BobuA.01182.b

conserved hypothetical protein

CENTER ID: BobuA.01182.b
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01182.b.A1.GE26491 Full length( BobuA.01182.b ) 1 251
BobuA.01182.b.B1.GE27145 Full length( BobuA.01182.b ) 1 251
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1385
RefSeq: AAC65994.1
UniProt: O50684

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDIKKSDIIT MASIKGGVGK SVLSILFSYV LKELGKKVLL IDLDPQNSLT SYFNRYISNI EKYNTYSMLK GDFHFNECIK KIDDYISIIP SHPILGKFNS EAIDYKEVIL EHHLNENMQN YNFDYVLLDT PPSLDFLLKN ALNVADYIVI PVQVEIWSIE SFTILINAVN DITKFRKKIY NISIVENQFI KNRNTIKEVE DLLYKEYREY IKGKIHFSNS IKVLINGRLE PSKKEMYYRE IKDTLKNIFS L
NT Sequence
ATGGATATTA AAAAATCAGA TATTATAACA ATGGCTTCAA TTAAGGGAGG AGTCGGAAAA AGTGTGCTTT CTATACTTTT TTCTTATGTA TTAAAGGAAT TGGGCAAAAA GGTGCTTCTA ATTGATTTAG ATCCACAAAA TTCTTTAACT TCTTATTTTA ATAGATATAT TTCAAATATT GAAAAATATA ATACATATAG TATGTTAAAA GGAGATTTCC ATTTTAATGA ATGCATTAAA AAAATTGATG ATTATATATC TATAATCCCC TCTCACCCCA TTTTGGGAAA ATTTAATTCG GAAGCCATTG ATTACAAAGA AGTTATTTTA GAACATCATT TAAATGAAAA TATGCAAAAC TATAATTTTG ATTATGTTTT ATTAGATACT CCTCCTAGTT TAGATTTTCT TTTAAAGAAT GCCTTGAATG TTGCGGATTA TATTGTGATT CCAGTTCAGG TAGAAATATG GTCAATAGAA AGTTTTACTA TTTTGATTAA CGCAGTTAAT GATATTACAA AATTTAGAAA GAAAATATAT AATATTTCTA TTGTGGAAAA CCAGTTTATA AAAAATAGAA ACACGATAAA AGAAGTGGAG GACTTGCTTT ATAAAGAATA TAGAGAATAT ATTAAAGGCA AGATCCATTT TTCAAATAGT ATAAAAGTTC TTATAAATGG ACGATTAGAG CCCTCTAAAA AAGAAATGTA TTATAGAGAA ATAAAAGATA CTTTAAAAAA TATTTTTTCT TTA
Details for BobuA.01182.b.A1.GE26491
HARVESTED ON: 4/20/2009
SEQUENCED ON: 5/6/2009
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDIKKSDII TMASIKGGVG KSVLSILFSY VLKELGKKVL LIDLDPQNSL TSYFNRYISN IEKYNTYSML KGDFHFNECI KKIDDYISII PSHPILGKFN SEAIDYKEVI LEHHLNENMQ NYNFDYVLLD TPPSLDFLLK NALNVADYIV IPVQVEIWSI ESFTILINAV NDITKFRKKI YNISIVENQF IKNRNTIKEV EDLLYKEYRE YIKGKIHFSN SIKVLINGRL EPSKKEMYYR EIKDTLKNIF SL
Validated NT Sequence
ttattataaa gaaaaaatat tttttaaagt atcttttatt tctctataat acatttcttt tttagagggc tctaatcgtc catttataag aacttttata ctatttgaaa aatggatctt gcctttaata tattctctat attctttata aagcaagtcc tccacttctt ttatcgtgtt tctatttttt ataaactggt tttccacaat agaaatatta tatattttct ttctaaattt tgtaatatca ttaactgcgt taatcaaaat agtaaaactt tctattgacc atatttctac ctgaactgga atcacaatat aatccgcaac attcaaggca ttctttaaaa gaaaatctaa actaggagga gtatctaata aaacataatc aaaattatag ttttgcatat tttcatttaa atgatgttct aaaataactt ctttgtaatc aatggcttcc gaattaaatt ttcccaaaat ggggtgagag gggattatag atatataatc atcaattttt ttaatgcatt cattaaaatg gaaatctcct tttaacatac tatatgtatt atatttttca atatttgaaa tatatctatt aaaataagaa gttaaagaat tttgtggatc taaatcaatt agaagcacct ttttgcccaa ttcctttaat acataagaaa aaagtataga aagcacactt tttccgactc ctcccttaat tgaagccatt gttataatat ctgatttttt aatatccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDIKKSDII TMASIKGGVG KSVLSILFSY VLKELGKKVL LIDLDPQNSL TSYFNRYISN IEKYNTYSML KGDFHFNECI KKIDDYISII PSHPILGKFN SEAIDYKEVI LEHHLNENMQ NYNFDYVLLD TPPSLDFLLK NALNVADYIV IPVQVEIWSI ESFTILINAV NDITKFRKKI YNISIVENQF IKNRNTIKEV EDLLYKEYRE YIKGKIHFSN SIKVLINGRL EPSKKEMYYR EIKDTLKNIF SL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatattaaa aaatcagata ttataacaat ggcttcaatt aagggaggag tcggaaaaag tgtgctttct atactttttt cttatgtatt aaaggaattg ggcaaaaagg tgcttctaat tgatttagat ccacaaaatt ctttaacttc ttattttaat agatatattt caaatattga aaaatataat acatatagta tgttaaaagg agatttccat tttaatgaat gcattaaaaa aattgatgat tatatatcta taatcccctc tcaccccatt ttgggaaaat ttaattcgga agccattgat tacaaagaag ttattttaga acatcattta aatgaaaata tgcaaaacta taattttgat tatgttttat tagatactcc tcctagttta gattttcttt taaagaatgc cttgaatgtt gcggattata ttgtgattcc agttcaggta gaaatatggt caatagaaag ttttactatt ttgattaacg cagttaatga tattacaaaa tttagaaaga aaatatataa tatttctatt gtggaaaacc agtttataaa aaatagaaac acgataaaag aagtggagga cttgctttat aaagaatata gagaatatat taaaggcaag atccattttt caaatagtat aaaagttctt ataaatggac gattagagcc ctctaaaaaa gaaatgtatt atagagaaat aaaagatact ttaaaaaata ttttttcttt atagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BobuA.01182.b.B1.GE27145
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD IKKSDIITMA SIKGGVGKSV LSILFSYVLK ELGKKVLLID LDPQNSLTSY FNRYISNIEK YNTYSMLKGD FHFNECIKKI DDYISIIPSH PILGKFNSEA IDYKEVILEH HLNENMQNYN FDYVLLDTPP SLDFLLKNAL NVADYIVIPV QVEIWSIESF TILINAVNDI TKFRKKIYNI SIVENQFIKN RNTIKEVEDL LYKEYREYIK GKIHFSNSIK VLINGRLEPS KKEMYYREIK DTLKNIFSL
Validated NT Sequence
ataccatggc tcaccaccac caccaccata tggatattaa aaaatcagat attataacaa tggcttcaat taagggagga gtcggaaaaa gtgtgctttc tatacttttt tcttatgtat taaaggaatt gggcaaaaag gtgcttctaa ttgatttaga tccacaaaat tctttaactt cttattttaa tagatatatt tcaaatattg aaaaatataa tacatatagt atgttaaaag gagatttcca ttttaatgaa tgcattaaaa aaattgatga ttatatatct ataatcccct ctcaccccat tttgggaaaa tttaattcgg aagccattga ttacaaagaa gttattttag aacatcattt aaatgaaaat atgcaaaact ataattttga ttatgtttta ttagatactc ctcctagttt agattttctt ttaaagaatg ccttgaatgt tgcggattat attgtgattc cagttcaggt agaaatatgg tcaatagaaa gttttactat tttgattaac gcagttaatg atattacaaa atttagaaag aaaatatata atatttctat tgtggaaaac cagtttataa aaaatagaaa cacgataaaa gaagtggagg acttgcttta taaagaatat agagaatata ttaaaggcaa gatccatttt tcaaatagta taaaagttct tataaatgga cgattagagc cctctaaaaa agaaatgtat tatagagaaa taaaagatac tttaaaaaat attttttctt tataagtgag taaga
Expected Protein Sequence
MAHHHHHHMD IKKSDIITMA SIKGGVGKSV LSILFSYVLK ELGKKVLLID LDPQNSLTSY FNRYISNIEK YNTYSMLKGD FHFNECIKKI DDYISIIPSH PILGKFNSEA IDYKEVILEH HLNENMQNYN FDYVLLDTPP SLDFLLKNAL NVADYIVIPV QVEIWSIESF TILINAVNDI TKFRKKIYNI SIVENQFIKN RNTIKEVEDL LYKEYREYIK GKIHFSNSIK VLINGRLEPS KKEMYYREIK DTLKNIFSL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata ttaaaaaatc agatattata acaatggctt caattaaggg aggagtcgga aaaagtgtgc tttctatact tttttcttat gtattaaagg aattgggcaa aaaggtgctt ctaattgatt tagatccaca aaattcttta acttcttatt ttaatagata tatttcaaat attgaaaaat ataatacata tagtatgtta aaaggagatt tccattttaa tgaatgcatt aaaaaaattg atgattatat atctataatc ccctctcacc ccattttggg aaaatttaat tcggaagcca ttgattacaa agaagttatt ttagaacatc atttaaatga aaatatgcaa aactataatt ttgattatgt tttattagat actcctccta gtttagattt tcttttaaag aatgccttga atgttgcgga ttatattgtg attccagttc aggtagaaat atggtcaata gaaagtttta ctattttgat taacgcagtt aatgatatta caaaatttag aaagaaaata tataatattt ctattgtgga aaaccagttt ataaaaaata gaaacacgat aaaagaagtg gaggacttgc tttataaaga atatagagaa tatattaaag gcaagatcca tttttcaaat agtataaaag ttcttataaa tggacgatta gagccctcta aaaaagaaat gtattataga gaaataaaag atactttaaa aaatattttt tctttatagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat