BobuA.01363.a

phosphoglycerate kinase (BB_0056)

CENTER ID: BobuA.01363.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01363.a.A1.GE26615 Full length( BobuA.01363.a ) 1 393
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01363.a.A1.PW26685 Full length( BobuA.01363.a ) 1 393
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126776
PATRIC ID: fig|224326.49.peg.454
RefSeq: NP_212190.1
UniProt: Q59181
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIKTVKDFS SFAGKRALVR CDFNVPLKEG SISDDTRIRA ALSTIEYLKE RGARIVLVSH LGRPDGKKNP KYSLKPVANR LSELLGQDVK MLSDCIGSEV VNSTLQMKDG DVVLLENVRF YAEEEKNDKN FAKKLSENGD VFVNDAFGAA HRAHASTVGV ADYLPSVGGF LMEKEDKFLG GILKNPERPF VSIIGGSKVS SKIAVLESLL SKSNVVVIGG GMAYTFLHSE GYSIGKSLLE DEYIGIASSF LKKAKELGVK VILPLDHIVA DDFNKNSIPE YIDSFNIPEN KIGMDIGANT LKEIENVVKT AKTIIWNGPL GVFEFDSFSK GTAKVAEMVA SCSGLTVVGG GDSVAAVNKF NLSDKITHVS TGGGASLEYL EGRILPGIKV LEN
NT Sequence
ATGTCAATAA AAACAGTAAA AGACTTTAGT AGCTTTGCGG GCAAGCGAGC TTTAGTAAGA TGTGATTTTA ATGTTCCCCT AAAAGAGGGG AGCATTAGCG ATGATACTAG AATTAGGGCA GCATTGTCTA CAATAGAGTA TCTAAAAGAA AGAGGAGCTA GGATTGTGCT TGTAAGTCAT TTGGGTAGAC CAGATGGAAA GAAAAATCCT AAATATTCTC TTAAGCCGGT TGCCAATAGG TTGTCAGAGC TTTTAGGCCA AGATGTCAAG ATGCTTTCTG ATTGTATTGG AAGTGAGGTT GTAAATAGTA CCTTGCAAAT GAAGGATGGG GATGTTGTAT TGCTTGAAAA TGTAAGGTTT TATGCAGAAG AAGAGAAAAA CGATAAAAAT TTTGCAAAAA AACTTTCTGA AAATGGAGAT GTTTTTGTAA ATGATGCTTT TGGCGCAGCT CATAGAGCTC ATGCTTCAAC TGTGGGAGTT GCTGATTATT TGCCGTCTGT TGGTGGATTT TTAATGGAAA AAGAAGATAA ATTTCTTGGT GGAATATTAA AAAATCCCGA AAGGCCATTT GTTTCAATTA TTGGTGGTTC TAAGGTTTCT TCAAAGATTG CGGTACTAGA ATCACTTTTG TCAAAGTCTA ATGTAGTTGT GATTGGCGGT GGAATGGCTT ATACTTTTTT GCATTCCGAA GGATATTCTA TAGGCAAATC TCTTTTAGAG GACGAATATA TTGGTATAGC ATCTTCTTTT TTAAAGAAAG CAAAAGAGCT GGGCGTTAAA GTAATTTTAC CACTTGATCA TATTGTAGCT GATGATTTTA ACAAAAATTC AATTCCTGAG TATATTGACT CCTTTAATAT TCCTGAAAAC AAGATTGGCA TGGATATTGG AGCTAATACT TTAAAAGAAA TTGAAAATGT TGTGAAAACT GCCAAAACTA TAATTTGGAA TGGTCCTCTT GGTGTATTTG AATTTGATTC ATTTTCAAAA GGGACTGCTA AGGTTGCCGA AATGGTAGCA TCTTGCTCAG GTTTAACCGT TGTTGGTGGT GGAGATTCTG TTGCAGCTGT TAATAAATTT AATTTATCTG ATAAGATTAC TCATGTTTCA ACGGGCGGTG GTGCATCGCT TGAATATCTT GAAGGGAGAA TTTTGCCGGG TATTAAGGTT TTGGAGAAT
Details for BobuA.01363.a.A1.GE26615
HARVESTED ON: 4/23/2009
SEQUENCED ON: 5/21/2009
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSIKTVKDF SSFAGKRALV RCDFNVPLKE GSISDDTRIR AALSTIEYLK ERGARIVLVS HLGRPDGKKN PKYSLKPVAN RLSELLGQDV KMLSDCIGSE VVNSTLQMKD GDVVLLENVR FYAEEEKNDK NFAKKLSENG DVFVNDAFGA AHRAHASTVG VADYLPSVGG FLMEKEDKFL GGILKNPERP FVSIIGGSKV SSKIAVLESL LSKSNVVVIG GGMAYTFLHS EGYSIGKSLL EDEYIGIASS FLKKAKELGV KVILPLDHIV ADDFNKNSIP EYIDSFNIPE NKIGMDIGAN TLKEIENVVK TAKTIIWNGP LGVFEFDSFS KGTAKVAEMV ASCSGLTVVG GGDSVAAVNK FNLSDKITHV STGGGASLEY LEGRILPGIK VL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcaa taaaaacagt aaaagacttt agtagctttg cgggcaagcg agctttagta agatgtgatt ttaatgttcc cctaaaagag gggagcatta gcgatgatac tagaattagg gcagcattgt ctacaataga gtatctaaaa gaaagaggag ctaggattgt gcttgtaagt catttgggta gaccagatgg aaagaaaaat cctaaatatt ctcttaagcc ggttgccaat aggttgtcag agcttttagg ccaagatgtc aagatgcttt ctgattgtat tggaagtgag gttgtaaata gtaccttgca aatgaaggat ggggatgttg tattgcttga aaatgtaagg ttttatgcag aagaagagaa aaacgataaa aattttgcaa aaaaactttc tgaaaatgga gatgtttttg taaatgatgc ttttggcgca gctcatagag ctcatgcttc aactgtggga gttgctgatt atttgccgtc tgttggtgga tttttaatgg aaaaagaaga taaatttctt ggtggaatat taaaaaatcc cgaaaggcca tttgtttcaa ttattggtgg ttctaaggtt tcttcaaaga ttgcggtact agaatcactt ttgtcaaagt ctaatgtagt tgtgattggc ggtggaatgg cttatacttt tttgcattcc gaaggatatt ctataggcaa atctctttta gaggacgaat atattggtat agcatcttct tttttaaaga aagcaaaaga gctgggcgtt aaagtaattt taccacttga tcatattgta gctgatgatt ttaacaaaaa ttcaattcct gagtatattg actcctttaa tattcctgaa aacaagattg gcatggatat tggagctaat actttaaaag aaattgaaaa tgttgtgaaa actgccaaaa ctataatttg gaatggtcct cttggtgtat ttgaatttga ttcattttca aaagggactg ctaaggttgc cgaaatggta gcatcttgct caggtttaac cgttgttggt ggtggagatt ctgttgcagc tgttaataaa tttaatttat ctgataagat tactcatgtt tcaacgggcg gtggtgcatc gcttgaatat cttgaaggga gaattttgcc gggtattaag gttttgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIKTVKDF SSFAGKRALV RCDFNVPLKE GSISDDTRIR AALSTIEYLK ERGARIVLVS HLGRPDGKKN PKYSLKPVAN RLSELLGQDV KMLSDCIGSE VVNSTLQMKD GDVVLLENVR FYAEEEKNDK NFAKKLSENG DVFVNDAFGA AHRAHASTVG VADYLPSVGG FLMEKEDKFL GGILKNPERP FVSIIGGSKV SSKIAVLESL LSKSNVVVIG GGMAYTFLHS EGYSIGKSLL EDEYIGIASS FLKKAKELGV KVILPLDHIV ADDFNKNSIP EYIDSFNIPE NKIGMDIGAN TLKEIENVVK TAKTIIWNGP LGVFEFDSFS KGTAKVAEMV ASCSGLTVVG GGDSVAAVNK FNLSDKITHV STGGGASLEY LEGRILPGIK VLEN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcaataaaa acagtaaaag actttagtag ctttgcgggc aagcgagctt tagtaagatg tgattttaat gttcccctaa aagaggggag cattagcgat gatactagaa ttagggcagc attgtctaca atagagtatc taaaagaaag aggagctagg attgtgcttg taagtcattt gggtagacca gatggaaaga aaaatcctaa atattctctt aagccggttg ccaataggtt gtcagagctt ttaggccaag atgtcaagat gctttctgat tgtattggaa gtgaggttgt aaatagtacc ttgcaaatga aggatgggga tgttgtattg cttgaaaatg taaggtttta tgcagaagaa gagaaaaacg ataaaaattt tgcaaaaaaa ctttctgaaa atggagatgt ttttgtaaat gatgcttttg gcgcagctca tagagctcat gcttcaactg tgggagttgc tgattatttg ccgtctgttg gtggattttt aatggaaaaa gaagataaat ttcttggtgg aatattaaaa aatcccgaaa ggccatttgt ttcaattatt ggtggttcta aggtttcttc aaagattgcg gtactagaat cacttttgtc aaagtctaat gtagttgtga ttggcggtgg aatggcttat acttttttgc attccgaagg atattctata ggcaaatctc ttttagagga cgaatatatt ggtatagcat cttctttttt aaagaaagca aaagagctgg gcgttaaagt aattttacca cttgatcata ttgtagctga tgattttaac aaaaattcaa ttcctgagta tattgactcc tttaatattc ctgaaaacaa gattggcatg gatattggag ctaatacttt aaaagaaatt gaaaatgttg tgaaaactgc caaaactata atttggaatg gtcctcttgg tgtatttgaa tttgattcat tttcaaaagg gactgctaag gttgccgaaa tggtagcatc ttgctcaggt ttaaccgttg ttggtggtgg agattctgtt gcagctgtta ataaatttaa tttatctgat aagattactc atgtttcaac gggcggtggt gcatcgcttg aatatcttga agggagaatt ttgccgggta ttaaggtttt ggagaattaa aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BobuA.01363.a.A1.PW26685
PURIFICATION DATe: 7/13/2009
CONCENTRATION: 26.95mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSIKTVKDF SSFAGKRALV RCDFNVPLKE GSISDDTRIR AALSTIEYLK ERGARIVLVS HLGRPDGKKN PKYSLKPVAN RLSELLGQDV KMLSDCIGSE VVNSTLQMKD GDVVLLENVR FYAEEEKNDK NFAKKLSENG DVFVNDAFGA AHRAHASTVG VADYLPSVGG FLMEKEDKFL GGILKNPERP FVSIIGGSKV SSKIAVLESL LSKSNVVVIG GGMAYTFLHS EGYSIGKSLL EDEYIGIASS FLKKAKELGV KVILPLDHIV ADDFNKNSIP EYIDSFNIPE NKIGMDIGAN TLKEIENVVK TAKTIIWNGP LGVFEFDSFS KGTAKVAEMV ASCSGLTVVG GGDSVAAVNK FNLSDKITHV STGGGASLEY LEGRILPGIK VL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcaa taaaaacagt aaaagacttt agtagctttg cgggcaagcg agctttagta agatgtgatt ttaatgttcc cctaaaagag gggagcatta gcgatgatac tagaattagg gcagcattgt ctacaataga gtatctaaaa gaaagaggag ctaggattgt gcttgtaagt catttgggta gaccagatgg aaagaaaaat cctaaatatt ctcttaagcc ggttgccaat aggttgtcag agcttttagg ccaagatgtc aagatgcttt ctgattgtat tggaagtgag gttgtaaata gtaccttgca aatgaaggat ggggatgttg tattgcttga aaatgtaagg ttttatgcag aagaagagaa aaacgataaa aattttgcaa aaaaactttc tgaaaatgga gatgtttttg taaatgatgc ttttggcgca gctcatagag ctcatgcttc aactgtggga gttgctgatt atttgccgtc tgttggtgga tttttaatgg aaaaagaaga taaatttctt ggtggaatat taaaaaatcc cgaaaggcca tttgtttcaa ttattggtgg ttctaaggtt tcttcaaaga ttgcggtact agaatcactt ttgtcaaagt ctaatgtagt tgtgattggc ggtggaatgg cttatacttt tttgcattcc gaaggatatt ctataggcaa atctctttta gaggacgaat atattggtat agcatcttct tttttaaaga aagcaaaaga gctgggcgtt aaagtaattt taccacttga tcatattgta gctgatgatt ttaacaaaaa ttcaattcct gagtatattg actcctttaa tattcctgaa aacaagattg gcatggatat tggagctaat actttaaaag aaattgaaaa tgttgtgaaa actgccaaaa ctataatttg gaatggtcct cttggtgtat ttgaatttga ttcattttca aaagggactg ctaaggttgc cgaaatggta gcatcttgct caggtttaac cgttgttggt ggtggagatt ctgttgcagc tgttaataaa tttaatttat ctgataagat tactcatgtt tcaacgggcg gtggtgcatc gcttgaatat cttgaaggga gaattttgcc gggtattaag gttttgg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIKTVKDF SSFAGKRALV RCDFNVPLKE GSISDDTRIR AALSTIEYLK ERGARIVLVS HLGRPDGKKN PKYSLKPVAN RLSELLGQDV KMLSDCIGSE VVNSTLQMKD GDVVLLENVR FYAEEEKNDK NFAKKLSENG DVFVNDAFGA AHRAHASTVG VADYLPSVGG FLMEKEDKFL GGILKNPERP FVSIIGGSKV SSKIAVLESL LSKSNVVVIG GGMAYTFLHS EGYSIGKSLL EDEYIGIASS FLKKAKELGV KVILPLDHIV ADDFNKNSIP EYIDSFNIPE NKIGMDIGAN TLKEIENVVK TAKTIIWNGP LGVFEFDSFS KGTAKVAEMV ASCSGLTVVG GGDSVAAVNK FNLSDKITHV STGGGASLEY LEGRILPGIK VLEN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCAATAAAA ACAGTAAAAG ACTTTAGTAG CTTTGCGGGC AAGCGAGCTT TAGTAAGATG TGATTTTAAT GTTCCCCTAA AAGAGGGGAG CATTAGCGAT GATACTAGAA TTAGGGCAGC ATTGTCTACA ATAGAGTATC TAAAAGAAAG AGGAGCTAGG ATTGTGCTTG TAAGTCATTT GGGTAGACCA GATGGAAAGA AAAATCCTAA ATATTCTCTT AAGCCGGTTG CCAATAGGTT GTCAGAGCTT TTAGGCCAAG ATGTCAAGAT GCTTTCTGAT TGTATTGGAA GTGAGGTTGT AAATAGTACC TTGCAAATGA AGGATGGGGA TGTTGTATTG CTTGAAAATG TAAGGTTTTA TGCAGAAGAA GAGAAAAACG ATAAAAATTT TGCAAAAAAA CTTTCTGAAA ATGGAGATGT TTTTGTAAAT GATGCTTTTG GCGCAGCTCA TAGAGCTCAT GCTTCAACTG TGGGAGTTGC TGATTATTTG CCGTCTGTTG GTGGATTTTT AATGGAAAAA GAAGATAAAT TTCTTGGTGG AATATTAAAA AATCCCGAAA GGCCATTTGT TTCAATTATT GGTGGTTCTA AGGTTTCTTC AAAGATTGCG GTACTAGAAT CACTTTTGTC AAAGTCTAAT GTAGTTGTGA TTGGCGGTGG AATGGCTTAT ACTTTTTTGC ATTCCGAAGG ATATTCTATA GGCAAATCTC TTTTAGAGGA CGAATATATT GGTATAGCAT CTTCTTTTTT AAAGAAAGCA AAAGAGCTGG GCGTTAAAGT AATTTTACCA CTTGATCATA TTGTAGCTGA TGATTTTAAC AAAAATTCAA TTCCTGAGTA TATTGACTCC TTTAATATTC CTGAAAACAA GATTGGCATG GATATTGGAG CTAATACTTT AAAAGAAATT GAAAATGTTG TGAAAACTGC CAAAACTATA ATTTGGAATG GTCCTCTTGG TGTATTTGAA TTTGATTCAT TTTCAAAAGG GACTGCTAAG GTTGCCGAAA TGGTAGCATC TTGCTCAGGT TTAACCGTTG TTGGTGGTGG AGATTCTGTT GCAGCTGTTA ATAAATTTAA TTTATCTGAT AAGATTACTC ATGTTTCAAC GGGCGGTGGT GCATCGCTTG AATATCTTGA AGGGAGAATT TTGCCGGGTA TTAAGGTTTT GGAGAATTAA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA