagctccca aatgactaaa gagagcataa aaaatatgct ctctttagca ggaagtttag gaattttaaa aaatgactct agaatattta tgcaaaaaat gttagatata gatcaagtaa gagcttctga aatcatgacc cacagaacag gggttttttc actctcaagc tcatcaaagc taaaagatgt aatcaaattg atcaaagaag aaggatactc tagaattcct atatacaaag gacaaagcag agaacagata attgggattt taatagccaa agacctcata gaagtcaata aaaaagatat gaataaaaac gtttctcaat ttattaaacc agctgtattt gtacaacaaa acaaaaggat aaaagacata ctagatatta tgaggaaaaa acaaaaaata atggcaatcg taatcgatga atatggtggt ttttcaggga tacttacaat agaagacata gtagaaaaaa tttttggagc aatatctgac gaatacgaca ttaaagagga aaagcctctg attactcaaa tcaacgacaa tacttactca atacttggag aaactacttt tgacgagatt gaagaggcaa ttgggatatc cattaaacat aaagaatata caaatacaat tggtggatac ttaatagatc tgcttgacaa aataccaaca aaaaacgaaa ccgtaaaaac aaatgatgga gaatatttta ttaaggaaat tcagaataat aaaattgaaa caataacttt tatcaaatct aaaaagtag
Details for BobuA.01649.a.B2.GE39076
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN KIIKKILNLF KVKTSSQRTK ESIKNMLSLA GSLGILKNDS RIFMQKMLDI DQVRASEIMT HRTGVFSLSS SSKLKDVIKL IKEEGYSRIP IYKGQSREQI IGILIAKDLI EVNKKDMNKN VSQFIKPAVF VQQNKRIKDI LDIMRKKQKI MAIVIDEYGG FSGILTIEDI VEKIFGAISD EYDIKEEKPL ITQINDNTYS ILGETTFDEI EEAIGISIKH KEYTNTIGGY LIDLLDKIPT KNETVKTNDG EYFIKEIQNN KIEIITFIKS KK
Validated NT Sequence
atcttactca cttacttttt agatttgata aaagttatta tttcaatttt attattctga atttccttaa taaaatattc tccatcattt gtttttacgg tttcgttttt tgttggtatt ttgtcaagca gatctattaa gtatccacca attgtatttg tatattcttt atgtttaatg gatatcccaa ttgcctcttc aatctcgtca aaagtagttt ctccaagtat tgagtaagta ttgtcgttga tttgagtaat cagaggcttt tcctctttaa tgtcgtattc gtcagatatt gctccaaaaa ttttttctac tatgtcttct attgtaagta tccctgaaaa accaccatat tcatcgatta cgattgccat tattttttgt tttttcctca taatatctag tatgtctttt atccttttgt tttgttgtac aaatacagct ggtttaataa attgagaaac gtttttattc atatcttttt tattgacttc tatgaggtct ttggctatta aaatcccaat tatctgttct ctgctttgtc ctttgtatat aggaattcta gagtatcctt cttctttgat caatttgatt acatctttta gctttgatga gcttgagagt gaaaaaaccc ctgttctgtg ggtcatgatt tcagaagctc ttacttgatc tatatctaac attttttgca taaatattct agagtcattt tttaaaattc ctaaacttcc tgctaaagag agcatatttt ttatgctctc tttagtcctt tgggagctag tcttaacttt aaagagattt aatatttttt ttatgatttt gttcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHNK IIKKILNLFK VKTSSQMTKE SIKNMLSLAG SLGILKNDSR IFMQKMLDID QVRASEIMTH RTGVFSLSSS SKLKDVIKLI KEEGYSRIPI YKGQSREQII GILIAKDLIE VNKKDMNKNV SQFIKPAVFV QQNKRIKDIL DIMRKKQKIM AIVIDEYGGF SGILTIEDIV EKIFGAISDE YDIKEEKPLI TQINDNTYSI LGETTFDEIE EAIGISIKHK EYTNTIGGYL IDLLDKIPTK NETVKTNDGE YFIKEIQNNK IETITFIKSK K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataacaaaa tcataaaaaa aatattaaat ctctttaaag ttaagactag ctcccaaatg actaaagaga gcataaaaaa tatgctctct ttagcaggaa gtttaggaat tttaaaaaat gactctagaa tatttatgca aaaaatgtta gatatagatc aagtaagagc ttctgaaatc atgacccaca gaacaggggt tttttcactc tcaagctcat caaagctaaa agatgtaatc aaattgatca aagaagaagg atactctaga attcctatat acaaaggaca aagcagagaa cagataattg ggattttaat agccaaagac ctcatagaag tcaataaaaa agatatgaat aaaaacgttt ctcaatttat taaaccagct gtatttgtac aacaaaacaa aaggataaaa gacatactag atattatgag gaaaaaacaa aaaataatgg caatcgtaat cgatgaatat ggtggttttt cagggatact tacaatagaa gacatagtag aaaaaatttt tggagcaata tctgacgaat acgacattaa agaggaaaag cctctgatta ctcaaatcaa cgacaatact tactcaatac ttggagaaac tacttttgac gagattgaag aggcaattgg gatatccatt aaacataaag aatatacaaa tacaattggt ggatacttaa tagatctgct tgacaaaata ccaacaaaaa acgaaaccgt aaaaacaaat gatggagaat attttattaa ggaaattcag aataataaaa ttgaaacaat aacttttatc aaatctaaaa agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat