SSGCID: Target Page

BobuA.01440.a

elongation factor Tu

CENTER ID:	BobuA.01440.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01440.a.A1.GE26791	Full length( BobuA.01440.a )		1	394

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01440.a.A1.PS00310	Full length( BobuA.01440.a )		1	394

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.867
RefSeq:	NP_212610.2
UniProt:	P50062

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKEVFQRTK PHMNVGTIGH VDHGKTTLTA AISIYCSKLN KDAKALKYED IDNAPEEKAR GITINARHIE YETANRHYAH VDCPGHADYI KNMITGAAQM DAAILLVAAD SGAEPQTKEH LLLAQRMGIK KIIVFLNKLD LADPELVELV EVEVLELVEK YGFSADTPII KGSAFGAMSN PEDPESTKCV KELLESMDNY FDLPERDIDK PFLLAVEDVF SISGRGTVAT GRIERGIIKV GQEVEIVGIK ETRKTTVTGV EMFQKILEQG QAGDNVGLLL RGVDKKDIER GQVLSAPGTI TPHKKFKASI YCLTKEEGGR HKPFFPGYRP QFFFRTTDVT GVVALEGKEM VMPGDNVDII VELISSIAMD KNVEFAVREG GRTVASGRIL EILE

NT Sequence

ATGGCAAAAG AAGTTTTTCA AAGAACAAAG CCGCACATGA ATGTTGGAAC AATAGGTCAT GTTGATCATG GTAAAACAAC ACTAACAGCG GCTATTAGTA TTTATTGTTC AAAATTAAAT AAAGATGCAA AAGCATTAAA GTATGAAGAT ATTGATAATG CACCTGAAGA GAAAGCAAGA GGAATAACAA TTAATGCTAG GCATATTGAG TACGAAACAG CTAATAGACA TTATGCTCAT GTAGATTGTC CAGGCCATGC CGATTATATA AAAAATATGA TTACAGGAGC AGCTCAAATG GATGCAGCGA TACTTTTAGT TGCTGCTGAT AGTGGTGCTG AGCCTCAAAC AAAAGAGCAT TTGCTTCTTG CTCAAAGAAT GGGAATAAAG AAAATAATAG TTTTTTTAAA TAAATTGGAC TTAGCAGATC CTGAACTTGT TGAGCTTGTT GAAGTTGAAG TTTTAGAACT TGTTGAAAAA TATGGCTTTT CAGCTGATAC TCCAATAATC AAAGGTTCAG CTTTTGGGGC TATGTCAAAT CCAGAAGATC CTGAATCTAC AAAATGCGTT AAAGAACTTC TTGAATCTAT GGATAATTAT TTTGATCTTC CAGAAAGAGA TATTGACAAG CCATTTTTGC TTGCTGTTGA AGATGTATTT TCTATTTCAG GAAGAGGCAC TGTTGCTACT GGGCGTATTG AAAGAGGTAT TATTAAAGTT GGTCAAGAAG TTGAAATAGT TGGAATTAAA GAAACCAGAA AAACTACTGT TACTGGTGTT GAAATGTTCC AGAAAATTCT TGAGCAAGGT CAAGCAGGGG ATAATGTTGG TCTTCTTTTG AGAGGCGTTG ATAAAAAAGA CATTGAGAGG GGGCAAGTTT TGTCAGCTCC AGGTACAATT ACTCCACACA AGAAATTTAA AGCTTCAATT TATTGTTTGA CTAAAGAAGA AGGCGGTAGG CACAAGCCAT TTTTCCCAGG GTATAGACCA CAGTTCTTTT TTAGAACAAC CGATGTTACT GGAGTTGTTG CTTTAGAGGG CAAAGAAATG GTTATGCCTG GTGATAATGT TGATATTATT GTTGAGCTGA TCTCTTCAAT AGCTATGGAT AAGAATGTAG AATTTGCTGT TCGAGAAGGT GGAAGAACCG TTGCTTCAGG AAGAATTCTT GAGATATTGG AA

Details for BobuA.01440.a.A1.GE26791

HARVESTED ON:	6/8/2009
SEQUENCED ON:	3/27/2012
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	73

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAKEVFQRT KPHMNVGTIG HVDHGKTTLT AAISIYCSKL NKDAKALKYE DIDNAPEEKA RGITINARHI EYETANRHYA HVDCPGHADY IKNMITGAAQ MDAAILLVAA DSGAEPQTKE HLLLAQRMGI KKIIVFLNKL DLADPELVEL VEVEVLELVE KYGFSADTPI IKGSAFGAMS NPEDPESTKC VKELLESMDN YFDLPERDID KPFLLAVEDV FSISGRGTVA TGRIERXIIK VGQEVEIVGI KETRKTTVTG VEMXQKILEX GQAXDNXGLL LXGVXKX

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcaa aagaagtttt tcaaagaaca aagccgcaca tgaatgttgg aacaataggt catgttgatc atggtaaaac aacactaaca gcggctatta gtatttattg ttcaaaatta aataaagatg caaaagcatt aaagtatgaa gatattgata atgcacctga agagaaagca agaggaataa caattaatgc taggcatatt gagtacgaaa cagctaatag acattatgct catgtagatt gtccaggcca tgccgattat ataaaaaata tgattacagg agcagctcaa atggatgcag cgatactttt agttgctgct gatagtggtg ctgagcctca aacaaaagag catttgcttc ttgctcaaag aatgggaata aagaaaataa tagttttttt aaataaattg gacttagcag atcctgaact tgttgagctt gttgaagttg aagttttaga acttgttgaa aaatatggct tttcagctga tactccaata atcaaaggtt cagcttttgg ggctatgtca aatccagaag atcctgaatc tacaaaatgc gttaaagaac ttcttgaatc tatggataat tattttgatc ttccagaaag agatattgac aagccatttt tgcttgctgt tgaagatgta ttttctattt caggaagagg cactgttgct actgggcgta ttgaaagang tattattaaa gttggtcaag aagttgaaat agttggaatt aaagaaacca gaaaaactac tgttactggt gttgaaatgn tccagaaaat tcttgagcan ggncaagcnn gggataatgn tggtcttctt ttganaggng ttganaaaaa na

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAKEVFQRT KPHMNVGTIG HVDHGKTTLT AAISIYCSKL NKDAKALKYE DIDNAPEEKA RGITINARHI EYETANRHYA HVDCPGHADY IKNMITGAAQ MDAAILLVAA DSGAEPQTKE HLLLAQRMGI KKIIVFLNKL DLADPELVEL VEVEVLELVE KYGFSADTPI IKGSAFGAMS NPEDPESTKC VKELLESMDN YFDLPERDID KPFLLAVEDV FSISGRGTVA TGRIERGIIK VGQEVEIVGI KETRKTTVTG VEMFQKILEQ GQAGDNVGLL LRGVDKKDIE RGQVLSAPGT ITPHKKFKAS IYCLTKEEGG RHKPFFPGYR PQFFFRTTDV TGVVALEGKE MVMPGDNVDI IVELISSIAM DKNVEFAVRE GGRTVASGRI LEILE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaaaagaa gtttttcaaa gaacaaagcc gcacatgaat gttggaacaa taggtcatgt tgatcatggt aaaacaacac taacagcggc tattagtatt tattgttcaa aattaaataa agatgcaaaa gcattaaagt atgaagatat tgataatgca cctgaagaga aagcaagagg aataacaatt aatgctaggc atattgagta cgaaacagct aatagacatt atgctcatgt agattgtcca ggccatgccg attatataaa aaatatgatt acaggagcag ctcaaatgga tgcagcgata cttttagttg ctgctgatag tggtgctgag cctcaaacaa aagagcattt gcttcttgct caaagaatgg gaataaagaa aataatagtt tttttaaata aattggactt agcagatcct gaacttgttg agcttgttga agttgaagtt ttagaacttg ttgaaaaata tggcttttca gctgatactc caataatcaa aggttcagct tttggggcta tgtcaaatcc agaagatcct gaatctacaa aatgcgttaa agaacttctt gaatctatgg ataattattt tgatcttcca gaaagagata ttgacaagcc atttttgctt gctgttgaag atgtattttc tatttcagga agaggcactg ttgctactgg gcgtattgaa agaggtatta ttaaagttgg tcaagaagtt gaaatagttg gaattaaaga aaccagaaaa actactgtta ctggtgttga aatgttccag aaaattcttg agcaaggtca agcaggggat aatgttggtc ttcttttgag aggcgttgat aaaaaagaca ttgagagggg gcaagttttg tcagctccag gtacaattac tccacacaag aaatttaaag cttcaattta ttgtttgact aaagaagaag gcggtaggca caagccattt ttcccagggt atagaccaca gttctttttt agaacaaccg atgttactgg agttgttgct ttagagggca aagaaatggt tatgcctggt gataatgttg atattattgt tgagctgatc tcttcaatag ctatggataa gaatgtagaa tttgctgttc gagaaggtgg aagaaccgtt gcttcaggaa gaattcttga gatattggaa tagaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BobuA.01440.a.A1.PS00310

PURIFICATION DATe:	8/7/2009
CONCENTRATION:	30.7mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	15
VIAL VOLUME:	100µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	73

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAKEVFQRT KPHMNVGTIG HVDHGKTTLT AAISIYCSKL NKDAKALKYE DIDNAPEEKA RGITINARHI EYETANRHYA HVDCPGHADY IKNMITGAAQ MDAAILLVAA DSGAEPQTKE HLLLAQRMGI KKIIVFLNKL DLADPELVEL VEVEVLELVE KYGFSADTPI IKGSAFGAMS NPEDPESTKC VKELLESMDN YFDLPERDID KPFLLAVEDV FSISGRGTVA TGRIERGIIK VGQEVEIVGI KETRKTTVTG VEMFQKILEQ GQAGDNVGLL LRGVDKKDIE RGQVLSAPGT ITPHKKFKAS IYCLTKEEGG RHKPFFPGYR PQFFFRTTDV TGVVALEGKE MVMPGDNVDI IVELISSIAM DKNVEFAVRE GGRTVASGRI LEILE

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAAAAGAA GTTTTTCAAA GAACAAAGCC GCACATGAAT GTTGGAACAA TAGGTCATGT TGATCATGGT AAAACAACAC TAACAGCGGC TATTAGTATT TATTGTTCAA AATTAAATAA AGATGCAAAA GCATTAAAGT ATGAAGATAT TGATAATGCA CCTGAAGAGA AAGCAAGAGG AATAACAATT AATGCTAGGC ATATTGAGTA CGAAACAGCT AATAGACATT ATGCTCATGT AGATTGTCCA GGCCATGCCG ATTATATAAA AAATATGATT ACAGGAGCAG CTCAAATGGA TGCAGCGATA CTTTTAGTTG CTGCTGATAG TGGTGCTGAG CCTCAAACAA AAGAGCATTT GCTTCTTGCT CAAAGAATGG GAATAAAGAA AATAATAGTT TTTTTAAATA AATTGGACTT AGCAGATCCT GAACTTGTTG AGCTTGTTGA AGTTGAAGTT TTAGAACTTG TTGAAAAATA TGGCTTTTCA GCTGATACTC CAATAATCAA AGGTTCAGCT TTTGGGGCTA TGTCAAATCC AGAAGATCCT GAATCTACAA AATGCGTTAA AGAACTTCTT GAATCTATGG ATAATTATTT TGATCTTCCA GAAAGAGATA TTGACAAGCC ATTTTTGCTT GCTGTTGAAG ATGTATTTTC TATTTCAGGA AGAGGCACTG TTGCTACTGG GCGTATTGAA AGAGGTATTA TTAAAGTTGG TCAAGAAGTT GAAATAGTTG GAATTAAAGA AACCAGAAAA ACTACTGTTA CTGGTGTTGA AATGTTCCAG AAAATTCTTG AGCAAGGTCA AGCAGGGGAT AATGTTGGTC TTCTTTTGAG AGGCGTTGAT AAAAAAGACA TTGAGAGGGG GCAAGTTTTG TCAGCTCCAG GTACAATTAC TCCACACAAG AAATTTAAAG CTTCAATTTA TTGTTTGACT AAAGAAGAAG GCGGTAGGCA CAAGCCATTT TTCCCAGGGT ATAGACCACA GTTCTTTTTT AGAACAACCG ATGTTACTGG AGTTGTTGCT TTAGAGGGCA AAGAAATGGT TATGCCTGGT GATAATGTTG ATATTATTGT TGAGCTGATC TCTTCAATAG CTATGGATAA GAATGTAGAA TTTGCTGTTC GAGAAGGTGG AAGAACCGTT GCTTCAGGAA GAATTCTTGA GATATTGGAA TAGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA