BobuA.01348.a
glutamyl-tRNA synthetase (BB_0372)
| CENTER ID: | BobuA.01348.a |
| ORGANISM: | Borrelia burgdorferi B31 |
| ASSOCIATED DISEASE: | Lyme disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.01348.a.A1.GE26613 | Full length( BobuA.01348.a ) | 1 | 490 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.01348.a.A1.PW27582 | Full length( BobuA.01348.a ) | 1 | 490 |
Structures
4GRI
DEPOSITED: 8/24/2012
DETERMINATION: XRay
CLONE: BobuA.01348.a.A1.GE26613
PROTEIN: BobuA.01348.a.A1.PW26683
Publications by SSGCID
Ligand co-crystallization of aminoacyl-tRNA synthetases from infectious disease organisms
Clifton MC, Dranow DM, Edwards TE, Lorimer DD, Manoil C, Moen S, Myler PJ, Sankaran B, Sharma A, Staker BL, Van Voorhis WC
Sci Rep - 2017
volume 7, issue 1, pages 223
PMID: 28303005; PMCID: PMC5428304
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127621 |
| PATRIC ID: | fig|224326.49.peg.767 |
| RefSeq: | NP_212506.1 |
| UniProt: | O51345 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTRVRYAPS PTGLQHIGGI RTALFNYFFA KSCGGKFLLR IEDTDQSRYS PEAENDLYSS LKWLGISFDE GPVVGGDYAP YVQSQRSAIY KQYAKYLIES GHAYYCYCSP ERLERIKKIQ NINKMPPGYD RHCRNLSNEE VENALIKKIK PVVRFKIPLE GDTSFDDILL GRITWANKDI SPDPVILKSD GLPTYHLANV VDDYLMKITH VLRAQEWVSS GPLHVLLYKA FKWKPPIYCH LPMVMGNDGQ KLSKRHGSTA LRQFIEDGYL PEAIINYVTL LGWSYDDKRE FFSKNDLEQF FSIEKINKSP AIFDYHKLDF FNSYYIREKK DEDLFNLLLP FFQKKGYVSK PSTLEENQKL KLLIPLIKSR IKKLSDALNM TKFFYEDIKS WNLDEFLSRK KTAKEVCSIL ELIKPILEGF EKRSSEENDK IFYDFAESNG FKLGEILLPI RIAALGSKVS PPLFDSLKLI GKSKVFERIK LAQEFLRINE
NT Sequence
TTGAGTACAA GAGTTCGTTA TGCGCCTTCT CCAACGGGTT TACAACATAT TGGCGGGATT AGAACAGCTT TGTTTAATTA TTTTTTTGCA AAGTCTTGCG GAGGTAAATT TTTGCTTAGA ATTGAGGATA CAGATCAGAG CAGGTATTCT CCAGAAGCTG AAAATGATCT TTATTCAAGT CTTAAATGGC TTGGCATTTC TTTTGATGAA GGCCCTGTTG TAGGGGGTGA TTATGCGCCT TATGTTCAGT CTCAAAGAAG TGCAATATAT AAGCAATATG CTAAATATTT GATTGAATCT GGGCATGCTT ATTATTGTTA TTGCAGTCCC GAAAGGCTTG AAAGAATTAA GAAAATTCAA AATATTAATA AGATGCCACC TGGATATGAT AGGCATTGCA GGAATTTAAG TAATGAAGAG GTTGAGAATG CACTAATTAA AAAAATCAAG CCTGTTGTTA GATTTAAAAT TCCTTTAGAA GGAGATACCA GCTTTGATGA TATTTTACTT GGAAGGATTA CATGGGCGAA TAAAGACATT AGTCCTGATC CTGTAATTCT TAAGTCAGAT GGATTGCCGA CTTATCATCT TGCCAATGTT GTTGATGATT ATTTAATGAA AATTACCCAT GTATTAAGGG CTCAAGAATG GGTTTCTTCA GGTCCATTGC ACGTACTTCT TTATAAGGCT TTTAAATGGA AACCCCCTAT TTATTGTCAC CTTCCAATGG TTATGGGAAA TGATGGTCAA AAATTAAGCA AAAGACATGG CTCAACAGCT TTAAGACAGT TTATTGAAGA TGGGTATCTT CCAGAGGCTA TTATTAATTA TGTTACTTTG CTTGGCTGGT CTTACGACGA TAAGAGAGAA TTTTTTTCAA AAAATGATCT TGAGCAATTT TTTTCAATTG AGAAGATCAA TAAATCTCCT GCTATTTTTG ATTATCATAA ATTGGATTTT TTCAATAGCT ACTATATTAG AGAAAAAAAA GATGAAGATT TATTTAATCT TTTACTCCCT TTTTTCCAAA AAAAAGGGTA TGTTTCTAAG CCTAGTACTT TGGAAGAAAA TCAAAAATTA AAGTTATTAA TTCCTCTTAT AAAGAGTAGA ATTAAAAAAT TAAGTGATGC TTTAAATATG ACTAAATTTT TTTATGAGGA CATTAAATCT TGGAATTTAG ATGAGTTTTT AAGTAGAAAA AAAACAGCTA AAGAAGTTTG TTCTATTTTA GAATTAATAA AGCCTATTTT AGAAGGGTTT GAAAAAAGAT CATCAGAAGA AAATGATAAA ATTTTTTATG ATTTTGCTGA GAGTAATGGT TTTAAATTGG GAGAAATTCT TCTTCCTATT AGAATTGCAG CGCTTGGTAG CAAAGTCTCT CCGCCGCTTT TTGATTCTTT AAAATTGATA GGCAAGTCTA AAGTTTTTGA AAGAATAAAA TTAGCACAGG AATTTTTAAG AATAAATGAA
Details for BobuA.01348.a.A1.GE26613
| HARVESTED ON: | 4/23/2009 |
| SEQUENCED ON: | 5/21/2009 |
| EXPECTED MW: | 59kDa |
| OBSERVED MW: | 59kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 94 |
| PERCENT COVERAGE: | 38 |
Validated AA Sequence
MAHHHHHHMX TLEAQTQGPG SMLSTRVRYA PSPTGLQHIG GIRTALFNYF FAKSCGGKFL LRIEDTDQSR YSPEAENDLY SSLKWLGISF DEGPVVGGDY APYVQSQRSA IYKQYAKYLI ESGHAYYXYC SPEXLERIKK IQNINKMPPG YDXHCRNLSX EEVEXALIKK IKPVVRFKIP LXGXTXFDDX LLGRI
Validated NT Sequence
ccatggctca tcaccatcac catcatatgn ntaccctgga agctcagacc cagggtcctg gttcgatgtt gagtacaaga gttcgttatg cgccttctcc aacgggttta caacatattg gcgggattag aacagctttg tttaattatt tttttgcaaa gtcttgcgga ggtaaatttt tgcttagaat tgaggataca gatcagagca ggtattctcc agaagctgaa aatgatcttt attcaagtct taaatggctt ggcatttctt ttgatgaagg ccctgttgta gggggtgatt atgcgcctta tgttcagtct caaagaagtg cnatatataa gcaatatgct aaatatttga ttgaatctgg gcatgcttat tattnttatt gcagtcccga anggcttgaa agaattaaga aaattcaaaa tattaataag atgccacctg gatatgatng gcattgcagg aatttaagta angaagaggt tgaganngca ctaattaaaa aaatcaagcc tgttgttaga tttaaaattc ctttanaagg nnnnaccnnn tttgatgatn ttttacttgg aaggatta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSTRVRYA PSPTGLQHIG GIRTALFNYF FAKSCGGKFL LRIEDTDQSR YSPEAENDLY SSLKWLGISF DEGPVVGGDY APYVQSQRSA IYKQYAKYLI ESGHAYYCYC SPERLERIKK IQNINKMPPG YDRHCRNLSN EEVENALIKK IKPVVRFKIP LEGDTSFDDI LLGRITWANK DISPDPVILK SDGLPTYHLA NVVDDYLMKI THVLRAQEWV SSGPLHVLLY KAFKWKPPIY CHLPMVMGND GQKLSKRHGS TALRQFIEDG YLPEAIINYV TLLGWSYDDK REFFSKNDLE QFFSIEKINK SPAIFDYHKL DFFNSYYIRE KKDEDLFNLL LPFFQKKGYV SKPSTLEENQ KLKLLIPLIK SRIKKLSDAL NMTKFFYEDI KSWNLDEFLS RKKTAKEVCS ILELIKPILE GFEKRSSEEN DKIFYDFAES NGFKLGEILL PIRIAALGSK VSPPLFDSLK LIGKSKVFER IKLAQEFLRI NE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgagtaca agagttcgtt atgcgccttc tccaacgggt ttacaacata ttggcgggat tagaacagct ttgtttaatt atttttttgc aaagtcttgc ggaggtaaat ttttgcttag aattgaggat acagatcaga gcaggtattc tccagaagct gaaaatgatc tttattcaag tcttaaatgg cttggcattt cttttgatga aggccctgtt gtagggggtg attatgcgcc ttatgttcag tctcaaagaa gtgcaatata taagcaatat gctaaatatt tgattgaatc tgggcatgct tattattgtt attgcagtcc cgaaaggctt gaaagaatta agaaaattca aaatattaat aagatgccac ctggatatga taggcattgc aggaatttaa gtaatgaaga ggttgagaat gcactaatta aaaaaatcaa gcctgttgtt agatttaaaa ttcctttaga aggagatacc agctttgatg atattttact tggaaggatt acatgggcga ataaagacat tagtcctgat cctgtaattc ttaagtcaga tggattgccg acttatcatc ttgccaatgt tgttgatgat tatttaatga aaattaccca tgtattaagg gctcaagaat gggtttcttc aggtccattg cacgtacttc tttataaggc ttttaaatgg aaacccccta tttattgtca ccttccaatg gttatgggaa atgatggtca aaaattaagc aaaagacatg gctcaacagc tttaagacag tttattgaag atgggtatct tccagaggct attattaatt atgttacttt gcttggctgg tcttacgacg ataagagaga atttttttca aaaaatgatc ttgagcaatt tttttcaatt gagaagatca ataaatctcc tgctattttt gattatcata aattggattt tttcaatagc tactatatta gagaaaaaaa agatgaagat ttatttaatc ttttactccc ttttttccaa aaaaaagggt atgtttctaa gcctagtact ttggaagaaa atcaaaaatt aaagttatta attcctctta taaagagtag aattaaaaaa ttaagtgatg ctttaaatat gactaaattt ttttatgagg acattaaatc ttggaattta gatgagtttt taagtagaaa aaaaacagct aaagaagttt gttctatttt agaattaata aagcctattt tagaagggtt tgaaaaaaga tcatcagaag aaaatgataa aattttttat gattttgctg agagtaatgg ttttaaattg ggagaaattc ttcttcctat tagaattgca gcgcttggta gcaaagtctc tccgccgctt tttgattctt taaaattgat aggcaagtct aaagtttttg aaagaataaa attagcacag gaatttttaa gaataaatga atagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BobuA.01348.a.A1.PW27582
| PURIFICATION DATe: | 9/22/2009 |
| CONCENTRATION: | 15mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 94 |
| PERCENT COVERAGE: | 38 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMX TLEAQTQGPG SMLSTRVRYA PSPTGLQHIG GIRTALFNYF FAKSCGGKFL LRIEDTDQSR YSPEAENDLY SSLKWLGISF DEGPVVGGDY APYVQSQRSA IYKQYAKYLI ESGHAYYXYC SPEXLERIKK IQNINKMPPG YDXHCRNLSX EEVEXALIKK IKPVVRFKIP LXGXTXFDDX LLGRI
Validated NT Sequence
ccatggctca tcaccatcac catcatatgn ntaccctgga agctcagacc cagggtcctg gttcgatgtt gagtacaaga gttcgttatg cgccttctcc aacgggttta caacatattg gcgggattag aacagctttg tttaattatt tttttgcaaa gtcttgcgga ggtaaatttt tgcttagaat tgaggataca gatcagagca ggtattctcc agaagctgaa aatgatcttt attcaagtct taaatggctt ggcatttctt ttgatgaagg ccctgttgta gggggtgatt atgcgcctta tgttcagtct caaagaagtg cnatatataa gcaatatgct aaatatttga ttgaatctgg gcatgcttat tattnttatt gcagtcccga anggcttgaa agaattaaga aaattcaaaa tattaataag atgccacctg gatatgatng gcattgcagg aatttaagta angaagaggt tgaganngca ctaattaaaa aaatcaagcc tgttgttaga tttaaaattc ctttanaagg nnnnaccnnn tttgatgatn ttttacttgg aaggatta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSTRVRYA PSPTGLQHIG GIRTALFNYF FAKSCGGKFL LRIEDTDQSR YSPEAENDLY SSLKWLGISF DEGPVVGGDY APYVQSQRSA IYKQYAKYLI ESGHAYYCYC SPERLERIKK IQNINKMPPG YDRHCRNLSN EEVENALIKK IKPVVRFKIP LEGDTSFDDI LLGRITWANK DISPDPVILK SDGLPTYHLA NVVDDYLMKI THVLRAQEWV SSGPLHVLLY KAFKWKPPIY CHLPMVMGND GQKLSKRHGS TALRQFIEDG YLPEAIINYV TLLGWSYDDK REFFSKNDLE QFFSIEKINK SPAIFDYHKL DFFNSYYIRE KKDEDLFNLL LPFFQKKGYV SKPSTLEENQ KLKLLIPLIK SRIKKLSDAL NMTKFFYEDI KSWNLDEFLS RKKTAKEVCS ILELIKPILE GFEKRSSEEN DKIFYDFAES NGFKLGEILL PIRIAALGSK VSPPLFDSLK LIGKSKVFER IKLAQEFLRI NE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGAGTACA AGAGTTCGTT ATGCGCCTTC TCCAACGGGT TTACAACATA TTGGCGGGAT TAGAACAGCT TTGTTTAATT ATTTTTTTGC AAAGTCTTGC GGAGGTAAAT TTTTGCTTAG AATTGAGGAT ACAGATCAGA GCAGGTATTC TCCAGAAGCT GAAAATGATC TTTATTCAAG TCTTAAATGG CTTGGCATTT CTTTTGATGA AGGCCCTGTT GTAGGGGGTG ATTATGCGCC TTATGTTCAG TCTCAAAGAA GTGCAATATA TAAGCAATAT GCTAAATATT TGATTGAATC TGGGCATGCT TATTATTGTT ATTGCAGTCC CGAAAGGCTT GAAAGAATTA AGAAAATTCA AAATATTAAT AAGATGCCAC CTGGATATGA TAGGCATTGC AGGAATTTAA GTAATGAAGA GGTTGAGAAT GCACTAATTA AAAAAATCAA GCCTGTTGTT AGATTTAAAA TTCCTTTAGA AGGAGATACC AGCTTTGATG ATATTTTACT TGGAAGGATT ACATGGGCGA ATAAAGACAT TAGTCCTGAT CCTGTAATTC TTAAGTCAGA TGGATTGCCG ACTTATCATC TTGCCAATGT TGTTGATGAT TATTTAATGA AAATTACCCA TGTATTAAGG GCTCAAGAAT GGGTTTCTTC AGGTCCATTG CACGTACTTC TTTATAAGGC TTTTAAATGG AAACCCCCTA TTTATTGTCA CCTTCCAATG GTTATGGGAA ATGATGGTCA AAAATTAAGC AAAAGACATG GCTCAACAGC TTTAAGACAG TTTATTGAAG ATGGGTATCT TCCAGAGGCT ATTATTAATT ATGTTACTTT GCTTGGCTGG TCTTACGACG ATAAGAGAGA ATTTTTTTCA AAAAATGATC TTGAGCAATT TTTTTCAATT GAGAAGATCA ATAAATCTCC TGCTATTTTT GATTATCATA AATTGGATTT TTTCAATAGC TACTATATTA GAGAAAAAAA AGATGAAGAT TTATTTAATC TTTTACTCCC TTTTTTCCAA AAAAAAGGGT ATGTTTCTAA GCCTAGTACT TTGGAAGAAA ATCAAAAATT AAAGTTATTA ATTCCTCTTA TAAAGAGTAG AATTAAAAAA TTAAGTGATG CTTTAAATAT GACTAAATTT TTTTATGAGG ACATTAAATC TTGGAATTTA GATGAGTTTT TAAGTAGAAA AAAAACAGCT AAAGAAGTTT GTTCTATTTT AGAATTAATA AAGCCTATTT TAGAAGGGTT TGAAAAAAGA TCATCAGAAG AAAATGATAA AATTTTTTAT GATTTTGCTG AGAGTAATGG TTTTAAATTG GGAGAAATTC TTCTTCCTAT TAGAATTGCA GCGCTTGGTA GCAAAGTCTC TCCGCCGCTT TTTGATTCTT TAAAATTGAT AGGCAAGTCT AAAGTTTTTG AAAGAATAAA ATTAGCACAG GAATTTTTAA GAATAAATGA ATAGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA