BobuA.01162.a

pyruvate kinase (BB_0348)

CENTER ID: BobuA.01162.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01162.a.A1.GE26480 Full length( BobuA.01162.a ) 1 477
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01162.a.A1.PS00260 Full length( BobuA.01162.a ) 1 477
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126685
PATRIC ID: fig|224326.49.peg.744
RefSeq: NP_212482.1
UniProt: O51323
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MISKLTKIVA TISDLRCEPE HIKDLHDAGV NVIRLNTAHQ SHEDTIKVID NVRKISNKIA LMIDTKGPEV RTANIENPII VKTGDKVIIS TSPINEPNNF QTNYDGFVKE VPQGSKVLID DGELEMTVVA KLPDRLICEI KNDGQIKNKK SINTPGISLK LQSVTEKDKG FIELAAKYNV DFIAHSFVRH SKDVQDVQEI LTASGNPDVK IISKIENQEG IDNIEEIAKA SYGIMVARGD MGVEIPAEDV PIAQLKITQT CIKYGIPVIT ATQMLHTMIE NPRPTRAEVS DIANAILNGT DAIMLSGETA YGKYPIEAVK MMTSIAKKVE KHRKMTLYKD ELFYDKSITR NYIIKCAIDA TKLMDIKAII VDSLKGKTAR IMATYRASVP LFITTNSERL ARELALSYGV YSNLVDNNFK RTTEFVVTSL KMLKEQGVVN DKDTVIIISG NPNRNIEKGT EFMEINTVED AIKGRNI
NT Sequence
ATGATTTCAA AGTTAACAAA AATTGTAGCA ACAATATCTG ATCTTAGATG CGAACCAGAA CATATAAAAG ATTTACACGA TGCAGGGGTA AATGTTATAA GACTAAATAC TGCTCATCAA TCACACGAAG ATACAATAAA AGTAATAGAC AATGTTAGAA AAATTTCAAA TAAAATAGCT TTAATGATTG ATACAAAAGG ACCAGAAGTT AGAACAGCAA ATATTGAAAA TCCTATTATT GTAAAAACTG GAGACAAGGT AATCATTTCA ACCTCGCCTA TTAATGAGCC TAATAACTTT CAAACCAATT ATGATGGATT TGTTAAAGAA GTACCCCAAG GATCTAAAGT GCTAATTGAT GACGGTGAAC TTGAAATGAC TGTTGTTGCC AAATTGCCTG ACAGATTAAT TTGTGAAATT AAAAATGACG GCCAAATTAA AAATAAAAAA TCAATCAATA CCCCCGGAAT TTCTCTTAAG CTACAATCAG TAACCGAAAA AGACAAAGGA TTTATTGAGC TTGCAGCAAA ATATAATGTT GATTTTATTG CCCATTCGTT TGTAAGACAT TCCAAAGACG TTCAAGATGT TCAAGAAATT TTAACTGCCT CTGGAAATCC TGATGTAAAA ATTATATCCA AAATCGAAAA TCAAGAGGGA ATTGACAACA TTGAAGAAAT TGCAAAAGCT TCTTACGGAA TAATGGTTGC AAGGGGAGAC ATGGGAGTTG AAATTCCTGC AGAAGATGTA CCCATTGCTC AACTTAAAAT AACACAAACC TGTATAAAGT ATGGAATACC CGTGATTACA GCAACTCAAA TGCTTCATAC AATGATTGAA AATCCAAGAC CTACTAGAGC AGAAGTGTCT GACATCGCTA ACGCTATTTT AAACGGCACA GACGCAATTA TGTTATCCGG AGAAACCGCC TACGGGAAAT ATCCAATTGA AGCTGTAAAA ATGATGACAA GCATTGCTAA AAAAGTTGAA AAACACAGAA AAATGACCTT ATATAAGGAT GAACTTTTTT ACGATAAAAG TATCACAAGA AACTATATTA TCAAATGTGC AATTGATGCC ACAAAACTTA TGGACATAAA AGCAATTATT GTAGATTCTC TAAAAGGCAA AACCGCAAGA ATAATGGCAA CCTACAGAGC AAGTGTTCCA TTATTCATTA CAACAAATAG CGAAAGACTA GCAAGAGAAT TAGCATTATC TTACGGGGTT TATTCTAATC TTGTAGACAA TAATTTCAAA AGAACTACCG AATTTGTAGT AACTTCTCTT AAAATGTTAA AAGAACAAGG TGTTGTTAAT GACAAAGATA CTGTAATAAT TATTTCTGGA AATCCAAATA GAAATATTGA AAAAGGAACA GAATTTATGG AAATAAACAC AGTAGAAGAT GCAATCAAAG GACGAAATAT A
Details for BobuA.01162.a.A1.GE26480
HARVESTED ON: 4/20/2009
SEQUENCED ON: 5/6/2009
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMISKLTKIV ATISDLRCEP EHIKDLHDAG VNVIRLNTAH QSHEDTIKVI DNVRKISNKI ALMIDTKGPE VRTANIENPI IVKTGDKVII STSPINEPNN FQTNYDGFVK EVPQGSKVLI DDGELEMTVV AKLPDRLICE IKNDGQIKNK KSINTPGISL KLQSVTEKDK GFIELAAKYN VDFIAHSFVR HSKDVQDVQE ILTASGNPDV KIISKIENQE GIDNIEEIAK ASYGIMVARG DMGVEIPAED VPIAQLKITQ TCIKYGIPVI TATQMLHTMI ENPRPTRAEV SDIANAILNG TDAIMLSGET AYGKYPIEAV KMMTSIAKKV EKHRKMTLYK DELFYDKSIT RNYIIKCAID ATKLMDIKAI IVDSLKGKTA RIMATYRASV PLFITTNSER LARELALSYG VYSNLVDNNF KRTTEFVVTS LKMLKEQGVV NDKDTVIIIS GNPNRNIEKG TEFMEINTVE DAIKGR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgattt caaagttaac aaaaattgta gcaacaatat ctgatcttag atgcgaacca gaacatataa aagatttaca cgatgcaggg gtaaatgtta taagactaaa tactgctcat caatcacacg aagatacaat aaaagtaata gacaatgtta gaaaaatttc aaataaaata gctttaatga ttgatacaaa aggaccagaa gttagaacag caaatattga aaatcctatt attgtaaaaa ctggagacaa ggtaatcatt tcaacctcgc ctattaatga gcctaataac tttcaaacca attatgatgg atttgttaaa gaagtacccc aaggatctaa agtgctaatt gatgacggtg aacttgaaat gactgttgtt gccaaattgc ctgacagatt aatttgtgaa attaaaaatg acggccaaat taaaaataaa aaatcaatca atacccccgg aatttctctt aagctacaat cagtaaccga aaaagacaaa ggatttattg agcttgcagc aaaatataat gttgatttta ttgcccattc gtttgtaaga cattccaaag acgttcaaga tgttcaagaa attttaactg cctctggaaa tcctgatgta aaaattatat ccaaaatcga aaatcaagag ggaattgaca acattgaaga aattgcaaaa gcttcttacg gaataatggt tgcaagggga gacatgggag ttgaaattcc tgcagaagat gtacccattg ctcaacttaa aataacacaa acctgtataa agtatggaat acccgtgatt acagcaactc aaatgcttca tacaatgatt gaaaatccaa gacctactag agcagaagtg tctgacatcg ctaacgctat tttaaacggc acagacgcaa ttatgttatc cggagaaacc gcctacggga aatatccaat tgaagctgta aaaatgatga caagcattgc taaaaaagtt gaaaaacaca gaaaaatgac cttatataag gatgaacttt tttacgataa aagtatcaca agaaactata ttatcaaatg tgcaattgat gccacaaaac ttatggacat aaaagcaatt attgtagatt ctctaaaagg caaaaccgca agaataatgg caacctacag agcaagtgtt ccattattca ttacaacaaa tagcgaaaga ctagcaagag aattagcatt atcttacggg gtttattcta atcttgtaga caataatttc aaaagaacta ccgaatttgt agtaacttct cttaaaatgt taaaagaaca aggtgttgtt aatgacaaag atactgtaat aattatttct ggaaatccaa atagaaatat tgaaaaagga acagaattta tggaaataaa cacagtagaa gatgcaatca aaggacgaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMISKLTKIV ATISDLRCEP EHIKDLHDAG VNVIRLNTAH QSHEDTIKVI DNVRKISNKI ALMIDTKGPE VRTANIENPI IVKTGDKVII STSPINEPNN FQTNYDGFVK EVPQGSKVLI DDGELEMTVV AKLPDRLICE IKNDGQIKNK KSINTPGISL KLQSVTEKDK GFIELAAKYN VDFIAHSFVR HSKDVQDVQE ILTASGNPDV KIISKIENQE GIDNIEEIAK ASYGIMVARG DMGVEIPAED VPIAQLKITQ TCIKYGIPVI TATQMLHTMI ENPRPTRAEV SDIANAILNG TDAIMLSGET AYGKYPIEAV KMMTSIAKKV EKHRKMTLYK DELFYDKSIT RNYIIKCAID ATKLMDIKAI IVDSLKGKTA RIMATYRASV PLFITTNSER LARELALSYG VYSNLVDNNF KRTTEFVVTS LKMLKEQGVV NDKDTVIIIS GNPNRNIEKG TEFMEINTVE DAIKGRNI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatttcaaag ttaacaaaaa ttgtagcaac aatatctgat cttagatgcg aaccagaaca tataaaagat ttacacgatg caggggtaaa tgttataaga ctaaatactg ctcatcaatc acacgaagat acaataaaag taatagacaa tgttagaaaa atttcaaata aaatagcttt aatgattgat acaaaaggac cagaagttag aacagcaaat attgaaaatc ctattattgt aaaaactgga gacaaggtaa tcatttcaac ctcgcctatt aatgagccta ataactttca aaccaattat gatggatttg ttaaagaagt accccaagga tctaaagtgc taattgatga cggtgaactt gaaatgactg ttgttgccaa attgcctgac agattaattt gtgaaattaa aaatgacggc caaattaaaa ataaaaaatc aatcaatacc cccggaattt ctcttaagct acaatcagta accgaaaaag acaaaggatt tattgagctt gcagcaaaat ataatgttga ttttattgcc cattcgtttg taagacattc caaagacgtt caagatgttc aagaaatttt aactgcctct ggaaatcctg atgtaaaaat tatatccaaa atcgaaaatc aagagggaat tgacaacatt gaagaaattg caaaagcttc ttacggaata atggttgcaa ggggagacat gggagttgaa attcctgcag aagatgtacc cattgctcaa cttaaaataa cacaaacctg tataaagtat ggaatacccg tgattacagc aactcaaatg cttcatacaa tgattgaaaa tccaagacct actagagcag aagtgtctga catcgctaac gctattttaa acggcacaga cgcaattatg ttatccggag aaaccgccta cgggaaatat ccaattgaag ctgtaaaaat gatgacaagc attgctaaaa aagttgaaaa acacagaaaa atgaccttat ataaggatga acttttttac gataaaagta tcacaagaaa ctatattatc aaatgtgcaa ttgatgccac aaaacttatg gacataaaag caattattgt agattctcta aaaggcaaaa ccgcaagaat aatggcaacc tacagagcaa gtgttccatt attcattaca acaaatagcg aaagactagc aagagaatta gcattatctt acggggttta ttctaatctt gtagacaata atttcaaaag aactaccgaa tttgtagtaa cttctcttaa aatgttaaaa gaacaaggtg ttgttaatga caaagatact gtaataatta tttctggaaa tccaaataga aatattgaaa aaggaacaga atttatggaa ataaacacag tagaagatgc aatcaaagga cgaaatatat aaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BobuA.01162.a.A1.PS00260
PURIFICATION DATe: 6/12/2009
CONCENTRATION: 31.35mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMISKLTKIV ATISDLRCEP EHIKDLHDAG VNVIRLNTAH QSHEDTIKVI DNVRKISNKI ALMIDTKGPE VRTANIENPI IVKTGDKVII STSPINEPNN FQTNYDGFVK EVPQGSKVLI DDGELEMTVV AKLPDRLICE IKNDGQIKNK KSINTPGISL KLQSVTEKDK GFIELAAKYN VDFIAHSFVR HSKDVQDVQE ILTASGNPDV KIISKIENQE GIDNIEEIAK ASYGIMVARG DMGVEIPAED VPIAQLKITQ TCIKYGIPVI TATQMLHTMI ENPRPTRAEV SDIANAILNG TDAIMLSGET AYGKYPIEAV KMMTSIAKKV EKHRKMTLYK DELFYDKSIT RNYIIKCAID ATKLMDIKAI IVDSLKGKTA RIMATYRASV PLFITTNSER LARELALSYG VYSNLVDNNF KRTTEFVVTS LKMLKEQGVV NDKDTVIIIS GNPNRNIEKG TEFMEINTVE DAIKGR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgattt caaagttaac aaaaattgta gcaacaatat ctgatcttag atgcgaacca gaacatataa aagatttaca cgatgcaggg gtaaatgtta taagactaaa tactgctcat caatcacacg aagatacaat aaaagtaata gacaatgtta gaaaaatttc aaataaaata gctttaatga ttgatacaaa aggaccagaa gttagaacag caaatattga aaatcctatt attgtaaaaa ctggagacaa ggtaatcatt tcaacctcgc ctattaatga gcctaataac tttcaaacca attatgatgg atttgttaaa gaagtacccc aaggatctaa agtgctaatt gatgacggtg aacttgaaat gactgttgtt gccaaattgc ctgacagatt aatttgtgaa attaaaaatg acggccaaat taaaaataaa aaatcaatca atacccccgg aatttctctt aagctacaat cagtaaccga aaaagacaaa ggatttattg agcttgcagc aaaatataat gttgatttta ttgcccattc gtttgtaaga cattccaaag acgttcaaga tgttcaagaa attttaactg cctctggaaa tcctgatgta aaaattatat ccaaaatcga aaatcaagag ggaattgaca acattgaaga aattgcaaaa gcttcttacg gaataatggt tgcaagggga gacatgggag ttgaaattcc tgcagaagat gtacccattg ctcaacttaa aataacacaa acctgtataa agtatggaat acccgtgatt acagcaactc aaatgcttca tacaatgatt gaaaatccaa gacctactag agcagaagtg tctgacatcg ctaacgctat tttaaacggc acagacgcaa ttatgttatc cggagaaacc gcctacggga aatatccaat tgaagctgta aaaatgatga caagcattgc taaaaaagtt gaaaaacaca gaaaaatgac cttatataag gatgaacttt tttacgataa aagtatcaca agaaactata ttatcaaatg tgcaattgat gccacaaaac ttatggacat aaaagcaatt attgtagatt ctctaaaagg caaaaccgca agaataatgg caacctacag agcaagtgtt ccattattca ttacaacaaa tagcgaaaga ctagcaagag aattagcatt atcttacggg gtttattcta atcttgtaga caataatttc aaaagaacta ccgaatttgt agtaacttct cttaaaatgt taaaagaaca aggtgttgtt aatgacaaag atactgtaat aattatttct ggaaatccaa atagaaatat tgaaaaagga acagaattta tggaaataaa cacagtagaa gatgcaatca aaggacgaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMISKLTKIV ATISDLRCEP EHIKDLHDAG VNVIRLNTAH QSHEDTIKVI DNVRKISNKI ALMIDTKGPE VRTANIENPI IVKTGDKVII STSPINEPNN FQTNYDGFVK EVPQGSKVLI DDGELEMTVV AKLPDRLICE IKNDGQIKNK KSINTPGISL KLQSVTEKDK GFIELAAKYN VDFIAHSFVR HSKDVQDVQE ILTASGNPDV KIISKIENQE GIDNIEEIAK ASYGIMVARG DMGVEIPAED VPIAQLKITQ TCIKYGIPVI TATQMLHTMI ENPRPTRAEV SDIANAILNG TDAIMLSGET AYGKYPIEAV KMMTSIAKKV EKHRKMTLYK DELFYDKSIT RNYIIKCAID ATKLMDIKAI IVDSLKGKTA RIMATYRASV PLFITTNSER LARELALSYG VYSNLVDNNF KRTTEFVVTS LKMLKEQGVV NDKDTVIIIS GNPNRNIEKG TEFMEINTVE DAIKGRNI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTTCAAAG TTAACAAAAA TTGTAGCAAC AATATCTGAT CTTAGATGCG AACCAGAACA TATAAAAGAT TTACACGATG CAGGGGTAAA TGTTATAAGA CTAAATACTG CTCATCAATC ACACGAAGAT ACAATAAAAG TAATAGACAA TGTTAGAAAA ATTTCAAATA AAATAGCTTT AATGATTGAT ACAAAAGGAC CAGAAGTTAG AACAGCAAAT ATTGAAAATC CTATTATTGT AAAAACTGGA GACAAGGTAA TCATTTCAAC CTCGCCTATT AATGAGCCTA ATAACTTTCA AACCAATTAT GATGGATTTG TTAAAGAAGT ACCCCAAGGA TCTAAAGTGC TAATTGATGA CGGTGAACTT GAAATGACTG TTGTTGCCAA ATTGCCTGAC AGATTAATTT GTGAAATTAA AAATGACGGC CAAATTAAAA ATAAAAAATC AATCAATACC CCCGGAATTT CTCTTAAGCT ACAATCAGTA ACCGAAAAAG ACAAAGGATT TATTGAGCTT GCAGCAAAAT ATAATGTTGA TTTTATTGCC CATTCGTTTG TAAGACATTC CAAAGACGTT CAAGATGTTC AAGAAATTTT AACTGCCTCT GGAAATCCTG ATGTAAAAAT TATATCCAAA ATCGAAAATC AAGAGGGAAT TGACAACATT GAAGAAATTG CAAAAGCTTC TTACGGAATA ATGGTTGCAA GGGGAGACAT GGGAGTTGAA ATTCCTGCAG AAGATGTACC CATTGCTCAA CTTAAAATAA CACAAACCTG TATAAAGTAT GGAATACCCG TGATTACAGC AACTCAAATG CTTCATACAA TGATTGAAAA TCCAAGACCT ACTAGAGCAG AAGTGTCTGA CATCGCTAAC GCTATTTTAA ACGGCACAGA CGCAATTATG TTATCCGGAG AAACCGCCTA CGGGAAATAT CCAATTGAAG CTGTAAAAAT GATGACAAGC ATTGCTAAAA AAGTTGAAAA ACACAGAAAA ATGACCTTAT ATAAGGATGA ACTTTTTTAC GATAAAAGTA TCACAAGAAA CTATATTATC AAATGTGCAA TTGATGCCAC AAAACTTATG GACATAAAAG CAATTATTGT AGATTCTCTA AAAGGCAAAA CCGCAAGAAT AATGGCAACC TACAGAGCAA GTGTTCCATT ATTCATTACA ACAAATAGCG AAAGACTAGC AAGAGAATTA GCATTATCTT ACGGGGTTTA TTCTAATCTT GTAGACAATA ATTTCAAAAG AACTACCGAA TTTGTAGTAA CTTCTCTTAA AATGTTAAAA GAACAAGGTG TTGTTAATGA CAAAGATACT GTAATAATTA TTTCTGGAAA TCCAAATAGA AATATTGAAA AAGGAACAGA ATTTATGGAA ATAAACACAG TAGAAGATGC AATCAAAGGA CGAAATATAT AAAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA