BobuA.01180.a

xylose operon regulatory protein (xylR-2)

CENTER ID: BobuA.01180.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01180.a.A1.GE26482 Full length( BobuA.01180.a ) 1 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1223
RefSeq: NP_212965.1
UniProt: O51771

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRINMKHYLA IDIGGTSTKY SLSDSSGVFF DKNEISTGAT SDEQVNILVN IINSYKESSD IAGVAICIPG FVDLKGNVLR VNAISGFVNY PLKERLESLT GVSTEIENDA NCVALAEKFK GNAIDSNNFI AITLGTGIGA GIFANGKLLR GNSFMSGEVG FMITGGISNN IPFNCKWESI ASVSALRKRV AMRLGKPLKE VSGEFVFDLA ENGNIHAKNE VDRFFENLSF GIFNLTFILN PEKILIGGGI SARPDLIDRI YEKLENLWSL EMAFDNNNNI KNLVALEPTK FNNESGKIGA LFHYFTCKNQ NNTSF
NT Sequence
TTGAGGATAA ATATGAAACA TTATTTAGCG ATTGATATTG GCGGGACTAG TACCAAATAT TCGCTTTCAG ATTCAAGCGG TGTTTTTTTT GATAAAAATG AAATAAGCAC AGGTGCTACT TCTGACGAAC AAGTAAATAT TTTAGTTAAT ATTATTAATT CTTACAAAGA ATCAAGTGAT ATTGCGGGAG TTGCAATTTG TATTCCTGGG TTTGTTGATC TTAAGGGAAA TGTTCTTAGG GTAAATGCTA TTTCTGGATT TGTTAATTAT CCTTTAAAAG AGCGATTAGA ATCTTTAACC GGAGTAAGTA CAGAGATTGA AAATGATGCT AATTGTGTAG CCTTAGCAGA AAAATTTAAG GGTAATGCTA TTGACTCTAA TAATTTTATT GCTATAACTC TTGGCACAGG AATTGGTGCT GGAATTTTTG CAAATGGCAA GCTTTTAAGA GGAAATTCTT TTATGTCCGG AGAGGTTGGA TTTATGATTA CTGGAGGTAT TAGCAACAAT ATTCCCTTTA ATTGCAAATG GGAATCCATT GCGTCTGTTT CGGCTTTAAG AAAAAGAGTT GCTATGCGAT TAGGAAAACC TTTAAAAGAG GTTTCAGGAG AATTTGTTTT TGATCTAGCT GAGAATGGGA ATATTCATGC CAAAAATGAA GTTGATAGAT TTTTTGAGAA TTTGTCATTT GGTATTTTTA ATTTAACTTT TATTTTGAAT CCTGAAAAAA TTTTGATCGG AGGAGGAATA AGCGCAAGAC CCGATTTAAT AGATAGAATA TATGAAAAAT TAGAAAATTT ATGGTCTTTA GAAATGGCTT TTGATAATAA TAATAATATA AAAAATCTTG TAGCACTTGA GCCTACTAAA TTTAATAATG AATCTGGTAA AATTGGAGCT TTGTTTCATT ATTTTACTTG CAAAAATCAA AATAATACCT CATTT
Details for BobuA.01180.a.A1.GE26482
HARVESTED ON: 4/20/2009
SEQUENCED ON: 5/6/2009
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 47
Validated AA Sequence
MSGEVGFMIT GGISNNIPFN CKWESIASVS ALRKRVAMRL GKPLKEVSGE FVFDLAENGN IHAKNEVDRF FENLSFGIFN LTFILNPEKI LIGGGISARP DLIDRIYEKL ENLWSLEMAF DNNNNIKNLV ALEPTKFNNE SGKIGALFHY FTCKNQNNTS F
Validated NT Sequence
attaaaatga ggtattattt tgatttttgc aagtaaaata atgaaacaaa gctccaattt taccagattc attattaaat ttagtaggct caagtgctac aagatttttt atattattat tattatcaaa agccatttct aaagaccata aattttctaa tttttcatat attctatcta ttaaatcggg tcttgcgctt attcctcctc cgatcaaaat tttttcagga ttcaaaataa aagttaaatt aaaaatacca aatgacaaat tctcaaaaaa tctatcaact tcatttttgg catgaatatt cccattctca gctagatcaa aaacaaattc tcctgaaacc tcttttaaag gttttcctaa tcgcatagca actctttttc ttaaagccga aacagacgca atggattccc atttgcaatt aaagggaata ttgttgctaa tacctccagt aatcataaat ccaacctctc cggacataaa agaatttcct cttaaaagct tgccatttgc aaaaattcca gcaccaattc ctgtgccaag agttatagca ataaaattat tagagtcaat agcattaccc ttaaattttt ctgctaaggc tacacaatta gcatcatttt caatctctgt acttactccg gttaaagatt ctaatcgctc ttttaaagga taattaacaa atccagaaat agcatttacc ctaagaacat ttnccttaag atcaacaaac ccaggaatac aaattgcaac tcccgcaata tcacttgatt ctttgtaaga attaataata ttaactaaaa tatttacttg ntcgtcagaa ntngcacctg tgcttatttc atttttatca aaaaaacacc gct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLRINMKHY LAIDIGGTST KYSLSDSSGV FFDKNEISTG ATSDEQVNIL VNIINSYKES SDIAGVAICI PGFVDLKGNV LRVNAISGFV NYPLKERLES LTGVSTEIEN DANCVALAEK FKGNAIDSNN FIAITLGTGI GAGIFANGKL LRGNSFMSGE VGFMITGGIS NNIPFNCKWE SIASVSALRK RVAMRLGKPL KEVSGEFVFD LAENGNIHAK NEVDRFFENL SFGIFNLTFI LNPEKILIGG GISARPDLID RIYEKLENLW SLEMAFDNNN NIKNLVALEP TKFNNESGKI GALFHYFTCK NQNNTSF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaggata aatatgaaac attatttagc gattgatatt ggcgggacta gtaccaaata ttcgctttca gattcaagcg gtgttttttt tgataaaaat gaaataagca caggtgctac ttctgacgaa caagtaaata ttttagttaa tattattaat tcttacaaag aatcaagtga tattgcggga gttgcaattt gtattcctgg gtttgttgat cttaagggaa atgttcttag ggtaaatgct atttctggat ttgttaatta tcctttaaaa gagcgattag aatctttaac cggagtaagt acagagattg aaaatgatgc taattgtgta gccttagcag aaaaatttaa gggtaatgct attgactcta ataattttat tgctataact cttggcacag gaattggtgc tggaattttt gcaaatggca agcttttaag aggaaattct tttatgtccg gagaggttgg atttatgatt actggaggta ttagcaacaa tattcccttt aattgcaaat gggaatccat tgcgtctgtt tcggctttaa gaaaaagagt tgctatgcga ttaggaaaac ctttaaaaga ggtttcagga gaatttgttt ttgatctagc tgagaatggg aatattcatg ccaaaaatga agttgataga ttttttgaga atttgtcatt tggtattttt aatttaactt ttattttgaa tcctgaaaaa attttgatcg gaggaggaat aagcgcaaga cccgatttaa tagatagaat atatgaaaaa ttagaaaatt tatggtcttt agaaatggct tttgataata ataataatat aaaaaatctt gtagcacttg agcctactaa atttaataat gaatctggta aaattggagc tttgtttcat tattttactt gcaaaaatca aaataatacc tcattttaga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a