SSGCID: Target Page

BobuA.01237.a

recombinase A

CENTER ID:	BobuA.01237.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01237.a.A1.GE26486	Full length( BobuA.01237.a )		1	365

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.529
RefSeq:	NP_212265.1
UniProt:	Q59180

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKLKEKREK AVVGIERASK EEAIELARVQ IEKAFGKGSL IKMGESPVGQ GIKSMSSGSI VLDEALGIGG YPRGRIIEIF GPESSGKTTL TLQAIAEVQK EGGIAAFIDA EHALDPVYAK ALGVNVAELW LSQPDTGEQA LEIAEHLIRS GGVDLIVVDS VAALTPKLEI DGEMGDSQIG LQARLMSKAL RKITGILSKS NTCIMFINQI RMRIGVMFGN PETTTGGNAL KFYSSLRLEV RKIEQVTRSG SSDDVIGNKI RVKIVKNKVA PPFRKVELII YFGKGISREA GILDAAIKHN LIQKTGSWYS LGDNKLGQGR ESVIEYLSKE VELANNLDKR LRKIIFNNFD QENDNFIEFK EDESE

NT Sequence

ATGTCAAAGT TAAAGGAAAA AAGAGAAAAA GCTGTTGTTG GCATAGAAAG GGCAAGTAAA GAGGAAGCTA TTGAGCTTGC AAGAGTTCAA ATAGAAAAAG CTTTTGGAAA GGGAAGTCTT ATTAAGATGG GGGAATCTCC TGTTGGACAA GGTATAAAAA GTATGTCAAG TGGATCTATT GTATTAGATG AGGCTCTCGG CATTGGCGGA TATCCTAGGG GGCGCATAAT AGAAATTTTT GGCCCCGAGT CGTCTGGCAA GACTACTTTA ACTCTTCAAG CGATTGCTGA GGTGCAAAAA GAAGGTGGGA TAGCTGCTTT TATTGATGCT GAGCATGCTC TTGATCCTGT TTATGCAAAA GCTTTAGGTG TTAATGTTGC AGAACTTTGG CTTAGTCAGC CTGATACCGG AGAGCAAGCT CTTGAGATTG CTGAGCATTT AATCAGAAGT GGTGGTGTTG ATTTGATTGT AGTTGATTCT GTAGCGGCTT TAACCCCTAA ATTAGAGATA GATGGAGAAA TGGGAGATTC TCAGATTGGT CTTCAAGCAA GGCTAATGAG CAAAGCTCTT AGAAAGATTA CCGGTATACT TTCTAAATCC AATACTTGCA TTATGTTTAT TAATCAAATA AGGATGAGGA TTGGTGTTAT GTTTGGCAAT CCTGAGACCA CTACCGGTGG GAATGCTTTA AAATTTTATT CATCTCTTAG ACTTGAGGTT CGAAAGATCG AACAAGTAAC TAGATCGGGC TCAAGCGATG ATGTTATTGG CAATAAGATT AGAGTTAAGA TTGTAAAGAA CAAGGTTGCT CCACCTTTTC GTAAAGTAGA ATTGATAATT TATTTTGGGA AAGGTATTTC AAGAGAAGCT GGCATTTTAG ATGCTGCTAT TAAGCATAAT TTAATACAAA AAACAGGCTC ATGGTATTCA TTGGGAGATA ATAAGTTGGG ACAGGGGAGA GAGAGTGTTA TTGAGTATCT TAGCAAAGAA GTAGAACTTG CAAATAATTT GGATAAGAGG CTTAGAAAAA TAATTTTTAA TAATTTTGAT CAAGAGAATG ATAATTTTAT TGAATTTAAA GAAGATGAAT CTGAG

Details for BobuA.01237.a.A1.GE26486

HARVESTED ON:	4/20/2009
SEQUENCED ON:	5/6/2009
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSKLKEKRE KAVVGIERAS KEEAIELARV QIEKAFGKGS LIKMGESPVG QGIKSMSSGS IVLDEALGIG GYPRGRIIEI FGPESSGKTT LTLQAIAEVQ KEGGIAAFID AEHALDPVYA KALGVNVAEL WLSQPDTGEQ ALEIAEHLIR SGGVDLIVVD SVAALTPKLE IDGEMGDSQI GLQARLMSKA LRKITGILSK SNTCIMFINQ IRMRIGVMFG NPETTTGGNA LKFYSSLRLE VRKIEQVTRS GSSDDVIGNK IRVKIVKNKV APPFRKVELI IYFGKGISRE AGILDAAIKH NLIQKTGSWY SLGDNKLGQG RESVIEYLSK EVELANNLDK RLRKIIFNNF DQENDNFIEF KEDESE

Validated NT Sequence

ttattactca gattcatctt ctttaaattc aataaaatta tcattctctt gatcaaaatt attaaaaatt atttttctaa gcctcttatc caaattattt gcaagttcta cttctttgct aagatactca ataacactct ctctcccctg tcccaactta ttatctccca atgaatacca tgagcctgtt ttttgtatta aattatgctt aatagcagca tctaaaatgc cagcttctct tgaaatacct ttcccaaaat aaattatcaa ttctacttta cgaaaaggtg gagcaacctt gttctttaca atcttaactc taatcttatt gccaataaca tcatcgcttg agcccgatct agttacttgt tcgatctttc gaacctcaag tctaagagat gaataaaatt ttaaagcatt cccaccggta gtggtctcag gattgccaaa cataacacca atcctcatcc ttatttgatt aataaacata atgcaagtat tggatttaga aagtataccg gtaatctttc taagagcttt gctcattagc cttgcttgaa gaccaatctg agaatctccc atttctccat ctatctctaa tttaggggtt aaagccgcta cagaatcaac tacaatcaaa tcaacaccac cacttctgat taaatgctca gcaatctcaa gagcttgctc tccggtatca ggctgactaa gccaaagttc tgcaacatta acacctaaag cttttgcata aacaggatca agagcatgct cagcatcaat aaaagcagct atcccacctt ctttttgcac ctcagcaatc gcttgaagag ttaaagtagt cttgccagac gactcggggc caaaaatttc tattatgcgc cccctaggat atccgccaat gccgagagcc tcatctaata caatagatcc acttgacata ctttttatac cttgtccaac aggagattcc cccatcttaa taagacttcc ctttccaaaa gctttttcta tttgaactct tgcaagctca atagcttcct ctttacttgc cctttctatg ccaacaacag ctttttctct tttttccttt aactttgaca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccatggtatn tctcctt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcaaagtta aaggaaaaaa gagaaaaagc tgttgttggc atagaaaggg caagtaaaga ggaagctatt gagcttgcaa gagttcaaat agaaaaagct tttggaaagg gaagtcttat taagatgggg gaatctcctg ttggacaagg tataaaaagt atgtcaagtg gatctattgt attagatgag gctctcggca ttggcggata tcctaggggg cgcataatag aaatttttgg ccccgagtcg tctggcaaga ctactttaac tcttcaagcg attgctgagg tgcaaaaaga aggtgggata gctgctttta ttgatgctga gcatgctctt gatcctgttt atgcaaaagc tttaggtgtt aatgttgcag aactttggct tagtcagcct gataccggag agcaagctct tgagattgct gagcatttaa tcagaagtgg tggtgttgat ttgattgtag ttgattctgt agcggcttta acccctaaat tagagataga tggagaaatg ggagattctc agattggtct tcaagcaagg ctaatgagca aagctcttag aaagattacc ggtatacttt ctaaatccaa tacttgcatt atgtttatta atcaaataag gatgaggatt ggtgttatgt ttggcaatcc tgagaccact accggtggga atgctttaaa attttattca tctcttagac ttgaggttcg aaagatcgaa caagtaacta gatcgggctc aagcgatgat gttattggca ataagattag agttaagatt gtaaagaaca aggttgctcc accttttcgt aaagtagaat tgataattta ttttgggaaa ggtatttcaa gagaagctgg cattttagat gctgctatta agcataattt aatacaaaaa acaggctcat ggtattcatt gggagataat aagttgggac aggggagaga gagtgttatt gagtatctta gcaaagaagt agaacttgca aataatttgg ataagaggct tagaaaaata atttttaata attttgatca agagaatgat aattttattg aatttaaaga agatgaatct gagtaaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa