BobuA.01643.a

UDP-N-acetylmuramyl-tripeptide synthetase (murE) (BB_0201)

CENTER ID: BobuA.01643.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01643.a.B1.GE39075 full length 1 508
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.597
RefSeq: NP_212335.1
UniProt: O51219

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKKLNEVLL KLDQDLIKCV KGSLDLEISG VTYSSKLVLP RFVFFALPGI HFDGHDFIEI AIQKGSNVVV CSRDVDFYSP NVTYIKVDDF NIRKFMSNFS NIFYDEPSKK LKVIGVTGTD GKSSVCYYIY LLFKKKGVKV GFISTVFFDD GSGSLIKNPY RQSTPESTEI HSFLSTMVKN EAQYAILEST SHGLDLETAR LIDVNYFAVV FTNIGHEHLE FHGTIQNYLN VKLGLFRSVS DDAGFGVINL DDLYSSDFKN AVKKSFTYSL KSSKADFFVS FIDEKTDSTR FEFYHKGVKY LANVSLLGSF NVENVMAALI LVSQILNIDI QDIVDKLNCI KSLDGRMDSI NLGQNFSVII DYAHTPGAFS KLFPIFKRFA TNRLISVFGS AGERDVEKRF LQGQIADIYS DLIILCDEDP RGENSMCIIK DIAKGIVNKV ENKDLFFIAD RKQAIEKAIS LAKAGDLVVA LGKGHESSII YKNREVFWNE QEVVKNAILS LEKSEKEK
NT Sequence
atgaataaaa aacttaatga agttttatta aagttagatc aagatttaat aaaatgtgta aaaggttctc ttgatttaga aatatcagga gttacttata gttctaaatt ggttttgccc aggtttgtgt tttttgctct tccaggaatt cattttgatg ggcatgattt tattgaaatt gcaattcaaa agggtagtaa tgttgttgtg tgttcacgag atgtggattt ttacagtcct aatgttactt atattaaggt agatgacttt aacataagaa aatttatgtc taatttttca aatatttttt atgatgagcc ttcaaaaaaa ttaaaagtta ttggagtcac tggcactgac gggaaaagtt ctgtttgtta ttatatatat cttcttttta aaaaaaaggg tgttaaagta ggttttatat cgacagtatt ttttgatgat gggagtggaa gcttgattaa aaatccttac agacaatcaa ctcccgagtc tacggaaata cattcatttt taagcaccat ggttaaaaat gaagctcaat atgcaattct tgaatctact tctcatgggc ttgaccttga aacagcaagg cttattgatg ttaattattt tgcagttgtt tttaccaata ttggacatga gcatcttgaa tttcatggca caattcaaaa ttatttgaat gtcaagctgg gtctttttcg gtctgttagt gatgatgctg gttttggggt tattaatctt gatgaccttt attcttctga ttttaagaat gctgttaaga aatcttttac ttatagctta aaaagcagta aagcggattt ttttgttagt tttattgatg agaaaaccga ttctactaga tttgaatttt atcacaaggg ggttaaatat cttgctaatg ttagcctact ggggagtttt aatgttgaga atgtaatggc tgctcttatt ttagtttctc aaattttaaa tatcgatatt caagatattg ttgataaact taactgcatt aaaagtcttg atgggcgtat ggatagtatt aatttggggc aaaatttttc tgtaataatt gattatgctc atactcctgg tgctttttcc aagctttttc ctatttttaa aagatttgct accaatagat tgatttctgt ttttggctct gcaggagaaa gagatgttga aaaaagattt ttgcaagggc aaatcgcaga tatttattct gatttaataa tactttgcga tgaagatcca agaggcgaga atagtatgtg tataattaaa gacattgcaa aaggaattgt aaataaagtt gaaaataagg atttattttt tattgctgat agaaagcagg ctattgaaaa agcaataagt cttgcaaaag caggagattt ggttgttgct ttgggcaaag gtcatgaaag ttcaataatt tataaaaata gagaagtttt ttggaatgaa caagaggtag ttaaaaatgc tattttaagt ttagaaaaat cagaaaagga gaagtga
Details for BobuA.01643.a.B1.GE39075
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Validated AA Sequence
MNKKLNEVLL KLDQDLIKCV KGSLDLEISG VTYSSKLVLP RFVFFALPGI HFDGHDFIEI AIQKGSNVDV CSRDVDFYSP NVTYIKVDDF NIRKFMSNFS NIFYDEPSKK LKVIGVTGTD GKSSVCYYIY LLFKKKGVKV GFISTVFFDD GSGSLIKNPY RQSTPESTEI HSFLSTMVKN EAQYAILEST SHGLDLETAR LIDVNYFAVV FTNIGHEHLE FHGTIQNYLN VKLGLFRSVS DDAGFGVINL DDLYSSDFKN AVKKSFTYSL KSSKADFFVS FIDEKTDSTR FEFYHKGVKY LANVSLLGSF NVENVMAALI LVSQILNIDI QDIVDKLNCI KSLDGRMDSI NLGQNFSVII DYAHTPGAFS KLFPIFKRFA TNRLISVFGS AGERDVEKRF LQGQIADIYS DLIILCDEDP RGENSMCIIK DIAKGIVNKV ENKDLFFIAD RKQAIEKAIS LAKAGDLVVA LGKGHESSII YKNREVFWNE QEVVKNAIL
Validated NT Sequence
ttaaaatagc atttttaact acctcttgtt cattccaaaa aacttctcta tttttataaa ttattgaact ttcatgacct ttgcccaaag caacaaccaa atctcctgct tttgcaagac ttattgcttt ttcaatagcc tgctttctat cagcaataaa aaataaatcc ttattttcaa ctttatttac aattcctttt gcaatgtctt taattataca catactattc tcgcctcttg gatcttcatc gcaaagtatt attaaatcag aataaatatc tgcgatttgc ccttgcaaaa atcttttttc aacatctctt tctcctgcag agccaaaaac agaaatcaat ctattggtag caaatctttt aaaaatagga aaaagcttgg aaaaagcacc aggagtatga gcataatcaa ttattacaga aaaattttgc cccaaattaa tactatccat acgcccatca agacttttaa tgcagttaag tttatcaaca atatcttgaa tatcgatatt taaaatttga gaaactaaaa taagagcagc cattacattc tcaacattaa aactccccag taggctaaca ttagcaagat atttaacccc cttgtgataa aattcaaatc tagtagaatc ggttttctca tcaataaaac taacaaaaaa atccgcttta ctgcttttta agctataagt aaaagatttc ttaacagcat tcttaaaatc agaagaataa aggtcatcaa gattaataac cccaaaacca gcatcatcac taacagaccg aaaaagaccc agcttgacat tcaaataatt ttgaattgtg ccatgaaatt caagatgctc atgtccaata ttggtaaaaa caactgcaaa ataattaaca tcaataagcc ttgctgtttc aaggtcaagc ccatgagaag tagattcaag aattgcatat tgagcttcat ttttaaccat ggtgcttaaa aatgaatgta tttccgtaga ctcgggagtt gattgtctgt aaggattttt aatcaagctt ccactcccat catcaaaaaa tactgtcgat ataaaaccta ctttaacacc ctttttttta aaaagaagat atatataata acaaacagaa cttttcccgt cagtgccagt gactccaata acttttaatt tttttgaagg ctcatcataa aaaatatttg aaaaattaga cataaatttt cttatgttaa agtcatctac cttaatataa gtaacattag gactgtaaaa atccacatct cgtgaacaca catcaacatt actacccttt tgaattgcaa tttcaataaa atcatgccca tcaaaatgaa ttcctggaag agcaaaaaac acaaacctgg gcaaaaccaa tttagaacta taagtaactc ctgatatttc taaatcaaga gaacctttta cacattttat taaatcttga tctaacttta ataaaacttc attaagtttt ttattcatat ggtggtggtg gtggtga
Expected Protein Sequence
MAHHHHHHMN KKLNEVLLKL DQDLIKCVKG SLDLEISGVT YSSKLVLPRF VFFALPGIHF DGHDFIEIAI QKGSNVVVCS RDVDFYSPNV TYIKVDDFNI RKFMSNFSNI FYDEPSKKLK VIGVTGTDGK SSVCYYIYLL FKKKGVKVGF ISTVFFDDGS GSLIKNPYRQ STPESTEIHS FLSTMVKNEA QYAILESTSH GLDLETARLI DVNYFAVVFT NIGHEHLEFH GTIQNYLNVK LGLFRSVSDD AGFGVINLDD LYSSDFKNAV KKSFTYSLKS SKADFFVSFI DEKTDSTRFE FYHKGVKYLA NVSLLGSFNV ENVMAALILV SQILNIDIQD IVDKLNCIKS LDGRMDSINL GQNFSVIIDY AHTPGAFSKL FPIFKRFATN RLISVFGSAG ERDVEKRFLQ GQIADIYSDL IILCDEDPRG ENSMCIIKDI AKGIVNKVEN KDLFFIADRK QAIEKAISLA KAGDLVVALG KGHESSIIYK NREVFWNEQE VVKNAILSLE KSEKEK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata aaaaacttaa tgaagtttta ttaaagttag atcaagattt aataaaatgt gtaaaaggtt ctcttgattt agaaatatca ggagttactt atagttctaa attggttttg cccaggtttg tgttttttgc tcttccagga attcattttg atgggcatga ttttattgaa attgcaattc aaaagggtag taatgttgtt gtgtgttcac gagatgtgga tttttacagt cctaatgtta cttatattaa ggtagatgac tttaacataa gaaaatttat gtctaatttt tcaaatattt tttatgatga gccttcaaaa aaattaaaag ttattggagt cactggcact gacgggaaaa gttctgtttg ttattatata tatcttcttt ttaaaaaaaa gggtgttaaa gtaggtttta tatcgacagt attttttgat gatgggagtg gaagcttgat taaaaatcct tacagacaat caactcccga gtctacggaa atacattcat ttttaagcac catggttaaa aatgaagctc aatatgcaat tcttgaatct acttctcatg ggcttgacct tgaaacagca aggcttattg atgttaatta ttttgcagtt gtttttacca atattggaca tgagcatctt gaatttcatg gcacaattca aaattatttg aatgtcaagc tgggtctttt tcggtctgtt agtgatgatg ctggttttgg ggttattaat cttgatgacc tttattcttc tgattttaag aatgctgtta agaaatcttt tacttatagc ttaaaaagca gtaaagcgga tttttttgtt agttttattg atgagaaaac cgattctact agatttgaat tttatcacaa gggggttaaa tatcttgcta atgttagcct actggggagt tttaatgttg agaatgtaat ggctgctctt attttagttt ctcaaatttt aaatatcgat attcaagata ttgttgataa acttaactgc attaaaagtc ttgatgggcg tatggatagt attaatttgg ggcaaaattt ttctgtaata attgattatg ctcatactcc tggtgctttt tccaagcttt ttcctatttt taaaagattt gctaccaata gattgatttc tgtttttggc tctgcaggag aaagagatgt tgaaaaaaga tttttgcaag ggcaaatcgc agatatttat tctgatttaa taatactttg cgatgaagat ccaagaggcg agaatagtat gtgtataatt aaagacattg caaaaggaat tgtaaataaa gttgaaaata aggatttatt ttttattgct gatagaaagc aggctattga aaaagcaata agtcttgcaa aagcaggaga tttggttgtt gctttgggca aaggtcatga aagttcaata atttataaaa atagagaagt tttttggaat gaacaagagg tagttaaaaa tgctatttta agtttagaaa aatcagaaaa ggagaagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at