BobuA.01197.a

adenylate kinase (adk) (BB_0417)

CENTER ID: BobuA.01197.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01197.a.A1.GE26479 Full length( BobuA.01197.a ) 1 211
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01197.a.A1.PS00261 Full length( BobuA.01197.a ) 1 211
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126762
PATRIC ID: fig|224326.49.peg.811
RefSeq: NP_212551.1
UniProt: O51378
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGLVFLGPPG SGKGTISKII SNEFKYQHIS TGDLFRENIL NSTALGQEIK KIVERGELVP DLITIKIVED KIKAIKKNKD FILDGFPRNI CQAEALDKFL PNVKIINFLI DEELVIKRLS GRRICKSCNN IFNIYTLTTK KNGICDVCGG DLYQREDDKE ECLKTRLKEY KLQTKPLIEF YSKCSRLNNV DASVKIDEIK KKIIKIMLKK N
NT Sequence
ATGGGGCTTG TATTTTTAGG TCCTCCGGGT TCTGGAAAGG GGACTATTTC AAAAATTATT TCTAATGAAT TTAAATATCA ACACATTTCA ACAGGAGACT TATTTAGAGA AAATATTTTA AATTCTACGG CTCTTGGGCA AGAAATAAAA AAAATTGTTG AAAGGGGAGA ACTGGTCCCC GACCTAATTA CAATCAAAAT AGTAGAAGAT AAAATTAAAG CTATTAAAAA GAACAAAGAT TTTATTTTAG ATGGATTTCC GCGCAATATT TGTCAAGCCG AGGCTCTTGA CAAATTTTTG CCAAATGTAA AAATAATAAA TTTTTTAATT GATGAAGAGT TAGTGATAAA AAGACTCTCG GGAAGAAGAA TTTGCAAATC TTGCAATAAT ATTTTCAATA TATATACCCT AACAACAAAA AAAAATGGTA TTTGCGACGT TTGTGGGGGA GATCTTTATC AACGAGAAGA TGATAAAGAA GAATGCTTAA AAACAAGACT CAAAGAATAT AAATTACAAA CAAAACCGCT AATAGAATTT TACTCAAAAT GCTCTAGACT TAACAATGTT GATGCATCTG TTAAAATTGA TGAAATAAAA AAAAAGATAA TTAAAATAAT GTTAAAAAAA AAT
Details for BobuA.01197.a.A1.GE26479
HARVESTED ON: 4/20/2009
SEQUENCED ON: 5/6/2009
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGLVFLGPP GSGKGTISKI ISNEFKYQHI STGDLFRENI LNSTALGQEI KKIVERGELV PDLITIKIVE DKIKAIKKNK DFILDGFPRN ICQAEALDKF LPNVKIINFL IDEELVIKRL SGRRICKSCN NIFNIYTLTT KKNGICDVCG GDLYQREDDK EECLKTRLKE YKLQTKPLIE FYSKCSRLNN VDASVKIDEI KKKIIKIMLK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggggc ttgtattttt aggtcctccg ggttctggaa aggggactat ttcaaaaatt atttctaatg aatttaaata tcaacacatt tcaacaggag acttatttag agaaaatatt ttaaattcta cggctcttgg gcaagaaata aaaaaaattg ttgaaagggg agaactggtc cccgacctaa ttacaatcaa aatagtagaa gataaaatta aagctattaa aaagaacaaa gattttattt tagatggatt tccgcgcaat atttgtcaag ccgaggctct tgacaaattt ttgccaaatg taaaaataat aaatttttta attgatgaag agttagtgat aaaaagactc tcgggaagaa gaatttgcaa atcttgcaat aatattttca atatatatac cctaacaaca aaaaaaaatg gtatttgcga cgtttgtggg ggagatcttt atcaacgaga agatgataaa gaagaatgct taaaaacaag actcaaagaa tataaattac aaacaaaacc gctaatagaa ttttactcaa aatgctctag acttaacaat gttgatgcat ctgttaaaat tgatgaaata aaaaaaaaga taattaaaat aatgttaaaa a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGLVFLGPP GSGKGTISKI ISNEFKYQHI STGDLFRENI LNSTALGQEI KKIVERGELV PDLITIKIVE DKIKAIKKNK DFILDGFPRN ICQAEALDKF LPNVKIINFL IDEELVIKRL SGRRICKSCN NIFNIYTLTT KKNGICDVCG GDLYQREDDK EECLKTRLKE YKLQTKPLIE FYSKCSRLNN VDASVKIDEI KKKIIKIMLK KN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggggcttgta tttttaggtc ctccgggttc tggaaagggg actatttcaa aaattatttc taatgaattt aaatatcaac acatttcaac aggagactta tttagagaaa atattttaaa ttctacggct cttgggcaag aaataaaaaa aattgttgaa aggggagaac tggtccccga cctaattaca atcaaaatag tagaagataa aattaaagct attaaaaaga acaaagattt tattttagat ggatttccgc gcaatatttg tcaagccgag gctcttgaca aatttttgcc aaatgtaaaa ataataaatt ttttaattga tgaagagtta gtgataaaaa gactctcggg aagaagaatt tgcaaatctt gcaataatat tttcaatata tataccctaa caacaaaaaa aaatggtatt tgcgacgttt gtgggggaga tctttatcaa cgagaagatg ataaagaaga atgcttaaaa acaagactca aagaatataa attacaaaca aaaccgctaa tagaatttta ctcaaaatgc tctagactta acaatgttga tgcatctgtt aaaattgatg aaataaaaaa aaagataatt aaaataatgt taaaaaaaaa ttaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BobuA.01197.a.A1.PS00261
PURIFICATION DATe: 6/17/2009
CONCENTRATION: 34.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGLVFLGPP GSGKGTISKI ISNEFKYQHI STGDLFRENI LNSTALGQEI KKIVERGELV PDLITIKIVE DKIKAIKKNK DFILDGFPRN ICQAEALDKF LPNVKIINFL IDEELVIKRL SGRRICKSCN NIFNIYTLTT KKNGICDVCG GDLYQREDDK EECLKTRLKE YKLQTKPLIE FYSKCSRLNN VDASVKIDEI KKKIIKIMLK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggggc ttgtattttt aggtcctccg ggttctggaa aggggactat ttcaaaaatt atttctaatg aatttaaata tcaacacatt tcaacaggag acttatttag agaaaatatt ttaaattcta cggctcttgg gcaagaaata aaaaaaattg ttgaaagggg agaactggtc cccgacctaa ttacaatcaa aatagtagaa gataaaatta aagctattaa aaagaacaaa gattttattt tagatggatt tccgcgcaat atttgtcaag ccgaggctct tgacaaattt ttgccaaatg taaaaataat aaatttttta attgatgaag agttagtgat aaaaagactc tcgggaagaa gaatttgcaa atcttgcaat aatattttca atatatatac cctaacaaca aaaaaaaatg gtatttgcga cgtttgtggg ggagatcttt atcaacgaga agatgataaa gaagaatgct taaaaacaag actcaaagaa tataaattac aaacaaaacc gctaatagaa ttttactcaa aatgctctag acttaacaat gttgatgcat ctgttaaaat tgatgaaata aaaaaaaaga taattaaaat aatgttaaaa a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGLVFLGPP GSGKGTISKI ISNEFKYQHI STGDLFRENI LNSTALGQEI KKIVERGELV PDLITIKIVE DKIKAIKKNK DFILDGFPRN ICQAEALDKF LPNVKIINFL IDEELVIKRL SGRRICKSCN NIFNIYTLTT KKNGICDVCG GDLYQREDDK EECLKTRLKE YKLQTKPLIE FYSKCSRLNN VDASVKIDEI KKKIIKIMLK KN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGGCTTGTA TTTTTAGGTC CTCCGGGTTC TGGAAAGGGG ACTATTTCAA AAATTATTTC TAATGAATTT AAATATCAAC ACATTTCAAC AGGAGACTTA TTTAGAGAAA ATATTTTAAA TTCTACGGCT CTTGGGCAAG AAATAAAAAA AATTGTTGAA AGGGGAGAAC TGGTCCCCGA CCTAATTACA ATCAAAATAG TAGAAGATAA AATTAAAGCT ATTAAAAAGA ACAAAGATTT TATTTTAGAT GGATTTCCGC GCAATATTTG TCAAGCCGAG GCTCTTGACA AATTTTTGCC AAATGTAAAA ATAATAAATT TTTTAATTGA TGAAGAGTTA GTGATAAAAA GACTCTCGGG AAGAAGAATT TGCAAATCTT GCAATAATAT TTTCAATATA TATACCCTAA CAACAAAAAA AAATGGTATT TGCGACGTTT GTGGGGGAGA TCTTTATCAA CGAGAAGATG ATAAAGAAGA ATGCTTAAAA ACAAGACTCA AAGAATATAA ATTACAAACA AAACCGCTAA TAGAATTTTA CTCAAAATGC TCTAGACTTA ACAATGTTGA TGCATCTGTT AAAATTGATG AAATAAAAAA AAAGATAATT AAAATAATGT TAAAAAAAAA TTAAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA