BobuA.01293.a

aminotransferase (nifS) (BB_0084)

CENTER ID:	BobuA.01293.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01293.a.A1.GE26607	Full length( BobuA.01293.a )		1	422

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_128800
PATRIC ID:	fig\|224326.49.peg.482
RefSeq:	NP_212218.1
UniProt:	O51111

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDFKQIKSNV EKVKFLRKDF PILNKKFDNK YIIYFDNAAT SQKPKNVIYS NVEYYENYNA NVHRSGHKFA IQSSIKIEKT RELVKNFINA ESAKNIIFTS GTTDGINTIA SSFFYSKYFK KKDEIILTTL EHNSNLLPWV NLANLANLKI KLAKFNEMGI ITPEEIEKLI TEKTKLISIS GINNTLGTIN DLESIGKIAK KYNICLFVDA AQMAPHIKID VKKIGCDFLV FSGHKMLAPT GIGILYISNN MAEKLHSSKL GGNTVEEIFI ENEKIKFKAS DSPNKFESGT PNIAGIIGLE EAIKYIDNIS MDFILEHDKQ LIEYGVKKLQ ELDEVEFILN TNLKRNSIIS FTVKNIHSHD IETYLDTMGI ATRAGRTCSY VAFFPENLNK DHLLRISFYF YNTQEEIDNF ILGLKKVIKE LS

NT Sequence

ATGGATTTCA AACAAATAAA AAGCAATGTC GAAAAGGTTA AATTTTTAAG AAAAGATTTT CCTATTTTAA ATAAAAAATT TGACAATAAG TATATAATTT ACTTTGATAA TGCAGCAACC TCTCAAAAGC CCAAAAACGT AATTTATTCT AACGTTGAAT ATTATGAAAA TTACAACGCA AATGTACACA GAAGCGGTCA TAAATTTGCA ATTCAATCGA GCATAAAAAT AGAAAAAACA AGAGAACTTG TAAAAAATTT CATTAATGCA GAATCTGCAA AAAATATAAT ATTCACCTCT GGAACTACTG ATGGAATTAA TACCATTGCA AGTTCATTTT TTTATTCAAA ATACTTTAAA AAAAAAGATG AAATTATTCT TACAACTCTT GAACATAACA GCAATTTACT GCCTTGGGTA AATCTTGCAA ATTTAGCTAA TTTAAAAATT AAATTAGCTA AATTCAATGA AATGGGAATT ATTACCCCTG AAGAAATTGA AAAACTTATT ACAGAAAAAA CAAAGCTCAT CAGTATTTCA GGAATAAATA ATACCCTAGG AACCATTAAT GATTTGGAAT CTATTGGAAA AATCGCAAAA AAATACAATA TATGTCTCTT TGTAGATGCT GCACAAATGG CACCTCATAT AAAAATAGAT GTTAAAAAAA TTGGCTGTGA TTTTTTGGTA TTTTCTGGAC ATAAAATGCT TGCTCCAACA GGAATAGGAA TTTTATATAT TTCAAATAAT ATGGCTGAAA AACTTCACAG CTCAAAATTG GGGGGAAATA CTGTAGAAGA AATATTTATA GAAAATGAAA AAATTAAATT TAAAGCATCT GATTCTCCTA ATAAATTTGA ATCGGGAACC CCAAATATTG CAGGAATTAT TGGACTTGAA GAGGCAATAA AATATATTGA CAATATTTCT ATGGATTTTA TTTTAGAGCA TGATAAACAG TTAATCGAAT ATGGGGTAAA AAAATTACAA GAGCTTGATG AAGTTGAATT TATACTAAAT ACAAATCTTA AAAGAAATTC AATAATATCA TTTACAGTAA AAAATATCCA CTCTCACGAT ATTGAAACAT ATCTAGATAC AATGGGAATA GCAACCAGAG CCGGAAGAAC TTGTTCCTAT GTGGCATTTT TCCCAGAAAA TTTAAATAAA GACCATCTTT TAAGAATTAG CTTTTATTTT TATAATACAC AAGAAGAAAT TGATAATTTC ATATTGGGAT TAAAAAAGGT AATAAAAGAG CTTTCA

Details for BobuA.01293.a.A1.GE26607

HARVESTED ON:	4/23/2009
SEQUENCED ON:	5/21/2009
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	92

Validated AA Sequence

Validated NT Sequence

atgaaattat caatttcttc ttgtgtatta taaaaataaa agctaattct taaaagatgg tctttattta aattttctgg gaaaaatgcc acataggaac aagttcttcc ggctctggtt gctattccca ttgtatctag atatgtttca atatcgtgag agtggatatt ttttactgta aatgatatta ttgaatttct tttaagattt gtatttagta taaattcaac ttcatcaagc tcttgtaatt tttttacccc atattcgatt aactgtttat catgctctaa aataaaatcc atagaaatat tgtcaatata ttttattgcc tcttcaagtc caataattcc tgcaatattt ggggttcccg attcaaattt attaggagaa tcagatgctt taaatttaat tttttcattt tctataaata tttcttctac ngtatttccc cccaattttg agctgtgaag tttttcagcc atattatttg aaatatataa aattcctatt cctgttggag caagcatttt atgtccagaa aataccaaaa aatcacagcc aattttttta acatctattt ttatatgagg tgccatttgt gcagcatcta caaagagaca tatattgtat ttttttgcga tttttccaat agattccaaa tcattaatgg ttcctagggt attatttatt cctgaaatac tgatgagctt tgttttttct gtaataagtt tttcaatttc ttcaggggta ataattccca tttcattgaa tttagctaat ttaattttta aattagctaa atttgcaaga tttacccaag gcagtaaatt gctgttatgt tcaagagttg taagaataat ttcatctttt tttttaaagt attttgaata aaaaaatgaa cttgcaatgg tattaattcc atcagtagtt ccagaggtga atattatatt ttttgcagat tctgcattaa tgaaattttt tacaagttct cttgtttttt ctatttttat gctcgattga attgcaaatt tatgaccgct tctgtgtaca tttgcgttgt aattttcata atattcaacg ttagaataaa ttacgttttt gggcttttga gaggttgctg cattatcaaa gtaaattata tacttattgt caaatttttt atttaaaata ggaaaatctt ttcttaaaaa tttaaccttt tcgacattgc tttttatttg tttgaaatcc atcgaaccag gaccctgggt ctgagctt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDFKQIKSN VEKVKFLRKD FPILNKKFDN KYIIYFDNAA TSQKPKNVIY SNVEYYENYN ANVHRSGHKF AIQSSIKIEK TRELVKNFIN AESAKNIIFT SGTTDGINTI ASSFFYSKYF KKKDEIILTT LEHNSNLLPW VNLANLANLK IKLAKFNEMG IITPEEIEKL ITEKTKLISI SGINNTLGTI NDLESIGKIA KKYNICLFVD AAQMAPHIKI DVKKIGCDFL VFSGHKMLAP TGIGILYISN NMAEKLHSSK LGGNTVEEIF IENEKIKFKA SDSPNKFESG TPNIAGIIGL EEAIKYIDNI SMDFILEHDK QLIEYGVKKL QELDEVEFIL NTNLKRNSII SFTVKNIHSH DIETYLDTMG IATRAGRTCS YVAFFPENLN KDHLLRISFY FYNTQEEIDN FILGLKKVIK ELS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatttcaaa caaataaaaa gcaatgtcga aaaggttaaa tttttaagaa aagattttcc tattttaaat aaaaaatttg acaataagta tataatttac tttgataatg cagcaacctc tcaaaagccc aaaaacgtaa tttattctaa cgttgaatat tatgaaaatt acaacgcaaa tgtacacaga agcggtcata aatttgcaat tcaatcgagc ataaaaatag aaaaaacaag agaacttgta aaaaatttca ttaatgcaga atctgcaaaa aatataatat tcacctctgg aactactgat ggaattaata ccattgcaag ttcatttttt tattcaaaat actttaaaaa aaaagatgaa attattctta caactcttga acataacagc aatttactgc cttgggtaaa tcttgcaaat ttagctaatt taaaaattaa attagctaaa ttcaatgaaa tgggaattat tacccctgaa gaaattgaaa aacttattac agaaaaaaca aagctcatca gtatttcagg aataaataat accctaggaa ccattaatga tttggaatct attggaaaaa tcgcaaaaaa atacaatata tgtctctttg tagatgctgc acaaatggca cctcatataa aaatagatgt taaaaaaatt ggctgtgatt ttttggtatt ttctggacat aaaatgcttg ctccaacagg aataggaatt ttatatattt caaataatat ggctgaaaaa cttcacagct caaaattggg gggaaatact gtagaagaaa tatttataga aaatgaaaaa attaaattta aagcatctga ttctcctaat aaatttgaat cgggaacccc aaatattgca ggaattattg gacttgaaga ggcaataaaa tatattgaca atatttctat ggattttatt ttagagcatg ataaacagtt aatcgaatat ggggtaaaaa aattacaaga gcttgatgaa gttgaattta tactaaatac aaatcttaaa agaaattcaa taatatcatt tacagtaaaa aatatccact ctcacgatat tgaaacatat ctagatacaa tgggaatagc aaccagagcc ggaagaactt gttcctatgt ggcatttttc ccagaaaatt taaataaaga ccatctttta agaattagct tttattttta taatacacaa gaagaaattg ataatttcat attgggatta aaaaaggtaa taaaagagct ttcataaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa