BobuA.01059.a

methionyl-tRNAformyl transferase (fmt) (BB_0064)

CENTER ID: BobuA.01059.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01059.a.B2.GE39069 mature protein 17 312
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.462
RefSeq: NP_212198.1
UniProt: O51091

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIFFVSSSS IALEVFKEIV KHYEVVGVLT LPDRPKGRGQ KLSQNVIKSE AIARNIKVLD PLILDDNVLN LVRDLNPDLM LVFSYGKIFK KEFLDLFPKG CINVHPSLLP KYRGVSPIQS AILNGDCVSG VTIQSMALEM DSGNILVQKN FKIRSYDTSH DISKLVSSLS PSLVLEALEK ISKGFLGIPQ KSSEATFCSF LKKESGFIDF NLSAFEIKNK INACNPWPLV RVRLDYNDII FHRADFLEVD LYKERKIGEI VDFNPEKGLF VNTGKGILLL LEVQRPGRKV LDFKSFYNGS RQLIGQVFSS IE
NT Sequence
atgaaaatat tttttgtaag ctcttcttct attgctttag aagttttcaa agagattgta aagcattatg aagtggtggg agttttgact ttgcctgata gacccaaggg tagaggacaa aagttaagtc aaaatgtaat aaagtcagag gctattgcta ggaatattaa agttcttgat cctttgattc ttgatgataa tgtattaaat ttagttagag atttgaatcc agatttaatg ttagtttttt cttatggcaa gatttttaaa aaagaatttt tagatctttt cccaaaggga tgtattaatg ttcatccttc tcttttaccc aaatatagag gcgtttctcc gattcaatct gcaattttaa atggtgattg tgttagcggt gttactattc agagtatggc tttagaaatg gatagtggca atattttagt gcaaaaaaat tttaaaataa gatcttatga tacaagtcat gatatttcaa aacttgtatc aagccttagt ccaagccttg ttctagaagc tttggaaaaa attagcaagg gttttttggg aattcctcaa aaatcaagcg aagctacttt ttgttctttt ttaaaaaaag aatctggatt tatagatttt aatttaagtg catttgagat taaaaataaa attaatgcgt gcaatccttg gccacttgta agagttaggc ttgattataa cgacattatt tttcatcgcg ccgatttttt ggaggtagat ttatataagg agagaaaaat aggagaaatt gttgatttta atcctgaaaa aggattattt gttaataccg ggaaagggat tcttttgctt ttagaagttc aaagacctgg aagaaaggtt ttggatttta agtcctttta taatggaagt aggcagctaa tagggcaagt gttttcttca atagaatag
Details for BobuA.01059.a.B2.GE39069
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK EIVKHYEVVG VLTLPDRPKG RGQKLSQNVI KSEAIARNIK VLDPLILDDN VLNLVRDLNP DLMLVFSYGK IFKKEFLDLF PKGCINVHPS LLPKYRGVSP IQSAILNGDC VSGVTIQSMA LEMDSGNILV QKNFKIRSYD TSHDISKLVS SLSPSLVLEA LEKISKGFLG IPQKSSEATF CSFLKKESGF IDFNLSAFEI KNKINACNPW PLVRVRLDYN DIIFHRADFL EVDLYKERKI GEIVDFNPEK GLFVNTGKGI LLLLEVQRPG RKVLDFKSFY NGSRQLIGQV FSSIE
Validated NT Sequence
ttactcactt attctattga agaaaacact tgccctatta gctgcctact tccattataa aaggacttaa aatccaaaac ctttcttcca ggtctttgaa cttctaaaag caaaagaatc cctttcccgg tattaacaaa taatcctttt tcaggattaa aatcaacaat ttctcctatt tttctctcct tatataaatc tacctccaaa aaatcggcgc gatgaaaaat aatgtcgtta taatcaagcc taactcttac aagtggccaa ggattgcacg cattaatttt atttttaatc tcaaatgcac ttaaattaaa atctataaat ccagattctt tttttaaaaa agaacaaaaa gtagcttcgc ttgatttttg aggaattccc aaaaaaccct tgctaatttt ttccaaagct tctagaacaa ggcttggact aaggcttgat acaagttttg aaatatcatg acttgtatca taagatctta ttttaaaatt tttttgcact aaaatattgc cactatccat ttctaaagcc atactctgaa tagtaacacc gctaacacaa tcaccattta aaattgcaga ttgaatcgga gaaacgcctc tatatttggg taaaagagaa ggatgaacat taatacatcc ctttgggaaa agatctaaaa attctttttt aaaaatcttg ccataagaaa aaactaacat taaatctgga ttcaaatctc taactaaatt taatacatta tcatcaagaa tcaaaggatc aagaacttta atattcctag caatagcctc tgactttatt acattttgac ttaacttttg tcctctaccc ttgggtctat caggcaaagt caaaactccc accacttcat aatgctttac aatctctttc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHKE IVKHYEVVGV LTLPDRPKGR GQKLSQNVIK SEAIARNIKV LDPLILDDNV LNLVRDLNPD LMLVFSYGKI FKKEFLDLFP KGCINVHPSL LPKYRGVSPI QSAILNGDCV SGVTIQSMAL EMDSGNILVQ KNFKIRSYDT SHDISKLVSS LSPSLVLEAL EKISKGFLGI PQKSSEATFC SFLKKESGFI DFNLSAFEIK NKINACNPWP LVRVRLDYND IIFHRADFLE VDLYKERKIG EIVDFNPEKG LFVNTGKGIL LLLEVQRPGR KVLDFKSFYN GSRQLIGQVF SSIE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaagaga ttgtaaagca ttatgaagtg gtgggagttt tgactttgcc tgatagaccc aagggtagag gacaaaagtt aagtcaaaat gtaataaagt cagaggctat tgctaggaat attaaagttc ttgatccttt gattcttgat gataatgtat taaatttagt tagagatttg aatccagatt taatgttagt tttttcttat ggcaagattt ttaaaaaaga atttttagat cttttcccaa agggatgtat taatgttcat ccttctcttt tacccaaata tagaggcgtt tctccgattc aatctgcaat tttaaatggt gattgtgtta gcggtgttac tattcagagt atggctttag aaatggatag tggcaatatt ttagtgcaaa aaaattttaa aataagatct tatgatacaa gtcatgatat ttcaaaactt gtatcaagcc ttagtccaag ccttgttcta gaagctttgg aaaaaattag caagggtttt ttgggaattc ctcaaaaatc aagcgaagct actttttgtt cttttttaaa aaaagaatct ggatttatag attttaattt aagtgcattt gagattaaaa ataaaattaa tgcgtgcaat ccttggccac ttgtaagagt taggcttgat tataacgaca ttatttttca tcgcgccgat tttttggagg tagatttata taaggagaga aaaataggag aaattgttga ttttaatcct gaaaaaggat tatttgttaa taccgggaaa gggattcttt tgcttttaga agttcaaaga cctggaagaa aggttttgga ttttaagtcc ttttataatg gaagtaggca gctaataggg caagtgtttt cttcaataga atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat