BobuA.01121.a

4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis protein (thiJ) (BB_0621)

CENTER ID:	BobuA.01121.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01121.a.A1.GE26477	Full length( BobuA.01121.a )		1	184

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127669
PATRIC ID:	fig\|224326.49.peg.1011
RefSeq:	NP_212755.1
UniProt:	O51566

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVVGIILANG FEDIEAIIPI DILRRGNVNI QIISTNDSNV VISSKGVSFL ADDIISNCKE NCFDLIILPG GMPGATNLFN SKELDLILKD MNSKGKFIAA ICASPVVVLA AKGLLGFNKF TCYPGLEKNV LDGEFVDENV VRSNNFITSK GVGTSFEFAF TLLEMVKGKQ IMEDVKKATL LCKS

NT Sequence

ATGGTAGTAG GAATTATTCT TGCAAATGGC TTTGAAGATA TTGAGGCCAT AATCCCGATT GATATTTTAA GACGGGGTAA TGTTAATATT CAAATTATCA GCACAAATGA TAGCAATGTT GTGATAAGTT CAAAAGGCGT TTCTTTTTTA GCAGATGATA TAATATCAAA CTGTAAGGAA AATTGTTTTG ATCTAATAAT TCTCCCGGGA GGTATGCCTG GAGCTACTAA TCTTTTTAAT TCAAAAGAAT TGGATTTGAT TTTAAAAGAT ATGAATTCCA AAGGTAAGTT TATTGCAGCT ATTTGTGCTT CTCCGGTAGT AGTGCTTGCT GCTAAAGGTC TTCTAGGATT CAATAAGTTT ACATGTTATC CAGGTTTGGA GAAAAATGTG CTTGATGGTG AGTTTGTAGA TGAAAATGTT GTTAGAAGCA ATAATTTTAT TACTTCTAAA GGAGTTGGAA CTTCATTTGA ATTTGCTTTT ACTCTTCTTG AAATGGTAAA AGGAAAACAA ATAATGGAAG ATGTTAAAAA AGCAACTTTG CTTTGCAAGA GT

Details for BobuA.01121.a.A1.GE26477

HARVESTED ON:	4/20/2009
SEQUENCED ON:	5/6/2009
EXPECTED MW:	22kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVVGIILAN GFEDIEAIIP IDILRRGNVN IQIISTNDSN VVISSKGVSF LADDIISNCK ENCFDLIILP GGMPGATNLF NSKELDLILK DMNSKGKFIA AICASPVVVL AAKGLLGFNK FTCYPGLEKN VLDGEFVDEN VVRSNNFITS KGVGTSFEFA FTLLEMVKGK QIMEDVKKAT LLC

Validated NT Sequence

tgcaaagcaa agttgctttt ttaacatctt ccattatttg ttttcctttt accatttcaa gaagagtaaa agcaaattca aatgaagttc caactccttt agaagtaata aaattattgc ttctaacaac attttcatct acaaactcac catcaagcac atttttctcc aaacctggat aacatgtaaa cttattgaat cctagaagac ctttagcagc aagcactact accggagaag cacaaatagc tgcaataaac ttacctttgg aattcatatc ttttaaaatc aaatccaatt cttttgaatt aaaaagatta gtagctccag gcatacctcc cgggagaatt attagatcaa aacaattttc cttacagttt gatattatat catctgctaa aaaagaaacg ccttttgaac ttatcacaac attgctatca tttgtgctga taatttgaat attaacatta ccccgtctta aaatatcaat cgggattatg gcctcaatat cttcaaagcc atttgcaaga ataattccta ctaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtagtagga attattcttg caaatggctt tgaagatatt gaggccataa tcccgattga tattttaaga cggggtaatg ttaatattca aattatcagc acaaatgata gcaatgttgt gataagttca aaaggcgttt cttttttagc agatgatata atatcaaact gtaaggaaaa ttgttttgat ctaataattc tcccgggagg tatgcctgga gctactaatc tttttaattc aaaagaattg gatttgattt taaaagatat gaattccaaa ggtaagttta ttgcagctat ttgtgcttct ccggtagtag tgcttgctgc taaaggtctt ctaggattca ataagtttac atgttatcca ggtttggaga aaaatgtgct tgatggtgag tttgtagatg aaaatgttgt tagaagcaat aattttatta cttctaaagg agttggaact tcatttgaat ttgcttttac tcttcttgaa atggtaaaag gaaaacaaat aatggaagat gttaaaaaag caactttgct ttgcaagagt taaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa