BobuA.01384.a

chemotaxis protein methyltransferase (cheR-1)

CENTER ID: BobuA.01384.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01384.a.B1.GE26950 Full length( BobuA.01384.a ) 1 283
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01384.a.B1.PW26930 Full length( BobuA.01384.a ) 1 283

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.439
RefSeq: NP_212174.1
UniProt: O51069

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTNQNKFNL NINITKDELS RLIKIVYNNF GINLSEKKKL LIESRLSSLL KVKGFKNFTE YINFLEKSTG NIQLIELIDK ISTNHTYFFR ESKHFDFLNN KILPKLTEKI LNSENSEIRI WSAGCSSGEE PYTIAMMLKE YMEHNRVNFK VKILATDISI SVLNEAREGI YPEDRIINLP KYLKIKYLNQ LQDDKFQVKE ILKKMVYFKK LNLMDEKFPF SKKFDLIFCR NVMIYFDEKT RNNLANKFNS YLKKDSYLLI GHSETIRGNK NLEYIMPATY KKN
NT Sequence
ATGAACACGA ATCAAAACAA ATTCAACCTT AATATAAATA TAACTAAGGA CGAACTTTCA AGATTAATAA AAATAGTTTA CAATAATTTT GGAATCAATC TCAGCGAAAA AAAAAAGCTG CTAATTGAAA GCAGATTATC ATCTCTTCTG AAAGTTAAAG GTTTTAAAAA TTTTACTGAA TACATCAACT TTTTAGAAAA AAGCACTGGA AATATTCAAT TAATCGAATT AATAGACAAA ATATCAACAA ATCATACCTA TTTTTTCAGA GAATCTAAGC ATTTTGATTT TTTAAATAAT AAAATATTAC CCAAACTTAC TGAAAAAATA TTAAACTCAG AAAACTCAGA AATTAGAATA TGGTCAGCTG GCTGTTCAAG TGGTGAAGAG CCTTATACAA TTGCAATGAT GCTAAAAGAA TATATGGAAC ATAATAGAGT AAATTTTAAA GTCAAAATCT TAGCAACAGA CATTTCAATT AGCGTTCTAA ATGAGGCTCG TGAAGGGATT TATCCTGAAG ATCGCATAAT AAATTTGCCC AAATATTTAA AAATTAAATA TTTAAATCAA CTTCAAGATG ATAAATTTCA AGTTAAAGAA ATTTTAAAAA AAATGGTTTA CTTTAAAAAA TTAAATTTAA TGGATGAGAA ATTTCCATTT AGCAAAAAAT TTGACCTAAT ATTTTGCAGA AATGTCATGA TCTATTTTGA TGAAAAAACT AGGAATAACC TTGCCAATAA ATTCAACTCC TACCTTAAAA AGGATTCTTA TCTTTTAATT GGACATTCAG AAACAATCAG AGGAAACAAA AATCTTGAAT ACATAATGCC TGCCACCTAT AAAAAGAAT
Details for BobuA.01384.a.B1.GE26950
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/15/2009
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN TNQNKFNLNI NITKDELSRL IKIVYNNFGI NLSEKKKLLI ESRLSSLLKV KGFKNFTEYI NFLEKSTGNI QLIELIDKIS TNHTYFFRES KHFDFLNNKI LPKLTEKILN SENSEIRIWS AGCSSGEEPY TIAMMLKEYM EHNRVNFKVK ILATDISISV LNEAREGIYP EDRIINLPKY LKIKYLNQLQ DDKFQVKEIL KKMVYFKKLN LMDEKFPFSK KFDLIFCRNV MIYFDEKTRN NLANKFNSYL KKDSYLLIGH SETIRGNKNL EYIMPATYKK N
Validated NT Sequence
atcttactca cttaattctt tttataggtg gcaggcatta tgtattcaag atttttgttt cctctgattg tttctgaatg tccaattaaa agataagaat cctttttaag gtaggagttg aatttattgg caaggttatt cctagttttt tcatcaaaat agatcatgac atttctgcaa aatattaggt caaatttttt gctaaatgga aatttctcat ccattaaatt taatttttta aagtaaacca ttttttttaa aatttcttta acttgaaatt tatcatcttg aagttgattt aaatatttaa tttttaaata tttgggcaaa tttattatgc gatcttcagg ataaatccct tcacgagcct catttagaac gctaattgaa atgtctgttg ctaagatttt gactttaaaa tttactctat tatgttccat atattctttt agcatcattg caattgtata aggctcttca ccacttgaac agccagctga ccatattcta atttctgagt tttctgagtt taatattttt tcagtaagtt tgggtaatat tttattattt aaaaaatcaa aatgcttaga ttctctgaaa aaataggtat gatttgttga tattttgtct attaattcga ttaattgaat atttccagtg cttttttcta aaaagttgat gtattcagta aaatttttaa aacctttaac tttcagaaga gatgataatc tgctttcaat tagcagcttt tttttttcgc tgagattgat tccaaaatta ttgtaaacta tttttattaa tcttgaaagt tcgtccttag ttatatttat attaaggttg aatttgtttt gattcgtgtt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMN TNQNKFNLNI NITKDELSRL IKIVYNNFGI NLSEKKKLLI ESRLSSLLKV KGFKNFTEYI NFLEKSTGNI QLIELIDKIS TNHTYFFRES KHFDFLNNKI LPKLTEKILN SENSEIRIWS AGCSSGEEPY TIAMMLKEYM EHNRVNFKVK ILATDISISV LNEAREGIYP EDRIINLPKY LKIKYLNQLQ DDKFQVKEIL KKMVYFKKLN LMDEKFPFSK KFDLIFCRNV MIYFDEKTRN NLANKFNSYL KKDSYLLIGH SETIRGNKNL EYIMPATYKK N
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca cgaatcaaaa caaattcaac cttaatataa atataactaa ggacgaactt tcaagattaa taaaaatagt ttacaataat tttggaatca atctcagcga aaaaaaaaag ctgctaattg aaagcagatt atcatctctt ctgaaagtta aaggttttaa aaattttact gaatacatca actttttaga aaaaagcact ggaaatattc aattaatcga attaatagac aaaatatcaa caaatcatac ctattttttc agagaatcta agcattttga ttttttaaat aataaaatat tacccaaact tactgaaaaa atattaaact cagaaaactc agaaattaga atatggtcag ctggctgttc aagtggtgaa gagccttata caattgcaat gatgctaaaa gaatatatgg aacataatag agtaaatttt aaagtcaaaa tcttagcaac agacatttca attagcgttc taaatgaggc tcgtgaaggg atttatcctg aagatcgcat aataaatttg cccaaatatt taaaaattaa atatttaaat caacttcaag atgataaatt tcaagttaaa gaaattttaa aaaaaatggt ttactttaaa aaattaaatt taatggatga gaaatttcca tttagcaaaa aatttgacct aatattttgc agaaatgtca tgatctattt tgatgaaaaa actaggaata accttgccaa taaattcaac tcctacctta aaaaggattc ttatctttta attggacatt cagaaacaat cagaggaaac aaaaatcttg aatacataat gcctgccacc tataaaaaga attaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BobuA.01384.a.B1.PW26930
PURIFICATION DATe: 8/3/2009
CONCENTRATION: 23.13mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN TNQNKFNLNI NITKDELSRL IKIVYNNFGI NLSEKKKLLI ESRLSSLLKV KGFKNFTEYI NFLEKSTGNI QLIELIDKIS TNHTYFFRES KHFDFLNNKI LPKLTEKILN SENSEIRIWS AGCSSGEEPY TIAMMLKEYM EHNRVNFKVK ILATDISISV LNEAREGIYP EDRIINLPKY LKIKYLNQLQ DDKFQVKEIL KKMVYFKKLN LMDEKFPFSK KFDLIFCRNV MIYFDEKTRN NLANKFNSYL KKDSYLLIGH SETIRGNKNL EYIMPATYKK N
Validated NT Sequence
atcttactca cttaattctt tttataggtg gcaggcatta tgtattcaag atttttgttt cctctgattg tttctgaatg tccaattaaa agataagaat cctttttaag gtaggagttg aatttattgg caaggttatt cctagttttt tcatcaaaat agatcatgac atttctgcaa aatattaggt caaatttttt gctaaatgga aatttctcat ccattaaatt taatttttta aagtaaacca ttttttttaa aatttcttta acttgaaatt tatcatcttg aagttgattt aaatatttaa tttttaaata tttgggcaaa tttattatgc gatcttcagg ataaatccct tcacgagcct catttagaac gctaattgaa atgtctgttg ctaagatttt gactttaaaa tttactctat tatgttccat atattctttt agcatcattg caattgtata aggctcttca ccacttgaac agccagctga ccatattcta atttctgagt tttctgagtt taatattttt tcagtaagtt tgggtaatat tttattattt aaaaaatcaa aatgcttaga ttctctgaaa aaataggtat gatttgttga tattttgtct attaattcga ttaattgaat atttccagtg cttttttcta aaaagttgat gtattcagta aaatttttaa aacctttaac tttcagaaga gatgataatc tgctttcaat tagcagcttt tttttttcgc tgagattgat tccaaaatta ttgtaaacta tttttattaa tcttgaaagt tcgtccttag ttatatttat attaaggttg aatttgtttt gattcgtgtt catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMN TNQNKFNLNI NITKDELSRL IKIVYNNFGI NLSEKKKLLI ESRLSSLLKV KGFKNFTEYI NFLEKSTGNI QLIELIDKIS TNHTYFFRES KHFDFLNNKI LPKLTEKILN SENSEIRIWS AGCSSGEEPY TIAMMLKEYM EHNRVNFKVK ILATDISISV LNEAREGIYP EDRIINLPKY LKIKYLNQLQ DDKFQVKEIL KKMVYFKKLN LMDEKFPFSK KFDLIFCRNV MIYFDEKTRN NLANKFNSYL KKDSYLLIGH SETIRGNKNL EYIMPATYKK N
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACA CGAATCAAAA CAAATTCAAC CTTAATATAA ATATAACTAA GGACGAACTT TCAAGATTAA TAAAAATAGT TTACAATAAT TTTGGAATCA ATCTCAGCGA AAAAAAAAAG CTGCTAATTG AAAGCAGATT ATCATCTCTT CTGAAAGTTA AAGGTTTTAA AAATTTTACT GAATACATCA ACTTTTTAGA AAAAAGCACT GGAAATATTC AATTAATCGA ATTAATAGAC AAAATATCAA CAAATCATAC CTATTTTTTC AGAGAATCTA AGCATTTTGA TTTTTTAAAT AATAAAATAT TACCCAAACT TACTGAAAAA ATATTAAACT CAGAAAACTC AGAAATTAGA ATATGGTCAG CTGGCTGTTC AAGTGGTGAA GAGCCTTATA CAATTGCAAT GATGCTAAAA GAATATATGG AACATAATAG AGTAAATTTT AAAGTCAAAA TCTTAGCAAC AGACATTTCA ATTAGCGTTC TAAATGAGGC TCGTGAAGGG ATTTATCCTG AAGATCGCAT AATAAATTTG CCCAAATATT TAAAAATTAA ATATTTAAAT CAACTTCAAG ATGATAAATT TCAAGTTAAA GAAATTTTAA AAAAAATGGT TTACTTTAAA AAATTAAATT TAATGGATGA GAAATTTCCA TTTAGCAAAA AATTTGACCT AATATTTTGC AGAAATGTCA TGATCTATTT TGATGAAAAA ACTAGGAATA ACCTTGCCAA TAAATTCAAC TCCTACCTTA AAAAGGATTC TTATCTTTTA ATTGGACATT CAGAAACAAT CAGAGGAAAC AAAAATCTTG AATACATAAT GCCTGCCACC TATAAAAAGA ATTAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT