BobuA.01190.a

Phosphoglycolate phosphatase (BB_0676)

CENTER ID: BobuA.01190.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01190.a.B1.GE39071 full length 1 220
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1067
RefSeq: NP_212810.1
UniProt: O51619

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIKACIFDM DGTLVNSIMD IAFSMNSALS NLGYSKIELS KFNALVGRGF NKFVIDTLKL LSLEHDNPNL QEKLYKEFVK EYNKNLSFQT KPYENIKPLL ETMNKLNIPI GILSNKNHEE LINLVKNIFG NILFFEIRGY SKNFPPKPDP ENALDMILEL NAQKEEIAYI GDSDVDMLTA LNAGFMPIGV SWGFRSVQEL KESGAKHIIH NPLELLDLIK
NT Sequence
atgaaaatca aagcctgcat ttttgatatg gatggaacac tggtaaatag tataatggat attgcattct caatgaattc tgctctttca aacttaggat acagtaaaat agaactaagc aaattcaatg cccttgttgg cagaggattt aacaagtttg taatagacac tctaaagcta ttatctcttg aacatgataa tcctaattta caagaaaaac tttacaaaga atttgttaaa gaatacaata aaaacctttc attccaaaca aaaccatatg aaaatataaa acccctttta gaaactatga ataagcttaa cattccaatt ggaattttaa gcaataagaa ccacgaagaa ttaataaatt tggtgaaaaa tatttttgga aatatattgt tttttgaaat caggggttat tcaaaaaatt ttccaccaaa gccagatcct gaaaatgccc ttgatatgat attagaatta aatgcccaaa aagaagaaat tgcatatatt ggagacagcg atgtggatat gctaaccgca ctaaacgctg gatttatgcc aataggggtt tcttggggat ttagaagtgt tcaagaatta aaagaaagtg gagcaaaaca tataatacac aatccacttg aactattgga cctaataaaa tga
Details for BobuA.01190.a.B1.GE39071
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK IKACIFDMDG TLVNSIMDIA FSMNSALSNL GYSKIELSKF NALVGRGFNK FVIDTLKLLS LEHDNPNLQE KLYKEFVKEY NKNLSFQTKP YENIKPLLET MNKLNIPIGI LSNKNHEELI NLVKNIFGNI LFFEIRGYSK NFPPKPDPEN ALDMILELNA QNEEIAYIGD SDVDMLTALN AGFMPIGVSW GFRSVQELKE SGAKHIIHNP LELLDLIK
Validated NT Sequence
atcttactca cttattttat taggtccaat agttcaagtg gattgtgtat tatatgtttt gctccacttt cttttaattc ttgaacactt ctaaatcccc aagaaacccc tattggcata aatccagcgt ttagtgcggt tagcatatcc acatcgctgt ctccaatata tgcaatttct tcgttttggg catttaattc taatatcata tcaagggcat tttcaggatc tggctttggt ggaaaatttt ttgaataacc cctgatttca aaaaacaata tatttccaaa aatatttttc accaaattta ttaattcttc gtggttctta ttgcttaaaa ttccaattgg aatgttaagc ttattcatag tttctaaaag gggttttata ttttcatatg gttttgtttg gaatgaaagg tttttattgt attctttaac aaattctttg taaagttttt cttgtaaatt aggattatca tgttcaagag ataatagctt tagagtgtct attacaaact tgttaaatcc tctgccaaca agggcattga atttgcttag ttctatttta ctgtatccta agtttgaaag agcagaattc attgagaatg caatatccat tatactattt accagtgttc catccatatc aaaaatgcag gctttgattt tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMK IKACIFDMDG TLVNSIMDIA FSMNSALSNL GYSKIELSKF NALVGRGFNK FVIDTLKLLS LEHDNPNLQE KLYKEFVKEY NKNLSFQTKP YENIKPLLET MNKLNIPIGI LSNKNHEELI NLVKNIFGNI LFFEIRGYSK NFPPKPDPEN ALDMILELNA QKEEIAYIGD SDVDMLTALN AGFMPIGVSW GFRSVQELKE SGAKHIIHNP LELLDLIK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tcaaagcctg catttttgat atggatggaa cactggtaaa tagtataatg gatattgcat tctcaatgaa ttctgctctt tcaaacttag gatacagtaa aatagaacta agcaaattca atgcccttgt tggcagagga tttaacaagt ttgtaataga cactctaaag ctattatctc ttgaacatga taatcctaat ttacaagaaa aactttacaa agaatttgtt aaagaataca ataaaaacct ttcattccaa acaaaaccat atgaaaatat aaaacccctt ttagaaacta tgaataagct taacattcca attggaattt taagcaataa gaaccacgaa gaattaataa atttggtgaa aaatattttt ggaaatatat tgttttttga aatcaggggt tattcaaaaa attttccacc aaagccagat cctgaaaatg cccttgatat gatattagaa ttaaatgccc aaaaagaaga aattgcatat attggagaca gcgatgtgga tatgctaacc gcactaaacg ctggatttat gccaataggg gtttcttggg gatttagaag tgttcaagaa ttaaaagaaa gtggagcaaa acatataata cacaatccac ttgaactatt ggacctaata aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat