BobuA.01244.a

6-phosphogluconolactonase (6PGL) (EC 3.1.1.31) (BB_0222)

CENTER ID:	BobuA.01244.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01244.a.B1.GE38613	full length		1	235

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|224326.49.peg.620
RefSeq:	NP_212356.1
UniProt:	O51240

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEFLYSDEEN YLKDRFFDFF NMNVDKDKYT SIGICGGRSI VNFLSVFLKQ NFSFRRSHFF LVDERCVPLN DENSNYNLLN KNFFSKMVDK NLISISKFHA FVYSEIDEAT AIHDYNIEFN SRFNIFDFII VSVGEDGHIA SLFPSRKLLF SDVEGYQYEY NSPKFPSKRI SLTPKSLFGS KAVVLLFMGV DKKCALENFL ASNSSINECP ARLLKEHPNL LVLTNIKRDE SYAGS

NT Sequence

atggagtttt tatattctga tgaagagaat tatttaaaag atagattttt tgattttttt aatatgaatg tagataaaga taaatataca agtattggaa tttgtggtgg tcgaagtata gttaactttt taagcgtttt tcttaaacaa aatttttctt ttagaagatc gcattttttt ttagttgatg aacggtgtgt tccattaaat gatgagaata gtaattataa tcttttaaat aaaaattttt tttctaaaat ggttgataaa aatttaataa gcatttccaa atttcatgct tttgtttata gcgaaataga cgaagccaca gctattcatg actataatat tgaatttaat tctaggttta atatttttga ttttattata gtttctgttg gtgaggatgg ccacatagca tcgctttttc cttcaagaaa attgttgttt tcagatgtag aaggttatca gtatgaatac aattctccca aattcccaag caaaagaata agcttaacgc cgaaatcttt gtttggttca aaagctgttg ttttgttatt tatgggtgtt gataaaaagt gtgctttaga aaatttttta gcttctaata gttctattaa tgaatgtcca gccagacttc tcaaagagca tcccaattta ctagttctta caaacattaa aagagatgaa agttatgcag gatcctag

Details for BobuA.01244.a.B1.GE38613

HARVESTED ON:	11/19/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHHM EFLYSDEENY LKDRFFDFFN MNVDKDKYTS IGICGGRSIV NFLSVFLKQN FSFRRSHFFL VDERCVPLND ENSNYNLLNK NFFSKMVDKN LISISKFHAF VYSEIDEATA IHDYNIEFNS RFNIFDFIIV SVGEDGHIAS LFPSRKLLFS DVEGYQYEYN SPKFPSKRIS LTPKSLFGSK AVVLLFMGVD KKCALENFLA SNSSINECPA RLLKEHPNLL VLTNIKRDES YAGS

Validated NT Sequence

atcttactca cttaggatcc tgcataactt tcatctcttt taatgtttgt aagaactagt aaattgggat gctctttgag aagtctggct ggacattcat taatagaact attagaagct aaaaaatttt ctaaagcaca ctttttatca acacccataa ataacaaaac aacagctttt gaaccaaaca aagatttcgg cgttaagctt attcttttgc ttgggaattt gggagaattg tattcatact gataaccttc tacatctgaa aacaacaatt ttcttgaagg aaaaagcgat gctatgtggc catcctcacc aacagaaact ataataaaat caaaaatatt aaacctagaa ttaaattcaa tattatagtc atgaatagct gtggcttcgt ctatttcgct ataaacaaaa gcatgaaatt tggaaatgct tattaaattt ttatcaacca ttttagaaaa aaaattttta tttaaaagat tataattact attctcatca tttaatggaa cacaccgttc atcaactaaa aaaaaatgcg atcttctaaa agaaaaattt tgtttaagaa aaacgcttaa aaagttaact atacttcgac caccacaaat tccaatactt gtatatttat ctttatctac attcatatta aaaaaatcaa aaaatctatc ttttaaataa ttctcttcat cagaatataa aaactccata tggtggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHME FLYSDEENYL KDRFFDFFNM NVDKDKYTSI GICGGRSIVN FLSVFLKQNF SFRRSHFFLV DERCVPLNDE NSNYNLLNKN FFSKMVDKNL ISISKFHAFV YSEIDEATAI HDYNIEFNSR FNIFDFIIVS VGEDGHIASL FPSRKLLFSD VEGYQYEYNS PKFPSKRISL TPKSLFGSKA VVLLFMGVDK KCALENFLAS NSSINECPAR LLKEHPNLLV LTNIKRDESY AGS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggagt ttttatattc tgatgaagag aattatttaa aagatagatt ttttgatttt tttaatatga atgtagataa agataaatat acaagtattg gaatttgtgg tggtcgaagt atagttaact ttttaagcgt ttttcttaaa caaaattttt cttttagaag atcgcatttt tttttagttg atgaacggtg tgttccatta aatgatgaga atagtaatta taatctttta aataaaaatt ttttttctaa aatggttgat aaaaatttaa taagcatttc caaatttcat gcttttgttt atagcgaaat agacgaagcc acagctattc atgactataa tattgaattt aattctaggt ttaatatttt tgattttatt atagtttctg ttggtgagga tggccacata gcatcgcttt ttccttcaag aaaattgttg ttttcagatg tagaaggtta tcagtatgaa tacaattctc ccaaattccc aagcaaaaga ataagcttaa cgccgaaatc tttgtttggt tcaaaagctg ttgttttgtt atttatgggt gttgataaaa agtgtgcttt agaaaatttt ttagcttcta atagttctat taatgaatgt ccagccagac ttctcaaaga gcatcccaat ttactagttc ttacaaacat taaaagagat gaaagttatg caggatcctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat