SSGCID: Target Page

BobuA.01440.b

translation elongation factor TU (tuf) (BB_0476)

CENTER ID:	BobuA.01440.b
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01440.b.B1.GE40178	Full length( BobuA.01440.b )		1	401

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01440.b.B1.PS38024	Full length( BobuA.01440.b )		1	401

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126954
PATRIC ID:	fig\|224326.49.peg.867
RefSeq:	AAC66866.1
UniProt:	P50062

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKFRRLVMAK EVFQRTKPHM NVGTIGHVDH GKTTLTAAIS IYCSKLNKDA KALKYEDIDN APEEKARGIT INARHIEYET ANRHYAHVDC PGHADYIKNM ITGAAQMDAA ILLVAADSGA EPQTKEHLLL AQRMGIKKII VFLNKLDLAD PELVELVEVE VLELVEKYGF SADTPIIKGS AFGAMSNPED PESTKCVKEL LESMDNYFDL PERDIDKPFL LAVEDVFSIS GRGTVATGRI ERGIIKVGQE VEIVGIKETR KTTVTGVEMF QKILEQGQAG DNVGLLLRGV DKKDIERGQV LSAPGTITPH KKFKASIYCL TKEEGGRHKP FFPGYRPQFF FRTTDVTGVV ALEGKEMVMP GDNVDIIVEL ISSIAMDKNV EFAVREGGRT VASGRILEIL E

NT Sequence

ATGAAATTTA GGAGGTTAGT CATGGCAAAA GAAGTTTTTC AAAGAACAAA GCCGCACATG AATGTTGGAA CAATAGGTCA TGTTGATCAT GGTAAAACAA CACTAACAGC GGCTATTAGT ATTTATTGTT CAAAATTAAA TAAAGATGCA AAAGCATTAA AGTATGAAGA TATTGATAAT GCACCTGAAG AGAAAGCAAG AGGAATAACA ATTAATGCTA GGCATATTGA GTACGAAACA GCTAATAGAC ATTATGCTCA TGTAGATTGT CCAGGCCATG CCGATTATAT AAAAAATATG ATTACAGGAG CAGCTCAAAT GGATGCAGCG ATACTTTTAG TTGCTGCTGA TAGTGGTGCT GAGCCTCAAA CAAAAGAGCA TTTGCTTCTT GCTCAAAGAA TGGGAATAAA GAAAATAATA GTTTTTTTAA ATAAATTGGA CTTAGCAGAT CCTGAACTTG TTGAGCTTGT TGAAGTTGAA GTTTTAGAAC TTGTTGAAAA ATATGGCTTT TCAGCTGATA CTCCAATAAT CAAAGGTTCA GCTTTTGGGG CTATGTCAAA TCCAGAAGAT CCTGAATCTA CAAAATGCGT TAAAGAACTT CTTGAATCTA TGGATAATTA TTTTGATCTT CCAGAAAGAG ATATTGACAA GCCATTTTTG CTTGCTGTTG AAGATGTATT TTCTATTTCA GGAAGAGGCA CTGTTGCTAC TGGGCGTATT GAAAGAGGTA TTATTAAAGT TGGTCAAGAA GTTGAAATAG TTGGAATTAA AGAAACCAGA AAAACTACTG TTACTGGTGT TGAAATGTTC CAGAAAATTC TTGAGCAAGG TCAAGCAGGG GATAATGTTG GTCTTCTTTT GAGAGGCGTT GATAAAAAAG ACATTGAGAG GGGGCAAGTT TTGTCAGCTC CAGGTACAAT TACTCCACAC AAGAAATTTA AAGCTTCAAT TTATTGTTTG ACTAAAGAAG AAGGCGGTAG GCACAAGCCA TTTTTCCCAG GGTATAGACC ACAGTTCTTT TTTAGAACAA CCGATGTTAC TGGAGTTGTT GCTTTAGAGG GCAAAGAAAT GGTTATGCCT GGTGATAATG TTGATATTAT TGTTGAGCTG ATCTCTTCAA TAGCTATGGA TAAGAATGTA GAATTTGCTG TTCGAGAAGG TGGAAGAACC GTTGCTTCAG GAAGAATTCT TGAGATATTG GAA

Details for BobuA.01440.b.B1.GE40178

HARVESTED ON:	5/9/2016
SEQUENCED ON:	5/23/2016
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHHM KFRRLVMAKE VFQRTKPHMN VGTIGHVDHG KTTLTAAISI YCSKLNKDAK ALKYEDIDNA PEEKARGITI NARHIEYETA NRHYAHVDCP GHADYIKNMI TGAAQMDAAI LLVAADSGAE PQTKEHLLLA QRMGIKKIIV FLNKLDLADP ELVELVEVEV LELVEKYGFS ADTPIIKGSA FGAMSNPEDP ESTKCVKELL ESMDNYFDLP ERDIDKPFLL AVEDVFSISG RGTVATGRIE RGIIKVGQEV EIVGIKETRK TTVTGVEMFQ KILEQGQAGD NVGLLLRGVD KKDIERGQVL SAPGTITPHK KFKASIYCLT KEEGGRHKPF FPGYRPQFFF RTTDVTGVVA LEGKEMVMPG DNVDIIVELI SSIAMDKNVE FAVREGGRTV

Validated NT Sequence

agcaacggtt cttccacctt ctcgaacagc aaattctaca ttcttatcca tagctattga agagatcagc tcaacaataa tatcaacatt atcaccaggc ataaccattt ctttgccctc taaagcaaca actccagtaa catcggttgt tctaaaaaag aactgtggtc tataccctgg gaaaaatggc ttgtgcctac cgccttcttc tttagtcaaa caataaattg aagctttaaa tttcttgtgt ggagtaattg tacctggagc tgacaaaact tgccccctct caatgtcttt tttatcaacg cctctcaaaa gaagaccaac attatcccct gcttgacctt gctcaagaat tttctggaac atttcaacac cagtaacagt agtttttctg gtttctttaa ttccaactat ttcaacttct tgaccaactt taataatacc tctttcaata cgcccagtag caacagtgcc tcttcctgaa atagaaaata catcttcaac agcaagcaaa aatggcttgt caatatctct ttctggaaga tcaaaataat tatccataga ttcaagaagt tctttaacgc attttgtaga ttcaggatct tctggatttg acatagcccc aaaagctgaa cctttgatta ttggagtatc agctgaaaag ccatattttt caacaagttc taaaacttca acttcaacaa gctcaacaag ttcaggatct gctaagtcca atttatttaa aaaaactatt attttcttta ttcccattct ttgagcaaga agcaaatgct cttttgtttg aggctcagca ccactatcag cagcaactaa aagtatcgct gcatccattt gagctgctcc tgtaatcata ttttttatat aatcggcatg gcctggacaa tctacatgag cataatgtct attagctgtt tcgtactcaa tatgcctagc attaattgtt attcctcttg ctttctcttc aggtgcatta tcaatatctt catactttaa tgcttttgca tctttattta attttgaaca ataaatacta atagccgctg ttagtgttgt tttaccatga tcaacatgac ctattgttcc aacattcatg tgcggctttg ttctttgaaa aacttctttt gccatgacta acctcctaaa tttcatatgg tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMK FRRLVMAKEV FQRTKPHMNV GTIGHVDHGK TTLTAAISIY CSKLNKDAKA LKYEDIDNAP EEKARGITIN ARHIEYETAN RHYAHVDCPG HADYIKNMIT GAAQMDAAIL LVAADSGAEP QTKEHLLLAQ RMGIKKIIVF LNKLDLADPE LVELVEVEVL ELVEKYGFSA DTPIIKGSAF GAMSNPEDPE STKCVKELLE SMDNYFDLPE RDIDKPFLLA VEDVFSISGR GTVATGRIER GIIKVGQEVE IVGIKETRKT TVTGVEMFQK ILEQGQAGDN VGLLLRGVDK KDIERGQVLS APGTITPHKK FKASIYCLTK EEGGRHKPFF PGYRPQFFFR TTDVTGVVAL EGKEMVMPGD NVDIIVELIS SIAMDKNVEF AVREGGRTVA SGRILEILE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat ttaggaggtt agtcatggca aaagaagttt ttcaaagaac aaagccgcac atgaatgttg gaacaatagg tcatgttgat catggtaaaa caacactaac agcggctatt agtatttatt gttcaaaatt aaataaagat gcaaaagcat taaagtatga agatattgat aatgcacctg aagagaaagc aagaggaata acaattaatg ctaggcatat tgagtacgaa acagctaata gacattatgc tcatgtagat tgtccaggcc atgccgatta tataaaaaat atgattacag gagcagctca aatggatgca gcgatacttt tagttgctgc tgatagtggt gctgagcctc aaacaaaaga gcatttgctt cttgctcaaa gaatgggaat aaagaaaata atagtttttt taaataaatt ggacttagca gatcctgaac ttgttgagct tgttgaagtt gaagttttag aacttgttga aaaatatggc ttttcagctg atactccaat aatcaaaggt tcagcttttg gggctatgtc aaatccagaa gatcctgaat ctacaaaatg cgttaaagaa cttcttgaat ctatggataa ttattttgat cttccagaaa gagatattga caagccattt ttgcttgctg ttgaagatgt attttctatt tcaggaagag gcactgttgc tactgggcgt attgaaagag gtattattaa agttggtcaa gaagttgaaa tagttggaat taaagaaacc agaaaaacta ctgttactgg tgttgaaatg ttccagaaaa ttcttgagca aggtcaagca ggggataatg ttggtcttct tttgagaggc gttgataaaa aagacattga gagggggcaa gttttgtcag ctccaggtac aattactcca cacaagaaat ttaaagcttc aatttattgt ttgactaaag aagaaggcgg taggcacaag ccatttttcc cagggtatag accacagttc ttttttagaa caaccgatgt tactggagtt gttgctttag agggcaaaga aatggttatg cctggtgata atgttgatat tattgttgag ctgatctctt caatagctat ggataagaat gtagaatttg ctgttcgaga aggtggaaga accgttgctt caggaagaat tcttgagata ttggaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for BobuA.01440.b.B1.PS38024

PURIFICATION DATe:	8/17/2016
CONCENTRATION:	35mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAT TTAGGAGGTT AGTCATGGCA AAAGAAGTTT TTCAAAGAAC AAAGCCGCAC ATGAATGTTG GAACAATAGG TCATGTTGAT CATGGTAAAA CAACACTAAC AGCGGCTATT AGTATTTATT GTTCAAAATT AAATAAAGAT GCAAAAGCAT TAAAGTATGA AGATATTGAT AATGCACCTG AAGAGAAAGC AAGAGGAATA ACAATTAATG CTAGGCATAT TGAGTACGAA ACAGCTAATA GACATTATGC TCATGTAGAT TGTCCAGGCC ATGCCGATTA TATAAAAAAT ATGATTACAG GAGCAGCTCA AATGGATGCA GCGATACTTT TAGTTGCTGC TGATAGTGGT GCTGAGCCTC AAACAAAAGA GCATTTGCTT CTTGCTCAAA GAATGGGAAT AAAGAAAATA ATAGTTTTTT TAAATAAATT GGACTTAGCA GATCCTGAAC TTGTTGAGCT TGTTGAAGTT GAAGTTTTAG AACTTGTTGA AAAATATGGC TTTTCAGCTG ATACTCCAAT AATCAAAGGT TCAGCTTTTG GGGCTATGTC AAATCCAGAA GATCCTGAAT CTACAAAATG CGTTAAAGAA CTTCTTGAAT CTATGGATAA TTATTTTGAT CTTCCAGAAA GAGATATTGA CAAGCCATTT TTGCTTGCTG TTGAAGATGT ATTTTCTATT TCAGGAAGAG GCACTGTTGC TACTGGGCGT ATTGAAAGAG GTATTATTAA AGTTGGTCAA GAAGTTGAAA TAGTTGGAAT TAAAGAAACC AGAAAAACTA CTGTTACTGG TGTTGAAATG TTCCAGAAAA TTCTTGAGCA AGGTCAAGCA GGGGATAATG TTGGTCTTCT TTTGAGAGGC GTTGATAAAA AAGACATTGA GAGGGGGCAA GTTTTGTCAG CTCCAGGTAC AATTACTCCA CACAAGAAAT TTAAAGCTTC AATTTATTGT TTGACTAAAG AAGAAGGCGG TAGGCACAAG CCATTTTTCC CAGGGTATAG ACCACAGTTC TTTTTTAGAA CAACCGATGT TACTGGAGTT GTTGCTTTAG AGGGCAAAGA AATGGTTATG CCTGGTGATA ATGTTGATAT TATTGTTGAG CTGATCTCTT CAATAGCTAT GGATAAGAAT GTAGAATTTG CTGTTCGAGA AGGTGGAAGA ACCGTTGCTT CAGGAAGAAT TCTTGAGATA TTGGAATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T