SSGCID: Target Page

BobuA.01402.a

fructose-bisphosphate aldolase (BB_0445)

CENTER ID:	BobuA.01402.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01402.a.A1.GE26787	Full length( BobuA.01402.a )		1	359

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01402.a.A1.PS00334	Full length( BobuA.01402.a )		1	359

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_131639
PATRIC ID:	fig\|224326.49.peg.836
RefSeq:	NP_212579.1
UniProt:	O51401

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGVLDKIKPG VVYGKELHFL YEICKKEGFA IPSINCIGTN SINAVLEAAK EINSPIMIQF SNSGSAFISG KGLKMEKPQG VSIVGAISGA MHVHLMAEHY GVPVVLHTDH CAKNLLPWVE GLLEYGEKYY SQHKKPLFSS HMLDLSEEPI KENIEISKKF LERMAKIEMF LEIELGITGG EEDGVDNSDR ALHELFSTPE DIYYGYSELL KVSPNFQIAA AFGNVHGVYK PGNVKLTPKV LKDGQDYVIS KTGVNMAKPV SYVFHGGSGS TIDEINEALS YGVVKMNIDT DTQWAAWEGV LNYYKKNESR LQGQLGDGKD IDIPNKKFYD PRVWLREAEV SMKDRVKIAC KNLNNINRN

NT Sequence

ATGGGTGTTT TAGATAAGAT TAAACCAGGT GTAGTTTATG GGAAGGAACT ACATTTTTTA TATGAAATAT GTAAAAAGGA AGGATTTGCT ATTCCTTCTA TTAATTGTAT AGGAACAAAT TCTATTAATG CAGTTTTGGA GGCAGCAAAA GAAATTAATT CTCCTATTAT GATACAATTT TCTAATAGTG GATCTGCTTT TATTTCTGGA AAAGGATTAA AGATGGAAAA ACCACAAGGA GTTTCAATAG TTGGAGCTAT TTCTGGTGCT ATGCATGTTC ATTTAATGGC AGAGCATTAT GGTGTTCCTG TTGTTCTTCA TACTGATCAC TGTGCTAAAA ATTTGCTTCC TTGGGTTGAA GGCCTTTTAG AATATGGAGA GAAATACTAT AGTCAGCACA AAAAACCATT ATTTTCTTCA CATATGTTAG ATTTATCAGA AGAACCTATT AAAGAAAATA TTGAAATTTC TAAAAAATTC TTAGAAAGAA TGGCAAAAAT TGAAATGTTT TTGGAAATAG AGCTTGGAAT TACGGGTGGG GAAGAGGATG GAGTTGACAA TTCAGATAGA GCTTTGCATG AACTATTTTC TACTCCTGAG GATATTTATT ATGGATATTC AGAACTTTTA AAAGTTAGCC CAAATTTTCA GATTGCAGCA GCTTTTGGAA ATGTTCATGG GGTATATAAA CCGGGGAATG TTAAGCTTAC TCCAAAAGTT TTAAAAGATG GTCAAGATTA TGTCATATCA AAAACAGGAG TAAATATGGC TAAGCCAGTT TCTTATGTTT TTCATGGAGG GTCTGGATCT ACAATTGATG AGATTAATGA GGCGCTTTCT TATGGCGTTG TAAAGATGAA TATTGACACA GATACACAGT GGGCTGCCTG GGAGGGTGTT TTAAATTATT ACAAAAAAAA TGAAAGTCGT TTGCAAGGTC AATTAGGAGA TGGCAAGGAT ATTGATATTC CAAATAAGAA ATTTTATGAT CCAAGGGTTT GGTTAAGAGA AGCTGAAGTT TCTATGAAAG ACCGTGTGAA GATTGCATGC AAAAATCTTA ATAATATTAA TAGAAAT

Details for BobuA.01402.a.A1.GE26787

HARVESTED ON:	6/1/2009
SEQUENCED ON:	7/15/2009
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGVLDKIKP GVVYGKELHF LYEICKKEGF AIPSINCIGT NSINAVLEAA KEINSPIMIQ FSNSGSAFIS GKGLKMEKPQ GVSIVGAISG AMHVHLMAEH YGVPVVLHTD HCAKNLLPWV EGLLEYGEKY YSQHKKPLFS SHMLDLSEEP IKENIEISKK FLERMAKIEM FLEIELGITG GEEDGVDNSD RALHELFSTP EDIYYGYSEL LKVSPNFQIA AAFGNVHGVY KPGNVKLTPK VLKDGQDYVI SKTGVNMAKP VSYVFHGGSG STIDEINEAL SYGVVKMNID TDTQWAAWEG VLNYYKKNES RLQGQLGDGK DIDIPNKKFY DPRVWLREAE VSMKDRVKIA CKNLNNINRN

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggtg ttttagataa gattaaacca ggtgtagttt atgggaagga actacatttt ttatatgaaa tatgtaaaaa ggaaggattt gctattcctt ctattaattg tataggaaca aattctatta atgcagtttt ggaggcagca aaagaaatta attctcctat tatgatacaa ttttctaata gtggatctgc ttttatttct ggaaaaggat taaagatgga aaaaccacaa ggagtttcaa tagttggagc tatttctggt gctatgcatg ttcatttaat ggcagagcat tatggtgttc ctgttgttct tcatactgat cactgtgcta aaaatttgct tccttgggtt gaaggccttt tagaatatgg agagaaatac tatagtcagc acaaaaaacc attattttct tcacatatgt tagatttatc agaagaacct attaaagaaa atattgaaat ttctaaaaaa ttcttagaaa gaatggcaaa aattgaaatg tttttggaaa tagagcttgg aattacgggt ggggaagagg atggagttga caattcagat agagctttgc atgaactatt ttctactcct gaggatattt attatggata ttcagaactt ttaaaagtta gcccaaattt tcagattgca gcagcttttg gaaatgttca tggggtatat aaaccgggga atgttaagct tactccaaaa gttttaaaag atggtcaaga ttatgtcata tcaaaaacag gagtaaatat ggctaagcca gtttcttatg tttttcatgg agggtctgga tctacaattg atgagattaa tgaggcgctt tcttatggcg ttgtaaagat gaatattgac acagatacac agtgggctgc ctgggagggt gttttaaatt attacaaaaa aaatgaaagt cgtttgcaag gtcaattagg agatggcaag gatattgata ttccaaataa gaaattttat gatccaaggg tttggttaag agaagctgaa gtttctatga aagaccgtgt gaagattgca tgcaaaaatc ttaataatat taatagaaat taataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtgtttta gataagatta aaccaggtgt agtttatggg aaggaactac attttttata tgaaatatgt aaaaaggaag gatttgctat tccttctatt aattgtatag gaacaaattc tattaatgca gttttggagg cagcaaaaga aattaattct cctattatga tacaattttc taatagtgga tctgctttta tttctggaaa aggattaaag atggaaaaac cacaaggagt ttcaatagtt ggagctattt ctggtgctat gcatgttcat ttaatggcag agcattatgg tgttcctgtt gttcttcata ctgatcactg tgctaaaaat ttgcttcctt gggttgaagg ccttttagaa tatggagaga aatactatag tcagcacaaa aaaccattat tttcttcaca tatgttagat ttatcagaag aacctattaa agaaaatatt gaaatttcta aaaaattctt agaaagaatg gcaaaaattg aaatgttttt ggaaatagag cttggaatta cgggtgggga agaggatgga gttgacaatt cagatagagc tttgcatgaa ctattttcta ctcctgagga tatttattat ggatattcag aacttttaaa agttagccca aattttcaga ttgcagcagc ttttggaaat gttcatgggg tatataaacc ggggaatgtt aagcttactc caaaagtttt aaaagatggt caagattatg tcatatcaaa aacaggagta aatatggcta agccagtttc ttatgttttt catggagggt ctggatctac aattgatgag attaatgagg cgctttctta tggcgttgta aagatgaata ttgacacaga tacacagtgg gctgcctggg agggtgtttt aaattattac aaaaaaaatg aaagtcgttt gcaaggtcaa ttaggagatg gcaaggatat tgatattcca aataagaaat tttatgatcc aagggtttgg ttaagagaag ctgaagtttc tatgaaagac cgtgtgaaga ttgcatgcaa aaatcttaat aatattaata gaaattaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for BobuA.01402.a.A1.PS00334

PURIFICATION DATe:	8/20/2009
CONCENTRATION:	40.9mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGTGTTTTA GATAAGATTA AACCAGGTGT AGTTTATGGG AAGGAACTAC ATTTTTTATA TGAAATATGT AAAAAGGAAG GATTTGCTAT TCCTTCTATT AATTGTATAG GAACAAATTC TATTAATGCA GTTTTGGAGG CAGCAAAAGA AATTAATTCT CCTATTATGA TACAATTTTC TAATAGTGGA TCTGCTTTTA TTTCTGGAAA AGGATTAAAG ATGGAAAAAC CACAAGGAGT TTCAATAGTT GGAGCTATTT CTGGTGCTAT GCATGTTCAT TTAATGGCAG AGCATTATGG TGTTCCTGTT GTTCTTCATA CTGATCACTG TGCTAAAAAT TTGCTTCCTT GGGTTGAAGG CCTTTTAGAA TATGGAGAGA AATACTATAG TCAGCACAAA AAACCATTAT TTTCTTCACA TATGTTAGAT TTATCAGAAG AACCTATTAA AGAAAATATT GAAATTTCTA AAAAATTCTT AGAAAGAATG GCAAAAATTG AAATGTTTTT GGAAATAGAG CTTGGAATTA CGGGTGGGGA AGAGGATGGA GTTGACAATT CAGATAGAGC TTTGCATGAA CTATTTTCTA CTCCTGAGGA TATTTATTAT GGATATTCAG AACTTTTAAA AGTTAGCCCA AATTTTCAGA TTGCAGCAGC TTTTGGAAAT GTTCATGGGG TATATAAACC GGGGAATGTT AAGCTTACTC CAAAAGTTTT AAAAGATGGT CAAGATTATG TCATATCAAA AACAGGAGTA AATATGGCTA AGCCAGTTTC TTATGTTTTT CATGGAGGGT CTGGATCTAC AATTGATGAG ATTAATGAGG CGCTTTCTTA TGGCGTTGTA AAGATGAATA TTGACACAGA TACACAGTGG GCTGCCTGGG AGGGTGTTTT AAATTATTAC AAAAAAAATG AAAGTCGTTT GCAAGGTCAA TTAGGAGATG GCAAGGATAT TGATATTCCA AATAAGAAAT TTTATGATCC AAGGGTTTGG TTAAGAGAAG CTGAAGTTTC TATGAAAGAC CGTGTGAAGA TTGCATGCAA AAATCTTAAT AATATTAATA GAAATTAAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA