BobuA.01042.a

aldose reductase, putative

CENTER ID: BobuA.01042.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01042.a.A1.GE26476 Full length( BobuA.01042.a ) 1 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.918
RefSeq: NP_212662.1
UniProt: O51478

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNNLKDKINT YSKLILGSWQ FGGGYFKQVE KETAKKILKK AYDHGIRNID TARAYGNGIS EKIIGEIIEK DPTIRENILI ASKCYPMEIS EYRENFNESL KNLKTDYIDI YYIHWPKADF DLRPIASFLE EMRVKGRIKY VGVSNFEISH MESIKKVCKI DVNQIGYNPL FRNKEKDVIP YCEDNNIATI SYSTIAQGLL SKANIKDKNK FNDIRTEKLI LFKKEIWPYT LKTINKLEEI AKINNLTILE LTYSWLKKTK LSGFIVGFSK ENYVESNVNS FKAEINDKVY EEITSILDNF NHQTKNFPNL FNKKI
NT Sequence
GTGAATAATC TTAAAGACAA GATAAATACT TATAGCAAAT TAATTTTAGG GTCTTGGCAA TTCGGAGGAG GATATTTTAA GCAAGTCGAA AAAGAAACTG CTAAAAAAAT ATTAAAAAAA GCATATGATC ACGGGATCAG AAATATTGAT ACTGCAAGAG CTTATGGAAA TGGAATTTCG GAAAAAATAA TTGGCGAAAT AATAGAAAAA GATCCAACAA TAAGAGAAAA TATTTTAATT GCAAGTAAAT GCTACCCAAT GGAAATTTCA GAATATAGAG AGAACTTTAA TGAAAGTCTT AAAAATTTAA AAACTGATTA TATAGATATT TACTATATAC ACTGGCCTAA AGCCGATTTT GACCTAAGAC CAATCGCATC ATTTCTTGAA GAAATGAGAG TAAAAGGAAG AATAAAATAT GTAGGCGTAA GTAATTTTGA AATATCACAC ATGGAAAGCA TAAAAAAAGT TTGCAAAATT GACGTAAACC AAATAGGATA CAACCCCTTA TTTAGAAATA AAGAAAAAGA TGTAATTCCT TACTGTGAAG ACAACAATAT TGCCACCATA TCATATTCAA CAATTGCTCA AGGACTTTTA TCTAAAGCTA ATATAAAAGA CAAAAACAAA TTTAATGATA TTAGAACAGA AAAATTGATA CTTTTCAAAA AAGAAATTTG GCCTTATACT TTGAAAACCA TAAATAAACT TGAAGAGATA GCAAAGATAA ATAACTTAAC AATTTTAGAA TTAACATATT CATGGCTTAA AAAAACAAAA TTAAGTGGAT TTATAGTGGG CTTTAGCAAG GAAAATTATG TAGAATCAAA CGTAAATTCA TTTAAAGCAG AAATTAATGA TAAAGTATAT GAAGAGATTA CATCAATTTT AGATAATTTC AATCACCAAA CAAAAAACTT CCCAAATTTA TTTAACAAAA AAATT
Details for BobuA.01042.a.A1.GE26476
HARVESTED ON: 4/20/2009
SEQUENCED ON: 5/6/2009
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MVNNLKDKIN TYSKLILGSW QFGGGYFKQV EKETAKKILK KAYDHGIRNI DTARAYGNGI SEKIIGEIIE KDPTIRENIL IASKCYPMEI SEYRENFNES LKNLKTDYID IYYIHWPKAD FDLRPIASFL EEMRVKGRIK YVGVSNFEIS HMESIKKVCK IDVNQIGYNP LFRNKEKDVI PYCEDNNIAT ISYSTIAQGL LSKANIKDKN KFNDIRTEKL ILFKKEIWPY TLKTINKLEE IAKINNLTIL ELTYSWLKKT KLSGFIVGFS KENYVESNVN SFKAEINDKV YEEITSILDN FNHQTKNFPN LFNK
Validated NT Sequence
ttttgttaaa taaatttggg aagttttttg tttggtgatt gaaattatct aaaattgatg taatctcttc atatacttta tcattaattt cngctttaaa tgaatttacg tttgattcta cataattttc cttgctaaag cccactataa atccacttaa ttttgttttt ttaagccatg aatatgttaa ttctaaaatt gttaagttat ttatctttgc tatctcttca agtttattta tggttttcaa agtataaggc caaatttctt ttttgaaaag tatcaatttt tctgttctaa tatcattaaa tttgtttttg tcttttatat tagctttaga taaaagtcct tgagcaattg ttgaatatga tatggtggca atattgttgt cttcacagta aggaattaca tctttttctt tatttctaaa taaggggttg tatcctattt ggtttacgtc aattttgcaa acttttttta tgctttccat gtgtgatatt tcaaaattac ttacgcctac atattttatt cttcctttta ctctcatttc ttcaagaaat gatgcgattg gtcttaggtc aaaatcggct ttaggccagt gtatatagta aatatctata taatcagttt ttaaattttt aagactttca ttaaagttct ctctatattc tgaaatttcc attgggtagc atttacttgc aattaaaata ttttctctta ttgttggatc tttttctatt atttcgccaa ttattttttc cgaaattcca tttccataag ctcttgcagt atcaatattt ctgatcccgt gatcatatgc tttttttaat atttttttag cagtttcttt ttcgacttgc ttaaaatatc ctcctccgaa ttgccaagac cctaaaatta atttgctata agtatttatc ttgtctttaa gattattcac catcgaacca ggaccctggg tctgagcttc caggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNNLKDKI NTYSKLILGS WQFGGGYFKQ VEKETAKKIL KKAYDHGIRN IDTARAYGNG ISEKIIGEII EKDPTIRENI LIASKCYPME ISEYRENFNE SLKNLKTDYI DIYYIHWPKA DFDLRPIASF LEEMRVKGRI KYVGVSNFEI SHMESIKKVC KIDVNQIGYN PLFRNKEKDV IPYCEDNNIA TISYSTIAQG LLSKANIKDK NKFNDIRTEK LILFKKEIWP YTLKTINKLE EIAKINNLTI LELTYSWLKK TKLSGFIVGF SKENYVESNV NSFKAEINDK VYEEITSILD NFNHQTKNFP NLFNKKI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaataat cttaaagaca agataaatac ttatagcaaa ttaattttag ggtcttggca attcggagga ggatatttta agcaagtcga aaaagaaact gctaaaaaaa tattaaaaaa agcatatgat cacgggatca gaaatattga tactgcaaga gcttatggaa atggaatttc ggaaaaaata attggcgaaa taatagaaaa agatccaaca ataagagaaa atattttaat tgcaagtaaa tgctacccaa tggaaatttc agaatataga gagaacttta atgaaagtct taaaaattta aaaactgatt atatagatat ttactatata cactggccta aagccgattt tgacctaaga ccaatcgcat catttcttga agaaatgaga gtaaaaggaa gaataaaata tgtaggcgta agtaattttg aaatatcaca catggaaagc ataaaaaaag tttgcaaaat tgacgtaaac caaataggat acaacccctt atttagaaat aaagaaaaag atgtaattcc ttactgtgaa gacaacaata ttgccaccat atcatattca acaattgctc aaggactttt atctaaagct aatataaaag acaaaaacaa atttaatgat attagaacag aaaaattgat acttttcaaa aaagaaattt ggccttatac tttgaaaacc ataaataaac ttgaagagat agcaaagata aataacttaa caattttaga attaacatat tcatggctta aaaaaacaaa attaagtgga tttatagtgg gctttagcaa ggaaaattat gtagaatcaa acgtaaattc atttaaagca gaaattaatg ataaagtata tgaagagatt acatcaattt tagataattt caatcaccaa acaaaaaact tcccaaattt atttaacaaa aaaatttaaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a