BobuA.01616.a

thymidylate kinase (tmk) (BB_0793)

CENTER ID: BobuA.01616.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01616.a.B1.GE39074 full length 1 202
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1185
RefSeq: NP_212927.2
UniProt: O51733

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIKILKNFYC IEGIDGSGKT SITNKLKALC NDESRYYFTK EPSSGIIGEM IRKQLMNFEN PLEESTFAYL YAADRHDHLY KKGGILEILN TKSRKIITDR YLFSSIAYQG KLGYELNKNF PLPEKVFFIE TDPNIAYERI QKNRTQSDLF ELEKYKTFEQ IALKYLKIFK KLEKKINVIY INNSIKDNLD KNAKKIFNLI KF
NT Sequence
gtgattaaaa tattgaaaaa cttttattgc atagaaggaa ttgatggaag cgggaaaaca agcatcacta ataaactaaa agctctttgc aacgatgaat caaggtatta ttttacaaaa gaaccatcaa gtggaataat tggagaaatg ataagaaagc aattaatgaa ttttgaaaat cctttagaag aatcaacatt tgcatatctt tatgctgcag accgacacga tcatttatat aaaaaaggtg gaatactgga aattttaaac acaaaatcta gaaaaataat aactgatcgc tatttattct catcgattgc atatcaagga aaattaggat atgaattaaa taaaaatttc ccattgcctg aaaaagtatt ctttatcgaa acagacccaa acatagctta tgaaagaata cagaaaaata gaacacaaag tgatcttttt gaacttgaaa aatataaaac ttttgaacaa attgctctaa aatatttaaa aatatttaaa aaactagaaa aaaaaattaa tgtgatttac atcaacaatt caataaaaga taatttagat aaaaacgcaa aaaaaatttt caatctaata aaattctaa
Details for BobuA.01616.a.B1.GE39074
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV IKILKNFYCI EGIDGSGKTS ITNKLKALCN YESRYYFTKE PSSGIIGEMI RKQLMNFENP LEESTFAYLY AADRHDHLYK KGGILEILNT KSRKIITDRY LFSSIAYQGK LGYELNKNFP LPEKVFFIET DPNIAYERIQ KNRTQSDLFE LEKYKTFEQI ALKYLKIFKK LEKKINVIYI NNSIKDNLDK NAKKIFNLIK F
Validated NT Sequence
atcttactca cttagaattt tattagattg aaaatttttt ttgcgttttt atctaaatta tcttttattg aattgttgat gtaaatcaca ttaatttttt tttctagttt tttaaatatt tttaaatatt ttagagcaat ttgttcaaaa gttttatatt tttcaagttc aaaaagatca ctttgtgttc tatttttctg tattctttca taagctatgt ttgggtctgt ttcgataaag aatacttttt caggcaatgg gaaattttta tttaattcat atcctaattt tccttgatat gcaatcgatg agaataaata gcgatcagtt attatttttc tagattttgt gtttaaaatt tccagtattc cacctttttt atataaatga tcgtgtcggt ctgcagcata aagatatgca aatgttgatt cttctaaagg attttcaaaa ttcattaatt gctttcttat catttctcca attattccac ttgatggttc ttttgtaaaa taataccttg attcatagtt gcaaagagct tttagtttat tagtgatgct tgttttcccg cttccatcaa ttccttctat gcaataaaag tttttcaata ttttaatcac catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMI KILKNFYCIE GIDGSGKTSI TNKLKALCND ESRYYFTKEP SSGIIGEMIR KQLMNFENPL EESTFAYLYA ADRHDHLYKK GGILEILNTK SRKIITDRYL FSSIAYQGKL GYELNKNFPL PEKVFFIETD PNIAYERIQK NRTQSDLFEL EKYKTFEQIA LKYLKIFKKL EKKINVIYIN NSIKDNLDKN AKKIFNLIKF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgatta aaatattgaa aaacttttat tgcatagaag gaattgatgg aagcgggaaa acaagcatca ctaataaact aaaagctctt tgcaacgatg aatcaaggta ttattttaca aaagaaccat caagtggaat aattggagaa atgataagaa agcaattaat gaattttgaa aatcctttag aagaatcaac atttgcatat ctttatgctg cagaccgaca cgatcattta tataaaaaag gtggaatact ggaaatttta aacacaaaat ctagaaaaat aataactgat cgctatttat tctcatcgat tgcatatcaa ggaaaattag gatatgaatt aaataaaaat ttcccattgc ctgaaaaagt attctttatc gaaacagacc caaacatagc ttatgaaaga atacagaaaa atagaacaca aagtgatctt tttgaacttg aaaaatataa aacttttgaa caaattgctc taaaatattt aaaaatattt aaaaaactag aaaaaaaaat taatgtgatt tacatcaaca attcaataaa agataattta gataaaaacg caaaaaaaat tttcaatcta ataaaattct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat