BobuA.01032.a

tyrosyl-tRNA synthetase (tyrS) (BB_0370)

CENTER ID: BobuA.01032.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01032.a.A1.GE26521 Full length( BobuA.01032.a ) 1 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01032.a.A1.PS00229 Full length( BobuA.01032.a ) 1 405
BobuA.01032.a.A1.PW27583 Full length( BobuA.01032.a ) 1 405
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127413
PATRIC ID: fig|224326.49.peg.765
RefSeq: NP_212504.1
UniProt: O51343
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLALSLLHK RGFLKQCTSL KVLSDLMDRE KIVFYAGVDA TSSSLHIGHL IPFLAMMHLR QHGHMPIVLI GDSTAKIGDP SGKSEMRKIL SSEEIGNNAL LIKNQLQRIT KFTSECFIHN SNWLDNLNYI EFLRDVGMHF SVNRMLSFET YKRRMDFGLS FIEFNYQLLQ SYDYYMLNKI KNCRLQIGGD DQWGNIISGV DLIRKKNGSE TFGLTFPLIT RSDGKKMGKS EKGAVYLDSN LFSIYDFYQY FRNTSDSDVK TFLYLFTFLE EDEIELISNF KGNSLNKAKE ILAFEITKIV HGEAEALKVQ EASFAAFRGS GDRSNIPFFK FSFSSLKEEI LLVDLMLDSK IVPSKSEGRR LIDSGGVYIN GKRVESQSHL LTKKDFNNNE VELRVGKKKF LRIVI
NT Sequence
ATGAATCTTG CTTTAAGTCT TTTACATAAA CGCGGATTTT TAAAGCAATG TACATCTTTA AAAGTTTTAA GTGATTTAAT GGATAGGGAA AAAATAGTTT TTTATGCAGG AGTTGATGCA ACATCTAGTT CTCTTCATAT TGGCCATTTG ATTCCCTTTT TAGCAATGAT GCATCTTAGG CAACACGGGC ACATGCCAAT TGTTTTGATT GGAGATTCTA CAGCAAAAAT AGGCGATCCT TCTGGAAAAA GTGAGATGAG AAAGATTTTA TCTTCAGAAG AGATTGGCAA TAATGCTTTG TTGATAAAAA ATCAACTTCA AAGAATAACT AAGTTTACTT CAGAATGTTT TATTCATAAT TCAAATTGGT TAGATAATCT CAATTATATT GAATTTTTAA GAGATGTTGG CATGCATTTT TCTGTTAATC GTATGTTAAG CTTTGAAACT TATAAAAGAA GGATGGATTT CGGACTTTCA TTTATTGAGT TTAATTATCA ACTTTTGCAG TCTTATGATT ATTATATGCT TAATAAAATT AAAAATTGCC GACTTCAAAT TGGTGGTGAT GATCAATGGG GGAATATTAT TTCAGGGGTT GACCTAATTA GAAAAAAAAA TGGATCAGAA ACTTTTGGGC TTACTTTTCC ATTAATTACA AGAAGTGATG GAAAAAAGAT GGGTAAATCA GAAAAAGGCG CTGTTTATCT TGATTCTAAT CTTTTTAGTA TTTATGATTT TTATCAGTAT TTTAGAAATA CTTCAGATTC TGATGTGAAA ACTTTTTTAT ATCTTTTTAC TTTTTTAGAA GAAGATGAGA TTGAATTAAT TTCAAATTTT AAGGGGAATT CTTTAAATAA GGCCAAAGAG ATTTTGGCTT TTGAGATAAC TAAAATTGTT CACGGAGAGG CAGAAGCCTT GAAAGTTCAA GAGGCATCTT TTGCCGCATT TAGGGGAAGT GGAGATAGGA GCAATATTCC ATTTTTTAAA TTTAGCTTTT CTAGCCTAAA AGAAGAGATA TTATTGGTTG ATTTAATGTT AGATTCAAAA ATTGTGCCCA GCAAATCAGA AGGCAGAAGA TTGATTGATT CTGGAGGTGT TTATATTAAT GGTAAAAGGG TAGAAAGTCA GAGTCACCTT CTTACCAAAA AGGATTTTAA TAACAATGAA GTTGAATTAA GAGTAGGTAA AAAAAAATTT TTACGAATTG TTATA
Details for BobuA.01032.a.A1.GE26521
HARVESTED ON: 4/20/2009
SEQUENCED ON: 5/6/2009
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNLALSLLH KRGFLKQCTS LKVLSDLMDR EKIVFYAGVD ATSSSLHIGH LIPFLAMMHL RQHGHMPIVL IGDSTAKIGD PSGKSEMRKI LSSEEIGNNA LLIKNQLQRI TKFTSECFIH NSNWLDNLNY IEFLRDVGMH FSVNRMLSFE TYKRRMDFGL SFIEFNYQLL QSYDYYMLNK IKNCRLQIGG DDQWGNIISG VDLIRKKNGS ETFGLTFPLI TRSDGKKMGK SEKGAVYLDS NLFSIYDFYQ YFRNTSDSDV KTFLYLFTFL EEDEIELISN FKGNSLNKAK EILAFEITKI VHGEAEALKV QEASFAAFRG SGDRSNIPFF KFSFSSLKEE ILLVDLMLDS KIVPSKSEGR RLIDSGGVYI NGKRVESQSH LLTKKDFNNN EVELRVGKKK FLRIVI
Validated NT Sequence
ttattatata acaattcgta aaaatttttt tttacctact cttaattcaa cttcattgtt attaaaatcc tttttggtaa gaaggtgact ctgactttct acccttttac cattaatata aacacctcca gaatcaatca atcttctgcc ttctgatttg ctgggcacaa tttttgaatc taacattaaa tcaaccaata atatctcttc ttttaggcta gaaaagctaa atttaaaaaa tggaatattg ctcctatctc cacttcccct aaatgcggca aaagatgcct cttgaacttt caaggcttct gcctctccgt gaacaatttt agttatctca aaagccaaaa tctctttggc cttatttaaa gaattcccct taaaatttga aattaattca atctcatctt cttctaaaaa agtaaaaaga tataaaaaag ttttcacatc agaatctgaa gtatttctaa aatactgata aaaatcataa atactaaaaa gattagaatc aagataaaca gcgccttttt ctgatttacc catctttttt ccatcacttc ttgtaattaa tggaaaagta agcccaaaag tttctgatcc attttttttt ctaattaggt caacccctga aataatattc ccccattgat catcaccacc aatttgaagt cggcaatttt taattttatt aagcatataa taatcataag actgcaaaag ttgataatta aactcaataa atgaaagtcc gaaatccatc cttcttttat aagtttcaaa gcttaacata cgattaacag aaaaatgcat gccaacatct cttaaaaatt caatataatt gagattatct aaccaatttg aattatgaat aaaacattct gaagtaaact tagttattct ttgaagttga ttttttatca acaaagcatt attgccaatc tcttctgaag ataaaatctt tctcatctca ctttttccag aaggatcgcc tatttttgct gtagaatctc caatcaaaac aattggcatg tgcccgtgtt gcctaagatg catcattgct aaaaagggaa tcaaatggcc aatatgaaga gaactagatg ttgcatcaac tcctgcataa aaaactattt tttccctatc cattaaatca cttaaaactt ttaaagatgt acattgcttt aaaaatccgc gtttatgtaa aagacttaaa gcaagattca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLALSLLH KRGFLKQCTS LKVLSDLMDR EKIVFYAGVD ATSSSLHIGH LIPFLAMMHL RQHGHMPIVL IGDSTAKIGD PSGKSEMRKI LSSEEIGNNA LLIKNQLQRI TKFTSECFIH NSNWLDNLNY IEFLRDVGMH FSVNRMLSFE TYKRRMDFGL SFIEFNYQLL QSYDYYMLNK IKNCRLQIGG DDQWGNIISG VDLIRKKNGS ETFGLTFPLI TRSDGKKMGK SEKGAVYLDS NLFSIYDFYQ YFRNTSDSDV KTFLYLFTFL EEDEIELISN FKGNSLNKAK EILAFEITKI VHGEAEALKV QEASFAAFRG SGDRSNIPFF KFSFSSLKEE ILLVDLMLDS KIVPSKSEGR RLIDSGGVYI NGKRVESQSH LLTKKDFNNN EVELRVGKKK FLRIVI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatcttgct ttaagtcttt tacataaacg cggattttta aagcaatgta catctttaaa agttttaagt gatttaatgg atagggaaaa aatagttttt tatgcaggag ttgatgcaac atctagttct cttcatattg gccatttgat tcccttttta gcaatgatgc atcttaggca acacgggcac atgccaattg ttttgattgg agattctaca gcaaaaatag gcgatccttc tggaaaaagt gagatgagaa agattttatc ttcagaagag attggcaata atgctttgtt gataaaaaat caacttcaaa gaataactaa gtttacttca gaatgtttta ttcataattc aaattggtta gataatctca attatattga atttttaaga gatgttggca tgcatttttc tgttaatcgt atgttaagct ttgaaactta taaaagaagg atggatttcg gactttcatt tattgagttt aattatcaac ttttgcagtc ttatgattat tatatgctta ataaaattaa aaattgccga cttcaaattg gtggtgatga tcaatggggg aatattattt caggggttga cctaattaga aaaaaaaatg gatcagaaac ttttgggctt acttttccat taattacaag aagtgatgga aaaaagatgg gtaaatcaga aaaaggcgct gtttatcttg attctaatct ttttagtatt tatgattttt atcagtattt tagaaatact tcagattctg atgtgaaaac ttttttatat ctttttactt ttttagaaga agatgagatt gaattaattt caaattttaa ggggaattct ttaaataagg ccaaagagat tttggctttt gagataacta aaattgttca cggagaggca gaagccttga aagttcaaga ggcatctttt gccgcattta ggggaagtgg agataggagc aatattccat tttttaaatt tagcttttct agcctaaaag aagagatatt attggttgat ttaatgttag attcaaaaat tgtgcccagc aaatcagaag gcagaagatt gattgattct ggaggtgttt atattaatgg taaaagggta gaaagtcaga gtcaccttct taccaaaaag gattttaata acaatgaagt tgaattaaga gtaggtaaaa aaaaattttt acgaattgtt atatagaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BobuA.01032.a.A1.PS00229
PURIFICATION DATe: 6/10/2009
CONCENTRATION: 22.98mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNLALSLLH KRGFLKQCTS LKVLSDLMDR EKIVFYAGVD ATSSSLHIGH LIPFLAMMHL RQHGHMPIVL IGDSTAKIGD PSGKSEMRKI LSSEEIGNNA LLIKNQLQRI TKFTSECFIH NSNWLDNLNY IEFLRDVGMH FSVNRMLSFE TYKRRMDFGL SFIEFNYQLL QSYDYYMLNK IKNCRLQIGG DDQWGNIISG VDLIRKKNGS ETFGLTFPLI TRSDGKKMGK SEKGAVYLDS NLFSIYDFYQ YFRNTSDSDV KTFLYLFTFL EEDEIELISN FKGNSLNKAK EILAFEITKI VHGEAEALKV QEASFAAFRG SGDRSNIPFF KFSFSSLKEE ILLVDLMLDS KIVPSKSEGR RLIDSGGVYI NGKRVESQSH LLTKKDFNNN EVELRVGKKK FLRIVI
Validated NT Sequence
ttattatata acaattcgta aaaatttttt tttacctact cttaattcaa cttcattgtt attaaaatcc tttttggtaa gaaggtgact ctgactttct acccttttac cattaatata aacacctcca gaatcaatca atcttctgcc ttctgatttg ctgggcacaa tttttgaatc taacattaaa tcaaccaata atatctcttc ttttaggcta gaaaagctaa atttaaaaaa tggaatattg ctcctatctc cacttcccct aaatgcggca aaagatgcct cttgaacttt caaggcttct gcctctccgt gaacaatttt agttatctca aaagccaaaa tctctttggc cttatttaaa gaattcccct taaaatttga aattaattca atctcatctt cttctaaaaa agtaaaaaga tataaaaaag ttttcacatc agaatctgaa gtatttctaa aatactgata aaaatcataa atactaaaaa gattagaatc aagataaaca gcgccttttt ctgatttacc catctttttt ccatcacttc ttgtaattaa tggaaaagta agcccaaaag tttctgatcc attttttttt ctaattaggt caacccctga aataatattc ccccattgat catcaccacc aatttgaagt cggcaatttt taattttatt aagcatataa taatcataag actgcaaaag ttgataatta aactcaataa atgaaagtcc gaaatccatc cttcttttat aagtttcaaa gcttaacata cgattaacag aaaaatgcat gccaacatct cttaaaaatt caatataatt gagattatct aaccaatttg aattatgaat aaaacattct gaagtaaact tagttattct ttgaagttga ttttttatca acaaagcatt attgccaatc tcttctgaag ataaaatctt tctcatctca ctttttccag aaggatcgcc tatttttgct gtagaatctc caatcaaaac aattggcatg tgcccgtgtt gcctaagatg catcattgct aaaaagggaa tcaaatggcc aatatgaaga gaactagatg ttgcatcaac tcctgcataa aaaactattt tttccctatc cattaaatca cttaaaactt ttaaagatgt acattgcttt aaaaatccgc gtttatgtaa aagacttaaa gcaagattca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLALSLLH KRGFLKQCTS LKVLSDLMDR EKIVFYAGVD ATSSSLHIGH LIPFLAMMHL RQHGHMPIVL IGDSTAKIGD PSGKSEMRKI LSSEEIGNNA LLIKNQLQRI TKFTSECFIH NSNWLDNLNY IEFLRDVGMH FSVNRMLSFE TYKRRMDFGL SFIEFNYQLL QSYDYYMLNK IKNCRLQIGG DDQWGNIISG VDLIRKKNGS ETFGLTFPLI TRSDGKKMGK SEKGAVYLDS NLFSIYDFYQ YFRNTSDSDV KTFLYLFTFL EEDEIELISN FKGNSLNKAK EILAFEITKI VHGEAEALKV QEASFAAFRG SGDRSNIPFF KFSFSSLKEE ILLVDLMLDS KIVPSKSEGR RLIDSGGVYI NGKRVESQSH LLTKKDFNNN EVELRVGKKK FLRIVI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATCTTGCT TTAAGTCTTT TACATAAACG CGGATTTTTA AAGCAATGTA CATCTTTAAA AGTTTTAAGT GATTTAATGG ATAGGGAAAA AATAGTTTTT TATGCAGGAG TTGATGCAAC ATCTAGTTCT CTTCATATTG GCCATTTGAT TCCCTTTTTA GCAATGATGC ATCTTAGGCA ACACGGGCAC ATGCCAATTG TTTTGATTGG AGATTCTACA GCAAAAATAG GCGATCCTTC TGGAAAAAGT GAGATGAGAA AGATTTTATC TTCAGAAGAG ATTGGCAATA ATGCTTTGTT GATAAAAAAT CAACTTCAAA GAATAACTAA GTTTACTTCA GAATGTTTTA TTCATAATTC AAATTGGTTA GATAATCTCA ATTATATTGA ATTTTTAAGA GATGTTGGCA TGCATTTTTC TGTTAATCGT ATGTTAAGCT TTGAAACTTA TAAAAGAAGG ATGGATTTCG GACTTTCATT TATTGAGTTT AATTATCAAC TTTTGCAGTC TTATGATTAT TATATGCTTA ATAAAATTAA AAATTGCCGA CTTCAAATTG GTGGTGATGA TCAATGGGGG AATATTATTT CAGGGGTTGA CCTAATTAGA AAAAAAAATG GATCAGAAAC TTTTGGGCTT ACTTTTCCAT TAATTACAAG AAGTGATGGA AAAAAGATGG GTAAATCAGA AAAAGGCGCT GTTTATCTTG ATTCTAATCT TTTTAGTATT TATGATTTTT ATCAGTATTT TAGAAATACT TCAGATTCTG ATGTGAAAAC TTTTTTATAT CTTTTTACTT TTTTAGAAGA AGATGAGATT GAATTAATTT CAAATTTTAA GGGGAATTCT TTAAATAAGG CCAAAGAGAT TTTGGCTTTT GAGATAACTA AAATTGTTCA CGGAGAGGCA GAAGCCTTGA AAGTTCAAGA GGCATCTTTT GCCGCATTTA GGGGAAGTGG AGATAGGAGC AATATTCCAT TTTTTAAATT TAGCTTTTCT AGCCTAAAAG AAGAGATATT ATTGGTTGAT TTAATGTTAG ATTCAAAAAT TGTGCCCAGC AAATCAGAAG GCAGAAGATT GATTGATTCT GGAGGTGTTT ATATTAATGG TAAAAGGGTA GAAAGTCAGA GTCACCTTCT TACCAAAAAG GATTTTAATA ACAATGAAGT TGAATTAAGA GTAGGTAAAA AAAAATTTTT ACGAATTGTT ATATAGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for BobuA.01032.a.A1.PW27583
PURIFICATION DATe: 9/22/2009
CONCENTRATION: 6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 25
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNLALSLLH KRGFLKQCTS LKVLSDLMDR EKIVFYAGVD ATSSSLHIGH LIPFLAMMHL RQHGHMPIVL IGDSTAKIGD PSGKSEMRKI LSSEEIGNNA LLIKNQLQRI TKFTSECFIH NSNWLDNLNY IEFLRDVGMH FSVNRMLSFE TYKRRMDFGL SFIEFNYQLL QSYDYYMLNK IKNCRLQIGG DDQWGNIISG VDLIRKKNGS ETFGLTFPLI TRSDGKKMGK SEKGAVYLDS NLFSIYDFYQ YFRNTSDSDV KTFLYLFTFL EEDEIELISN FKGNSLNKAK EILAFEITKI VHGEAEALKV QEASFAAFRG SGDRSNIPFF KFSFSSLKEE ILLVDLMLDS KIVPSKSEGR RLIDSGGVYI NGKRVESQSH LLTKKDFNNN EVELRVGKKK FLRIVI
Validated NT Sequence
ttattatata acaattcgta aaaatttttt tttacctact cttaattcaa cttcattgtt attaaaatcc tttttggtaa gaaggtgact ctgactttct acccttttac cattaatata aacacctcca gaatcaatca atcttctgcc ttctgatttg ctgggcacaa tttttgaatc taacattaaa tcaaccaata atatctcttc ttttaggcta gaaaagctaa atttaaaaaa tggaatattg ctcctatctc cacttcccct aaatgcggca aaagatgcct cttgaacttt caaggcttct gcctctccgt gaacaatttt agttatctca aaagccaaaa tctctttggc cttatttaaa gaattcccct taaaatttga aattaattca atctcatctt cttctaaaaa agtaaaaaga tataaaaaag ttttcacatc agaatctgaa gtatttctaa aatactgata aaaatcataa atactaaaaa gattagaatc aagataaaca gcgccttttt ctgatttacc catctttttt ccatcacttc ttgtaattaa tggaaaagta agcccaaaag tttctgatcc attttttttt ctaattaggt caacccctga aataatattc ccccattgat catcaccacc aatttgaagt cggcaatttt taattttatt aagcatataa taatcataag actgcaaaag ttgataatta aactcaataa atgaaagtcc gaaatccatc cttcttttat aagtttcaaa gcttaacata cgattaacag aaaaatgcat gccaacatct cttaaaaatt caatataatt gagattatct aaccaatttg aattatgaat aaaacattct gaagtaaact tagttattct ttgaagttga ttttttatca acaaagcatt attgccaatc tcttctgaag ataaaatctt tctcatctca ctttttccag aaggatcgcc tatttttgct gtagaatctc caatcaaaac aattggcatg tgcccgtgtt gcctaagatg catcattgct aaaaagggaa tcaaatggcc aatatgaaga gaactagatg ttgcatcaac tcctgcataa aaaactattt tttccctatc cattaaatca cttaaaactt ttaaagatgt acattgcttt aaaaatccgc gtttatgtaa aagacttaaa gcaagattca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLALSLLH KRGFLKQCTS LKVLSDLMDR EKIVFYAGVD ATSSSLHIGH LIPFLAMMHL RQHGHMPIVL IGDSTAKIGD PSGKSEMRKI LSSEEIGNNA LLIKNQLQRI TKFTSECFIH NSNWLDNLNY IEFLRDVGMH FSVNRMLSFE TYKRRMDFGL SFIEFNYQLL QSYDYYMLNK IKNCRLQIGG DDQWGNIISG VDLIRKKNGS ETFGLTFPLI TRSDGKKMGK SEKGAVYLDS NLFSIYDFYQ YFRNTSDSDV KTFLYLFTFL EEDEIELISN FKGNSLNKAK EILAFEITKI VHGEAEALKV QEASFAAFRG SGDRSNIPFF KFSFSSLKEE ILLVDLMLDS KIVPSKSEGR RLIDSGGVYI NGKRVESQSH LLTKKDFNNN EVELRVGKKK FLRIVI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATCTTGCT TTAAGTCTTT TACATAAACG CGGATTTTTA AAGCAATGTA CATCTTTAAA AGTTTTAAGT GATTTAATGG ATAGGGAAAA AATAGTTTTT TATGCAGGAG TTGATGCAAC ATCTAGTTCT CTTCATATTG GCCATTTGAT TCCCTTTTTA GCAATGATGC ATCTTAGGCA ACACGGGCAC ATGCCAATTG TTTTGATTGG AGATTCTACA GCAAAAATAG GCGATCCTTC TGGAAAAAGT GAGATGAGAA AGATTTTATC TTCAGAAGAG ATTGGCAATA ATGCTTTGTT GATAAAAAAT CAACTTCAAA GAATAACTAA GTTTACTTCA GAATGTTTTA TTCATAATTC AAATTGGTTA GATAATCTCA ATTATATTGA ATTTTTAAGA GATGTTGGCA TGCATTTTTC TGTTAATCGT ATGTTAAGCT TTGAAACTTA TAAAAGAAGG ATGGATTTCG GACTTTCATT TATTGAGTTT AATTATCAAC TTTTGCAGTC TTATGATTAT TATATGCTTA ATAAAATTAA AAATTGCCGA CTTCAAATTG GTGGTGATGA TCAATGGGGG AATATTATTT CAGGGGTTGA CCTAATTAGA AAAAAAAATG GATCAGAAAC TTTTGGGCTT ACTTTTCCAT TAATTACAAG AAGTGATGGA AAAAAGATGG GTAAATCAGA AAAAGGCGCT GTTTATCTTG ATTCTAATCT TTTTAGTATT TATGATTTTT ATCAGTATTT TAGAAATACT TCAGATTCTG ATGTGAAAAC TTTTTTATAT CTTTTTACTT TTTTAGAAGA AGATGAGATT GAATTAATTT CAAATTTTAA GGGGAATTCT TTAAATAAGG CCAAAGAGAT TTTGGCTTTT GAGATAACTA AAATTGTTCA CGGAGAGGCA GAAGCCTTGA AAGTTCAAGA GGCATCTTTT GCCGCATTTA GGGGAAGTGG AGATAGGAGC AATATTCCAT TTTTTAAATT TAGCTTTTCT AGCCTAAAAG AAGAGATATT ATTGGTTGAT TTAATGTTAG ATTCAAAAAT TGTGCCCAGC AAATCAGAAG GCAGAAGATT GATTGATTCT GGAGGTGTTT ATATTAATGG TAAAAGGGTA GAAAGTCAGA GTCACCTTCT TACCAAAAAG GATTTTAATA ACAATGAAGT TGAATTAAGA GTAGGTAAAA AAAAATTTTT ACGAATTGTT ATATAGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA