BobuA.01456.a

acetate kinase (ackA) (BB_0622)

CENTER ID: BobuA.01456.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01456.a.A1.GE26797 Full length( BobuA.01456.a ) 1 413
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01456.a.A1.PS00307 Full length( BobuA.01456.a ) 1 413
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_130287
PATRIC ID: fig|224326.49.peg.1012
RefSeq: NP_212756.1
UniProt: O51567
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNTKGKKMK ILIINTGSSS LKFAIYQYEN SKKIISGIVE KIKSQKSIIK IVNTDGSTTE RFEKGIENHQ KAIEKMFKIL TNSDLKILKT LSEIKIIGHR VVHGGSSLKN SVILNNSILN KLKQISELAP LHNPNAITAI EAVLKILPHA KQVLCFDTSW HQTIKEHAFL YAIPYSWYKN HNIRKYGFHG LSYSYITKRS SEILNKKIDS LNLIILHLGN GASINAVKNG KSYDTSMGIT PLEGLAMGTR SGDIDPSIIN LMSTILNKTT KQIEEILNKE SGILGISEKS NDMRDIWNKI EEGEYQSKLA VEIMTYRIKK YIGSYIAALD FNVDAIVFTG GIGVTDYGIR ALALKGFEKI GIELDLEKNE MAQSKYLESE ISTINSKLKI LAIPTNEEST ILEDIYNLIP KNL
NT Sequence
TTGTCAAATA CAAAAGGAAA AAAAATGAAA ATATTAATCA TAAACACAGG AAGTTCTTCA TTAAAATTTG CTATTTATCA ATATGAAAAT TCAAAAAAAA TAATATCTGG AATTGTTGAA AAAATAAAAT CACAAAAATC AATCATAAAA ATTGTAAATA CTGACGGATC AACAACAGAA AGATTTGAAA AAGGAATTGA AAATCACCAA AAAGCAATAG AAAAAATGTT TAAAATACTC ACAAACAGCG ATTTAAAAAT CCTTAAAACT CTTAGCGAAA TTAAAATAAT AGGACACAGG GTTGTACATG GAGGATCAAG CCTTAAAAAT TCAGTAATTC TTAATAATAG TATTTTAAAT AAATTAAAAC AAATTTCTGA ACTTGCTCCA CTTCATAACC CAAATGCAAT CACCGCAATA GAAGCGGTGC TTAAAATTTT ACCACACGCA AAGCAAGTTT TATGCTTCGA TACATCCTGG CATCAAACTA TAAAAGAACA TGCCTTTCTT TATGCAATTC CATATTCTTG GTATAAAAAC CACAATATTA GAAAATATGG CTTTCATGGC CTTTCTTATT CGTACATAAC AAAAAGATCC TCAGAAATTT TAAATAAAAA AATAGATAGT CTAAATTTAA TAATATTGCA TCTTGGTAAC GGAGCAAGTA TTAATGCTGT TAAAAATGGA AAATCTTATG ACACAAGCAT GGGAATTACT CCGCTTGAAG GCCTTGCAAT GGGAACAAGA AGCGGTGATA TAGACCCATC AATTATTAAT TTGATGAGCA CTATATTAAA CAAAACCACC AAACAAATTG AAGAAATATT AAATAAAGAA AGTGGCATAC TGGGAATTTC TGAAAAATCA AATGACATGC GGGATATTTG GAACAAAATT GAAGAAGGAG AATATCAATC AAAACTTGCA GTAGAAATAA TGACATATAG AATAAAAAAA TATATTGGAT CTTACATTGC CGCTCTTGAT TTTAATGTCG ATGCAATAGT TTTTACAGGC GGAATTGGTG TTACTGATTA TGGAATAAGA GCTCTTGCAC TAAAGGGGTT TGAAAAAATT GGAATAGAAC TGGACCTTGA AAAAAATGAA ATGGCCCAAA GTAAGTATTT AGAATCTGAA ATATCAACCA TTAATAGCAA ACTAAAAATA CTGGCAATAC CAACAAACGA AGAATCAACC ATTCTTGAAG ACATTTATAA TTTAATTCCA AAAAATTTA
Details for BobuA.01456.a.A1.GE26797
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/1/2009
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLSNTKGKK MKILIINTGS SSLKFAIYQY ENSKKIISGI VEKIKSQKSI IKIVNTDGST TERFEKGIEN HQKAIEKMFK ILTNSDLKIL KTLSEIKIIG HRVVHGGSSL KNSVILNNSI LNKLKQISEL APLHNPNAIT AIEAVLKILP HAKQVLCFDT SWHQTIKEHA FLYAIPYSWY KNHNIRKYGF HGLSYSYITK RSSEILNKKI DSLNLIILHL GNGASINAVK NGKSYDTSMG ITPLEGLAMG TRSGDIDPSI INLMSTILNK TTKQIEEILN KESGILGISE KSNDMRDIWN KIEEGEYQSK LAVEIMTYRI KKYIGSYIAA LDFNVDAIVF TGGIGVTDYG IRALALKGFE KIGIELDLEK NEMAQSKYLE SEISTINSKL KILAIPTNEE STILEDIYNL IPK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgt caaatacaaa aggaaaaaaa atgaaaatat taatcataaa cacaggaagt tcttcattaa aatttgctat ttatcaatat gaaaattcaa aaaaaataat atctggaatt gttgaaaaaa taaaatcaca aaaatcaatc ataaaaattg taaatactga cggatcaaca acagaaagat ttgaaaaagg aattgaaaat caccaaaaag caatagaaaa aatgtttaaa atactcacaa acagcgattt aaaaatcctt aaaactctta gcgaaattaa aataatagga cacagggttg tacatggagg atcaagcctt aaaaattcag taattcttaa taatagtatt ttaaataaat taaaacaaat ttctgaactt gctccacttc ataacccaaa tgcaatcacc gcaatagaag cggtgcttaa aattttacca cacgcaaagc aagttttatg cttcgataca tcctggcatc aaactataaa agaacatgcc tttctttatg caattccata ttcttggtat aaaaaccaca atattagaaa atatggcttt catggccttt cttattcgta cataacaaaa agatcctcag aaattttaaa taaaaaaata gatagtctaa atttaataat attgcatctt ggtaacggag caagtattaa tgctgttaaa aatggaaaat cttatgacac aagcatggga attactccgc ttgaaggcct tgcaatggga acaagaagcg gtgatataga cccatcaatt attaatttga tgagcactat attaaacaaa accaccaaac aaattgaaga aatattaaat aaagaaagtg gcatactggg aatttctgaa aaatcaaatg acatgcggga tatttggaac aaaattgaag aaggagaata tcaatcaaaa cttgcagtag aaataatgac atatagaata aaaaaatata ttggatctta cattgccgct cttgatttta atgtcgatgc aatagttttt acaggcggaa ttggtgttac tgattatgga ataagagctc ttgcactaaa ggggtttgaa aaaattggaa tagaactgga ccttgaaaaa aatgaaatgg cccaaagtaa gtatttagaa tctgaaatat caaccattaa tagcaaacta aaaatactgg caataccaac aaacgaagaa tcaaccattc ttgaagacat ttataattta attccaaaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSNTKGKK MKILIINTGS SSLKFAIYQY ENSKKIISGI VEKIKSQKSI IKIVNTDGST TERFEKGIEN HQKAIEKMFK ILTNSDLKIL KTLSEIKIIG HRVVHGGSSL KNSVILNNSI LNKLKQISEL APLHNPNAIT AIEAVLKILP HAKQVLCFDT SWHQTIKEHA FLYAIPYSWY KNHNIRKYGF HGLSYSYITK RSSEILNKKI DSLNLIILHL GNGASINAVK NGKSYDTSMG ITPLEGLAMG TRSGDIDPSI INLMSTILNK TTKQIEEILN KESGILGISE KSNDMRDIWN KIEEGEYQSK LAVEIMTYRI KKYIGSYIAA LDFNVDAIVF TGGIGVTDYG IRALALKGFE KIGIELDLEK NEMAQSKYLE SEISTINSKL KILAIPTNEE STILEDIYNL IPKNL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgtcaaat acaaaaggaa aaaaaatgaa aatattaatc ataaacacag gaagttcttc attaaaattt gctatttatc aatatgaaaa ttcaaaaaaa ataatatctg gaattgttga aaaaataaaa tcacaaaaat caatcataaa aattgtaaat actgacggat caacaacaga aagatttgaa aaaggaattg aaaatcacca aaaagcaata gaaaaaatgt ttaaaatact cacaaacagc gatttaaaaa tccttaaaac tcttagcgaa attaaaataa taggacacag ggttgtacat ggaggatcaa gccttaaaaa ttcagtaatt cttaataata gtattttaaa taaattaaaa caaatttctg aacttgctcc acttcataac ccaaatgcaa tcaccgcaat agaagcggtg cttaaaattt taccacacgc aaagcaagtt ttatgcttcg atacatcctg gcatcaaact ataaaagaac atgcctttct ttatgcaatt ccatattctt ggtataaaaa ccacaatatt agaaaatatg gctttcatgg cctttcttat tcgtacataa caaaaagatc ctcagaaatt ttaaataaaa aaatagatag tctaaattta ataatattgc atcttggtaa cggagcaagt attaatgctg ttaaaaatgg aaaatcttat gacacaagca tgggaattac tccgcttgaa ggccttgcaa tgggaacaag aagcggtgat atagacccat caattattaa tttgatgagc actatattaa acaaaaccac caaacaaatt gaagaaatat taaataaaga aagtggcata ctgggaattt ctgaaaaatc aaatgacatg cgggatattt ggaacaaaat tgaagaagga gaatatcaat caaaacttgc agtagaaata atgacatata gaataaaaaa atatattgga tcttacattg ccgctcttga ttttaatgtc gatgcaatag tttttacagg cggaattggt gttactgatt atggaataag agctcttgca ctaaaggggt ttgaaaaaat tggaatagaa ctggaccttg aaaaaaatga aatggcccaa agtaagtatt tagaatctga aatatcaacc attaatagca aactaaaaat actggcaata ccaacaaacg aagaatcaac cattcttgaa gacatttata atttaattcc aaaaaattta taaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BobuA.01456.a.A1.PS00307
PURIFICATION DATe: 8/6/2009
CONCENTRATION: 25.34mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 20
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLSNTKGKK MKILIINTGS SSLKFAIYQY ENSKKIISGI VEKIKSQKSI IKIVNTDGST TERFEKGIEN HQKAIEKMFK ILTNSDLKIL KTLSEIKIIG HRVVHGGSSL KNSVILNNSI LNKLKQISEL APLHNPNAIT AIEAVLKILP HAKQVLCFDT SWHQTIKEHA FLYAIPYSWY KNHNIRKYGF HGLSYSYITK RSSEILNKKI DSLNLIILHL GNGASINAVK NGKSYDTSMG ITPLEGLAMG TRSGDIDPSI INLMSTILNK TTKQIEEILN KESGILGISE KSNDMRDIWN KIEEGEYQSK LAVEIMTYRI KKYIGSYIAA LDFNVDAIVF TGGIGVTDYG IRALALKGFE KIGIELDLEK NEMAQSKYLE SEISTINSKL KILAIPTNEE STILEDIYNL IPK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgt caaatacaaa aggaaaaaaa atgaaaatat taatcataaa cacaggaagt tcttcattaa aatttgctat ttatcaatat gaaaattcaa aaaaaataat atctggaatt gttgaaaaaa taaaatcaca aaaatcaatc ataaaaattg taaatactga cggatcaaca acagaaagat ttgaaaaagg aattgaaaat caccaaaaag caatagaaaa aatgtttaaa atactcacaa acagcgattt aaaaatcctt aaaactctta gcgaaattaa aataatagga cacagggttg tacatggagg atcaagcctt aaaaattcag taattcttaa taatagtatt ttaaataaat taaaacaaat ttctgaactt gctccacttc ataacccaaa tgcaatcacc gcaatagaag cggtgcttaa aattttacca cacgcaaagc aagttttatg cttcgataca tcctggcatc aaactataaa agaacatgcc tttctttatg caattccata ttcttggtat aaaaaccaca atattagaaa atatggcttt catggccttt cttattcgta cataacaaaa agatcctcag aaattttaaa taaaaaaata gatagtctaa atttaataat attgcatctt ggtaacggag caagtattaa tgctgttaaa aatggaaaat cttatgacac aagcatggga attactccgc ttgaaggcct tgcaatggga acaagaagcg gtgatataga cccatcaatt attaatttga tgagcactat attaaacaaa accaccaaac aaattgaaga aatattaaat aaagaaagtg gcatactggg aatttctgaa aaatcaaatg acatgcggga tatttggaac aaaattgaag aaggagaata tcaatcaaaa cttgcagtag aaataatgac atatagaata aaaaaatata ttggatctta cattgccgct cttgatttta atgtcgatgc aatagttttt acaggcggaa ttggtgttac tgattatgga ataagagctc ttgcactaaa ggggtttgaa aaaattggaa tagaactgga ccttgaaaaa aatgaaatgg cccaaagtaa gtatttagaa tctgaaatat caaccattaa tagcaaacta aaaatactgg caataccaac aaacgaagaa tcaaccattc ttgaagacat ttataattta attccaaaaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSNTKGKK MKILIINTGS SSLKFAIYQY ENSKKIISGI VEKIKSQKSI IKIVNTDGST TERFEKGIEN HQKAIEKMFK ILTNSDLKIL KTLSEIKIIG HRVVHGGSSL KNSVILNNSI LNKLKQISEL APLHNPNAIT AIEAVLKILP HAKQVLCFDT SWHQTIKEHA FLYAIPYSWY KNHNIRKYGF HGLSYSYITK RSSEILNKKI DSLNLIILHL GNGASINAVK NGKSYDTSMG ITPLEGLAMG TRSGDIDPSI INLMSTILNK TTKQIEEILN KESGILGISE KSNDMRDIWN KIEEGEYQSK LAVEIMTYRI KKYIGSYIAA LDFNVDAIVF TGGIGVTDYG IRALALKGFE KIGIELDLEK NEMAQSKYLE SEISTINSKL KILAIPTNEE STILEDIYNL IPKNL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGTCAAAT ACAAAAGGAA AAAAAATGAA AATATTAATC ATAAACACAG GAAGTTCTTC ATTAAAATTT GCTATTTATC AATATGAAAA TTCAAAAAAA ATAATATCTG GAATTGTTGA AAAAATAAAA TCACAAAAAT CAATCATAAA AATTGTAAAT ACTGACGGAT CAACAACAGA AAGATTTGAA AAAGGAATTG AAAATCACCA AAAAGCAATA GAAAAAATGT TTAAAATACT CACAAACAGC GATTTAAAAA TCCTTAAAAC TCTTAGCGAA ATTAAAATAA TAGGACACAG GGTTGTACAT GGAGGATCAA GCCTTAAAAA TTCAGTAATT CTTAATAATA GTATTTTAAA TAAATTAAAA CAAATTTCTG AACTTGCTCC ACTTCATAAC CCAAATGCAA TCACCGCAAT AGAAGCGGTG CTTAAAATTT TACCACACGC AAAGCAAGTT TTATGCTTCG ATACATCCTG GCATCAAACT ATAAAAGAAC ATGCCTTTCT TTATGCAATT CCATATTCTT GGTATAAAAA CCACAATATT AGAAAATATG GCTTTCATGG CCTTTCTTAT TCGTACATAA CAAAAAGATC CTCAGAAATT TTAAATAAAA AAATAGATAG TCTAAATTTA ATAATATTGC ATCTTGGTAA CGGAGCAAGT ATTAATGCTG TTAAAAATGG AAAATCTTAT GACACAAGCA TGGGAATTAC TCCGCTTGAA GGCCTTGCAA TGGGAACAAG AAGCGGTGAT ATAGACCCAT CAATTATTAA TTTGATGAGC ACTATATTAA ACAAAACCAC CAAACAAATT GAAGAAATAT TAAATAAAGA AAGTGGCATA CTGGGAATTT CTGAAAAATC AAATGACATG CGGGATATTT GGAACAAAAT TGAAGAAGGA GAATATCAAT CAAAACTTGC AGTAGAAATA ATGACATATA GAATAAAAAA ATATATTGGA TCTTACATTG CCGCTCTTGA TTTTAATGTC GATGCAATAG TTTTTACAGG CGGAATTGGT GTTACTGATT ATGGAATAAG AGCTCTTGCA CTAAAGGGGT TTGAAAAAAT TGGAATAGAA CTGGACCTTG AAAAAAATGA AATGGCCCAA AGTAAGTATT TAGAATCTGA AATATCAACC ATTAATAGCA AACTAAAAAT ACTGGCAATA CCAACAAACG AAGAATCAAC CATTCTTGAA GACATTTATA ATTTAATTCC AAAAAATTTA TAAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA