BobuA.01647.a

methionine aminopeptidase (map) (BB_0105)

CENTER ID: BobuA.01647.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01647.a.A1.GE26800 Full length( BobuA.01647.a ) 1 251
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01647.a.A1.PW26875 Full length( BobuA.01647.a ) 1 251
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126721
PATRIC ID: fig|224326.49.peg.503
RefSeq: NP_212239.1
UniProt: O51132
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKLRLKSKD EIKKIKASAS LLALTLLEVE RNIVPGISTK ELDLIAYDFI IKNRAKPAFK GYRGFKGTIC ASVNEEVIHG IPGKRKLADG DIVSIDCGVI LDGFYSDMAK TFKVGNVDSS IDKLLEVTNA SLYKGIAEMK VGNRILNISK AIEDYIKPFG FGIVREYTGH GVGFELHEEP SVPNYYAPFF KNIRIQEGMV LAIEPMVNLR GHKVSIKSDG WTVFASDLSY SAHFEHTVAV VDGLPLILSE V
NT Sequence
TTGACTAAAC TAAGATTGAA ATCTAAAGAT GAGATTAAAA AAATAAAAGC TTCAGCAAGT TTGTTAGCGC TTACTTTGTT AGAAGTTGAG AGAAATATTG TTCCTGGTAT TAGCACCAAG GAGCTTGATT TGATAGCCTA TGATTTTATT ATTAAAAATA GAGCTAAGCC TGCTTTTAAG GGATATCGTG GGTTTAAGGG CACTATTTGC GCATCTGTTA ATGAAGAGGT TATTCACGGC ATTCCCGGAA AAAGAAAATT AGCGGATGGG GATATTGTTA GTATTGACTG TGGGGTTATT CTTGATGGGT TTTATAGCGA TATGGCAAAA ACTTTTAAGG TTGGAAATGT AGATTCTAGT ATCGATAAAC TTTTAGAAGT TACAAATGCC TCTCTTTACA AGGGTATCGC TGAGATGAAA GTTGGAAATA GAATTTTAAA TATATCAAAG GCAATAGAAG ATTATATAAA GCCGTTTGGT TTTGGAATAG TTAGAGAATA TACAGGCCAT GGTGTTGGAT TTGAACTTCA CGAAGAACCA AGTGTTCCAA ATTATTATGC TCCTTTTTTT AAAAATATTA GAATTCAAGA GGGTATGGTT TTGGCTATTG AGCCTATGGT AAACTTAAGG GGGCACAAGG TTTCAATAAA AAGTGATGGT TGGACTGTTT TTGCATCTGA TCTTAGTTAT TCTGCACATT TTGAACATAC TGTTGCTGTT GTTGATGGAT TGCCTTTAAT TTTAAGTGAA GTT
Details for BobuA.01647.a.A1.GE26800
HARVESTED ON: 6/18/2009
SEQUENCED ON: 3/27/2012
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLTKLRLKS KDEIKKIKAS ASLLALTLLE VERNIVPGIS TKELDLIAYD FIIKNRAKPA FKGYRGFKGT ICASVNEEVI HGIPGKRKLA DGDIVSIDCG VILDGFYSDM AKTFKVGNVD SSIDKLLEVT NASLYKGIAE MKVGNRILNI SKAIEDYIKP FGFGIVREYT GHGVGFELHE EPSVPNYYAP FFKNIRIQEG MVLAIEPMVN LRGHKVSIKS DGWTVFASDL SYSARFEHTV AVVDGLPLIL S
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttga ctaaactaag attgaaatct aaagatgaga ttaaaaaaat aaaagcttca gcaagtttgt tagcgcttac tttgttagaa gttgagagaa atattgttcc tggtattagc accaaggagc ttgatttgat agcctatgat tttattatta aaaatagagc taagcctgct tttaagggat atcgtgggtt taagggcact atttgcgcat ctgttaatga agaggttatt cacggcattc ccggaaaaag aaaattagcg gatggggata ttgttagtat tgactgtggg gttattcttg atgggtttta tagcgatatg gcaaaaactt ttaaggttgg aaatgtagat tctagtatcg ataaactttt agaagttaca aatgcctctc tttacaaggg tatcgctgag atgaaagttg gaaatagaat tttaaatata tcaaaggcaa tagaagatta tataaagccg tttggttttg gaatagttag agaatataca ggccatggtg ttggatttga acttcacgaa gaaccaagtg ttccaaatta ttatgctcct ttttttaaaa atattagaat tcaagagggt atggttttgg ctattgagcc tatggtaaac ttaagggggc acaaggtttc aataaaaagt gatggttgga ctgtttttgc atctgatctt agttattctg cacgttttga acatactgtt gctgttgttg atggattgcc tttaatttta agtg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLTKLRLKS KDEIKKIKAS ASLLALTLLE VERNIVPGIS TKELDLIAYD FIIKNRAKPA FKGYRGFKGT ICASVNEEVI HGIPGKRKLA DGDIVSIDCG VILDGFYSDM AKTFKVGNVD SSIDKLLEVT NASLYKGIAE MKVGNRILNI SKAIEDYIKP FGFGIVREYT GHGVGFELHE EPSVPNYYAP FFKNIRIQEG MVLAIEPMVN LRGHKVSIKS DGWTVFASDL SYSAHFEHTV AVVDGLPLIL SEV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgactaaa ctaagattga aatctaaaga tgagattaaa aaaataaaag cttcagcaag tttgttagcg cttactttgt tagaagttga gagaaatatt gttcctggta ttagcaccaa ggagcttgat ttgatagcct atgattttat tattaaaaat agagctaagc ctgcttttaa gggatatcgt gggtttaagg gcactatttg cgcatctgtt aatgaagagg ttattcacgg cattcccgga aaaagaaaat tagcggatgg ggatattgtt agtattgact gtggggttat tcttgatggg ttttatagcg atatggcaaa aacttttaag gttggaaatg tagattctag tatcgataaa cttttagaag ttacaaatgc ctctctttac aagggtatcg ctgagatgaa agttggaaat agaattttaa atatatcaaa ggcaatagaa gattatataa agccgtttgg ttttggaata gttagagaat atacaggcca tggtgttgga tttgaacttc acgaagaacc aagtgttcca aattattatg ctcctttttt taaaaatatt agaattcaag agggtatggt tttggctatt gagcctatgg taaacttaag ggggcacaag gtttcaataa aaagtgatgg ttggactgtt tttgcatctg atcttagtta ttctgcacat tttgaacata ctgttgctgt tgttgatgga ttgcctttaa ttttaagtga agtttaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BobuA.01647.a.A1.PW26875
PURIFICATION DATe: 8/17/2010
CONCENTRATION: 19.27mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLTKLRLKS KDEIKKIKAS ASLLALTLLE VERNIVPGIS TKELDLIAYD FIIKNRAKPA FKGYRGFKGT ICASVNEEVI HGIPGKRKLA DGDIVSIDCG VILDGFYSDM AKTFKVGNVD SSIDKLLEVT NASLYKGIAE MKVGNRILNI SKAIEDYIKP FGFGIVREYT GHGVGFELHE EPSVPNYYAP FFKNIRIQEG MVLAIEPMVN LRGHKVSIKS DGWTVFASDL SYSARFEHTV AVVDGLPLIL S
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttga ctaaactaag attgaaatct aaagatgaga ttaaaaaaat aaaagcttca gcaagtttgt tagcgcttac tttgttagaa gttgagagaa atattgttcc tggtattagc accaaggagc ttgatttgat agcctatgat tttattatta aaaatagagc taagcctgct tttaagggat atcgtgggtt taagggcact atttgcgcat ctgttaatga agaggttatt cacggcattc ccggaaaaag aaaattagcg gatggggata ttgttagtat tgactgtggg gttattcttg atgggtttta tagcgatatg gcaaaaactt ttaaggttgg aaatgtagat tctagtatcg ataaactttt agaagttaca aatgcctctc tttacaaggg tatcgctgag atgaaagttg gaaatagaat tttaaatata tcaaaggcaa tagaagatta tataaagccg tttggttttg gaatagttag agaatataca ggccatggtg ttggatttga acttcacgaa gaaccaagtg ttccaaatta ttatgctcct ttttttaaaa atattagaat tcaagagggt atggttttgg ctattgagcc tatggtaaac ttaagggggc acaaggtttc aataaaaagt gatggttgga ctgtttttgc atctgatctt agttattctg cacgttttga acatactgtt gctgttgttg atggattgcc tttaatttta agtg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLTKLRLKS KDEIKKIKAS ASLLALTLLE VERNIVPGIS TKELDLIAYD FIIKNRAKPA FKGYRGFKGT ICASVNEEVI HGIPGKRKLA DGDIVSIDCG VILDGFYSDM AKTFKVGNVD SSIDKLLEVT NASLYKGIAE MKVGNRILNI SKAIEDYIKP FGFGIVREYT GHGVGFELHE EPSVPNYYAP FFKNIRIQEG MVLAIEPMVN LRGHKVSIKS DGWTVFASDL SYSAHFEHTV AVVDGLPLIL SEV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGACTAAA CTAAGATTGA AATCTAAAGA TGAGATTAAA AAAATAAAAG CTTCAGCAAG TTTGTTAGCG CTTACTTTGT TAGAAGTTGA GAGAAATATT GTTCCTGGTA TTAGCACCAA GGAGCTTGAT TTGATAGCCT ATGATTTTAT TATTAAAAAT AGAGCTAAGC CTGCTTTTAA GGGATATCGT GGGTTTAAGG GCACTATTTG CGCATCTGTT AATGAAGAGG TTATTCACGG CATTCCCGGA AAAAGAAAAT TAGCGGATGG GGATATTGTT AGTATTGACT GTGGGGTTAT TCTTGATGGG TTTTATAGCG ATATGGCAAA AACTTTTAAG GTTGGAAATG TAGATTCTAG TATCGATAAA CTTTTAGAAG TTACAAATGC CTCTCTTTAC AAGGGTATCG CTGAGATGAA AGTTGGAAAT AGAATTTTAA ATATATCAAA GGCAATAGAA GATTATATAA AGCCGTTTGG TTTTGGAATA GTTAGAGAAT ATACAGGCCA TGGTGTTGGA TTTGAACTTC ACGAAGAACC AAGTGTTCCA AATTATTATG CTCCTTTTTT TAAAAATATT AGAATTCAAG AGGGTATGGT TTTGGCTATT GAGCCTATGG TAAACTTAAG GGGGCACAAG GTTTCAATAA AAAGTGATGG TTGGACTGTT TTTGCATCTG ATCTTAGTTA TTCTGCACAT TTTGAACATA CTGTTGCTGT TGTTGATGGA TTGCCTTTAA TTTTAAGTGA AGTTTAAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA