BobuA.01141.a

1-phosphofructokinase (EC 2.7.1.56) (Fructose 1-phosphate kinase) (BB_0630)

CENTER ID:	BobuA.01141.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01141.a.B1.GE39070	full length		1	307

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|224326.49.peg.1020
RefSeq:	NP_212764.1
UniProt:	O51575

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIYTLTLNPS VDYKIVLKEF QEESLNYALN NNFFAGGKGI NVSTVLKNLG KPSTALGFLG GFTGDYIRFS LDSRGIKNDF IKIKYDTRLN IKMIANGRET EINANSPDIS ENEFELLKNK LKNLANNSTL VMSGSVPAAL GEDAYNEIAN SISNDVKLII DTSGKPLRKI LRLNPFLIKP NIYELEDLFN AKFDSTKELI KIGKNLVESG VQNIIISMGS DGAIFIGGKN VAFRAFVPKI NFVSTIGAGD SVIAGFVYAF DNGSTLEDSF KFGVAAGTAT ALKGNLCEFQ DVKKMLCQIR VEDIYTS

NT Sequence

ttgatatata ctctaacact caatccttct gtggattata aaatagtttt aaaagaattt caggaagaaa gtcttaatta tgctttaaat aacaattttt ttgctggcgg taagggaata aatgtaagca ccgttcttaa aaatttagga aaacctagta cggctttggg atttttggga ggttttacgg gtgattatat aagattttct cttgattcta ggggcataaa aaacgatttt attaaaataa aatatgatac aagattaaat attaaaatga tagcaaatgg cagagaaaca gaaattaatg ccaattctcc agatatttct gagaatgaat ttgaactttt gaaaaataaa cttaaaaatt tagcaaataa tagtacatta gtgatgtcag gaagtgttcc cgcagctctt ggtgaggatg catacaatga aatagctaat agcatttcta atgatgttaa gcttatcatt gataccagtg gcaaaccttt gcgaaaaatt cttagattaa atcccttttt aataaagcct aatatttatg aacttgaaga tctttttaat gctaaatttg attctacaaa agaattgatt aaaatcggaa aaaatcttgt agaaagtggg gttcaaaaca ttataatttc catgggaagt gacggagcta tttttattgg cggcaaaaat gttgctttta gggcctttgt tcctaagatt aattttgtta gcaccattgg agcaggagac tctgtgattg ccgggtttgt atatgctttt gataatggaa gtactttaga ggattcattt aaatttggtg ttgcagctgg tacagctacg gcattaaaag gcaatctttg tgaatttcaa gatgttaaaa agatgctttg tcagattagg gttgaggata tttacacttc ttaa

Details for BobuA.01141.a.B1.GE39070

HARVESTED ON:	3/31/2015
SEQUENCED ON:	3/27/2015
EXPECTED MW:	35kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	96
PERCENT COVERAGE:	93

Validated AA Sequence

MAHHHHHHML IYTLTLNPSV DYKIVLKEFQ EESLNYALNN NFFAGGKGIN VSTVLKNLGK PSTALGFLGG FTGDYIRFSL DSRGIKNDFN KIKYDTRLNI KMIANGRETE INANSPDISE NEFELLKNKL KNLANNSTLV MSGSVPAALG EDAYNEIANS ISNDVKLIID TSGKPLRKIL RLNPFLIKPN IYELEDLFNA KFDSTKELIK IGKNLVESGV QNIIISMGSD GAIFIGGKNV AFRAFVPKIN FVSTIGAGDS VIAGFVYAFD NGSTLEDSFK FGVAAGSYGI KRQSL

Validated NT Sequence

atcttactca cttaagaagt gtaaatatcc tcaaccctaa tctgacaaag catcttttta acatcttgaa attcacaaag attgcctttt aatgccgtag ctaccagctg caacaccaaa tttaaatgaa tcctctaaag tacttccatt atcaaaagca tatacaaacc cggcaatcac agagtctcct gctccaatgg tgctaacaaa attaatctta ggaacaaagg ccctaaaagc aacatttttg ccgccaataa aaatagctcc gtcacttccc atggaaatta taatgttttg aaccccactt tctacaagat tttttccgat tttaatcaat tcttttgtag aatcaaattt agcattaaaa agatcttcaa gttcataaat attaggcttt attaaaaagg gatttaatct aagaattttt cgcaaaggtt tgccactggt atcaatgata agcttaacat cattagaaat gctattagct atttcattgt atgcatcctc accaagagct gcgggaacac ttcctgacat cactaatgta ctattatttg ctaaattttt aagtttattt ttcaaaagtt caaattcatt ctcagaaata tctggagaat tggcattaat ttctgtttct ctgccatttg ctatcatttt aatatttaat cttgtatcat attttatttt attaaaatcg ttttttatgc ccctagaatc aagagaaaat cttatataat cacccgtaaa acctcccaaa aatcccaaag ccgtactagg ttttcctaaa tttttaagaa cggtgcttac atttattccc ttaccgccag caaaaaaatt gttatttaaa gcataattaa gactttcttc ctgaaattct tttaaaacta ttttataatc cacagaagga ttgagtgtta gagtatatat caacatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHMI YTLTLNPSVD YKIVLKEFQE ESLNYALNNN FFAGGKGINV STVLKNLGKP STALGFLGGF TGDYIRFSLD SRGIKNDFIK IKYDTRLNIK MIANGRETEI NANSPDISEN EFELLKNKLK NLANNSTLVM SGSVPAALGE DAYNEIANSI SNDVKLIIDT SGKPLRKILR LNPFLIKPNI YELEDLFNAK FDSTKELIKI GKNLVESGVQ NIIISMGSDG AIFIGGKNVA FRAFVPKINF VSTIGAGDSV IAGFVYAFDN GSTLEDSFKF GVAAGTATAL KGNLCEFQDV KKMLCQIRVE DIYTS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgatat atactctaac actcaatcct tctgtggatt ataaaatagt tttaaaagaa tttcaggaag aaagtcttaa ttatgcttta aataacaatt tttttgctgg cggtaaggga ataaatgtaa gcaccgttct taaaaattta ggaaaaccta gtacggcttt gggatttttg ggaggtttta cgggtgatta tataagattt tctcttgatt ctaggggcat aaaaaacgat tttattaaaa taaaatatga tacaagatta aatattaaaa tgatagcaaa tggcagagaa acagaaatta atgccaattc tccagatatt tctgagaatg aatttgaact tttgaaaaat aaacttaaaa atttagcaaa taatagtaca ttagtgatgt caggaagtgt tcccgcagct cttggtgagg atgcatacaa tgaaatagct aatagcattt ctaatgatgt taagcttatc attgatacca gtggcaaacc tttgcgaaaa attcttagat taaatccctt tttaataaag cctaatattt atgaacttga agatcttttt aatgctaaat ttgattctac aaaagaattg attaaaatcg gaaaaaatct tgtagaaagt ggggttcaaa acattataat ttccatggga agtgacggag ctatttttat tggcggcaaa aatgttgctt ttagggcctt tgttcctaag attaattttg ttagcaccat tggagcagga gactctgtga ttgccgggtt tgtatatgct tttgataatg gaagtacttt agaggattca tttaaatttg gtgttgcagc tggtacagct acggcattaa aaggcaatct ttgtgaattt caagatgtta aaaagatgct ttgtcagatt agggttgagg atatttacac ttcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat