BobuA.01301.a

phosphoribosylpyrophosphate synthetase (BB_0544)

CENTER ID:	BobuA.01301.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01301.a.A1.GE26609	Full length( BobuA.01301.a )		1	406

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126730
PATRIC ID:	fig\|224326.49.peg.935
RefSeq:	NP_212678.1
UniProt:	O51494

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNLLKKKSIG IIACPGGRVF ASKIIEELDR IFINIENEII ESICQDSKAL KEEIFKIEKV LSPFLEGLSL STLKSKEPLE IPVKFVKFAN GEFKTEILKT IRNKDIFIVQ DVANAYEVEI NSSEKIIMTV NDHIMNLMTT IDACMQAKAN SVSVIIPSYP YSRQDKKHSR ECLTASLIGR FLEELGIRHI LTLDIHSKAI ENVFRKVYFE NLNVSYEIFN SLKDLIDIRD SNLVIVSPDT GAVSRNKFFA SSLKSPLALL YKERDYSRVS NDVADSNISV TKLLGDVEGK NVFMSDDMLA TGGTLIKAMK LLKSMGAKKI ICGISLPFFN GDAIKYFDKA YEEGFFYKII GTNAVCHNDE LINKPWYHEA NVAPLFAEAI FAIYNKVGLQ KILDRKDDIQ KLITKG

NT Sequence

GTGAATTTAC TTAAAAAGAA ATCAATAGGA ATTATTGCTT GTCCTGGGGG TAGGGTTTTT GCTAGTAAAA TAATAGAAGA GCTTGACAGA ATATTTATAA ATATTGAAAA TGAGATTATA GAGAGCATAT GCCAAGATTC TAAAGCCTTA AAGGAAGAAA TTTTTAAGAT TGAAAAAGTT TTATCTCCTT TTTTAGAAGG ACTTAGTTTG TCTACTCTTA AAAGCAAAGA GCCTTTAGAA ATTCCTGTAA AATTTGTTAA ATTTGCCAAT GGTGAATTTA AAACAGAAAT TTTAAAAACA ATCAGAAATA AGGATATTTT TATTGTTCAA GATGTTGCTA ATGCTTATGA AGTTGAGATA AACAGTAGCG AAAAAATAAT TATGACGGTT AATGATCACA TAATGAATTT AATGACAACA ATAGATGCCT GTATGCAGGC CAAAGCCAAT TCTGTTAGTG TTATTATCCC GTCTTATCCT TATTCAAGAC AAGATAAAAA GCATTCAAGA GAATGCTTAA CAGCAAGTCT TATTGGAAGA TTTTTAGAAG AGTTGGGCAT TAGACATATT TTAACCCTAG ATATTCACTC AAAGGCTATT GAGAATGTCT TTAGGAAAGT TTATTTTGAA AATTTAAATG TTTCTTATGA AATCTTTAAT TCTCTTAAGG ATTTAATCGA CATTAGAGAT TCTAATTTAG TTATTGTTTC ACCCGATACA GGTGCTGTAA GTAGAAATAA ATTTTTTGCA TCAAGCCTTA AGAGCCCTCT TGCTTTGCTT TATAAGGAGA GAGATTATTC AAGAGTTTCA AATGATGTTG CTGATTCAAA TATTTCTGTA ACCAAGCTTT TAGGAGATGT CGAAGGCAAG AATGTTTTTA TGAGTGACGA CATGTTAGCT ACTGGGGGCA CTCTAATTAA GGCAATGAAA TTACTTAAAA GCATGGGTGC TAAGAAGATT ATATGTGGGA TTAGTTTGCC GTTTTTCAAT GGAGATGCTA TTAAATATTT TGATAAAGCT TATGAGGAGG GATTTTTTTA TAAAATAATT GGAACAAATG CTGTTTGTCA TAATGATGAA TTAATAAATA AACCTTGGTA TCATGAGGCT AATGTTGCGC CTCTTTTTGC GGAGGCAATA TTTGCAATTT ATAATAAAGT TGGTTTGCAA AAAATTCTTG ATAGAAAGGA TGATATTCAA AAATTGATTA CCAAGGGT

Details for BobuA.01301.a.A1.GE26609

HARVESTED ON:	4/23/2009
SEQUENCED ON:	5/21/2009
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	24

Validated AA Sequence

MSDXMLATGG TLIKAMKLLK SMGAKKIICG ISXPFFXGDA IKYFDKAYEE GFFYKIIGTN AVCHNDELIN KPWYHEANVA PLFXEAIFAI YNKVGLQKIX DRK

Validated NT Sequence

cctttctatc angaattttt tgcaaaccaa ctttattata aattgcaaat attgcctcnn caaaaagagg cgcaacatta gcctcatgat accaaggttt atttattaat tcatcattat gacaaacagc atttgttcca attattttat aaaaaaatcc ctcctcataa gctttatcaa aatatttaat agcatctccn ttgaaaaacg gcnaactaat cccacatata atcttcttag cacccatgct tttaagtaat ttcattgcct taattagagt gcccccagta gctaacatgt ngtcactcat aaaaacattc ttgccttcga catctcctaa aagcttggtt acngaaatan ttgaatcagc aacatcattt gaaactcttg aataatctct ctccttataa agcaaagcaa ganggctctn aangcttgat gcaaaaaatt tatttctact tacagcacnn gtatcgggng aaanaannac taaattnnaa tcnctnnnnn cnnttaaatc cttaaganaa ttaangatct canannaaac atttaaattt tcaaaatana ctttcctnaa gacattcnca nnnnccnttg agtgnatanc nnnnnntana atatgnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVNLLKKKS IGIIACPGGR VFASKIIEEL DRIFINIENE IIESICQDSK ALKEEIFKIE KVLSPFLEGL SLSTLKSKEP LEIPVKFVKF ANGEFKTEIL KTIRNKDIFI VQDVANAYEV EINSSEKIIM TVNDHIMNLM TTIDACMQAK ANSVSVIIPS YPYSRQDKKH SRECLTASLI GRFLEELGIR HILTLDIHSK AIENVFRKVY FENLNVSYEI FNSLKDLIDI RDSNLVIVSP DTGAVSRNKF FASSLKSPLA LLYKERDYSR VSNDVADSNI SVTKLLGDVE GKNVFMSDDM LATGGTLIKA MKLLKSMGAK KIICGISLPF FNGDAIKYFD KAYEEGFFYK IIGTNAVCHN DELINKPWYH EANVAPLFAE AIFAIYNKVG LQKILDRKDD IQKLITKG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaattta cttaaaaaga aatcaatagg aattattgct tgtcctgggg gtagggtttt tgctagtaaa ataatagaag agcttgacag aatatttata aatattgaaa atgagattat agagagcata tgccaagatt ctaaagcctt aaaggaagaa atttttaaga ttgaaaaagt tttatctcct tttttagaag gacttagttt gtctactctt aaaagcaaag agcctttaga aattcctgta aaatttgtta aatttgccaa tggtgaattt aaaacagaaa ttttaaaaac aatcagaaat aaggatattt ttattgttca agatgttgct aatgcttatg aagttgagat aaacagtagc gaaaaaataa ttatgacggt taatgatcac ataatgaatt taatgacaac aatagatgcc tgtatgcagg ccaaagccaa ttctgttagt gttattatcc cgtcttatcc ttattcaaga caagataaaa agcattcaag agaatgctta acagcaagtc ttattggaag atttttagaa gagttgggca ttagacatat tttaacccta gatattcact caaaggctat tgagaatgtc tttaggaaag tttattttga aaatttaaat gtttcttatg aaatctttaa ttctcttaag gatttaatcg acattagaga ttctaattta gttattgttt cacccgatac aggtgctgta agtagaaata aattttttgc atcaagcctt aagagccctc ttgctttgct ttataaggag agagattatt caagagtttc aaatgatgtt gctgattcaa atatttctgt aaccaagctt ttaggagatg tcgaaggcaa gaatgttttt atgagtgacg acatgttagc tactgggggc actctaatta aggcaatgaa attacttaaa agcatgggtg ctaagaagat tatatgtggg attagtttgc cgtttttcaa tggagatgct attaaatatt ttgataaagc ttatgaggag ggattttttt ataaaataat tggaacaaat gctgtttgtc ataatgatga attaataaat aaaccttggt atcatgaggc taatgttgcg cctctttttg cggaggcaat atttgcaatt tataataaag ttggtttgca aaaaattctt gatagaaagg atgatattca aaaattgatt accaagggtt aaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa